GEO DataSets

(Submitter supplied) A number of six proteins were selected during immunoscreening and further analyses. The proteins were divided in silico into overlapping 15-mer oligopeptides with an overlap of 11 residues. The microarrays were incubated with different antibodies to K. pneumoniae, C. jejuni and S. aureus.

Organism:

Campylobacter jejuni subsp. jejuni NCTC 11168 = ATCC 700819; Klebsiella pneumoniae

Type:

Protein profiling by protein array

Platforms:

GPL17961 GPL17960

20 Samples

Download data: TXT

(Submitter supplied) Sequences of 11 amino acids belonging to the KPN_00363 protein and KPN_00459 protein from Klebsiella pneumoniae MGH 78578 which was previously identified as potentially immunogenic was analyzed via alanine scanning to narrow down the significant amino acid residues within the sequence.

Organism:

Klebsiella pneumoniae

Type:

Protein profiling by protein array

Platform:

GPL19102

12 Samples

Download data: TXT

(Submitter supplied) Screening of 14 novel proteins derived from Klebsiella pneumoniae MGH 78578 identified prior via screening of cDNA libraries. The full-length proteins were attached using a specific HaloTag to their corresponding ligand surface, HaloLink. Screening was performed using two different polyclonal antibodies to Klebsiella pneumoniae (Acris AP00792PU-N and Abcam ab20947) and detection achieved by Goat polyclonal to rabbit IgG conjugated with Chromeo-546 (Abcam ab60317). more...

Organism:

Klebsiella pneumoniae

Type:

Protein profiling by protein array

Platforms:

GPL17963 GPL17962

16 Samples

Download data: TXT

(Submitter supplied) The screening of a cDNA derived expression library of Klebsiella pneumoniae MGH 78578 expressed in E.coli using a fusion construct and specific HaloTag interaction to a modified surface is shown. Thus, 1536 different clones were screened including positive (ompA, mdh) and negative (pyrC, gapA) reference proteins. The goal of the screening was to identify potential novel immunogenic proteins from K. pneumoniae by selecting clones showing a high signal intensity in comparison to the known antigens used as positve markers. Afterwards, the most promising clones were sequenced to identify the gene and corresponding protein and these proteins were then investigated further. Consequently, 14 novel immunogenic proteins could be identified.

Organism:

Klebsiella pneumoniae

Type:

Protein profiling by protein array

4 related Platforms

11 Samples

Download data: TXT

(Submitter supplied) A short sequence of 11 amino acids belonging to the cj0669 protein from Campylobacter jejuni NCTC 11168 which was previously identified as potentially immunogenig was analyzed via alanine scanning to narrow down the significant amino acid residues within the sequence.

Organism:

Campylobacter jejuni subsp. jejuni NCTC 11168 = ATCC 700819

Type:

Protein profiling by protein array

Platform:

GPL17115

8 Samples

Download data: TXT

(Submitter supplied) A short sequence of 11 amino acids belonging to the cj0669 protein from Campylobacter jejuni NCTC 11168, which was previously identified as potentially immunogenic, was analyzed via alanine scanning to narrow down the significant amino acid residues within the sequence.

Organism:

Campylobacter jejuni subsp. jejuni NCTC 11168 = ATCC 700819

Type:

Protein profiling by protein array

Platform:

GPL16889

10 Samples

Download data: GPR

(Submitter supplied) A number of seven proteins were selected during immunoscreening and further analyses. The proteins were in silico divided into overlapping 15-mer oligopeptides with an overlap of 11 residues. The microarrays were incubated with different antibodies to C. jejuni, Escherichia coli and Salmonella enterica.

Organism:

Campylobacter jejuni subsp. jejuni NCTC 11168 = ATCC 700819

Type:

Protein profiling by protein array

Platforms:

GPL16885 GPL16884

18 Samples

Download data: GPR

(Submitter supplied) Screening of 22 novel proteins derived from Campylobacter jejuni NCTC 11168 identified prior via screening of cDNA libraries. The full-length proteins were attached using a specific HaloTag to their corresponding ligand surface, HaloLink. Screening was performed using three different polyclonal antibodies to Campylobacter jejuni and detection was achieved by goat polyclonal antibody to rabbit IgG conjugated with Chromeo-546. more...

Organism:

Campylobacter jejuni subsp. jejuni NCTC 11168 = ATCC 700819

Type:

Protein profiling by protein array

Platforms:

GPL16881 GPL16880 GPL16882

28 Samples

Download data: GPR

(Submitter supplied) The screening of a cDNA derived expression library of Campylobacter jejuni NCTC 11168 expressed in E. coli using a fusion construct and specific HaloTag interaction to a modified surface is shown. 1536 different clones were screened including positive (hisJ, cjaA, peb1a) and negative (argC, pyrC, gapA) reference proteins. The goal of the screening was to identify potential novel immunogenic proteins from C. more...

Organism:

Campylobacter jejuni subsp. jejuni NCTC 11168 = ATCC 700819

Type:

Protein profiling by protein array

4 related Platforms

12 Samples

Download data: GPR

(Submitter supplied) This SuperSeries is composed of the SubSeries listed below.

Organism:

Campylobacter jejuni subsp. jejuni NCTC 11168 = ATCC 700819

Type:

Protein profiling by protein array

11 related Platforms

76 Samples

Download data: GPR