GEO DataSets

(Submitter supplied) Amniotic fluid (AF) is a complex biological material that provides a unique window into the developing human. Residual AF supernatant contains cell-free fetal RNA. The objective of this study was to develop an understanding of the AF core transcriptome by identifying the transcripts ubiquitously present in the AF supernatant of euploid midtrimester fetuses. We detected 476 well-annotated genes present in all twelve AF samples. more...

Organism:

Homo sapiens

Type:

Expression profiling by array

Platform:

GPL570

12 Samples

Download data: CEL

(Submitter supplied) To identify molecular pathophysiologic changes and novel disease mechanisms specific to myelomeningocele by analyzing AFS cfRNA in fetuses with open myelomeningocele.

Organism:

Homo sapiens

Type:

Expression profiling by array

Platform:

GPL570

20 Samples

Download data: CEL

(Submitter supplied) Amniotic fluid was colelcted during midtrimester from 30 women and at term gestation from 68 women. Cell free RNA was profiled by HTA 2.0 arrays to study the effect of gestational age and other relevant convariates on gene expression and splicing during normal pregnancy.

Organism:

Homo sapiens

Type:

Expression profiling by array

Platform:

GPL17586

98 Samples

Download data: CEL

(Submitter supplied) The objective of this study was to identify the tissue expression patterns and biological pathways enriched in term amniotic fluid cell-free fetal RNA by comparing functional genomic analyses of term and second-trimester amniotic fluid supernatants. There were 2,871 significantly differentially regulated genes. In term amniotic fluid, tissue expression analysis showed enrichment of salivary gland, tracheal, and renal transcripts as compared with brain and embryonic neural cells in the second trimester. more...

Organism:

Homo sapiens

Type:

Expression profiling by array

Platform:

GPL570

16 Samples

Download data: CEL

(Submitter supplied) This SuperSeries is composed of the SubSeries listed below.

Organism:

Homo sapiens

Type:

Expression profiling by array; Expression profiling by high throughput sequencing

Platforms:

GPL570 GPL11154

10 Samples

Download data: CEL, FPKM_TRACKING

(Submitter supplied) The second trimester fetal transcriptome can be assessed based on cell-free RNA found within the amniotic fluid supernatant. The objective of this study was to compare the suitability of two technologies for profiling the human fetal transcriptome: RNA-Seq and expression microarray. Comparisons were based on total numbers of gene detected, rank-order gene expression, and functional genomic analysis. more...

Organism:

Homo sapiens

Type:

Expression profiling by array

Platform:

GPL570

5 Samples

Download data: CEL

(Submitter supplied) Background: Turner syndrome, a common sex chromosome aneuploidy, has characteristics and malformations associated with the phenotype. Fetal amniotic fluid is a complex biological material that could contribute to the understanding Turner syndrome pathogenesis. Global gene expression analysis of Turner syndrome fetal amniotic fluid supernatant was utilized to identify organ systems and specific genes that may play a role in the pathophysiologic changes that are seen in individuals with Turner syndrome. more...

Organism:

Homo sapiens

Type:

Expression profiling by array

Dataset:

GDS5211

Platform:

GPL570

10 Samples

Download data: CEL

Analysis of second trimester amniotic fluid from Turner syndrome and euploid female fetuses. Turner syndrome is a sex chromosome aneuploidy generally due to monosomy X. Results provide insight into molecular mechanisms underlying Turner syndrome pathogenesis.

Organism:

Homo sapiens

Type:

Expression profiling by array, transformed count, 2 disease state, 2 genotype/variation sets

Platform:

GPL570

Series:

GSE58435

10 Samples

Download data: CEL

(Submitter supplied) Objective: Amniotic fluid (AF) is a proximal fluid to the fetus containing higher amounts of cell-free fetal RNA/DNA than maternal serum, thereby making it a promising source for novel biomarker discovery of fetal development and maturation. Our aim was to compare AF transcriptomic profiles at different time points in pregnancy to demonstrate unique genetic signatures that would serve as potential biomarkers indicative of fetal maturation. more...

Organism:

Homo sapiens

Type:

Expression profiling by high throughput sequencing

Platform:

GPL11154

16 Samples

Download data: XLSX

(Submitter supplied) Amniotic fluid is critical for the development of foetus, which provides the appropriate conditions for foetus growth. Besides its presumed traditional roles of providing movement place and protecting from crushing, amniotic fluid is considered to have more important roles in the nervous development of foetus respecting nervous related compositions and close contact with the skin of foetus. Peripheral nervous sensory system is the feeling basis for the body with neuritis anchoring on the surface of skin. more...

Organism:

Mus musculus

Type:

Non-coding RNA profiling by array

Platform:

GPL20632

3 Samples

Download data: GPR

(Submitter supplied) In order to characterize the differences between second trimester Down syndrome (DS) and euploid fetuses, we compared gene expression in uncultured amniotic fluid supernatant samples. We identified individually differentially expressed genes via paired t-tests in the matched samples, and a set of differentially expressed genes on chromosome 21 using Gene Set Enrichment Analysis. Functional pathway analysis of the resulting genes highlighted the importance of oxidative stress, ion transport, and G-protein signaling in the DS fetuses.

Organism:

Homo sapiens

Type:

Expression profiling by array

Platform:

GPL570

14 Samples

Download data: CEL

(Submitter supplied) We have discovered that GBS significantly remodels its transcriptome in response to exposure to human amniotic fluid. A large number of the affected genes are of unknown function, which means that much remains to be learned about the full influence of amniotic fluid on GBS. The majority of the observed changes in transcripts affects genes involved in basic bacterial metabolism and is connected to AF composition and nutritional requirements of the bacterium. more...

Organism:

Streptococcus agalactiae

Type:

Expression profiling by array

Platform:

GPL6923

9 Samples

Download data: CEL, CHP

(Submitter supplied) We determined dynamic behavior of S. agalactiae transcriptome during growth in THY medium and detected growth phase regulated genes

Organism:

Streptococcus agalactiae

Type:

Expression profiling by array

Platform:

GPL6923

12 Samples

Download data: CEL

(Submitter supplied) Amniotic fluid (AF) volume (AFV) is determined primarily by the rate of intramembranous (IM) transport of AF across the amnion. Intramembranous transport is characterized as vesicular endocytotic and transcytotic processes regulated by fetal urine-derived stimulators and AF inhibitors. Our objectives were to utilize a large-scale multiomics approach to decipher the vesicular transport pathways in the amnion and to identify potential transport regulators in AF and fetal urine. more...

Organism:

Ovis aries

Type:

Expression profiling by high throughput sequencing

Platform:

GPL15670

16 Samples

Download data: TXT

(Submitter supplied) To describe normal cardiac and brain development during late first and early second trimester in human fetuses using microarray and pathways analysis and the creation of a corresponding “normal” database. RNA from recovered tissues was used for transcriptome analysis with Affymetrix 1.0 ST microarray chip. From the amassed data we investigated differences in cardiac and brain development within the 10-18 GA period dividing the sample by GA in three groups: 10-12 (H1), 13-15(H2) and 16-18(H3) weeks. more...

Organism:

Homo sapiens

Type:

Expression profiling by array

Platform:

GPL6244

6 Samples

Download data: CEL

(Submitter supplied) In cattle, harnessing information from the maternal blood to predict fetal health and development is attractive yet scarcely explored. We hypothesised that variations in fetal growth at the end of the organogenesis period impact on the molecular profile of fetal organs and is reflected in the maternal blood transcriptome. The objectives were to determine the transcriptomic modifications in maternal blood and in fetal liver, gonads, and heart according to fetal weight and to model a molecular signature based on the fetal organs allowing the prediction of fetal weight from the maternal blood transcriptome. more...

Organism:

Bos taurus

Type:

Expression profiling by high throughput sequencing

Platform:

GPL26012

36 Samples

Download data: TXT

(Submitter supplied) we analyzed the transcriptomics of single cell of amniotic fluid both from normal and trisomy 18 individuals by using 10x Genomics.

Organism:

Homo sapiens

Type:

Expression profiling by high throughput sequencing

Platform:

GPL24676

5 Samples

Download data: XLS

(Submitter supplied) Little is known about the repertoire of nonhuman primate kidney genes expressed throughout development. The present work establishes an understanding of the primate renal transcriptome at different stages of fetal development and defines the framework of gene expression in the context of renal maturation, which provides a basis for identifying deviations in transcriptome expression resulting from suboptimal conditions during renal development. more...

Organism:

Homo sapiens; Papio hamadryas

Type:

Expression profiling by array

Platforms:

GPL571 GPL96

14 Samples

Download data: CEL