GEO DataSets

(Submitter supplied) Sputum cells collected before (visit 2) and after (visit 4) allergen challenge in asthma patients were isolated and RNA purified for analysis on gene expression arrays. Human subject recruitment part of NIH sponsored protocol as part of the Eosinophil Program Project Grant (PI: Dr. Nizar Jarjour)

Organism:

Homo sapiens

Type:

Expression profiling by array

Platform:

GPL14550

12 Samples

Download data: TXT

(Submitter supplied) Segmental allergen challenge increases the percentage of eosinophils in bronchoalveolar lavage (BAL) cells. Mepolizumab, an anti-IL-5 therapeutic antibody, decreases the number of eosinophils in bronchoalveolar lavages (BAL). The use of both procedures allows to define genes that are either expressed by eosinophils or dependent on eosinophil presence in the airways.

Organism:

Homo sapiens

Type:

Expression profiling by array

Platform:

GPL6244

8 Samples

Download data: CEL

(Submitter supplied) The pro-inflammatory cytokine IL-6 is elevated in both blood and airways in asthma. IL-6 signaling is regulated by its receptor composed of two proteins, IL-6R and GP130, that are found in both membrane and soluble forms. The interaction of IL-6 with soluble IL-6R (sIL-6R) can trigger IL-6 trans-signaling in cells such as epithelial cells and fibroblasts, which express GP130, but do not produce sufficient IL-6R. more...

Organism:

Homo sapiens

Type:

Expression profiling by high throughput sequencing

Platform:

GPL16791

24 Samples

Download data: XLSX

(Submitter supplied) The eosinophil transcriptome analysis indicated a robust transcription change in eosinophils following allergen challenge in the lung.

Organism:

Mus musculus

Type:

Expression profiling by array

Dataset:

GDS5289

Platform:

GPL6246

7 Samples

Download data: CEL, CHP, TXT

Analysis of lung eosinophils of sensitized animals exposed intranasally to ovalbumin to induce eosinophilia. Eosinophilia, higher than normal eosinophil count, is a feature of allergic asthma. Results provide insight into the molecular response of eosinophils to allergen challenge.

Organism:

Mus musculus

Type:

Expression profiling by array, transformed count, 2 agent sets

Platform:

GPL6246

Series:

GSE57757

7 Samples

Download data: CEL, CHP

(Submitter supplied) Asthma influences brain health both functionally (emotions) and structurally (neurodegeneration). Although airway inflammation is proposed to initiate afferent communications with the brain, the associated patterns of airway inflammation and gene-expression profiles are not established. Here, we investigate associations of gene expression in response to allergen challenge in the lung with functional magnetic resonance imaging (fMRI) in the brain.

Organism:

Homo sapiens

Type:

Expression profiling by high throughput sequencing

Platform:

GPL16791

52 Samples

Download data: CSV

(Submitter supplied) Bronchial Epithelial Cells were isolated processed as described (Chu et al., 2002 and Zhao et al., 2011). The objective of the study was to identify differentially expressed genes between normal control (NC), mild-moderate asmathic (notSA) and severe asthmatic (SA) patients. For demographics data, contact Dr.Sally Wenzel (wenzelse@upmc.edu)

Organism:

Homo sapiens

Type:

Expression profiling by array

Platform:

GPL4133

154 Samples

Download data: TXT

(Submitter supplied) We used single-cell RNA sequencing (scRNA-seq) paired with DNA-barcoded antibodies to see if it allows to distinguish immune cells in different anatomical compartments in the lung akin to flow cytometry. To do this, we infected Balb/c animals with non-typeable Haemophilus influenzae (NTHi) and administered barcoded anti-CD45 antibody intratracheally and intravenously to label airway and vascular immune cells respectively. more...

Organism:

Mus musculus

Type:

Expression profiling by high throughput sequencing

Platform:

GPL24247

2 Samples

Download data: CSV, MTX, TSV, TXT