GEO DataSets

(Submitter supplied) Although decades of research have established that androgen is essential for spermatogenesis, androgen’s mechanism of action remains elusive. This is in part because only a few androgen-responsive genes have been definitively identified in the testis. Here, we report that microRNAs—small, noncoding RNAs—are one class of androgen-regulated trans-acting factors in the testis. Specifically, by using androgen suppression and androgen replacement in mice, we show that androgen regulates the expression of several microRNAs in Sertoli cells. more...

Organism:

Mus musculus

Type:

Non-coding RNA profiling by array

Platform:

GPL8824

4 Samples

Download data: TXT

(Submitter supplied) To unravel the molecular mechanisms mediating the effects of androgens on spermatogenesis, testicular gene expression was compared in mice with a Sertoli cell-selective androgen receptor knockout (SCARKO) and littermate controls on postnatal d 10. At this age testicular cell composition is still comparable in SCARKOs and controls. Microarray analysis identified 692 genes with significant differences in expression. more...

Organism:

Mus musculus

Type:

Expression profiling by array

Dataset:

GDS1366

Platform:

GPL8321

10 Samples

Download data: CEL

(Submitter supplied) To unravel the molecular mechanisms mediating the effects of androgens on spermatogenesis, testicular gene expression was compared in mice with a Sertoli cell-selective androgen receptor knockout (SCARKO) and littermate controls on postnatal d 10. At this age testicular cell composition is still comparable in SCARKOs and controls. Microarray analysis identified 692 genes with significant differences in expression. more...

Organism:

Mus musculus

Type:

Expression profiling by array

Dataset:

GDS1367

Platform:

GPL8321

10 Samples

Download data: CEL

Analysis of testis from Sertoli cell-selective androgen receptor knockouts (SCARKO) between postnatal day 8 and 20. Results provide insight into molecular consequences of selective absence of androgen action in Sertoli cells at onset of spermatogenesis.

Organism:

Mus musculus

Type:

Expression profiling by array, count, 5 age, 2 strain sets

Platform:

GPL8321

Series:

GSE2259

10 Samples

Download data: CEL

Analysis of testis from Sertoli cell-selective androgen receptor knockouts (SCARKO) at postnatal day 10. Results provide insight into molecular consequences of selective absence of androgen action in Sertoli cells at onset of spermatogenesis.

Organism:

Mus musculus

Type:

Expression profiling by array, count, 2 strain sets

Platform:

GPL8321

Series:

GSE2260

10 Samples

Download data: CEL

(Submitter supplied) Rhox5, the founding member of the reproductive homeobox on the X chromosome (Rhox) gene cluster, encodes a homeodomain-containing transcription factor that is selectively expressed in Sertoli cells, where it promotes the survival of male germ cells. To identify Rhox5-regulated genes, we generated 15P-1 Sertoli cell clones expressing physiological levels of Rhox5 from a stably transfected expression vector. more...

Organism:

Mus musculus

Type:

Expression profiling by array

Platform:

GPL1261

4 Samples

Download data: CEL

(Submitter supplied) Spermatogenesis is dependent primarily on testosterone action on the Sertoli cells. Although the identification of testosterone-regulated target genes in Sertoli cells has been approached using microarray analysis to compare gene expression in mice with androgen receptor (AR) elimination in the Sertoli cells (SCARKO) and wild type mice, the analysis has been complicated by alteration of germ cell composition of the testis when pubertal or adult mice were used and differences in Sertoli-cell gene expression from those in adult when prepubertal mice were used. more...

Organism:

Mus musculus

Type:

Expression profiling by array

Platform:

GPL1261

6 Samples

Download data: CEL

(Submitter supplied) Spermatogonial stem cells are quiescent, undergo self-renewal or differentiating divisions, thereby forming the cellular basis of spermatogenesis. This cellular development is orchestrated by follicle-stimulating hormone (FSH), through the production of Sertoli cell-derived factors, and by Leydig cell-released androgens. Here, we investigate the transcriptional events induced by Fsh in a steroid-independent manner on the restart of zebrafish (Danio rerio) spermatogenesis ex vivo, using testis from adult males where type A spermatogonia were enriched by estrogen treatment in vivo. more...

Organism:

Danio rerio

Type:

Expression profiling by high throughput sequencing

Platform:

GPL18413

12 Samples

Download data: TXT

(Submitter supplied) We combine synchronization of spermatogenesis, cytological analyses and single-cell RNA-seq (scRNA-seq) in the Sertoli cell androgen receptor knockout (SCARKO) mutant and control mice, and demonstrate that SCARKO mutant spermatocytes exhibited normal expression and localization of key protein markers of meiotic prophase events, indicating that initiation of meiotic prophase is not androgen dependent, whereas scRNA-seq analysis revealed a molecular transcriptomic block in an early meiotic prophase state (leptotene/zygotene) in mutant germ cells, and identified several misregulated genes in SCARKO Sertoli cells, many of which have been previously implicated in male infertility.

Organism:

Mus musculus

Type:

Expression profiling by high throughput sequencing

Platform:

GPL21103

8 Samples

Download data: TXT

(Submitter supplied) Phagocytic clearance of apoptotic germ cells by Sertoli cells is vital for germ cell development and differentiation. Using a tissue-specific miRNA transgenic mouse model, we show that interaction between miR-471-5p and autophagy member proteins regulates clearance of apoptotic germ cells via LC3-associated phagocytosis (LAP).

Organism:

Mus musculus

Type:

Expression profiling by high throughput sequencing

Platform:

GPL13112

2 Samples

Download data: TXT