GEO DataSets

(Submitter supplied) At human body temperature, the fungal pathogen Candida albicans can transition from yeast to filamentous morphologies in response to host-relevant cues. Additionally, elevated temperatures encountered during febrile episodes can independently induce C. albicans filamentation. However, the underlying genetic pathways governing this developmental transition in response to elevated temperature remain largely unexplored. more...

Organism:

Candida albicans

Type:

Expression profiling by high throughput sequencing

Platform:

GPL28323

21 Samples

Download data: TDF, TXT

(Submitter supplied) Candida auris has emerged as a significant healthcare-associated pathogen, posing a serious challenge due to its multidrug-resistant nature. Given the pre-existing constraints in the discovery and provision of new antifungals, there is thus an urgent imperative to design effective strategies to tackle this pressing global concern. Here, we screened a chemical library and identified phenyl-carbohydrazide derivatives with potent activity against both C. more...

Organism:

Candida albicans SC5314; [Candida] auris

Type:

Expression profiling by high throughput sequencing; Other

Platforms:

GPL33190 GPL28368 GPL34459

24 Samples

Download data: TXT

(Submitter supplied) Candida albicansis the leading cause of life-threatening bloodstream candidiasis, especially among immunocompromised patients. The reversible morphological transition from yeast to hyphal filaments in response to host environmental cues facilitatesC.albicanstissue invasion, immune evasion, and dissemination. Hence, it is widely considered that filamentation represents one of the major virulence properties inC.albicans. more...

Organism:

Candida albicans

Type:

Expression profiling by high throughput sequencing

Platform:

GPL33213

8 Samples

Download data: TXT

(Submitter supplied) tRNA modifications play important roles in maintaining translation accuracy in all domains of life. Disruptions in the tRNA modification machinery, especially of the anticodon stem loop, can be lethal for many bacteria and lead to a broad range of phenotypes in baker’s yeast. Very little is known about the function of tRNA modifications in host-pathogen interactions, where rapidly changing environments and stresses require fast adaptations. more...

Organism:

Candida albicans

Type:

Other

Platform:

GPL22403

12 Samples

Download data: TXT

(Submitter supplied) Candida albicansis a commensal yeast that has important impacts on host metabolism and immune function, and can establish life-threatening infections in immunocompromised individuals. Previously,C. albicanscolonization has been shown to contribute to the progression and severity of alcoholic liver disease. However, relatively little is known about howC. albicansresponds to changing environmental conditions in the GI tract of individuals with alcohol use disorder, namely repeated exposure to ethanol. more...

Organism:

Candida albicans SC5314

Type:

Expression profiling by high throughput sequencing

Platform:

GPL33778

24 Samples

Download data: TXT

(Submitter supplied) The mammalian gut secretes a family of multifunctional peptides that affect appetite, intestinal secretions, and motility, while some regulate the microbiota. We report peptide YY (PYY1-36), but not endocrine PYY3-36, is an antimicrobial peptide (AMP) expressed by gut epithelial Paneth Cells (PC). PC-PYY has limited antibacterial activity, but shows selective activity against virulent hyphal, but not yeast forms, of Candida albicans. more...

Organism:

Candida albicans

Type:

Expression profiling by high throughput sequencing

Platform:

GPL28323

12 Samples

Download data: CSV

(Submitter supplied) The Hxk1 protein of Candida albicans is a moonlighting protein with a wide variety of functions. It phosphorylates N-acetylglucosamine (GlcNAc) which is necessary for different purposes in the cell including energy and chitin production. Further, this protein is a regulator for morphogenesis independent of its function during GlcNAc catabolism.To identify the regulatory role of Hxk1 and putative downstream regulator Hxk2, we performed CUT&RUN experiments for GFP-tagged Hxk1 strains and GFP-tagged Hxk2 strains.

Organism:

Candida albicans

Type:

Genome binding/occupancy profiling by high throughput sequencing

Platform:

GPL22403

12 Samples

Download data: NARROWPEAK

(Submitter supplied) As transition metal availability is very limited inside the human host, fungal pathogens have evolved sophisticated mechanisms to uptake and utilize these micronutrients at the infection interface. While considerable attention was turned into iron, copper and zinc acquisition mechanisms and their importance in fungal fitness, less was done regarding either the role of Mn in infectious processes or the cellular mechanism by which fungal cells achieve their Mn-homeostasis. more...

Organism:

Candida albicans

Type:

Expression profiling by high throughput sequencing

Platform:

GPL28323

12 Samples

Download data: TXT

(Submitter supplied) To characterize the interaction of Candida albicans with intestinal epithelial cells (C2BBe1) from early fungal adhesion to invasion (up to 6 h) and the late translocation and damage phase (12 -24 h), we conducted dual RNA-sequencing of C. albicans-infected C2BBe1 cells over a 24 h time course, with sampling at 0 h, 45 min, 3 h, 6 h, 12 h, and 24 h.

Organism:

Homo sapiens; Candida albicans

Type:

Expression profiling by high throughput sequencing

Platforms:

GPL20301 GPL18448

51 Samples

Download data: XLS

(Submitter supplied) Clinical isolates of the human fungal pathogen Candida albicans show significant variation in their ability to undergo in vitro filamentation. In this study, we show that Nrg1, a key repressor of filamentation and filament specific gene expression in standard laboratory strain, has strain dependent functions, especially during infection.

Organism:

Candida albicans

Type:

Other

Platform:

GPL34110

44 Samples

Download data: RCC, XLSX

(Submitter supplied) Our genetic screen reveals that deletion of CTM1, which abolishes the lysine trimethylation of cytochrome c (Cyc1), results in inhibition of hyphal morphogenesis in Candida albicans. Similar results are observed in the unmethylatable Cyc1 mutant (cyc1K79A). To elucidate how unmethylated Cyc1 inhibits hyphal growth, we performed RNA-Seq analysis by comparing WT (BWP17), ctm1∆/∆, and cyc1K79A cells grown in yeast and hyphal condition. more...

Organism:

Candida albicans SC5314

Type:

Expression profiling by high throughput sequencing

Platform:

GPL33780

27 Samples

Download data: XLSX

(Submitter supplied) Yeasts are naturally diverse, genetically tractable, and easy to grow such that researchers can investigate any number of genotypes, environments, or interactions thereof. However, studies of yeast transcriptomes have been limited by the processing capabilities of traditional RNA sequencing techniques. Here we optimize a powerful, high-throughput single-cell RNA sequencing (scRNAseq) platform, SPLiT-seq (Split Pool Ligation-based Transcriptome sequencing), for yeasts and apply it to 43,388 cells of multiple species and ploidies. more...

Organism:

Candida albicans; Saccharomyces cerevisiae

Type:

Expression profiling by high throughput sequencing

Platforms:

GPL26171 GPL34036

7 Samples

Download data: MTX, TSV

(Submitter supplied) In order to get an insight into the complex interplay of mRNAs during the growth in complete human serum supplemented with butanol, the total RNA population of Candida albiacns NCCPF 400034 extracted at 20, 45, and 120 minutes was sequenced by Next Generation sequencing techniques. The control RNA was extracted from Candida cells placed in YPD medium for 18 hrs at 37 ° C and was also sequenced using the next generation sequenicng technique.

Organism:

Candida albicans

Type:

Expression profiling by high throughput sequencing

Platforms:

GPL15149 GPL28323

8 Samples

Download data: XLSX

(Submitter supplied) In order to get an insight into the complex interplay of mRNAs during the growth in complete human serum, the mRNA polulation of Candida albiacns NCCPF 400034 taken at 0, 20, 45, and 120 minutes was extracted and sequenced by Next Generation sequencing techniques.

Organism:

Candida albicans

Type:

Expression profiling by high throughput sequencing

Platforms:

GPL15149 GPL15645

8 Samples

Download data: XLSX

(Submitter supplied) The regulatory factor X (RFX) gene family comprises a functionally diverse group of transcription factors characterized by a highly conserved and unique winged-helix DNA binding domain. The RFX proteins control a variety of processes ranging from genome integrity and cell cycle in model yeasts to immune functions and tissue differentiation in higher eukaryotes. The yeast Candida albicans encodes two RFX homologs, one of which (CR_06110C, termed RFX1) remains uncharacterized. more...

Organism:

Candida albicans

Type:

Expression profiling by high throughput sequencing

Platform:

GPL28323

6 Samples

Download data: TXT

(Submitter supplied) Project Abstract : Paf1C is a general transcription regulator and is associated with various human diseases. One of its subunits, CTR9, is known as a structurally and functionally core component of Paf1C. While its role in higher eukaryotes have been researched, the association between the core component and human fungal pathogen is not fully understood yet. During pre-infection process, Candida albicans adheres to the surface of hosts and sufficiently grows as the circular form. more...

Organism:

Candida albicans

Type:

Expression profiling by high throughput sequencing

Platform:

GPL19036

12 Samples

Download data: BW

(Submitter supplied) The ability of the fungus Candida albicans to filament and form biofilms contributes to its burden as a leading cause of hospital-acquired infections. Biofilm development involves an interconnected transcriptional regulatory network (TRN) consisting of nine transcription factors (TFs) that bind both to their own regulatory regions and to those of the other network TFs. Here, we show that seven of the nine TFs in the C. more...

Organism:

Candida albicans

Type:

Expression profiling by high throughput sequencing

Platform:

GPL28323

12 Samples

Download data: CSV, TXT

(Submitter supplied) In dinoflagellates, the most unique and divergent nuclear organization among the known diversity of eukaryotes has evolved. The list of highly unusual features of dinoflagellate nuclei and genomes is long -- permanently condensed liquid crystalline chromosomes, in which histones are not the main packaging component, genes organized as very long unidirectional gene arrays, general absence of transcriptional regulation, high abundance of the otherwise very rare DNA modification 5-hydroxymethyluracil (5-hmU), and many others. more...

Organism:

Candida albicans; Homo sapiens; Bigelowiella natans; Breviolum minutum; Saccharomyces cerevisiae

Type:

Genome binding/occupancy profiling by high throughput sequencing; Methylation profiling by high throughput sequencing; Other

4 related Platforms

34 Samples

Download data: BEDGRAPH, BIGWIG

(Submitter supplied) Fungal pathogens threaten ecosystems and human health. Understanding the molecular basis of their virulence is key to develop new treatment strategies. Here, we characterize NCS2*, a point mutation identified in a clinical baker's yeast isolate. Ncs2 is essential for 2-thiolation of tRNA and the NCS2* mutation leads to increased thiolation at body temperature. NCS2* yeast exhibits enhanced fitness when grown at elevated temperatures or when exposed to oxidative stress, inhibition of nutrient signalling, and cell-wall stress. more...

Organism:

Candida albicans

Type:

Other

Platform:

GPL22403

12 Samples

Download data: TXT

(Submitter supplied) Expression of 68 genes was tracked across different C. albicans strains and enviornmenal conditions using a custom 72 probe Nanostring library

Organism:

Candida albicans

Type:

Expression profiling by array

Platform:

GPL33714

76 Samples

Download data: RCC, XLSX