GEO DataSets

(Submitter supplied) This SuperSeries is composed of the SubSeries listed below.

Organism:

Mus musculus

Type:

Expression profiling by array

Platform:

GPL6246

96 Samples

Download data: CEL

(Submitter supplied) We analyzed the transcriptional signatures of mouse bone marrow-derived macrophages (BMDM) at different times after infection with promastigotes of the protozoan parasite Leishmania major. GeneChip Mouse Gene 1.0 ST arrays were used to analyse global changes in gene transcripts to generate a pool of genes that was statistically significant (p-value < 0.05) and a fold change cut-off of 1.5 in at least one of the five infected samples versus non-infected samples.

Organism:

Mus musculus

Type:

Expression profiling by array

Platform:

GPL6246

48 Samples

Download data: CEL, TXT

(Submitter supplied) We analyzed the transcriptional signatures of mouse bone marrow-derived macrophages (BMDM) at different times after infection with promastigotes of the protozoan parasite Leishmania major. GeneChip Mouse Gene 1.0 ST arrays were used to analyse global changes in gene transcripts to generate a pool of genes that was statistically significant (p-value < 0.05) and a fold change cut-off of 1.5 in at least one of the five infected samples versus non-infected samples.

Organism:

Mus musculus

Type:

Expression profiling by array

Platform:

GPL6246

48 Samples

Download data: CEL, TXT

(Submitter supplied) Leishmania (L) are intracellular protozoan parasites which are able to survive and replicate within the harsh and potentially hostile phago-lysosomal environment of mammalian mononuclear phagocytes. A complex interplay then takes place between the macrophage (MΦ) striving to eliminate the pathogen and the parasite struggling for its own survival. To investigate, at the transcriptional level, this host-parasite conflict in the context of monocyte-derived human MΦs (MDM) infection by L. more...

Organism:

Homo sapiens; Leishmania major

Type:

Expression profiling by SAGE

Platforms:

GPL4 GPL6471

3 Samples

Download data

(Submitter supplied) BMDM from BAL/c and C57BL/6 BMDM were stimlated with IFN-g overnight and infected with different Yersinia strains for 3 hrs. Keywords: repeat

Organism:

Mus musculus

Type:

Expression profiling by array

Dataset:

GDS1465

Platform:

GPL339

37 Samples

Download data

Analysis of bone marrow derived macrophages from resistant C57BL/6 or susceptible BALB/c animals, with or without IFN-gamma pretreatment, infected with Yersinia enterocolitica. Results provide insight into the impact of host genetic background and IFN-gamma on Y. enterocolitica infections.

Organism:

Mus musculus

Type:

Expression profiling by array, count, 2 agent, 2 disease state, 2 genotype/variation, 3 strain sets

Platform:

GPL339

Series:

GSE2973

37 Samples

Download data

(Submitter supplied) Protozoan parasites of the genus Leishmania are the causative agent of leishmaniasis, one of the 13 most important tropical diseases. Leishmania persists as endo-parasite in host macrophages, where it uses multiple strategies to manipulate the microbicidal host cell functions and to escape from the host immune system. Understanding how Leishmania interacts with host macrophages during uptake, differentiation, intracellular replication, and release might be the key to develop new drugs in target-directed approaches to treat patient with leishmaniasis. more...

Organism:

Mus musculus; synthetic construct

Type:

Expression profiling by array

Platform:

GPL16384

8 Samples

Download data: CEL

(Submitter supplied) Autophagy generally participates in innate immunity by elimination of intracellular pathogens. However, many of them developed successful strategies to counteract their autolysosomal digestion and lastly to exploit this catabolic cellular process. Protozoan parasites of the genus Leishmania are the causative agent of leishmaniasis, one of the 13 most important tropical diseases. Leishmania persists as endo-parasite in host macrophages, where it uses multiple strategies to manipulate the microbicidal host cell functions and to escape from the host immune system. more...

Organism:

Mus musculus

Type:

Expression profiling by array

Platform:

GPL1261

8 Samples

Download data: CEL

(Submitter supplied) The goal of this experiment was to identify genes which are differentially regulated between Leishmania major- infected granuloma macrophages from mice resistant (C57 Bl/6) and susceptible (BALB/c) to experimental leishmaniasis

Organism:

Mus musculus

Type:

Expression profiling by array

Platform:

GPL81

12 Samples

Download data: CEL

(Submitter supplied) Macrophages possess the hallmark feature of plasticity, allowing them to undergo a dynamic transition between M1 and M2 polarized phenotypes. The aim of the present study was to screen for differentially expressed genes (DEGs) that were associated with macrophage polarization. The transcription profiles of three M1 and three M2 samples were obtained using microarray analysis. Based on the threshold of fold-change >2.0 and a p-value < 0.05, we screened a total of 1,253 DEGs, of which 696 were upregulated and 557 downregulated in M1 macrophages compared to that in M2 macrophages. more...

Organism:

Mus musculus

Type:

Expression profiling by array

Platform:

GPL20939

6 Samples

Download data: TXT

(Submitter supplied) In mammals, resident dermal macrophages (MΦs) are subverted by Leishmania (L.) amazonensis amastigotes as host cells permissive for parasite multiplication. These Leishmania are living within a communal parasitophorous vacuole (PV) and are expected to trigger unique MΦ transcriptional signatures. We performed a transcription profiling of mouse MΦs harboring amastigotes to get insights into their reprogramming as host cells for parasite multiplication. more...

Organism:

Mus musculus

Type:

Expression profiling by array

Platform:

GPL1261

4 Samples

Download data: CEL

(Submitter supplied) ABSTRACT Macrophages are essential components of the innate immune system and crucial for pathogen elimination in early stages of infection. We previously observed that bone marrow-derived macrophages (BMM) from C57BL/6 mice exhibited increased killing activity against Burkholderia pseudomallei compared to BMM from BALB/c mice. This effect was particularly pronounced when cells were treated with IFN-γ. more...

Organism:

Mus musculus

Type:

Expression profiling by array

Platform:

GPL6246

12 Samples

Download data: CEL

(Submitter supplied) Tumor-associated macrophages (TAMs) represent alternatively activated (M2) macrophages that support tumor growth. Previously, we have described a special LYVE-1(+) M2 TAM subset in vitro and in vivo; gene profiling of this TAM subset identified MS4A8A as a novel TAM molecule expressed in vivo by TAM in mammary carcinoma and malignant melanoma. In vitro, Ms4a8a mRNA and MS4A8A protein expression was strongly induced in bone marrow-derived macrophages (BMDMs) by combining M2 mediators (IL-4, glucocorticoids) and tumor-conditioned media (TCM). more...

Organism:

Mus musculus

Type:

Expression profiling by array

Platform:

GPL15041

6 Samples

Download data: CEL

(Submitter supplied) Analysis of bone marrow derived macrophages (BMDM) incubated with dexamethasone&IL4 (Dexa+IL4), B16F1 tumor conditioned medium (cmB16), and B16F1 tumor conditioned medium supplemented with dexamethasone&IL4 (cmB16+dexa+IL4). Results allow detection of genes that require synergistic stimulation of tumor factors and Th2 cytokines.

Organism:

Mus musculus

Type:

Expression profiling by array

Platform:

GPL6526

9 Samples

Download data: CEL

(Submitter supplied) Monocyte derived dendritic cells (MDDC) were infected with Leishmania major or Leishmania donovani parasites and collected at 4, 8, and 24 hours post-infection to analyze the differential effects those parasite species have on human host cell gene expression over time.

Organism:

Homo sapiens

Type:

Expression profiling by array

Platform:

GPL16025

35 Samples

Download data: PAIR

(Submitter supplied) To determine the modulation of gene expression of mouse BMDCs in the presence of living intracellular Leishmania amazonensis amastigotes at 24 hr post infection.

Organism:

Mus musculus

Type:

Expression profiling by array

Platform:

GPL1261

9 Samples

Download data: CEL

(Submitter supplied) Leishmania RNA virus 1 (LRV1) is a double stranded RNA (dsRNA) virus found in some strains of the human protozoan parasite Leishmania, the causative agent of leishmaniasis, a neglected tropical disease. Interestingly, the presence of LRV1 inside Leishmania constitutes an important virulence factor which worsens leishmaniasis outcome in a type I interferon (type I IFN) dependent manner and contributes to treatment failure. more...

Organism:

Mus musculus

Type:

Expression profiling by high throughput sequencing

Platform:

GPL17021

30 Samples

Download data: TXT

(Submitter supplied) Leishmania parasites cause cutaneous leishmanina (CL), a pathologic disease characterized by disfiguring, ulcerative skin lesions. Both parasite and host gene expression following infection with various Leishmania species has been investigated in vitro, but global transcriptional analysis following L. major infection in vivo is lacking. Thus, we conducted a comprehensive transcriptomic profiling study combining bulk RNA sequencing and single-cell RNA sequencing (scRNA-Seq) to identify global changes in gene expression in vivo following L. more...

Organism:

Mus musculus

Type:

Expression profiling by high throughput sequencing

Platform:

GPL19057

10 Samples

Download data: TXT

(Submitter supplied) RNA-seq analysis was performed in ear samples from naïve control and L. major infected mice. Using scRNASeq, we investigated the transcriptomic changes within individual cell types present in leishmanial lesion. Transcriptomic analysis through scRNASeq revealed 35 distinct cell populations which include both resident and recruited cell types following L. major infection. In addition, our scRNASeq analysis confirms a significant recruitment of various immune cell types such as macrophages, inflammtory monocytes, neutrophils, dendritic cells, CD4 and CD8 T cells in the infected ears that are seen at higher frequencies compared to naive controls. more...

Organism:

Mus musculus

Type:

Expression profiling by high throughput sequencing

Platform:

GPL19057

8 Samples

Download data: ZIP

(Submitter supplied) SUMOylation, a posttranslational modification, regulates protein function by covalent attachment of small ubiquitin-like modifier (SUMO) proteins to a lysine (Lys) residue on target proteins. Here we use ML-792, a selective SUMO-activating enzyme (SAE) inhibitor, to inhibit global SUMOylation in macrophages derived from bone marrow of C57BL/6 mice. Mouse bone marrow cells isolated from 6–10 weeks C57BL/6 mice were cultured in IMDM medium supplemented with 10% (vol/vol) FBS and 10 ng/mL of macrophage colony-stimulating factor (M-CSF). more...

Organism:

Mus musculus

Type:

Expression profiling by high throughput sequencing

Platform:

GPL24247

4 Samples

Download data: TXT