GEO DataSets

(Submitter supplied) The callipyge phenotype is a monogenic muscular hypertrophy that is only expressed in heterozygous sheep receiving the CLPG mutation from their sire. The wild-type phenotype of CLPG/CLPG animals is thought to result from translational inhibition of paternally expressed DLK1 transcripts by maternally expressed miRNAs. To identify the miRNA responsible for this trans-effect, we used high-throughput sequencing to exhaustively catalogue miRNAs expressed in skeletal muscle of sheep of the four CLPG genotypes. more...

Organism:

Homo sapiens; Human betaherpesvirus 5; Betapolyomavirus hominis; Mus musculus; Human alphaherpesvirus 1; human gammaherpesvirus 4; Human gammaherpesvirus 8; Betapolyomavirus macacae; Ovis aries; Rattus norvegicus; JC polyomavirus; Human immunodeficiency virus 1

Type:

Non-coding RNA profiling by array

Platform:

GPL7724

8 Samples

Download data: TXT

(Submitter supplied) The callipyge mutation causes postnatal muscle hypertrophy in heterozygous lambs that inherit a paternal callipyge allele (+/CLPG). Our hypothesis was that the up-regulation of one or both of the affected paternally expressed genes (DLK1 or PEG11) initiates changes in biochemical and physiological pathways in skeletal muscle to induce hypertrophy. The goal of this study was to identify changes in gene expression during the onset of muscle hypertrophy in order to identify the pathways that are involved in the expression of the callipyge phenotype. more...

Organism:

Bos taurus; Ovis aries

Type:

Expression profiling by array

Platform:

GPL2112

12 Samples

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(Submitter supplied) Lambs that inherit a callipyge allele from their dam have an up-regulation of maternally imprinted transcripts near the callipyge mutation but do not exhibit muscle hypertrophy. It is not clear what effects these maternally expressed transcripts have in the muscle or how the inheritance of a maternal callipyge allele prevents the expression of the callipyge phenotype which is seen paternal heterozygotes only. more...

Organism:

Ovis aries; Bos taurus

Type:

Expression profiling by array

Platform:

GPL2112

44 Samples

Download data: CEL

(Submitter supplied) Microarrays were used for transcription profiling of skeletal muscle samples taken at birth, when the phenotype was not expressed, and 12 weeks of age from Callipyge and wild type sheep. The genes that underlie the expression of the phenotype rather than result from the fibre type change in the affected muscle have been identified. We used microarrays to detail the global programme of gene expression underlying the hypertrophy phenotype and identified distinct classes of regulated genes during this process. more...

Organism:

Ovis aries; Bos taurus

Type:

Expression profiling by array

Platform:

GPL2112

24 Samples

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(Submitter supplied) The ovine Callipyge mutation causes postnatal muscle hypertrophy localized to the pelvic limbs and torso, as well as body leanness. The mechanism underpinning enhanced muscle mass is unclear, as is the systemic impact of the mutation. Using muscle fibre typing immunohistochemistry we confirmed muscle specific effects and demonstrated that affected muscles had greater prevalence and hypertrophy of type 2X fast twitch glycolytic fibres and decreased representation of types 1, 2C, 2A and/or 2AX fibres. more...

Organism:

Bos taurus; Ovis aries

Type:

Expression profiling by array

Platform:

GPL2112

8 Samples

Download data: CEL

(Submitter supplied) microRNAs (miRNAs), a class of small non-coding RNAs, are key regulators of gene expression at post-transcriptional level and play essential roles in fundamental biological processes such as development and metabolism. Here, we perform a comprehensive analysis of miRNAs in the zoonotic parasite E. canadensis G7, one of the causative agents of the neglected disease cystic echinococcosis. Small RNA libraries from protoscoleces and cyst walls of E. more...

Organism:

Echinococcus canadensis; Echinococcus granulosus

Type:

Non-coding RNA profiling by high throughput sequencing

Platforms:

GPL18185 GPL19605

6 Samples

Download data: TXT

(Submitter supplied) The complex of NF90 and NF45 is known to participate in transcriptional regulation, mRNA stabilization and microRNA biogenesis in vitro. However, the physiological function of the NF90-NF45 complex is still unclear. To elucidate its functions, we generated NF90-NF45 double transgenic (dbTg) mice. Robust expression of NF90 and NF45 was detected in skeletal muscle. As mentioned above, NF90-NF45 complex is involved in regulation of genes via transcription and RNA metabolism. more...

Organism:

Mus musculus

Type:

Expression profiling by array

Platform:

GPL17400

4 Samples

Download data: CEL, CHP

(Submitter supplied) To investigate regulation of miRNA biogenesis by NF90-NF45 complex, we performed comprehensive analysis of miRNA expression in quandriceps of WT and NF90-NF45 dbTg mice.

Organism:

Mus musculus

Type:

Non-coding RNA profiling by array

Platform:

GPL16759

2 Samples

Download data: TXT

(Submitter supplied) The use of assisted reproductive technologies (ART) can induce a congenital overgrowth condition in humans and ruminants, namely Beckwith-Wiedemann syndrome (BWS) and large offspring syndrome (LOS), respectively. Shared phenotypes and epigenotypes have been found between BWS and LOS. We have observed global misregulation of transcripts in bovine foetuses with LOS. microRNAs (miRNAs) are important post-transcriptional gene expression regulators. more...

Organism:

Bos indicus x Bos taurus

Type:

Non-coding RNA profiling by high throughput sequencing

Platform:

GPL25325

45 Samples

Download data: TXT

(Submitter supplied) MicroRNAs (miRNA) are short single-stranded RNA molecules that regulate gene expression post-transcriptionally by binding to complementary sequences in the 3' untranslated region (3' UTR) of target mRNAs. MiRNAs participate in the regulation of myogenesis, and identification of the complete set of miRNAs expressed in muscles is likely to significantly increase our understanding of muscle growth and development. more...

Organism:

Sus scrofa

Type:

Non-coding RNA profiling by high throughput sequencing

Platform:

GPL9126

7 Samples

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(Submitter supplied) Purpose: MicroRNAs (miRNAs) are non-coding small miRNA ~22 nucleotides in length and play a vital role in muscle development by binding to messenger RNAs (mRNAs). Large White (LW, a lean type pig) and Meishan pigs (MS, a Chinese indigenous obese breed) have significant postnatal phenotype differences in growth rate, muscle mass and meat quality, and theses difference are programmed during prenatal muscle development.To shed directly light on the miRNA transcriptome difference in prenatal muscles between these two distinct pig breeds. more...

Organism:

Sus scrofa

Type:

Non-coding RNA profiling by high throughput sequencing

Platform:

GPL11429

6 Samples

Download data: XLS