Similar studies for GEO DataSets (Select 200056989) - GEO DataSets

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Items: 20

Select item 2000569891.

Genome-wide identification of HIF-1 and HIF-2 binding sites in hypoxic human macrophages alternatively activated by IL-10

(Submitter supplied) Primary human macrophages with a HIF-1alpha or HIF-2alpha knockdown were pretreated with IL-10 for 16h and afterwards for 4h additionaly under hypoxi (1% O2), RNA was isolated usind the Qiagen RNAeasy Kit and cDNA synthesis wos done using Ambion WT Expression Kit. Expression was compared to si control under control conditions.

Organism:: Homo sapiens

Type:: Expression profiling by array

Platform:: GPL6244
36 Samples

Download data: CEL

Series

Accession:: GSE56989

ID:: 200056989

Select item 2000708862.

Genome-wide mapping of Hif-1α binding sites in zebrafish

(Submitter supplied) This SuperSeries is composed of the SubSeries listed below.

Organism:: Danio rerio

Type:: Genome binding/occupancy profiling by high throughput sequencing; Expression profiling by array

Platforms:: GPL10816 GPL14875
10 Samples

Download data: BW, TXT

Series

Accession:: GSE70886

ID:: 200070886

PubMed Full text in PMC Similar studies Analyze with GEO2R

Select item 2000708853.

Genome-wide mapping of Hif-1α binding sites in zebrafish [microarray]

(Submitter supplied) Analysis of gene expression changes in the von Hippel Lindau when mutant compared to wild-type at 4dpf using a whole genome microarray expression profiling. The von Hippel Lindau mutant displays a systemic hypoxic response under normoxic conditions. We performed single-colour microarrays to identify the gene expression changes which underpin the hypoxic phenotype. We used 3 biological replicates from both mutant and control, followed by analysis using Limma to identify significant gene expression changes. more...

Organism:: Danio rerio

Type:: Expression profiling by array

Platform:: GPL10816
6 Samples

Download data: TXT

Series

Accession:: GSE70885

ID:: 200070885

PubMed Full text in PMC Similar studies Analyze with GEO2R

Select item 2000707274.

Genome-wide mapping of Hif-1α binding sites in zebrafish [ChIP-seq]

(Submitter supplied) Analysis of the binding sites of Hif-1α in both wild-type and von Hippel Lindau mutant zebrafish lines at 4dpf by ChIP linked next generation sequencing. The von Hippel Lindau mutant displays a systemic hypoxic response under normoxic conditions. Results show the extent of Hif-1α binding to the genome, and provide a basis for analysis of the transcriptional response to genetically induced hypoxia in zebrafish.

Organism:: Danio rerio

Type:: Genome binding/occupancy profiling by high throughput sequencing

Platform:: GPL14875
4 Samples

Download data: BW, TXT

Series

Accession:: GSE70727

ID:: 200070727

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Select item 2000816355.

Genome-wide mapping of HIF-2α binding under normoxia in human bronchial epithelial cells (BEAS-2B)

(Submitter supplied) Growing number of cancer (stem) cells and stem cells were described to accommodate constituent active HIF-2α under normoxia. Previous study of hypoxia effect may have obscured some of normoxic HIF-2α functions as hypoxia inducible features. Our interest in study of protective potential of HIFs in lung cells led us to discover pseudohypoxia functions of HIF-2α under normoxia in human bronchial epithelial cells which exhibit pluripotency related markers and features. more...

Organism:: Homo sapiens

Type:: Genome binding/occupancy profiling by high throughput sequencing

Platform:: GPL16288
2 Samples

Download data: BED

Series

Accession:: GSE81635

ID:: 200081635

PubMed Full text in PMC Similar studies SRA Run Selector

Select item 2001205276.

Copper Regulation of HIF-1 Transcription Activity

(Submitter supplied) This SuperSeries is composed of the SubSeries listed below.

Organism:: Homo sapiens

Type:: Expression profiling by high throughput sequencing; Genome binding/occupancy profiling by high throughput sequencing

Platform:: GPL20795
20 Samples

Download data: BW

Series

Accession:: GSE120527

ID:: 200120527

PubMed Similar studies

Select item 2001205247.

Copper Regulation of HIF-1 Transcription Activity [ChIP-seq]

(Submitter supplied) Copper (Cu) regulates hypoxia-inducible factor-1 (HIF-1) transcription activity by affecting the selectivity of HIF-1α targeting to the promoters of the affected genes. Here, we made an effort to provide a comprehensive understanding of Cu regulation of the selectivity of HIF-1α targeting across genome. We used tetraethylenepentamine (TEPA), a Cu selective chelator, to reduce Cu content in the cells. more...

Organism:: Homo sapiens

Type:: Genome binding/occupancy profiling by high throughput sequencing

Platform:: GPL20795
8 Samples

Download data: BW

Series

Accession:: GSE120524

ID:: 200120524

PubMed Similar studies SRA Run Selector

Select item 2001205238.

Copper Regulation of HIF-1 Transcription Activity [RNA-seq]

Organism:: Homo sapiens

Type:: Expression profiling by high throughput sequencing

Platform:: GPL20795
12 Samples

Download data: TXT

Series

Accession:: GSE120523

ID:: 200120523

Select item 2000277929.

Human monocyte-derived macrophages polarized by GM-CSF or M-CSF

(Submitter supplied) Identification of genes differentially expressed between human monocyte-macrophages generated in the presence of either GM-CSF (termed M1) or M-CSF (termed M2)

Organism:: Homo sapiens

Type:: Expression profiling by array

Platform:: GPL11010
6 Samples

Download data: TXT

Series

Accession:: GSE27792

ID:: 200027792

Select item 20003908910.

Genome-wide binding sites of Hypoxia inducible factor 1 (HIF1) and histone modifications

(Submitter supplied) We report the high-throughput profilings of HIF1 and histone modifications in human umbilical vein endothelial cells (HUVEC). By obtaining over two billion bases of sequence from chromatin immunoprecipitated DNA, we generated genome-wide chromatin-state maps of HUVEC under normoxia and hypoxia. We find that HIF1binds to not only to transcriptional starting sites but also enhancer regions and that HIF1 binding sites were overlapped with lysine 4 trimethylatio, monomethylation and lysine 27 acetylation . more...

Organism:: Homo sapiens

Type:: Genome binding/occupancy profiling by high throughput sequencing

Platform:: GPL9052
8 Samples

Download data: BED, WIG

Series

Accession:: GSE39089

ID:: 200039089

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Select item 20003593211.

HIF1 is a master regulator of the adaptive gene expression to hypoxia.

(Submitter supplied) Total 23 samples were derived from [1] HUVEC treated in the absence (0h) or presence of hypoxia (1, 2, 4, 8, 12, and 24 hrs) to determine hypoxia-regulated gene in endothelial cells, [2] control siRNA or HIF1α siRNA transfected HUVEC cells treated in the absence or presence of hypoxia, [3] control siRNA or KDM3A siRNA transfected HUVEC cells treated in the absence or presence of hypoxia, [4] ChIP-seq data for HIF1 binding sites and histone modifications under normoxia and hypoxia in endothelial cells.

Organism:: Homo sapiens

Type:: Expression profiling by array

Platform:: GPL570
15 Samples

Download data: CEL

Series

Accession:: GSE35932

ID:: 200035932

PubMed Full text in PMC Similar studies Analyze with GEO2R

Select item 20000202012.

Differential Gene Up-regulation by HIF-1a and HIF-2a in HEK293T.

(Submitter supplied) Cell lines. HEK293 human embryonic kidney cells were obtained from ATCC (American Type Culture Collection, Manassas, VA) and were maintained in Dulbecco's modified Eagle's medium supplemented with 10% fetal bovine serum (Heat inactivated, Hyclone, Logan, Utah) and 1X antibiotics/glutamine (100 units/ml penicillin, 100ug/ml streptomycin, 292ug/ml L-Glutamine sulfate, all from Invitrogen Corp, Carlsbad, CA.), under either hypoxic (1% O2) or normoxic (21% O2) conditions at 37C in a tissue culture incubator. more...

Organism:: Homo sapiens

Type:: Expression profiling by array

Dataset:: GDS2018
Platform:: GPL1528
10 Samples

Download data

Series

Accession:: GSE2020

ID:: 200002020

Select item 201813.

Hypoxia-inducible factors-1 and -2 overexpression effect on embryonic kidney cells

Analysis of HEK293T embryonic kidney cells subjected to hypoxia or overexpressing hypoxia-inducible factor (HIF)-1, degradation resistant HIF-1, or HIF-2. Results provide insight into the respective roles of HIF-1 and HIF-2 in the cellular response to hypoxia.

Organism:: Homo sapiens

Type:: Expression profiling by array, log2 ratio, 4 protocol sets

Platform:: GPL1528
Series:: GSE2020
10 Samples

Download data

DataSet

Accession:: GDS2018

ID:: 2018

Select item 20012088714.

Inherent DNA binding specificities of the HIF-1α and HIF-2α transcription factors in chromatin

(Submitter supplied) This SuperSeries is composed of the SubSeries listed below.

Organism:: Homo sapiens

Type:: Genome binding/occupancy profiling by high throughput sequencing; Expression profiling by high throughput sequencing

Platform:: GPL20301
84 Samples

Download data: BED, BIGWIG, TXT

Series

Accession:: GSE120887

ID:: 200120887

PubMed Full text in PMC Similar studies

Select item 20012088615.

Inherent DNA binding specificities of the HIF-1α and HIF-2α transcription factors in chromatin (RNA-seq)

(Submitter supplied) Hypoxia inducible factor (HIF) is the major transcriptional regulator of cellular responses to hypoxia. The two principal HIF-a isoforms, HIF-1a and HIF-2a, are progressively stabilized in response to hypoxia and form heterodimers with HIF-1b to activate a broad range of transcriptional responses. Here we report on the pan-genomic distribution of isoform-specific HIF binding in response to hypoxia of varying severity and duration, and in response to genetic ablation of each HIF-a isoform. more...

Organism:: Homo sapiens

Type:: Expression profiling by high throughput sequencing

Platform:: GPL20301
18 Samples

Download data: TXT

Series

Accession:: GSE120886

ID:: 200120886

PubMed Full text in PMC Similar studies Analyze with GEO2R SRA Run Selector

Select item 20012088516.

Inherent DNA binding specificities of the HIF-1α and HIF-2α transcription factors in chromatin (ChIP-seq)

Organism:: Homo sapiens

Type:: Genome binding/occupancy profiling by high throughput sequencing

Platform:: GPL20301
66 Samples

Download data: BED, BIGWIG

Series

Accession:: GSE120885

ID:: 200120885

PubMed Full text in PMC Similar studies SRA Run Selector

Select item 20012107817.

Expression data of lamina propria macrophages from LysMCre and LysMCre;Arntfl/fl mice with DSS-induced acute colitis

(Submitter supplied) Mice with myeloid ARNT deficiency (LysMCre;Arntfl/fl mice) exhibit defective resolution of DSS-induced acute colitis compared to LysMCre mice. This microarray is carried out to compare the phenotype of lamina propria macrophages from the two cohorts, and to identify factors that are disrupted by myeloid ARNT deficiency and can potentially contribute to the defective resolution of acute colitis.

Organism:: Mus musculus

Type:: Expression profiling by array

Platform:: GPL16570
9 Samples

Download data: CEL

Series

Accession:: GSE121078

ID:: 200121078

PubMed Full text in PMC Similar studies Analyze with GEO2R

Select item 20005431818.

Hypoxia-inducible factor 3 is an oxygen-dependent transcription activator and regulates a distinct transcriptional response to hypoxia

(Submitter supplied) This study aimed to identify Hif-1 and -3 regulated genes in zebrafish embryos. RNA samples were prepared from embryos injected with GFP, Hif-1α, and Hif-3α mRNA. The RNA samples were subjected to DNA microarray analysis using the Agilent 4×44 K (V3) Zebrafish Microarray Chip.

Organism:: Danio rerio

Type:: Expression profiling by array

Platform:: GPL14664
9 Samples

Download data: TXT

Series

Accession:: GSE54318

ID:: 200054318

Select item 20019024219.

The hypoxia induced changes in miRNA in RNA-induced silencing complexes and HIFs induced miRNAs in human endothelial cells

(Submitter supplied) What governs the transition between the two HIFs (the HIF switch) and the role of miRNAs in this regulation is not completely clear. Using genome-wide expression studies on the miRNA content of RNA-induced silencing complex (RISC) in HUVECs exposed to hypoxia compared to the global miRNA-Seq analysis revealed dramatic differences between the two. In our analyses, compared the miRNA and mRNA levels in RISC at 2 hours (mainly HIF-1 driven), 8 hours (HIF-1 and HIF-2 elevated), and 16 hours (mainly HIF-2 driven) in a gene ontology context. more...