GSM1436993[Accession] - GEO DataSets

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Items: 3

Select item 2000594461.

Human Peripheral blood mononuclear cells (PBMCs): Control vs. Kashin-Beck Disease

(Submitter supplied) Objective:To identify an accurate blood-based gene signature for early detection of Kashin-Beck disease (KBD). Methods: Gene expression analysis was conducted of peripheral blood samples from 100 patients with KBD and 100 controls randomly chosen from two KBD-endemic areas

Organism:: Homo sapiens

Type:: Expression profiling by array

Platform:: GPL18887
200 Samples

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Accession:: GSE59446

ID:: 200059446

Analyze with GEO2R

Select item 1000188872.

NERCMDSNU Homo sapiens 15K version 1.0

(Submitter supplied) 1. Use the primer 5.0 to design probes and test them with oligo6.0 software. The principles of probe design as follows:(1) the length of probe is 59 bases, and annealing temperature is 78.0±5.°C, (2) tandem duplication bases was not more than 6,(3) the stem structure of stem loop in the internal of probes was not more than 6bases, (4) the most stable secondary structure base pairing of probe molecule was less than 6bp, (5) the Score value is less than 40, after comparing the probes with non-targeting gene sequence, which means the serial homology fragments were not more than 20 bases. more...