GEO DataSets

(Submitter supplied) <Background> The pstSCAB operon of Corynebacterium glutamicum, which encodes an ABC transport system for uptake of phosphate (Pi), is induced during the P i starvation response. The two-component regulatory system PhoRS is involved in this response, but partial Pi starvation induction of pstSCAB in a ∆phoRS mutant indicated the involvement of additional regulator(s). Regulation of pstSCAB also involves the global transcriptional regulator GlxR. more...

Organism:

Corynebacterium glutamicum ATCC 13032

Type:

Expression profiling by array

Platform:

GPL19905

2 Samples

Download data: CSV

(Submitter supplied) DNA affinity chromatography with the promoter region of the Corynebacterium glutamicum pck gene, encoding phosphoenolpyruvate carboxykinase (PEPCk), led to the isolation of four transcriptional regulators, i.e., RamA, GntR1, GntR2 and IolR. Determination of the PEPCk activity of the deletion mutants ΔramA, ΔgntR1ΔgntR2, and ΔiolR indicated that RamA represses pck during growth on glucose about twofold, whereas GntR1, GntR2, and IolR activate pck expression about twofold, irrespective whether glucose or acetate served as carbon source. more...

Organism:

Corynebacterium glutamicum ATCC 13032

Type:

Expression profiling by array

Platform:

GPL15451

3 Samples

Download data: GPR

(Submitter supplied) Transcriptional profiling of Corynebacterium glutamicum cells comparing wild-type cells with cg0196 deletion mutant cells by site-specific gene deletion using the non-replicable integration vector. cg0196 is gene conding transcriptional regulator related carbon metabolism.

Organism:

Corynebacterium glutamicum

Type:

Expression profiling by array

Platform:

GPL14656

1 Sample

Download data: GPR

(Submitter supplied) Corynebacterium glutamicum GlxR is a homolog of the cAMP receptor protein. Although over 200 GlxR binding sites in the C. glutamicum genome are predicted in silico, studies on the GlxR physiological function have been hindered by the severe growth defects of a glxR mutant. This study comprehensively identified the GlxR regulon by chromatin immunoprecipitation in conjunction with microarray (ChIP-chip) analyses. more...

Organism:

Corynebacterium glutamicum

Type:

Genome binding/occupancy profiling by array

Platform:

GPL11651

7 Samples

Download data: GPR

(Submitter supplied) In summary, we have identified and characterized FtsR as a transcriptional activator of the essential cell division protein FtsZ in C. glutamicum, providing a novel regulatory player in the process of cell division.

Organism:

Escherichia coli; Corynebacterium glutamicum; Corynebacterium glutamicum ATCC 13032; Bacillus subtilis subsp. subtilis str. 168; Gluconobacter oxydans

Type:

Expression profiling by array

Platform:

GPL16989

3 Samples

Download data: GPR

(Submitter supplied) This SuperSeries is composed of the SubSeries listed below.

Organism:

Corynebacterium glutamicum R

Type:

Expression profiling by array; Genome binding/occupancy profiling by array

Platform:

GPL18839

8 Samples

Download data: TXT

(Submitter supplied) The transcriptional regulator GntR1 downregultates the genes for gluconate catabolism and pentose phosphate pathway in Corynebacterium glutamicum. Gluconate lowers the DNA binding affinity of GntR1, which is probably the mechanism of gluconate-dependent induction of these genes. In addition, GntR1 positively regulates the ptsG, a gene encoding for a major glucose transporter, and the pck, a gene encoding phosphoenolpyruvate carboxykinase. more...

Organism:

Corynebacterium glutamicum R

Type:

Genome binding/occupancy profiling by array

Platform:

GPL18839

2 Samples

Download data: TXT

(Submitter supplied) The transcriptional regulator GntR1 downregulates the genes for gluconate catabolism and pentose phosphate pathway in Corynebacterium glutamicum. Gluconate lowers the DNA binding affinity of GntR1, which is probably the mechanism of gluconate-dependent induction of these genes. In addition, GntR1 positively regulates the ptsG, a gene encoding for a major glucose transporter, and the pck, a gene encoding phosphoenolpyruvate carboxykinase. more...

Organism:

Corynebacterium glutamicum R

Type:

Expression profiling by array

Platform:

GPL18839

6 Samples

Download data: TXT

(Submitter supplied) DNA microarrays were applied to investigate the effect of cpdA deletion (del) or overexpression (over) on the global gene expression of C. glutamicum cells. For overexpression of cpdA the gene was cloned into plasmid pEKEx2 under control of the IPTG-inducible tac promoter, which was induced by addition of 250 µM IPTG.

Organism:

Corynebacterium glutamicum; Gluconobacter oxydans; Escherichia coli; Bacillus subtilis subsp. subtilis str. 168

Type:

Expression profiling by array

Platform:

GPL20268

6 Samples

Download data: GPR

(Submitter supplied) We recently showed that the two-component system (TCS) HrrSA plays a central role in the control of heme homeostasis in the Gram-positive soil bacterium Corynebacterium glutamicum. Here, we characterized the function of another TCS of this organism, ChrSA, which exhibits significant sequence similarity to HrrSA, and provide evidence for cross-regulation of the two systems. In this study ChrSA was shown to be crucial for heme resistance of C. more...

Organism:

Corynebacterium glutamicum ATCC 13032

Type:

Expression profiling by array

Platform:

GPL15451

6 Samples

Download data: GPR

(Submitter supplied) Copper is an essential cofactor for many enzymes but at high concentrations it is toxic for the cell. Copper ion concentrations ≥50 µM inhibited growth of Corynebacterium glutamicum. The transcriptional response to 20 µM Cu2+ was studied using DNA microarrays and revealed 26 genes that showed a ≥3-fold increased mRNA level, including cg3280-cg3289. Several genes in this genomic region code for proteins presumably involved in the adaption to copper-induced stress, e. more...

Organism:

Corynebacterium glutamicum ATCC 13032

Type:

Expression profiling by array

Platform:

GPL9860

9 Samples

Download data: GPR

(Submitter supplied) The response regulator HrrA belonging to the HrrSA two-component system (previously named CgtSR11) is known to be repressed by the global iron-dependent regulator DtxR in Corynebacterium glutamicum. Sequence analysis indicated an involvement of the HrrSA system in heme-dependent gene expression. Growth experiments revealed that the non-pathogenic soil bacterium C. glutamicum is able to use hemin or hemoglobin as sole iron source. more...

Organism:

Corynebacterium glutamicum ATCC 13032

Type:

Expression profiling by array

Platform:

GPL9860

9 Samples

Download data: GPR

(Submitter supplied) This work reports on the identification and molecular characterization of a two-component regulatory system (2CRS), encoded by phoRP in Bifidobacterium breve UCC2003, which controls the response to phosphate (Pi) starvation. The response regulator PhoP was shown to bind to the promoter region of pstSCAB, specifying a predicted Pi transporter system, as well as that of phoU, which specifies a putative Pi-responsive regulatory protein. more...

Organism:

Bifidobacterium breve UCC2003

Type:

Expression profiling by array

Platform:

GPL8878

12 Samples

Download data: TXT

(Submitter supplied) We evaluated how HrrA binding in response to 4 µM heme as initial stimulus. Analysis was performed prior to the addition of heme (T0) and 0.5, 2, 4, 9, and 24 h after the heme pulse (in medium containing no other iron source).

Organism:

Corynebacterium glutamicum

Type:

Genome binding/occupancy profiling by high throughput sequencing

Platform:

GPL25746

6 Samples

Download data: BED

(Submitter supplied) We evaluated how HrrA binding (found by ChAP-Seq) impacts the expression of individual target genes, by analyzing the transcriptome of the C. glutamicum wild type strain (ATCC 13032) as well as a ∆hrrA mutant. RNA-Seq analysis was performed prior to the addition of heme (T0) and 0.5 and 4 h after the heme pulse (in medium containing no other iron source).

Organism:

Corynebacterium glutamicum ATCC 13032

Type:

Expression profiling by high throughput sequencing

Platform:

GPL25416

6 Samples

Download data: TSV

(Submitter supplied) The cAMP-dependent transcriptional regulator GlxR serves as a central hub in the regulatory network of the actinobacterial model organism Corynebacterium glutamicum and controls expression of ~10% of all genes. The consequences of a lowered cAMP level are mostly unkown. A single gene (cyaB) for a cAMP-synthesizing adenylate cyclase was identified and it had been reported that a cyaB mutant grows like the wild type on glucose, but has a strong growth defect in the presence of acetate. more...

Organism:

Corynebacterium glutamicum ATCC 13032

Type:

Expression profiling by array

Platform:

GPL9860

3 Samples

Download data: GPR, XLSX

(Submitter supplied) In this study, we analyzed the regulation of ƴ-aminobutyrate (GABA) utilization in Corynebacterium glutamicum by the PucR-type transcriptional regulator GabR and by alternative nitrogen and carbon sources.

Organism:

Corynebacterium glutamicum

Type:

Expression profiling by high throughput sequencing

Platform:

GPL29058

1 Sample

Download data: XLS

(Submitter supplied) This SuperSeries is composed of the SubSeries listed below.

Organism:

Pseudomonas putida; Corynebacterium glutamicum; Escherichia coli; Bacillus subtilis subsp. subtilis str. 168; Gluconobacter oxydans

Type:

Expression profiling by array

Platform:

GPL22794

8 Samples

Download data: GPR

(Submitter supplied) γ-Aminobutyric acid (GABA) is a non-proteinogenic amino acid and widespread in nature from microorganisms to plants and animals. DNA microarray analysis revealed that the transcription of gabTDP was strongly increased in C. glutamicum wild type grown with GABA and urea compared to the same strain cultivated with glucose and urea. Remarkably, the presence of ammonia partially inhibited growth on GABA, and the reasons for it were also investigated in this study.

Organism:

Pseudomonas putida; Gluconobacter oxydans; Escherichia coli; Corynebacterium glutamicum; Bacillus subtilis subsp. subtilis str. 168

Type:

Expression profiling by array

Platform:

GPL22794

4 Samples

Download data: GPR

(Submitter supplied) γ-Aminobutyric acid (GABA) is a non-proteinogenic amino acid and widespread in nature from microorganisms to plants and animals. DNA microarray analysis revealed that the transcription of gabTDP was strongly increased in C. glutamicum wild type grown with GABA and urea compared to the same strain cultivated with glucose and urea. Remarkably, the presence of ammonia partially inhibited growth on GABA, and the reasons for it were also investigated in this study.

Organism:

Gluconobacter oxydans; Escherichia coli; Corynebacterium glutamicum; Bacillus subtilis subsp. subtilis str. 168; Pseudomonas putida

Type:

Expression profiling by array

Platform:

GPL22794

4 Samples

Download data: GPR