GEO DataSets

(Submitter supplied) We evaluated the possible mechanisms by which 2-methoxyacetic acid (MAA) disrupts spermatogenesis using high density microarrays. Levels of mRNA transcripts were determined in total RNA isolated from testes of MAA-treated (650 mg/kg i.p.) or concurrent control rats sacrificed 4, 8, 12 or 24 hrs post exposure (PE). Keywords: Toxicology, Time Course,

Organism:

Rattus norvegicus

Type:

Expression profiling by array

Dataset:

GDS3184

Platform:

GPL890

47 Samples

Download data: TXT

Analysis of testes of males exposed to methoxyacetic acid (MAA) for up to 24 hours. MAA is an in vivo metabolite of ethylene glycol monomethoxy ether, a solvent in industrial and household applications. Results provide insight into the mechanisms underlying the disruption of spermatogenesis by MAA.

Organism:

Rattus norvegicus

Type:

Expression profiling by array, log2 ratio, 2 agent, 4 time sets

Platform:

GPL890

Series:

GSE10032

47 Samples

Download data: TXT

(Submitter supplied) This SuperSeries is composed of the SubSeries listed below.

Organism:

Mus musculus

Type:

Expression profiling by high throughput sequencing; Genome binding/occupancy profiling by high throughput sequencing

Platform:

GPL21273

36 Samples

Download data: BW

(Submitter supplied) This study aims to explore the transcriptomic changes in Stra8-cre/Hdac3fl/- mice at meiotic and postmeiotic stages. Pachytene spermatocytes and round spermatids were isolated from testes of Hdac3fl/+ and Stra8-cre/Hdac3fl/- mice at 7-8 week-old through STA-PUT method. Cells from 6 WT mice or 10 Stra8-cre/Hdac3fl/- mice were pooled for one biological replicate. RNA-seq libraries were prepared in biological triplicates.

Organism:

Mus musculus

Type:

Expression profiling by high throughput sequencing

Platform:

GPL21273

12 Samples

Download data: XLSX

(Submitter supplied) We reported changes in the recruitment of H3K9ac and H3K27ac in HDAC3-depleted mouse testes in the late meiotic and early haploid stages. We also investigated genome-wide occupancy of HDAC3, NCOR and Sox30 to the mouse testes from wild-type and Sox30 KO mice.

Organism:

Mus musculus

Type:

Genome binding/occupancy profiling by high throughput sequencing

Platform:

GPL21273

24 Samples

Download data: BW

(Submitter supplied) To determine the changes in gene expression of testes from postnatal day 6 pups, a window in development where spermatogonial stem cells is predominant, following paternal exposure to saline or BEP, a chemotherapeutic coktail used to treat testicular cancer

Organism:

Rattus norvegicus

Type:

Expression profiling by array

Platform:

GPL15084

10 Samples

Download data: TXT

(Submitter supplied) Signal intensity data for rpd3 delete, H3delta(1-28), H3(K4,9,14,18,23,27Q), H4delta(2-26), H4(K5,8,12,16Q), rpd3 delete H3delta(1-28), and rpd3 delete H4(K5,8,12,16Q) yeast grown in rich (YPD) media Keywords = histones Keywords = chromatin Keywords = rpd3 Keywords = HDAC Keywords = histone deacetylase Keywords = yeast Keywords: other

Organism:

Saccharomyces cerevisiae

Type:

Expression profiling by array

Dataset:

GDS772

Platform:

GPL90

18 Samples

Download data: CEL, EXP

Analysis of gene regulation by expression profiling of single mutants for histone deacetylase RPD3, histones H3 and H4, and double mutants for either RPD3 and H3 or RPD3 and H4. Histone mutants defective in amino terminal tails, which are acetylated and deacetylated in transcription regulation.

Organism:

Saccharomyces cerevisiae

Type:

Expression profiling by array, count, 8 genotype/variation sets

Platform:

GPL90

Series:

GSE1639

18 Samples

Download data: CEL, EXP

(Submitter supplied) Methoxyacetic acid (MAA) is the active metabolite of the widely used industrial chemical ethylene glycol monomethyl ether, an established testicular toxicant. MAA induces the degradation of testicular germ cells in association with changes in gene expression in both germ cells and Sertoli cells of the testis. This study investigates the impact of MAA on gene expression in testicular Leydig cells, which play a critical role in germ cell survival and male reproductive function. more...

Organism:

Mus musculus

Type:

Expression profiling by array

Platform:

GPL4134

12 Samples

Download data: TXT

(Submitter supplied) We used H3f3b-/- testes to analyze the impact of H3f3b derived H3.3 on spermatogenesis.

Organism:

Mus musculus

Type:

Genome binding/occupancy profiling by high throughput sequencing

Platform:

GPL13112

8 Samples

Download data: BED

(Submitter supplied) Total gene expression analysis was performed on RNA from testes extracted from two litters of constitutive homozygous and heterozygous H3f3b knockout mice compared to WT littermates.

Organism:

Mus musculus

Type:

Expression profiling by array

Platform:

GPL6887

6 Samples

Download data: TXT

(Submitter supplied) Optimal androgen signaling is critical for testicular development and spermatogenesis. Methoxyacetic acid (MAA), the primary active metabolite of the industrial chemical ethylene glycol monomethyl ether, disrupts spermatogenesis and causes testicular atrophy. Transcriptional trans-activation studies have indicated that MAA can enhance androgen receptor activity, however, whether MAA actually impacts the expression of androgen-responsive genes in vivo, and which genes might be affected is not known. more...

Organism:

Mus musculus

Type:

Expression profiling by array

Platform:

GPL4134

8 Samples

Download data: TXT