GEO DataSets

(Submitter supplied) This study was conducted to examine normal gene expression in the heart during mouse embryonic development.

Organism:

Mus musculus

Type:

Expression profiling by array

Platform:

GPL339

5 Samples

Download data: CEL, CHP

(Submitter supplied) The transcription factor Nkx2.5 is required for specification of pharyngeal arch second heart field (SHF) progenitors that contribute to outflow tract (OFT) and right ventricle (RV) formation. Multiple sets of microarray data were analyzed to identify genes that are candidate targets of Nkx2.5 in the second heart field. These sets are: 1) publicly available data for cardiothoracic tissue from E9.5 Nkx2.5 wild-type, heterozygous and homozygous embryos; 2) an analysis of mouse E10.5 pharyngeal arch tissue; 3) an analysis of mouse E12.5 heart tissue; and 4) a temporal analysis of the cardiogenic cell line P19CL6. more...

Organism:

Mus musculus

Type:

Expression profiling by array

Datasets:

GDS3802 GDS3803

Platforms:

GPL1261 GPL339

26 Samples

Download data: CEL, CHP

(Submitter supplied) This study was conducted to examine normal gene expression in the pharyngeal arch during mouse embryonic development

Organism:

Mus musculus

Type:

Expression profiling by array

Platform:

GPL339

5 Samples

Download data: CEL, CHP

(Submitter supplied) Pluripotent P19CL6 embryonic carcinoma cells can be differentiated to a cardiac lineage by culture in the presence of DMSO. The goal of this study was to characterize temporal gene expression patterns associated with cardiogenic differentiation. Gene expression analysis was conducted on differentiating P19CL6 cells at several time points following induction with 1% DMSO. Samples were processed for analysis by Affymetrix GeneChip.

Organism:

Mus musculus

Type:

Expression profiling by array

Platform:

GPL1261

16 Samples

Download data: CEL, CHP

Analysis of second heart field (SHF)-containing pharyngeal arch from E10.5 embryos and heart from E12.5 embryos. These results, together with results from P19CL6 cells induced to differentiate into a cardiac lineage, provide insight into Nkx2.5 target genes relevant specifically to the SHF.

Organism:

Mus musculus

Type:

Expression profiling by array, count, 2 age, 2 tissue sets

Platform:

GPL339

Series:

GSE17936

10 Samples

Download data: CEL, CHP

Temporal analysis of P19CL6 cells induced to differentiate into a cardiac lineage in the presence of DMSO. These results, together with results from E10.5 second heart field (SHF)-containing pharyngeal arch and E12.5 heart, provide insight into Nkx2.5 target genes relevant specifically to SHF.

Organism:

Mus musculus

Type:

Expression profiling by array, transformed count, 2 growth protocol, 6 time sets

Platform:

GPL1261

Series:

GSE17936

16 Samples

Download data: CEL, CHP

(Submitter supplied) To identify a functional gene regulatory network that orchestrate transcriptional programs to direct proper outflow tract development, we generated transgenic mice harboring a 226bp-deletion within a GATA-dependent, highly conserved cardiac enhancer region. We then performed gene expression profiling analysis using data obtained from RNA-seq of outflow tract in wildtype and homozygous mutant littermates at embryonic day 12.5.

Organism:

Mus musculus

Type:

Expression profiling by high throughput sequencing

Platform:

GPL24247

9 Samples

Download data: TXT

(Submitter supplied) Jarid2 cooperates with PRC2 for H3K27me3 accumulation on a subset of Jarid2 target genes in the developing heart, which contributes to repress differentiation of other lineages such as neural differentiation, and to guide normal myocardial development. Jarid2 forms a functional complex with PRC2 to increase or maintain H3K27me3 levels on the specific promoters in the developing heart.

Organism:

Mus musculus

Type:

Genome binding/occupancy profiling by genome tiling array

Platform:

GPL16605

3 Samples

Download data: FTR, TXT

(Submitter supplied) Development of the vertebrate heart requires the appropriate integration of temporally-distinct differentiating progenitor populations. While factors that promote accrual of later-differentiating second heart field (SHF)-derived cells to the outflow tract (OFT) have been intensively investigated, few signals are understood that specifically restrict the size of the vertebrate OFT. Here, we show that improper specification and proliferation of SHF progenitors in zebrafish lazarus (lzr) mutants, which lack the transcription factor Pbx4, produces enlarged hearts with a specific increase in ventricular cardiomyocytes and smooth muscle cells within the OFT. more...

Organism:

Danio rerio

Type:

Expression profiling by high throughput sequencing

Platform:

GPL18413

2 Samples

Download data: MTX, TSV

(Submitter supplied) Analysis of the roles of Irx3 and Irx5 transcription factors in mouse heart development and postnatal heart function. Results show that show that Irx3 and Irx5 have redundant function in the in the endocardium to regulate atrioventricular canal morphogenesis and outflow tract formation. A postnatal deletion of Irx3 and Irx5 surprisingly results in a restoration of the repolarization gradient that is altered in Irx5 mutant hearts, suggesting a model whereby postnatal Irx3 activity is normally repressed by Irx5.

Organism:

Mus musculus

Type:

Expression profiling by array

Dataset:

GDS4317

Platform:

GPL6246

14 Samples

Download data: CEL, TXT

Analysis of E12.5 hearts deficient in both of the Iroquois homeodomain transcription factors, Irx3 and Irx5. Results provide insight into the molecular mechanisms underlying cooperative and antagonistic roles for Irx3 and Irx5 in cardiac morphogenesis.

Organism:

Mus musculus

Type:

Expression profiling by array, transformed count, 4 genotype/variation sets

Platform:

GPL6246

Series:

GSE38826

14 Samples

Download data: CEL, TXT

(Submitter supplied) NKX2-5 is a homeodomain transcription factor that plays a central role in the cardiac gene regulatory network, and is commonly mutated in human congenital heart disease. Here, we take a functional genomics approach to congenital heart disease mechanism. We used DamID to establish a robust set of target genes for both wild type NKX2-5 and a mutation lacking the homeodomain (NKX2-5delHD), the latter to model loss-of-function in gene regulatory network. more...

Organism:

Mus musculus

Type:

Genome binding/occupancy profiling by genome tiling array

Platform:

GPL5811

10 Samples

Download data: BED, CEL, COD