Similar studies for GEO DataSets (Select 200070104) - GEO DataSets

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Items: 20

Select item 2000701041.

(Submitter supplied) Leukemia Inhibitory Factor (LIF) plays an essential role in the maintenance of pluripotency of mouse embryonic stem cells (mESCs). LIF withdrawal induces mESC differentiation. To define noval pluripotent factors downstream of LIF signaling, cDNA microarray was used and seveal well-known pluripotent genes were found to respond to LIF withdrawal, including Klf4, Esrrb, Tbx3, and Prdm14.

Organism:: Mus musculus

Type:: Expression profiling by array

Platform:: GPL1261
4 Samples

Download data: CEL

Series

Accession:: GSE70104

ID:: 200070104

Select item 2000702462.

Bcl3 target genes in mESCs

(Submitter supplied) Bcl3 is expressed in mESCs. ChIP-seq was used to identify its binding pattern and target genes.

Organism:: Mus musculus

Type:: Genome binding/occupancy profiling by high throughput sequencing

Platform:: GPL9185
2 Samples

Download data: XLSX

Series

Accession:: GSE70246

ID:: 200070246

PubMed Similar studies SRA Run Selector

Select item 2000503933.

Genome-wide analysis of gene expression in LIF and LIF plus CHIR99021 in C57BL/6 mouse embryonic stem cells(B6 mESCs)

(Submitter supplied) Analysis of genes induced by CHIR99021 CHIR99021 is a inhibitor of glycogen synthase kinase 3 (GSK3) and can promote B6 mESC sef-renewal when combined with LIF in serum condition.

Organism:: Mus musculus

Type:: Expression profiling by array

Platform:: GPL13302
2 Samples

Download data: TXT

Series

Accession:: GSE50393

ID:: 200050393

PubMed Full text in PMC Similar studies

Select item 2000348874.

Acute depletion of Tet1-dependent 5-hydroxymethylcytosine levels impairs LIF/Stat3 signaling and results in loss of embryonic stem cell identity [MRE-seq]

(Submitter supplied) The TET family of FE(II) and 2-oxoglutarate-dependent enzymes (Tet1/2/3) promote DNA demethylation by converting 5-methylcytosine to 5-hydroxymethylcytosine (5hmC), which they further oxidize into 5-formylcytosine and 5-carboxylcytosine. Tet1 is robustly expressed in mouse embryonic stem cells (mESCs) and has been implicated in mESC maintenance. Here we demonstrate that, unlike genetic deletion, RNAi-mediated depletion of Tet1 in mESCs led to a significant reduction in 5hmC and loss of mESC identity. more...

Organism:: Mus musculus

Type:: Methylation profiling by high throughput sequencing

Platform:: GPL9250
4 Samples

Download data: BED, WIG

Series

Accession:: GSE34887

ID:: 200034887

PubMed Full text in PMC Similar studies SRA Run Selector

Select item 2000348865.

Acute depletion of Tet1-dependent 5-hydroxymethylcytosine levels impairs LIF/Stat3 signaling and results in loss of embryonic stem cell identity [expression profiling]

Organism:: Mus musculus

Type:: Expression profiling by array

Platform:: GPL14661
8 Samples

Download data: CEL

Series

Accession:: GSE34886

ID:: 200034886

PubMed Full text in PMC Similar studies Analyze with GEO2R

Select item 2000342676.

Acute depletion of Tet1-dependent 5-hydroxymethylcytosine levels impairs LIF/Stat3 signaling and results in loss of embryonic stem cell identity

(Submitter supplied) This SuperSeries is composed of the SubSeries listed below.

Organism:: Mus musculus

Type:: Expression profiling by array; Methylation profiling by high throughput sequencing

Platforms:: GPL14661 GPL9250
12 Samples

Download data: BED, CEL, WIG

Series

Accession:: GSE34267

ID:: 200034267

PubMed Full text in PMC Similar studies Analyze with GEO2R

Select item 2000655977.

Genome-wide identification and analysis of mRNA and miRNA expression in MEFs, ESCs, and iPSCs

(Submitter supplied) This SuperSeries is composed of the SubSeries listed below.

Organism:: Mus musculus

Type:: Expression profiling by array; Non-coding RNA profiling by array

Platforms:: GPL15216 GPL6887
24 Samples

Download data: IDAT, TXT

Series

Accession:: GSE65597

ID:: 200065597

PubMed Full text in PMC Similar studies Analyze with GEO2R

Select item 2000655968.

Genome-wide identification and analysis of microRNA expression in MEFs, ESCs, and iPSCs

(Submitter supplied) Microarray analysis of miRNA—Two micrograms of total RNA was extended at the 3′ terminus with a poly(A) tail using poly(A) polymerase. An oligonucleotide tag was then ligated to the poly(A) tail for later fluorescent dye staining. Hybridization was performed overnight on a µParaflo microfluidic chip using a microcirculation pump (Atactic Technologies). On the microfluidic chip, each detection probe consisted of a chemically modified nucleotide coding segment complementary to the target microRNA (miRBase, http://mirbase.org) and a spacer segment of polyethylene glycol to extend the coding segment away from the substrate. more...

Organism:: Mus musculus

Type:: Non-coding RNA profiling by array

Platform:: GPL15216
12 Samples

Download data: TXT

Series

Accession:: GSE65596

ID:: 200065596

PubMed Full text in PMC Similar studies Analyze with GEO2R

Select item 2000655639.

Genome-wide identification and analysis of mRNA expression in MEFs, ESCs, and iPSCs

(Submitter supplied) Microarray analysis of mRNA—RNA was prepared from ESCs (CGR8 and CJ7), MEFs, and iPSCs with a PureLink RNA Mini Kit (Life Technologies). Initial sample quality control was performed with a Nanodrop 8000 (Thermo Scientific). RNA samples with an optical density (OD) 260/280 ratio and an OD 260/230 ratio of 1.8 or higher were used. Samples with an RNA Integrity Number of 7.5 or greater measured with a LabChip Caliper GX (Perkin Elmer) were applied to an Illumina TotalPrep-96 RNA Amplification Kit (Life Technologies) to generate biotinylated and amplified RNA. more...

Organism:: Mus musculus

Type:: Expression profiling by array

Platform:: GPL6887
12 Samples

Download data: IDAT, TXT

Series

Accession:: GSE65563

ID:: 200065563

PubMed Full text in PMC Similar studies Analyze with GEO2R

Select item 20006756210.

The single-stranded DNA binding protein Ssbp3 promotes trophoblast differentiation of mouse embryonic stem cells

(Submitter supplied) Unlimited self-renewal and developmental pluripotency are hallmarks of embryonic stem cells. Both properties are precisely controlled by the extrinsic signals and intrinsic factors and have been extensively investigated. However, factors capable of converting ES cells to extra-embryonic lineages have been poorly studied. Here we found that overexpression of Ssbp3 dramatically up-regulated trophoblast specific markers. more...

Organism:: Mus musculus

Type:: Expression profiling by array

Platform:: GPL1261
4 Samples

Download data: CEL

Series

Accession:: GSE67562

ID:: 200067562

PubMed Full text in PMC Similar studies Analyze with GEO2R

Select item 20000115111.

CEA_SGF:E00004#TetOFF_Stat3F

(Submitter supplied) The goal of this study was to identify the target genes of Stat3 involved in murine ES cell self-renewal. To achieve this goal, we used the Gs2 ES cell line expressing an inducible dominant negative mutant of Stat3 (Stat3F) in wich Y705 is mutated to phenilalanine. The Gs2 ES cells are routinely maintained in the presence of LIF 1000 U/ml and tetracycline 1µg/ml (LIF/ON). In this condition, Stat3F is not expressed and the cells are maintained in an undifferentiated phenotype. more...

Organism:: Mus musculus

Type:: Expression profiling by array

Platforms:: GPL1091 GPL1063
23 Samples

Download data

Series

Accession:: GSE1151

ID:: 200001151

Select item 20000115012.

CEA_SGF:E00005#LIF

(Submitter supplied) In order to identify the genes involved in LIF/gp130 self-renewal response, we analyzed the transcriptome of Gs2 ES cells induced to differentiate upon LIF withdrawal for 16h, 24h and 48h (LIF16, LIF24 and LIF48 respectively)and compared them to that of undifferentiated ES Gs2 cells continuously maintained in the presence of LIF. Since this cell line also carry a tetracycline regulatable dominant negative form of Stat3 (Stat3F, all the experiment was done in the presence of Tetracycline (tet ON) to avoid Stat3F expression. more...

Organism:: Mus musculus

Type:: Expression profiling by array

Platform:: GPL1091
18 Samples

Download data

Series

Accession:: GSE1150

ID:: 200001150

Select item 10000109113.

CEA_SGF:AFU

(Submitter supplied) Chip contains 9216 features (probes and controls): 2014 PCR products from cDNA matrix using primers specific of genes involved in important biological process (apoptosis, cell cycle, stress response). 1684 PCR products from cDNA clones from the I.M.A.G.E consortium (Research Genetics collection). 2014 PCR products from mouse cDNA clones of a substractive bank (myodistrophic versus normal muscle). 1800 PCR products from rat cDNA clones of a substractive bank (atrophied versus normal muscle). more...

Organism:: Mus musculus

5 Series
107 Samples

Download data

Platform

Accession:: GPL1091

ID:: 100001091

PubMed Similar studies

Select item 20009564214.

DNA-binding pluripotency factors and DNA demethylases can cooperate to maintain pluripotent stem cell identity even in the absence of Brd4

(Submitter supplied) This SuperSeries is composed of the SubSeries listed below.

Organism:: Mus musculus

Type:: Genome binding/occupancy profiling by high throughput sequencing

Platform:: GPL17021
46 Samples

Download data: BW

Series

Accession:: GSE95642

ID:: 200095642

PubMed Full text in PMC Similar studies

Select item 20008876015.

DNA-binding pluripotency factors and DNA demethylases can cooperate to maintain pluripotent stem cell identity even in the absence of Brd4 [RNA-seq]

(Submitter supplied) Histone acetylation and the acetyl-lysine reader Brd4 have recently been implicated in embryonic stem cell (ESC) proliferation and self-renewal. We found that naïve pluripotent ESCs exhibit increased incorporation of glucose-derived carbons onto acetylated histones and elevations in H3K9ac and Brd4 recruitment at pluripotency gene promoters. Surprisingly, both H3K9 acetyltransferases, GCN5 and PCAF, and Brd4 recruitment were dispensable for proliferation, self-renewal and pluripotency of naïve ESCs. more...

Organism:: Mus musculus

Type:: Expression profiling by high throughput sequencing

Platform:: GPL19057
12 Samples

Download data: TXT

Series

Accession:: GSE88760

ID:: 200088760

PubMed Full text in PMC Similar studies SRA Run Selector

Select item 20003871916.

Identify the downstream targets of Stat3 by using an engineered mouse ES cell line treated with GCSF and LIF plus PD0325901

(Submitter supplied) To identify downstream targets of Jak/Stat3 pathways without being distracted by differentiation signalings from MEK/ERK pathway, we exploited a engineered B6 cells, which stably stably expressing a chimeric receptor (GRgp-Y118F). The chimeric receptor can induce the phosphorylation of Stat3 by GCSF without activating the MEK/ERK pathway. To mimic the effect of GCSF, the chimeric B6 cells were also treated with LIF plus a selective MEK chemical inhibitor, PD0325901, to induce LIF/Jak/Stat3 but MEK/ERK pathways.

Organism:: Mus musculus

Type:: Expression profiling by array

Platform:: GPL6246
6 Samples

Download data: CEL

Series

Accession:: GSE38719

ID:: 200038719

Select item 20013202517.

Rinf regulates pluripotency network and Tet enzymes in embryonic stem cells (ESCs)

(Submitter supplied) In this study: (1) We have mapped the genome wide binding distribution and enrichment of Rinf/CXXC5 at genes and regulatory regions in mouse ESCs by ChIP-seq using a specific antibody against Rinf. (2) We have examined the role of Rinf in regulation of ESC gene expression programs by performing transcriptomic analysis of Rinf wild type and knockout ESCs by RNA-seq to identify differentially expressed genes. more...

Organism:: Mus musculus

Type:: Expression profiling by high throughput sequencing; Genome binding/occupancy profiling by high throughput sequencing

Platforms:: GPL17021 GPL19057
21 Samples

Download data: BW, TXT

Series

Accession:: GSE132025

ID:: 200132025

PubMed Full text in PMC Similar studies SRA Run Selector

Select item 20000983618.

NFkB target gene microarray during LIF-withdrawal differentiation of mouse embryonic stem cells

(Submitter supplied) An NFkB target gene array (Panomics, Inc., Redwood City, CA) was performed to profile the expression pattern of 107 NFkB-regulated genes in mouse embryonic stem cells. A small scale microarray was carried out using NFkB target gene array kit (Panomics, Inc., Redwood City, CA). A long sense-strand oligonucleotide for each of the 107 human genes that have been previously shown to be regulated by NFkB signaling pathway was spotted in duplicate on the nitrocellulose membrane. more...

Organism:: Mus musculus

Type:: Expression profiling by array

Platform:: GPL6239
2 Samples

Download data: TIFF

Series

Accession:: GSE9836

ID:: 200009836

Select item 20002826219.

CC chemokine ligand 2 and LIF cooperatively promote pluripotency in mouse induced pluripotent cells

(Submitter supplied) The pluripotency of mouse embryonic stem cells (ESC) and induced-pluripotent stem cells (iPSC) can be maintained by feeder cells, which secrete Leukemia Inhibitory Factor (LIF). We found that feeder cells provide a relatively low concentration (25 unit/ml) of LIF, which is insufficient to maintain the ESC/iPSC pluripotency in feeder free conditions. In order to identify additional factors involved in the maintenance of pluripotency, we carried out a global transcript expression profiling of mouse iPSC cultured on feeder cells and in feeder-free (LIF-treated) conditions. more...