GEO DataSets

(Submitter supplied) SAGE was used to profile gene expression in leukemic myeloid progenitor cells of AML patients with t(8;21), t(15;17), t(9;11) and inv(16). Keywords: SAGE analysis of gene expression in leukemic myeloid progenitor cells isolated from bone marrow

Organism:

Homo sapiens

Type:

Expression profiling by SAGE

Platform:

GPL4

22 Samples

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(Submitter supplied) We performed a genome-wide analysis of gene expression in primary human CD151 myeloid progenitor cells. By using the serial analysis of gene expression (SAGE) technique, we obtained quantitative information for the expression of 37,519 unique SAGE-tag sequences. Of these unique tags, (i) 25% were detected at high and intermediate levels, whereas 75% were present as single copies, (ii) 53% of the tags matched known expressed sequences, 34% of which were matched to more than one known expressed sequence, and (iii) 47% of the tags had no matches and represent potentially novel genes. more...

Organism:

Homo sapiens

Type:

Expression profiling by SAGE

Platform:

GPL4

1 Sample

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(Submitter supplied) Acute myeloid leukemia (AML) is a heterogeneous group of diseases. Normal cytogenetics (CN) constitutes the single largest group, while trisomy 8 (+8) as a sole abnormality is the most frequent trisomy. How trisomy contributes to tumorigenesis is unknown. We used oligonucleotide-based DNA microarrays to study global gene expression in AML+8 patients with +8 as the sole chromosomal abnormality and AML-CN patients. more...

Organism:

Homo sapiens

Type:

Expression profiling by array

Platform:

GPL80

31 Samples

Download data: CEL

(Submitter supplied) Gene expression profile of acute myeloid leukemia. Bone marrow (BM) samples from 43 adult patients with newly de novo diagnosed AML.All samples contained more than 80% blast cells. Total RNA was extracted using Trizol reagent (Life Technologies, Gaithersburg, MD) and purified with RNeasy Mini Kit (Quiagen, Valencia, CA). The RNA integrity was assessed using Agilent 2100 Bioanalyzer (Agilent, Palo Alto, CA). more...

Organism:

Homo sapiens

Type:

Expression profiling by array

Dataset:

GDS1064

Platform:

GPL96

43 Samples

Download data: CEL

Expression profiling of bone marrow from 43 patients with various forms of acute myeloid leukemia (AML): 10 promyelocytic leukemias with t(15;17), 4 AMLs with inv(16), 7 monocytic leukemias, and 22 nonmonocytic leukemias. Results identify expression signatures unique to each AML subclass.

Organism:

Homo sapiens

Type:

Expression profiling by array, count, 8 disease state sets

Platform:

GPL96

Series:

GSE1729

43 Samples

Download data: CEL

(Submitter supplied) Uterine cervix normal SAGE, CGAP non-normalized SAGE library, bulk method , HPV negative

Organism:

Homo sapiens

Type:

Expression profiling by SAGE

Platform:

GPL4

1 Sample

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(Submitter supplied) This SuperSeries is composed of the SubSeries listed below.

Organism:

Homo sapiens

Type:

Expression profiling by array

Platform:

GPL6947

206 Samples

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(Submitter supplied) Background: Gene expression profiling has shown its ability to identify with high accuracy low cytogenetic risk acute myeloid leukemia such as acute promyelocytic leukemia and leukemias with t(8;21) or inv(16). The aim of this gene expression profiling study was to evaluate to what extent suboptimal samples with low leukemic blast load (range, 2-59%) and/or poor quality control criteria could also be correctly identified. more...

Organism:

Homo sapiens

Type:

Expression profiling by array

Platform:

GPL6947

117 Samples

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(Submitter supplied) Background: Gene expression profiling has shown its ability to identify with high accuracy low cytogenetic risk acute myeloid leukemia such as acute promyelocytic leukemia and leukemias with t(8;21) or inv(16). The aim of this gene expression profiling study was to evaluate to what extent suboptimal samples with low leukemic blast load (range, 2-59%) and/or poor quality control criteria could also be correctly identified. more...

Organism:

Homo sapiens

Type:

Expression profiling by array

Platform:

GPL6947

89 Samples

Download data: TXT

(Submitter supplied) Acute myeloid leukemia (AML) results from aberrant hematopoietic processes and these changes are frequently initiated by chromosomal translocations. One particular subtype, AML with translocation t(7;12)(q36;p13), is found in children diagnosed before two years of age. The mechanisms for leukemogenesis induced by t(7;12) is not understood, in part because of the lack of efficient methods to reconstruct the leukemia-associated genetic aberration with correct genomic architecture and regulatory elements. more...

Organism:

Homo sapiens

Type:

Expression profiling by high throughput sequencing

Platform:

GPL18573

7 Samples

Download data: TXT, XLSX

(Submitter supplied) A transcriptome study in mouse hematopoietic stem cells was performed using a sensitive SAGE method, in an attempt to detect medium and low abundant transcripts expressed in these cells. Among a total of 31,380 unique transcript, 17,326 (55%) known genes were detected, 14,054 (45%) low-copy transcripts that have no matches to currently known genes. 3,899 (23%) were alternatively spliced transcripts of the known genes and 3,754 (22%) represent anti-sense transcripts from known genes.

Organism:

Mus musculus

Type:

Expression profiling by SAGE

Platform:

GPL11

1 Sample

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(Submitter supplied) PURPOSE: To compare the gene expression profiles of normal human corneal endothelium with Fuchs' corneal endothelium, by using serial analysis of gene expression (SAGE). METHODS: Three pairs of normal human corneas were obtained from eye banks. Thirteen bisected Fuchs' corneal buttons were processed at the time of corneal transplantation. The endothelia of normal and Fuchs'-affected corneas were stripped, and total RNA was isolated. more...

Organism:

Homo sapiens

Type:

Expression profiling by SAGE

Platform:

GPL4

2 Samples

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(Submitter supplied) The human leukemia cell line U937 is a well-established model for studying monocytic cell differentiation. We used a modified protocol (SADE) of serial analysis of gene expression (SAGE) and developed a SADE linker-anchored PCR assay to investigate the pattern of expression of known genes and to identify new transcripts in proliferating cells and during cell growth arrest and differentiation. We implemented new informatic tools to compare expression profiles before and after exposure of cells to differentiation inducers. more...

Organism:

Homo sapiens

Type:

Expression profiling by SAGE

Platform:

GPL6

2 Samples

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(Submitter supplied) Microarray gene expression (MAGE) signatures allow insights into the transcriptional processes of leukemias and may evolve as a molecular diagnostic test. Introduction of MAGE into clinical practice of leukemia diagnosis will require comprehensive assessment of variation due to the methodologies. For the first time, we present a study focused on analyzing the impact of different RNA preparation procedures on gene expression data for different subtypes of pediatric acute leukemias. more...

Organism:

Homo sapiens

Type:

Expression profiling by array

Dataset:

GDS2819

Platform:

GPL570

99 Samples

Download data: CEL, CHP

Analysis of RNA samples prepared from leukemic white blood cells using 3 different protocols. Samples obtained from 27 pediatric patients with various subtypes of acute leukemia. Results provide insight into the impact of RNA preparation methods on the variation in gene expression data.

Organism:

Homo sapiens

Type:

Expression profiling by array, count, 9 disease state, 27 individual, 3 protocol sets

Platform:

GPL570

Series:

GSE7757

99 Samples

Download data: CEL, CHP

(Submitter supplied) Temporal dynamics of gene expression are informative of changes associated with disease development and evolution. Given the complexity of high-dimensionaltemporal datasets, an analytical framework guided by a robust theory is needed to interpret time-sequential changes and to predict system dynamics. Herein, we use acute myeloid leukemia as a proof-of-principle to model gene expression dynamics in a transcriptome state-space constructed based on time-sequential RNA-sequencing data. more...

Organism:

Mus musculus

Type:

Expression profiling by high throughput sequencing

Platform:

GPL17021

248 Samples

Download data: TSV, TXT

(Submitter supplied) Gene expression profiling was carried out on peripheral blood mononuclear cell mRNA samples collected from 93 older adults participating in the Chicago Health Aging and Social Relations Study (CHASRS). The primary research question is whether gene expression differs in participants who are chronically lonely. Keywords: Risk prediction

Organism:

Homo sapiens

Type:

Expression profiling by array

Dataset:

GDS3898

Platform:

GPL6883

93 Samples

Download data: TXT

Analysis of PBMCs from chronically lonely, older adults. Lack of close social ties is a well-established risk factor for diseases involving the immune system and inflammation. Results provide insight into the molecular mechanisms underlying those effects.

Organism:

Homo sapiens

Type:

Expression profiling by array, count, 2 individual sets

Platform:

GPL6883

Series:

GSE25837

93 Samples

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