GEO DataSets

(Submitter supplied) We investigated the covS-regulation of S. pyogenes in a hypervirulent M23 strain using RNA-sequencing. The differential gene expression comparison between the covS- mutant and isogenic wild type covS+ identified altered expression of 349 (18%) genes, including a broad spectrum of virulence genes and diverse metabolic genes. The data showed that the strain achieved hypervirulence by enhancing the expression of genes responsible for invasiveness and antiphagocytosis (i.e., hasABC), by abrogating the expression of toxic genes (i.e., speB), and by compromising gene products with dispensable functions (i.e., sfb1). more...

Organism:

Streptococcus pyogenes

Type:

Expression profiling by high throughput sequencing

Platform:

GPL19982

8 Samples

Download data: XLS

(Submitter supplied) We sought to determine how single amino acid changes in the active site of CovS autophosphorylation and subsequent phosphorylation of the response regulator CovR affect the gene expression profile of group A streptococcus

Organism:

Streptococcus sp. 'group A'

Type:

Expression profiling by high throughput sequencing

Platform:

GPL19691

24 Samples

Download data: TXT

(Submitter supplied) We sought to determine how CovRS mutations varying CovR phosphorylation levels affect the gene expression profile of group A streptococcus

Organism:

Streptococcus sp. 'group A'

Type:

Expression profiling by high throughput sequencing

Platform:

GPL19691

32 Samples

Download data: TXT

(Submitter supplied) Two covR mutant derivatives of parental strain MGAS2221 were recovered from mice experimentally infected with MGAS2221 and shown to differ in terms of the number and concentration of secreted proteins. One of the covR mutant strains had a secretion phenotype identical to a covS mutant strain, while the other had a secretion phenotype identical to a constructed covR mutant strain. To further investigate the potential differences between the two covR mutant strains we performed expression microarray analysis.

Organism:

Coxiella burnetii; Borreliella burgdorferi; Yersinia pestis; Staphylococcus aureus; Chlamydia trachomatis; Streptococcus pyogenes

Type:

Expression profiling by array

Platform:

GPL2129

4 Samples

Download data: CEL

(Submitter supplied) Streptococcus pyogenes (group A Streptococcus, GAS) responds to environmental changes in a manner that results in an adaptive regulation of the transcriptome. Global transcriptional regulators are able to integrate important extracellular and intracellular information and are responsible for modulation of the transcriptional network. The roles of several global transcriptional regulators in adaptation and virulence gene expression have been described. more...

Organism:

Streptococcus pyogenes NZ131

Type:

Expression profiling by array

Platform:

GPL11420

40 Samples

Download data: CEL

(Submitter supplied) To determine the impact of CovS inactivation in acapsular GAS (Streptococcus pyogenes) strains

Organism:

Streptococcus pyogenes

Type:

Expression profiling by high throughput sequencing

Platform:

GPL28678

12 Samples

Download data: CSV

(Submitter supplied) JRS4 is our wild-type M6 serotype strain and JRS948 is the isogenic covR strain. Keywords: 2-channel

Organism:

Streptococcus pyogenes

Type:

Expression profiling by array

Platform:

GPL1482

16 Samples

Download data

(Submitter supplied) We performed CovR ChIP-seq analysis in the emm1 strain MGAS2221 and its CovS kinase deficient derivative strain 2221-CovS-E281A. We identified that CovR bound in the promoter regions of nearly all virulence factor encoding genes in the CovR regulon. Additionally, direct CovR binding was observed for numerous genes encoding proteins involved in amino acid metabolism, but we found limited direct CovR binding to genes encoding other transcriptional regulators. more...

Organism:

Streptococcus pyogenes

Type:

Genome binding/occupancy profiling by high throughput sequencing

Platform:

GPL31155

10 Samples

Download data: XLSX

(Submitter supplied) The control of virulence two-component gene regulatory system (CovRS) is critical to the pathogenesis of many medically important streptococci. In emm1 group A streptococci (GAS), CovR directly binds the promoters of numerous GAS virulence factor encoding genes. Elimination of CovS phosphatase activity increases CovR phosphorylation (CovR~P) levels and abrogates GAS virulence. Given the emm type-specific diversity of CovRS function, herein we used ChIP-seq to define global CovR DNA occupancy in the wild-type emm3 strain MGAS10870 (medium CovR~P) and its CovS phosphatase-negative derivative 10870-CovS-T284A (high CovR~P). more...

Organism:

Streptococcus pyogenes

Type:

Genome binding/occupancy profiling by high throughput sequencing

Platform:

GPL28678

11 Samples

Download data: XLSX

(Submitter supplied) Manuscript title: The endopeptidase PepO regulates the SpeB cysteine protease and is essential for the virulence of invasive M1T1 Streptococcus pyogenes. The study investigates the effect of pepO mutation on global gene expression in GAS M1T1 strain 5448.

Organism:

Streptococcus pyogenes

Type:

Expression profiling by array

Platform:

GPL19152

3 Samples

Download data: TXT, XLSX

(Submitter supplied) Group A streptococci (GAS) may engage different sets of virulence strategies, depending on the site of infection and host context. We previously isolated 2 phenotypic variants of a globally disseminated M1T1 GAS clone: a virulent wild-type (WT) strain, characterized by a SpeB(+)/SpeA(-)/Sda1(low) phenotype, and a hypervirulent animal-passaged (AP) strain, better adapted to survive in vivo, with a SpeB(-)/SpeA(+)/Sda1(high) phenotype. more...

Organism:

Streptococcus pyogenes

Type:

Expression profiling by array

Platform:

GPL10950

40 Samples

Download data

(Submitter supplied) We used RNA-Seq to assess whether the rocA gene regulates gene expression in M1 GAS

Organism:

Streptococcus pyogenes

Type:

Expression profiling by high throughput sequencing

Platform:

GPL20656

6 Samples

Download data: XLSX

(Submitter supplied) We used RNA-Seq to assess whether the rocA gene regulates gene expression in M1 GAS

Organism:

Streptococcus pyogenes

Type:

Expression profiling by high throughput sequencing

Platform:

GPL19982

3 Samples

Download data: XLS

(Submitter supplied) The goal of this study were to compare the transcriptoms of CcpA and CovR in group A Streptococcus. Isogenic mutant strains were created in which CcpA and CovR were inactivated alone and in combination. The transcriptomes of the four strains (wild-type, CcpA mutant, CovR mutant, and ccpA-covR double mutant) were then compared.

Organism:

Streptococcus sp. 'group A'; Streptococcus pyogenes

Type:

Expression profiling by array

Platform:

GPL9080

32 Samples

Download data: CEL

(Submitter supplied) Purpose: We used dual RNA-seq to analyze the transcriptomes of serotype M1 S. pyogenes and host skeletal muscle recovered contemporaneously from infected nonhuman primates (NHPs). We identified genes important for necrotizing myositis, made isogenic deletion mutants and performed RNA-seq comparing one of those deletion mutant strains (dahA) grown in vitro and compared to its parental wild-type strain. more...

Organism:

Streptococcus pyogenes; Macaca fascicularis

Type:

Expression profiling by high throughput sequencing

4 related Platforms

54 Samples

Download data: XLSX

(Submitter supplied) GAS is a highly virulent Gram-positive bacterium. For the successful infection GAS express many virulence factors, which are clustered together with transcriptional regulators in distinct genomic regions. Ralp3 is a central regulator of the ERES region. In this study, we investigated the role of Ralp3 in GAS pathogenesis. To characterize the Ralp3 regulatory function on the whole genome level, GAS M49 wild type and Äralp3 mutant strains were comprehensively compared by two colour microarray analysis. more...

Organism:

Streptococcus pyogenes M49 591; Streptococcus pyogenes NZ131

Type:

Expression profiling by array

Platform:

GPL13816

16 Samples

Download data: TXT

(Submitter supplied) Expression of the extensive arsenal of virulence factors by Streptococcus pyogenes are controlled by many regulators, of which covR/S is one of the best characterized and can influence ~15% of the genome. Animal models have established that mutants of CovR/S arise spontaneously in vivo resulting in highly invasive organisms. We analyzed a pharyngeal and a blood isolate of S. pyogenes recovered from the same individual 13 days apart. more...

Organism:

Streptococcus pyogenes

Type:

Expression profiling by array

Platform:

GPL10318

2 Samples

Download data: PAIR, TXT

(Submitter supplied) This study evaluate how catabolite control protein A (CcpA) inactivation affected gene transcript levels in the group A Streptococcus serotype M1 strain MGAS5005 during growth in standard laboratory medium. Keywords: transcriptome analysis

Organism:

Streptococcus pyogenes; Streptococcus sp. 'group A'

Type:

Expression profiling by array

Platform:

GPL6186

16 Samples

Download data: CEL

(Submitter supplied) We used RNA-Seq to assess how RocA regulates gene expression in stationary phase GAS cultures

Organism:

Streptococcus pyogenes

Type:

Expression profiling by high throughput sequencing

Platform:

GPL18784

4 Samples

Download data: TXT

(Submitter supplied) We used RNA-Seq to assess how RocA regulates gene expression in stationary phase cultures of serotype M1 GAS

Organism:

Streptococcus pyogenes

Type:

Expression profiling by high throughput sequencing

Platform:

GPL18784

4 Samples

Download data: TXT