GEO DataSets

(Submitter supplied) In order to identify novel gene targets of vasopressin regulation in the renal medulla, we performed a cDNA microarray study on the inner medullary tissue of mice following a 48 hour water restriction protocol. Keywords: Stress Response

Organism:

Mus musculus

Type:

Expression profiling by array

Platform:

GPL3004

12 Samples

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(Submitter supplied) Identification of gene expressed in the enriched inner medullary collecting duct cells in rat. Keywords: gene expression comparision between cell type

Organism:

Rattus norvegicus

Type:

Expression profiling by array

Dataset:

GDS3150

Platform:

GPL1355

6 Samples

Download data: CEL, CHP

Analysis of inner medullary collecting duct (IMCD) cells isolated from the renal inner medulla. The IMCD is the terminal portion of the kidney's collecting duct system. Results provide insight into molecular mechanisms underlying aquaporin-2 regulation and osmotic water permeability in the IMCD.

Organism:

Rattus norvegicus

Type:

Expression profiling by array, count, 2 cell type sets

Platform:

GPL1355

Series:

GSE7891

6 Samples

Download data: CEL, CHP

(Submitter supplied) Vasopressin is the major hormone that regulates renal water excretion. It does so by binding to a receptor in renal collecting duct cells, triggering signaling pathways that ultimately regulate the abundance, location, and activity of the water channel protein aquaporin 2. We took an advantage of quantitative large scale proteomic technologies and oligonucleotide microarrays to quantify steady state changes in protein and transcript abundances in response to vasopressin in a collecting duct cell line, mpkCCD clone 11 (Yu et al. more...

Organism:

Mus musculus

Type:

Expression profiling by array

Platform:

GPL1261

6 Samples

Download data: CEL, CHP

(Submitter supplied) Notch signaling deficient kidney collecting ducts may serve as a useful resource to identify principal cell lineage and intercalated lineage specific factors since they develop a reduced number of principal cells and an increased number of intercalated cells compared with wild type kidney collecting ducts. We compared RNA from three E18.5 mouse kidneys per group: HoxB7Cre;RBPJf/-;Rosa+/Eyfp (Notch signaling deficient; mutant) versus RBPJf/f;Rosa+/Eyfp (wild type).

Organism:

Mus musculus

Type:

Expression profiling by array

Platform:

GPL17543

6 Samples

Download data: IDAT, TXT

(Submitter supplied) In this study, we analyzed the transcriptome of a highly differentiated mouse clonal CCD principal cell line (mpkCCD(cl4)) and the changes in the transcriptome induced by aldosterone and vasopressin. Serial analysis of gene expression (SAGE) was performed on untreated cells and on cells treated with either aldosterone or vasopressin for 4 h. The transcriptomes in these three experimental conditions were determined by sequencing 169,721 transcript tags from the corresponding SAGE libraries. more...

Organism:

Mus musculus

Type:

Expression profiling by SAGE

Platform:

GPL275

3 Samples

Download data

(Submitter supplied) A series contains a set of transcript intensity values measured by Affymetrix microarray. This SuperSeries is composed of the SubSeries listed below.

Organism:

Mus musculus; Rattus norvegicus

Type:

Expression profiling by array

Platforms:

GPL1261 GPL1355

12 Samples

Download data: CEL, CHP

(Submitter supplied) Freshly isolated rat kidney medullary thick ascending limbs were subjected for transcript profiling.

Organism:

Rattus norvegicus

Type:

Expression profiling by array

Platform:

GPL1355

3 Samples

Download data: CEL, CHP

(Submitter supplied) Freshly isolated rat kidney proximal tubules were subjected for transcript profiling.

Organism:

Rattus norvegicus

Type:

Expression profiling by array

Platform:

GPL1355

3 Samples

Download data: CEL, CHP

(Submitter supplied) This series of microarray data contain transcript intensity of mpkCCD cells.

Organism:

Mus musculus

Type:

Expression profiling by array

Platform:

GPL1261

6 Samples

Download data: CEL, CHP

(Submitter supplied) Vasopressin, a peptide hormone, controls renal water excretion, largely through regulation of water channel aquaporin-2 (AQP2) in the renal collecting duct. There are two regulatory mechanisms of AQP2: 1) short-term regulation by membrane trafficking of AQP2; and 2) long-term regulation involving vasopressin-induced changes of protein abundance of AQP2 through regulation of gene transcription and protein half-life. more...

Organism:

Mus musculus

Type:

Genome binding/occupancy profiling by high throughput sequencing

Platform:

GPL21493

4 Samples

Download data: BED, BW

(Submitter supplied) Vasopressin, a peptide hormone, controls renal water excretion, largely through regulation of water channel aquaporin-2 (AQP2) in the renal collecting duct. There are two regulatory mechanisms of AQP2: 1) short-term regulation by membrane trafficking of AQP2; and 2) long-term regulation involving vasopressin-induced changes of protein abundance of AQP2 through regulation of gene transcription and protein half-life. more...

Organism:

Mus musculus

Type:

Genome binding/occupancy profiling by high throughput sequencing

Platform:

GPL13112

22 Samples

Download data: BED, BEDGRAPH

(Submitter supplied) Purpose: The goal of this study is to identify vasopressin-regulated genes in mouse kidney collecting duct cell line mpkCCD. To explore dynamic regulation of vasoressin-mediated transcriptional regulation, transcriptome of vasopressin-treated cells at four different time points (3h, 6h, 12h, and 24h) were profiled and analyzed. Methods: Total RNAs were isolated from vasopressin-treated mpkCCD cells at different time points (3h, 6h, 12h, and 24h). more...

Organism:

Mus musculus

Type:

Expression profiling by high throughput sequencing

Platform:

GPL21493

24 Samples

Download data: TXT

(Submitter supplied) To investigate the role of miRNAs in the principal cell of kidney collecting duct, a Cre-LoxP recombination strategy was used to generate a mouse model lacking the endoribonuclease Dicer in AQP2 expressing cells (DicerAQP2Cre+). To identify miRNAs altered in DicerAQP2Cre+ mice, small RNA-sequencing analysis was performed on inner medulla tissue from 1-month-old DicerAQP2Cre+ mice and controls (n=3+3 mice). more...

Organism:

Mus musculus

Type:

Non-coding RNA profiling by high throughput sequencing

Platform:

GPL17021

6 Samples

Download data: TXT

(Submitter supplied) We would like to know the gene expression pattern in absence of transcription factor GATA2 in adult renal collecting duct We used Gata2 flox::Pax8-rtTA::Tet-Cre to make a doxycycline induced Gata2 renal tubule cell specific knockout mice

Organism:

Mus musculus

Type:

Expression profiling by array

Platform:

GPL4134

2 Samples

Download data: TXT, XLSX

(Submitter supplied) Water permeability of the kidney collecting ducts is regulated in part by the amount of the molecular water channel protein aquaporin-2 (AQP2), whose expression, in turn, is regulated by the pituitary peptide hormone vasopressin. We previously showed that stable glucocorticoid receptor knockdown diminished the vasopressin-induced Aqp2 gene expression in the collecting duct cell model mpkCCD. Here, we investigated the pathways regulated by the glucocorticoid receptor by comparing transcriptomes of the mpkCCD cells with or without stable glucocorticoid receptor knockdown. more...

Organism:

Mus musculus

Type:

Expression profiling by high throughput sequencing

Platform:

GPL24247

3 Samples

Download data: XLSX

(Submitter supplied) RNA-seq technology was used to reveal the transcriptome changes of tubular epithelia in response to fluid flow and determine the role of primary cilia in this process. Many fluid flow-sensitive genes were identified, among which are those regulated by primary cilia sensing of fluid flow. These genes were further validated by RT-qPCR.

Organism:

Mus musculus

Type:

Expression profiling by high throughput sequencing

Platform:

GPL19057

18 Samples

Download data: TXT

(Submitter supplied) In these studies we have for the first time described the transcriptome of the rat SFO, and have in addition identified genes the expression of which is significantly modified by either water or food deprivation.

Organism:

Rattus norvegicus

Type:

Expression profiling by array

Platform:

GPL1355

15 Samples

Download data: CEL, CHP

(Submitter supplied) Hypertonicity in renal medulla is essential for urine concentration and water homeostasis. However, how renal medullary collecting duct cells (MCDs) survive and function under the harsh hypertonic stress remains incompletely understood. By using the RNA-seq technique, we identified SNAT2 (slc38a2) as a novel osmoresponsive neutral amino acid transporter in MCD cells. We found that hypertonic stress induced cell death of MCDs mainly via ferroptosis.

Organism:

Mus musculus

Type:

Expression profiling by high throughput sequencing

Platform:

GPL24247

8 Samples

Download data: TXT

(Submitter supplied) BACKGROUND: The transcript levels of many genes exhibit significant variation in tissue samples from inbred laboratory mice. A microarray experiment was designed to separate transcript abundance variation across samples from adipose, heart, kidney, and liver tissues of C57BL/6J mice into within-mouse and between-mouse components. Within-mouse variance captures variation due to heterogeneity of gene expression within tissues, RNA-extraction, and array processing. more...

Organism:

Mus musculus

Type:

Expression profiling by array

Platforms:

GPL6481 GPL6246

120 Samples

Download data: CEL, TXT