GEO DataSets

(Submitter supplied) Dietary restriction extends lifespan and delays the age-related physiological decline in many species. Intermittent fasting (IF) is one of the most effective dietary restriction regimens that extends lifespan in C. elegans and mammals1,2. In C. elegans, the FOXO transcription factor DAF-16 is implicated in fasting-induced gene expression changes and the longevity response to IF3; however, the mechanisms that sense and transduce fasting-stress stimuli have remained largely unknown. more...

Organism:

Caenorhabditis elegans

Type:

Expression profiling by array

Platform:

GPL200

46 Samples

Download data: CEL

(Submitter supplied) Intermittent fasting is one of the most effective dietary restriction regimens that extend life-span in C. elegans and mammals. Fasting-stimulus responses are key to the longevity response; however, the mechanisms that sense and transduce fasting-stimulus have remained largely unknown. Through a comprehensive transcriptome analysis in C. elegans, we have found that along with the FOXO transcription factor DAF-16, AP-1 (JUN-1/FOS-1) plays a central role in fasting-induced transcriptional changes. more...

Organism:

Caenorhabditis elegans

Type:

Expression profiling by array

Platform:

GPL200

10 Samples

Download data: CEL

(Submitter supplied) Analysis of genes differentially expressed between daf-2(e1368) and daf-2(e1368);pmk-1(km25) and between daf-2(e1368) and daf-2(e1368);daf-16(mgDf47). These studies identified genes upregulated by wild-type PMK-1 and wild-type DAF-16. Keywords: genetic modification

Organism:

Caenorhabditis elegans

Type:

Expression profiling by array

Dataset:

GDS3253

Platform:

GPL200

9 Samples

Download data: CEL

(Submitter supplied) Analysis of differential gene expression in C. elegans adults exposed to three different bacteria: E. coli strain OP50, wild-type P. aeruginosa PA14 and gacA mutant PA14. Samples were analyzed at 4 hours and 8 hours after exposure to the different bacteria. These studies identified C. elegans genes induced by pathogen infection. Keywords: Time course, response to pathogen infection

Organism:

Caenorhabditis elegans

Type:

Expression profiling by array

Dataset:

GDS3252

Platform:

GPL200

18 Samples

Download data: CEL

Analysis of daf-2(e1368) loss-of-function mutant and daf-2(e1368);pmk-1(km25) and daf-2(e1368);daf-16(mgDf47) double mutants. PMK-1 and DAF-16 confer enhanced pathogen resistance on daf-2 mutants relative to wild-type. Results suggest DAF-16 and PMK-1 upregulate distinct genes to promote immunity.

Organism:

Caenorhabditis elegans

Type:

Expression profiling by array, count, 3 genotype/variation sets

Platform:

GPL200

Series:

GSE5801

9 Samples

Download data: CEL

Analysis of strain fer-15(b26);fem-1(hc17) young adults exposed for up to 8 hrs to pathogenic Pseudomonas aeruginosa strain PA14, less pathogenic PA14 mutant gacA, and E. coli strain OP50 (normal food source). Results provide insight into the molecular basis of the response to a bacterial pathogen.

Organism:

Caenorhabditis elegans

Type:

Expression profiling by array, count, 3 infection, 2 time sets

Platform:

GPL200

Series:

GSE5793

18 Samples

Download data: CEL

(Submitter supplied) Dietary restriction regimens lead to enhanced stress resistance and extended lifespan in many species through the regulation of fasting and/or diet responsive mechanisms. The fasting stimulus is perceived by sensory neurons and causes behavioral and metabolic adaptations. Several studies have implicated that the nervous system is involved in the regulation of longevity. However, it remains largely unknown whether the nervous system contributes to the regulation of lifespan and/or stress resistance elicited by fasting. more...

Organism:

Caenorhabditis elegans

Type:

Expression profiling by array

Platform:

GPL200

6 Samples

Download data: CEL

(Submitter supplied) Dietary restriction (DR) is the most effective and reproducible intervention to extend lifespan in divergent species1. In mammals, two regimens of DR, intermittent fasting (IF) and caloric restriction (CR), have proven to extend lifespan and reduce the incidence of age-related disorders2. An important characteristic of IF is that it can increase lifespan, even when there is little or no overall decrease in calorie intake2. more...

Organism:

Caenorhabditis elegans

Type:

Expression profiling by array

Platform:

GPL200

24 Samples

Download data: CEL

(Submitter supplied) Cholesterol has attracted significant attention as a possible lifespan regulator. It has been reported that serum cholesterol levels have an impact on mortality due to age-related disorders such as cardiovascular disease. Diet is also known to be an important lifespan regulator. Dietary restriction retards the onset of age-related diseases and extends lifespan in various organisms. Although cholesterol and dietary restriction are known to be lifespan regulators, it remains to be established whether cholesterol is involved in dietary restriction-induced longevity. more...

Organism:

Caenorhabditis elegans

Type:

Expression profiling by array

Platform:

GPL200

8 Samples

Download data: CEL

(Submitter supplied) In this study we have investigated the effect of loss of math-33 activity on DAF-16-mediated target gene regulation in C. elegans under conditions of reduced Insulin/IGF-1 signaling (IIS). Using whole nematode RNA sequencing experiments we found that the daf-2(e1370)-mediated induction and repression of DAF-16 target genes was decreased in daf-2(e1370); math-33(tm3561) mutant animals. Our data suggest that the downregulation of endogenous DAF-16 isoforms in the absence of a functional MATH-33 severely affects the global expression of DAF-16 targets when IIS activity is reduced. more...

Organism:

Caenorhabditis elegans

Type:

Expression profiling by high throughput sequencing

Platform:

GPL18245

10 Samples

Download data: XLS

(Submitter supplied) Abstract: C. elegans SIR-2.1, a member of the Sir-2 family of NAD(+)-dependent protein deacetylases, has been shown to regulate nematode aging via the insulin/IGF pathway transcription factor daf-16. Treatment of C. elegans with the small molecule resveratrol, however, extends life span in a manner fully dependent upon sir-2.1, but independent of daf-16. Microarray analysis of worms treated with resveratrol demonstrates the transcriptional induction of a family of genes encoding prion-like glutamine/asparagine-rich proteins involved in endoplasmic reticulum (ER) stress response to unfolded proteins. more...

Organism:

Caenorhabditis elegans

Type:

Expression profiling by array

Platforms:

GPL3390 GPL3391

12 Samples

Download data

(Submitter supplied) An all pairs experiment design type is where all labeled extracts are compared to every other labeled extract. Genotype: unc-119+ transgenic worm Keywords: all_pairs

Organism:

Caenorhabditis elegans

Type:

Expression profiling by array

Platform:

GPL3390

4 Samples

Download data

(Submitter supplied) Eggs were isolated by hypochlorite treatment and allowed to hatch in the absence of food as L1 stage arrested worms in S-buffer. Worms were then transferred to large plates containing NGM alone or NGM+ resveratrol and allowed to grow till young adult stage (ca 40 hours). Worms were harvested and total RNA extracted using Trizol reagent. Poly-A+ RNA was isolated using Qiagen midi-prep kit and used for microarray hybridization. more...

Organism:

Caenorhabditis elegans

Type:

Expression profiling by array

Platform:

GPL3391

4 Samples

Download data

(Submitter supplied) Eggs were isolated by hypochlorite treatment and allowed to hatch in the absence of food as L1 stage arrested worms in S-buffer. Worms were then transferred to large plates containing NGM alone or NGM+ resveratrol and allowed to grow till young adult stage (ca 40 hours). Worms were harvested and total RNA extracted using trizol reagent. Poly-A+ RNA was isolated using Qiagen midi-prep kit and used for microarray hybridization. more...

Organism:

Caenorhabditis elegans

Type:

Expression profiling by array

Platform:

GPL3391

4 Samples

Download data

(Submitter supplied) This SuperSeries is composed of the SubSeries listed below.

Organism:

Caenorhabditis elegans; synthetic construct

Type:

Expression profiling by array; Non-coding RNA profiling by array

Platforms:

GPL21572 GPL200

11 Samples

Download data: CEL

(Submitter supplied) Intermittent fasting (IF), a dietary restriction regimen, extends the lifespans of C. elegans and mammals by inducing gene expression changes. How fasting induces gene expression changes and longevity remains unclear. MicroRNAs (miRNAs) are small non-coding RNAs (approximately 22 nucleotides) that repress gene expression, and the expression of several miRNAs has been reported to be altered by fasting. more...

Organism:

Caenorhabditis elegans

Type:

Expression profiling by array

Platform:

GPL200

8 Samples

Download data: CEL

(Submitter supplied) Intermittent fasting (IF), a dietary restriction regimen, extends the lifespans of C. elegans and mammals by inducing gene expression changes. How fasting induces gene expression changes and longevity remains unclear. MicroRNAs (miRNAs) are small non-coding RNAs (approximately 22 nucleotides) that repress gene expression, and the expression of several miRNAs has been reported to be altered by fasting. more...

Organism:

synthetic construct; Caenorhabditis elegans

Type:

Non-coding RNA profiling by array

Platform:

GPL21572

3 Samples

Download data: CEL

(Submitter supplied) mRNA expression profiling of genes regulated by dietary restriction and SEK-1.

Organism:

Caenorhabditis elegans

Type:

Expression profiling by high throughput sequencing

Platform:

GPL13657

12 Samples

Download data: CSV

(Submitter supplied) Mechanical stress by blunt force trauma elicits a distinct and reproducible transcriptional response in C. elegans. Vhp-1, a MAPK phosphatase, regulates a significant portion of these stress-inducible transcripts.

Organism:

Caenorhabditis elegans

Type:

Expression profiling by high throughput sequencing

Platform:

GPL18245

10 Samples

Download data: XLSX

(Submitter supplied) C. elegans exhibit an age-dependent mechanical stress response to blunt force injury. Stress responses are often defined in part by an elicited cellular transcriptional response. We find in C. elegans that mechanical stress by blunt trauma induces a distinct and age-dependent transcriptional program.

Organism:

Caenorhabditis elegans

Type:

Expression profiling by array

Platform:

GPL200

15 Samples

Download data: CEL