GEO DataSets

(Submitter supplied) We determined dynamic behavior of S. agalactiae transcriptome during growth in THY medium and detected growth phase regulated genes

Organism:

Streptococcus agalactiae

Type:

Expression profiling by array

Platform:

GPL6923

12 Samples

Download data: CEL

(Submitter supplied) We have discovered that GBS significantly remodels its transcriptome in response to exposure to human amniotic fluid. A large number of the affected genes are of unknown function, which means that much remains to be learned about the full influence of amniotic fluid on GBS. The majority of the observed changes in transcripts affects genes involved in basic bacterial metabolism and is connected to AF composition and nutritional requirements of the bacterium. more...

Organism:

Streptococcus agalactiae

Type:

Expression profiling by array

Platform:

GPL6923

9 Samples

Download data: CEL, CHP

(Submitter supplied) Background. For more than 100 years, group A Streptococcus has been identified as a cause of severe, and in many cases fatal, infections of the female urogenital tract. Due to advances in hospital hygiene and the advent of antibiotics, this type of infection has been virtually eradicated. However, within the last three decades there has been an increase in severe intra- and post-partum infections attributed to GAS. more...

Organism:

Streptococcus pyogenes MGAS6180

Type:

Expression profiling by array

Platform:

GPL9953

18 Samples

Download data: CEL

(Submitter supplied) In the present investigation, we conducted a bacterial transcriptome dynamics analysis during GBS incubation with human blood. We observed a large modification in the expression of genes involved in transcriptional regulation and in the metabolism of carbohydrates, purins/pyrimidins and inorganic ions, and an up-regulation of genes involved in attachment and plasminogen contact. We also observed differences in the expression of GBS genes in blood at 40° relative to 37°C.

Organism:

Streptococcus agalactiae

Type:

Expression profiling by array

Platform:

GPL6923

34 Samples

Download data: CEL

(Submitter supplied) To understand the extent to which GBS modify gene expression in response to temperatures encountered in the various hosts, we conducted a whole genome transcriptome analysis of organisms grown at 30°C and 40°C. We identified extensive transcriptome remodeling at various stages of growth, especially in the stationary phase (significant transcript changes occurred for 25% of the genes). A large proportion of genes involved in metabolism was up-regulated at 30°C in stationary phase, which reflects a slowing of bacterial metabolism in the early stages of its shift to growth at lower temperature followed by an acceleration in the later stages. more...

Organism:

Streptococcus agalactiae

Type:

Expression profiling by array

Platform:

GPL6923

18 Samples

Download data: CEL

(Submitter supplied) We have conducted RNA-seq analysis on Group B Streptococcus (GBS) when interacting with human stem-cell (hSC) derived BECs. The study contains a control group for GBS and a control group for BECs each with three biological replicates. The last group contains GBS infection BECs and also has three replicates.

Organism:

Homo sapiens; Streptococcus sp. 'group B'

Type:

Expression profiling by high throughput sequencing

Platforms:

GPL31999 GPL18573 GPL31998

9 Samples

Download data: CSV

(Submitter supplied) In the present investigation, we conducted a bacterial transcriptome dynamics analysis during GBS incubation with human blood. We observed large modification in the expression of numerous genes, but GBS transcriptome was different with the blood of one donor. More genes were up-regulated and less down-regulated in respect to the other donors.

Organism:

Streptococcus agalactiae

Type:

Expression profiling by array

Platform:

GPL6923

5 Samples

Download data: CEL

(Submitter supplied) The purpose of this study was to determine what S. agalactiae genes are under the control of the MtaR regulatory protein. Inactivation of the Streptococcus agalactiae mtaR gene resulted in the downregulation of 11 genes and the upregulation of 1 gene in the mtaR mutant. Genes involved in the uptake of methionine and a gene involved in the degradation of peptides were downregulated. Also, genes involved in the transport and biosynthesis of arginine were downregulated. more...

Organism:

Streptococcus agalactiae

Type:

Expression profiling by array

Platform:

GPL7515

6 Samples

Download data: CEL, CHP

(Submitter supplied) Transcriptome analysis of Streptococcus agalactiae (group B Streptococcus) grown under control conditions or coincubated with serine hydroxamate to induce the bacterial stringent response

Organism:

Streptococcus agalactiae

Type:

Expression profiling by high throughput sequencing

Platform:

GPL23396

12 Samples

Download data: TXT

(Submitter supplied) As part of a broader study to identify genes that contribute to fitness of the human pathobiont Streptococcus agalacitae (group B Streptococcus), we identified a GntR-class transcription factor, named mrvR, which contributes to bacterial persistence in human amniotic fluid and multipe virulence phenotypes. In order to understand the transcriptome of mrvR, whole-genome transcriptomic analysis was performed with wild type group B Streptococcus and an mrvR deletion mutant at three growth phases.

Organism:

Streptococcus agalactiae

Type:

Expression profiling by high throughput sequencing

Platform:

GPL29157

12 Samples

Download data: XLSX

(Submitter supplied) Early events leading to intrauterine infection remain poorly defined, but may hold the key to a therapeutic intervention for preterm delivery. To determine early molecular pathways associated with membrane weakening that may progress to preterm premature rupture of membranes (PPROM), we examined the effects of Group B Streptococcus (GBS) on fetal membranes after a choriodecidual infection.

Organism:

Macaca mulatta; Macaca nemestrina

Type:

Expression profiling by array

Platform:

GPL3535

10 Samples

Download data: CEL

(Submitter supplied) Total RNA was isolated from mid-log phase Streptococcus agalactiae 874391 wild-type cells grown in Todd-Hewitt broth (THB) medium and sequenced using Illumina NextSeq500

Organism:

Streptococcus agalactiae

Type:

Expression profiling by high throughput sequencing

Platform:

GPL29157

4 Samples

Download data: XLSX

(Submitter supplied) Total RNA was isolated from mid-log phase Streptococcus agalactiae 874391 wild-type cells grown in Todd-Hewitt broth (THB) medium supplemented with 0.25 mM Zn and sequenced using Illumina NextSeq500.

Organism:

Streptococcus agalactiae

Type:

Expression profiling by high throughput sequencing

Platform:

GPL29157

4 Samples

Download data: XLSX

(Submitter supplied) Sa strain 874391 and human U937 monocytes at 6h post-infection

Organism:

Streptococcus agalactiae; Homo sapiens

Type:

Expression profiling by high throughput sequencing

Platforms:

GPL29157 GPL18573 GPL29372

12 Samples

Download data: XLSX

(Submitter supplied) Genes that showed altered expression between wild type A909 and isogenic rgfC/A mutant were identified. A strain with a deletion in RgfC/A (SAK_1917/SAK_1918) was derived and microarray analysis was performed to identify genes regulated by the RgfC/A two component system. strain comparison, global regulation, two component system

Organism:

Streptococcus agalactiae A909; Streptococcus agalactiae

Type:

Expression profiling by array

Platform:

GPL10363

2 Samples

Download data: TXT

(Submitter supplied) Intrauterine exposure to amniotic fluid (AF) cytokines is thought to predispose to bronchopulmonary dysplasia (BPD). We evaluated the effects of GBS exposure on RNA expression in fetal lung tissue to determine early molecular pathways associated with fetal lung injury that may progress to BPD.

Organism:

Macaca mulatta; Macaca nemestrina

Type:

Expression profiling by array

Platform:

GPL3535

10 Samples

Download data: CEL

(Submitter supplied) Global regulatory roles of the TCS response regulator CovR in GBS serotype 1a A909 Keywords: Strain comparison; global regulation

Organism:

Streptococcus agalactiae A909; Streptococcus agalactiae

Type:

Expression profiling by array

Platform:

GPL6019

2 Samples

Download data: TXT

(Submitter supplied) We conducted genome-wide microarray analyses to determine the regulons of RcsB and RcsC in liquid medium and, for the first time, on immature pear fruit. Our array analyses identified a total of 648 genes differentially regulated by the RcsCB in vitro and in vivo. Consistent with our previous findings, RcsB acts as a positive regulator in both conditions, while RcsC positively controls amylovoran biosynthetic gene expression in vivo, but negatively in vitro. more...

Organism:

Erwinia amylovora

Type:

Expression profiling by array

Platform:

GPL13939

12 Samples

Download data: GPR

(Submitter supplied) Streptococcus agalactiae (Group B Streptococcus, GBS) can colonize the human vaginal tract leading to both superficial and serious infections in adults and neonates. To study bacterial colonization of the reproductive tract in a mammalian system, we employed a murine vaginal carriage model. Using RNASeq, the transcriptome of GBS growing in vivo during vaginal carriage was determined. Over one-quarter of the genes in GBS were found to be differentially regulated during in vivo colonization as compared to laboratory cultures. more...

Organism:

Streptococcus agalactiae A909

Type:

Expression profiling by high throughput sequencing

Platform:

GPL24543

14 Samples

Download data: TXT

(Submitter supplied) Amniotic fluid (AF) is a complex biological material that provides a unique window into the developing human. Residual AF supernatant contains cell-free fetal RNA. The objective of this study was to develop an understanding of the AF core transcriptome by identifying the transcripts ubiquitously present in the AF supernatant of euploid midtrimester fetuses. We detected 476 well-annotated genes present in all twelve AF samples. more...

Organism:

Homo sapiens

Type:

Expression profiling by array

Platform:

GPL570

12 Samples

Download data: CEL