GEO DataSets

(Submitter supplied) DICER-like proteins produce small RNAs that silence genes involved in development and defenses against viruses and pathogens. Which DCLs participate in plant-herbivore interactions remains unstudied. We identified four distinct DCL genes and stably silenced their expression by RNAi in Nicotiana attenuata, a model system for the study of plant-herbivore interactions. Silencing DCL1 expression was lethal to the plants. more...

Organism:

Nicotiana attenuata

Type:

Expression profiling by array

Platform:

GPL13527

9 Samples

Download data: TXT

(Submitter supplied) Identification of nectary expressed genes

Organism:

Nicotiana attenuata

Type:

Expression profiling by array

Platform:

GPL13527

6 Samples

Download data: TXT

(Submitter supplied) Identification of transcripts that change expression in corollas of Nicotiana attenuata plants with reduced expression of the transcription factor HD20.

Organism:

Nicotiana attenuata

Type:

Expression profiling by array

Platform:

GPL13527

6 Samples

Download data: TXT

(Submitter supplied) Idenfication and characterization of oscillating transcripts after elicitation with oral secretions from the sepcialist herbivore, Manduca sexta larvae

Organism:

Nicotiana attenuata

Type:

Expression profiling by array

Platform:

GPL13527

134 Samples

Download data: TXT

(Submitter supplied) Identification of transcripts that change expression in Nicotiana attenuata plants with reduced expression of lectin receptor kinase 1 after elicitation with oral secretions of Manduca sexta larvae.

Organism:

Nicotiana attenuata

Type:

Expression profiling by array

Platform:

GPL13527

6 Samples

Download data: TXT

(Submitter supplied) Identification of significantly altered transcripts in Nicotiana attenuata plants with reduced MYC2 expression after induction with oral secretions of Manduca sexta larvae.

Organism:

Nicotiana attenuata

Type:

Expression profiling by array

Platform:

GPL13527

6 Samples

Download data: TXT

(Submitter supplied) WT plants (at the five-leaf stage) were infected with PSTVd strain NB by agroinfiltration. Three weeks post infection (3 wpi) young leaves were collected from infected and uninfected (control) WT plants and used in a custom genome-wide gene expression microarray (4x 180K Sureprint G3 microarray, Agilent)

Organism:

Nicotiana benthamiana

Type:

Expression profiling by array

Platform:

GPL21946

3 Samples

Download data: TXT

(Submitter supplied) Identification of transcripts that change expression in roots of Nicotiana attenuata plants with reduced expression of HSPRO and in association with Piriformospora indica.

Organism:

Nicotiana attenuata

Type:

Expression profiling by array

Platform:

GPL13527

6 Samples

Download data: TXT

(Submitter supplied) The osdcl4-1 mutant exhibits much severer developmental defects than dcl4 in Arabidopsis, suggesting that Os DCL4 may process broader substrates in rice. By deep sequencing of small RNAs from different tissues of wild types and osdcl4-1, we revealed that 21-nucleotide siRNAs were largely dependent on Os DCL4. Besides several tasiRNA loci reported in Arabidopsis and rice, over one thousand 21-nucleotide and several dozen 24-nucleotide phased siRNA (phasiRNA) clusters were identified in panicles but not in seedlings and grains. more...

Organism:

Oryza sativa

Type:

Non-coding RNA profiling by high throughput sequencing

Platform:

GPL9147

6 Samples

Download data: TXT

(Submitter supplied) Oviposition by lepidopteran herbivores on Nicotiana attenuata primes plant defence responses that are induced by the feeding larvae. While oviposition by both the generalist Spodoptera exigua and the specialist Manduca sexta primes the production of defensive phenylpropanoids, their larvae are differentially affected. We investigate here the impact of prior oviposition on the transcriptome and phytohormone levels of plants that were later attacked by larvae to find regulatory signals of this priming. more...

Organism:

Nicotiana attenuata

Type:

Expression profiling by array

Platform:

GPL25245

27 Samples

Download data: TXT

(Submitter supplied) Identification of transcripts that change expression in Nicotiana attenuata plants with reduced expression of JAZi in flowers

Organism:

Nicotiana attenuata

Type:

Expression profiling by array

Platform:

GPL21138

12 Samples

Download data: TXT

(Submitter supplied) Dicer enzymes function at the core of RNA silencing to defend against exogenous RNA, or as an endogenous mechanism of gene regulation. Plant DICER-LIKE4 (DCL4) performs dual functions, acting in antiviral defense, as well as in development via the biogenesis of tasiR-ARFs. These small RNAs play an essential role in the grasses and act to spatially define the expression domain of AUXIN RESPONSE FACTOR3 (ARF3) transcription factors. more...

Organism:

Zea mays

Type:

Non-coding RNA profiling by high throughput sequencing

Platform:

GPL15463

12 Samples

Download data: TXT

(Submitter supplied) Small RNAs play essential regulatory roles in genome stability, development and stress responses in most eukaryotes. Plants encode DICER-LIKE (DCL) RNaseIII enzymes, including DCL1, which produces miRNAs, and DCL2, DCL3 and DCL4, which produce diverse size classes of siRNA. Plants also encode RNASE THREE-LIKE (RTL) enzymes that lack DCL-specific domains and whose function is largely unknown. Small RNA sequencing in plants over-expressing RTL1 or RTL2 or lacking RTL2 revealed that RTL1 over-expression inhibits the accumulation of all types of small RNAs produced by DCL2, DCL3 and DCL4, indicating that RTL1 is a general suppressor of plant siRNA pathways. more...

Organism:

Arabidopsis thaliana

Type:

Non-coding RNA profiling by high throughput sequencing

Platform:

GPL9302

3 Samples

Download data: XLS

(Submitter supplied) Identification of coronatine/jasmonoyl-isoleucine-regulated transcripts and encoded metabolic processes in late stages of Nicotiana attenuata flower development.

Organism:

Nicotiana attenuata

Type:

Expression profiling by array

Platform:

GPL13527

6 Samples

Download data: TXT

(Submitter supplied) This SuperSeries is composed of the SubSeries listed below.

Organism:

Solanum dulcamara

Type:

Expression profiling by array

Platform:

GPL23228

24 Samples

Download data: TXT

(Submitter supplied) Plant resistance traits against insect herbivores are extremely plastic. Plants respond not only to the herbivory itself, but also to oviposition by herbivorous insects. How prior oviposition affects plant responses to larval herbivory is largely unknown. Combining bioassays and defense protein activity assays with microarray analyses and metabolite profiling, we investigated the impact of preceding oviposition on the interaction of Solanum dulcamara with the generalist lepidopteran herbivore Spodoptera exigua at the levels of the plant's resistance, transcriptome and metabolome. more...

Organism:

Solanum dulcamara

Type:

Expression profiling by array

Platform:

GPL23228

12 Samples

Download data: TXT

(Submitter supplied) Plant resistance traits against insect herbivores are extremely plastic. Plants respond not only to the herbivory itself, but also to oviposition by herbivorous insects. How prior oviposition affects plant responses to larval herbivory is largely unknown. Combining bioassays and defense protein activity assays with microarray analyses and metabolite profiling, we investigated the impact of preceding oviposition on the interaction of Solanum dulcamara with the generalist lepidopteran herbivore Spodoptera exigua at the levels of the plant's resistance, transcriptome and metabolome. more...

Organism:

Solanum dulcamara

Type:

Expression profiling by array

Platform:

GPL23228

12 Samples

Download data: TXT

(Submitter supplied) Fatty acid-amino acid conjugates (FACs) in the oral secretion (OS) of the Manduca sexta larvae are necessary and sufficient to elicit the herbivory-specific responses in Nicotiana attenuata, an annual wild tobacco species. We used SuperSAGE combined with 454 sequencing to quantify the early transcriptional changes elicited by the FAC N-linolenoyl-glutamic acid (18:3-Glu) in N. attenuata. The second fully expanded leaf of rosette stage plants were wounded by rolling a fabric pattern wheel three times on each side of the midvein and the wounds were immediately supplied with either 20 uL of 0.02% (v/v) Tween-20/water (solvent control) or 10 uL of synthetic N-linolenoyl-glutamic acid (18:3-Glu; 0.03 nmol/µL in 0.02% (v/v) Tween-20/water). more...

Organism:

Nicotiana attenuata

Type:

Expression profiling by high throughput sequencing

Platform:

GPL9556

2 Samples

Download data: TXT

(Submitter supplied) DICER-like 3 (DCL3) is a major player in heterochromatic 24-nucleotide siRNA and long miRNA biogenesis and mutants have been characterized from Arabidopsis and rice. Here, a tomato DCL3 mutant was generated through the use of trans-activated artificial microRNA and characterized. Global tomato DCL3 (SlDCL3) silencing was induced by crossing the generated responder line (OP:amiRDCL3) with constitutive driver line. more...

Organism:

Solanum lycopersicum

Type:

Non-coding RNA profiling by high throughput sequencing

Platform:

GPL16345

4 Samples

Download data: TXT

(Submitter supplied) Disturbing the biosynthetic pathway of 17-hydroxylgeranyllinalool diterpene glycosides (HGL-DTGs), including silencing NaCYP736A, caused severe plant autotoxicity. This autotoxicity can be restored by co-silencing the upstream biosynthetic genes, like geranyllinalool synthase (GLS). Through comparing the transcriptome data of EV, VIGS-GLS, VIGS-CYP736A and VIGS-GLS&CYP736A, we tried to figure out the potential mechanism of this autotoxicity.

Organism:

Nicotiana attenuata

Type:

Expression profiling by array

Platform:

GPL21138

16 Samples

Download data: TXT