GEO DataSets

(Submitter supplied) Because refractive development is governed largely by the retina, we analyzed the retinal transcriptome in chicks wearing a spectacle lens, a well-established means to induce refractive errors, to identify gene expression alterations and to develop novel mechanistic hypotheses about refractive development.

Organism:

Gallus gallus

Type:

Expression profiling by array

Dataset:

GDS3924

Platform:

GPL3213

48 Samples

Download data: CEL, CHP

Analysis of retina and retinal pigment epithelium (RPE) from 1 week-old chicks wearing a unilateral spectacle lens of + or 15 diopters for 6 hours or 3 days. The spectacle lens induces refractive errors. Results provide insight into molecular mechanisms underlying refractive development.

Organism:

Gallus gallus

Type:

Expression profiling by array, transformed count, 3 protocol, 2 time sets

Platform:

GPL3213

Series:

GSE24641

48 Samples

Download data: CEL, CHP

(Submitter supplied) The retina plays an important regulatory role in ocular growth. To screen for new retinal candidate genes that could be involved in the inhibition of ocular growth, we used chick microarrays to analyze the changes in retinal mRNA expression after myopic defocus was imposed by positive lens-wear. Chicks were raised under a 12h-light/12h-dark cycle (light-onset: 8:00 am and light-offset: 8:00 pm) with unrestricted access to water and food. more...

Organism:

Gallus gallus

Type:

Expression profiling by array

Platform:

GPL3213

8 Samples

Download data: CEL, EXP

(Submitter supplied) One-day old white Leghorn chicks were housed in brooders with a 12 hr light:dark cycle, using General Electric chroma 50 fluorescent lighting with irradiance of approximately 50μW/cm2 at chick eye level. They received Purina Chick Chow food and water ad libitum. At one week of age and at the onset of the light phase, the chicks were anesthetized with inhalation ether, and a unilateral translucent white plastic goggle was glued to the periorbital feathers to induce ipsilateral form-deprivation myopia, alternating between the left or right eye. more...

Organism:

Gallus gallus

Type:

Expression profiling by array

Platform:

GPL3213

24 Samples

Download data: CEL

(Submitter supplied) Ocular growth is regulated locally by signals produced in the retina that ultimately act on the growth of the scleral tissue. Consequently, a number of studies have investigated changes in retinal gene expression during manipulation of ocular growth in an attempt to elucidate the biochemical pathways underlying eye growth. However, due to the highly heterogenous nature of the retina, important changes in gene expression can be masked. more...

Organism:

Gallus gallus

Type:

Expression profiling by array

Platform:

GPL3213

9 Samples

Download data: CEL

(Submitter supplied) In chicks, the avian homologue of the early growth response protein-1 (ZENK) has been shown to be increased in a special cell type of the retina, the glucagonergic amacrine cells, under conditions that lead to a reduction in eye growth (myopic defocus, recovery of myopia) and decreased under conditions that enhance ocular growth (hyperopic defocus, form-deprivation). The investigation of Egr-1 knock-out mice showed that homozygous knock-out mice with no functional Egr-1 protein developed relative axial myopia at the age of 42 and 56 days, compared to heterozygous- and wildtype Egr-1 knock-out mice. more...

Organism:

Mus musculus

Type:

Expression profiling by array

Dataset:

GDS3607

Platform:

GPL1261

16 Samples

Download data: CEL

Analysis of retinas of Egr-1 deficient animals at post-natal days 30 and 42. 42 day-old Egr-1 knockout animals have longer eyes and a more myopic refractive error compared to wild-types. Results provide insight into the molecular mechanisms regulating axial eye growth.

Organism:

Mus musculus

Type:

Expression profiling by array, count, 2 age, 2 genotype/variation sets

Platform:

GPL1261

Series:

GSE16974

16 Samples

Download data: CEL

(Submitter supplied) Purpose: The morphology of the RPE shows minimal change as the neural retina and choriocapillaris differentiate. Nonetheless, initial studies of barrier-related proteins suggest extensive remodeling of the RPE in response to this changing environment. A genomic approach was used to investigate the extent of this remodeling. Methods: RPE was isolated from E7, E10, E14 and E18 chick embryos and total RNA extracted for probing the entire genome on Affymetrix microarray chips. more...

Organism:

Gallus gallus

Type:

Expression profiling by array

Dataset:

GDS2964

Platform:

GPL3213

14 Samples

Download data: CEL, EXP

Analysis of retinal pigment epithelia (RPE) of E7 to E18 embryos. The RPE regulates transport between the neural retina and fenestrated capillaries of the choroid, a vascular layer between the retina and sclera. Results provide insight into the extent of remodeling of the RPE during development.

Organism:

Gallus gallus

Type:

Expression profiling by array, count, 4 development stage sets

Platform:

GPL3213

Series:

GSE7176

14 Samples

Download data: CEL, EXP

(Submitter supplied) Purpose: The purpose of this study was to develop a framework for analyzing RPE expression profiles from zebrafish eye mutants. Methods: The fish model we used was smarca4 (SWI/SNF related, matrix associated, actin dependent regulator of chromatin, subfamily a, member 4), a retinal dystrophic mutant that the retinal phenotype and expression profiles were previously characterized. Histological and Affymetrix GeneChip analyses were conducted to define the RPE defects and underlying differential expression respectively. more...

Organism:

Danio rerio

Type:

Expression profiling by array

Platform:

GPL1319

18 Samples

Download data: CEL

(Submitter supplied) Eye development and photoreceptor maintenance requires the retinal pigment epithelium (RPE), a thin layer of cells that underlies the neural retina. Despite its importance, RPE development has not been studied by a genomic approach. A microarray expression profiling methodology was established in this study for studying RPE development. The intact retina with RPE attached was dissected from developing embryos, and differentially expressed genes in RPE were inferred by comparing the dissected tissues with retinas without RPE using microarray and statistical analyses. more...

Organism:

Danio rerio

Type:

Expression profiling by array

Dataset:

GDS2556

Platform:

GPL1319

6 Samples

Download data: CEL, DAT

Analysis of microdissected embryonic retinas with or without the retinal pigment epithelium (RPE). The RPE is a thin layer of cells that underlies the neural retina and supports the function and development of photoreceptors. Results provide insight into the development of the RPE.

Organism:

Danio rerio

Type:

Expression profiling by array, count, 2 tissue sets

Platform:

GPL1319

Series:

GSE5048

6 Samples

Download data: CEL, DAT

(Submitter supplied) We investigated diurnal changes in gene expression in the retina and choroid at the onset of experimental myopia. We induced visual form deprivation myopia in young chicks, and isolated retinal and choroidal tissues separately in form- deprived and contralateral control eyes every 4 hours over a single 24-hour period.

Organism:

Gallus gallus

Type:

Expression profiling by high throughput sequencing

Platform:

GPL26853

142 Samples

Download data: TXT