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Links from GEO DataSets

Items: 11

1.

Escherichia coli DH5α: Control vs dr1558 expression

(Submitter supplied) Transcriptional profiling of E. coli cells comparing control harboring the empty vector pRadGro (Ec-pR) with E. coli expressing the Deinococcus radiodurans response regulator DR1558 (Ec-1558) Expression of DR1558 conferred to multi-stress tolerance to E. coli.
Organism:
Escherichia coli; Escherichia coli K-12
Type:
Expression profiling by array
Platform:
GPL20675
3 Samples
Download data: GPR
Series
Accession:
GSE70688
ID:
200070688
2.

Antagonistic regulation of motility and other cellular functions by RpoN and RpoS in Escherichia coli

(Submitter supplied) Bacteria generally possess multiple σ factors that, based on structural and functional similarity, divide into two families: σD and σN. Among the seven σ factors in Escherichia coli, six belongs to the σD family. Each σ factor recognizes a group of promoters, providing effective control of differential gene expression. Many studies have shown that σ factors of the σD family compete with each other for function. more...
Organism:
Escherichia coli; Escherichia coli K-12
Type:
Expression profiling by array
Platform:
GPL3154
9 Samples
Download data: CEL, CHP
Series
Accession:
GSE20095
ID:
200020095
3.

Control of RpoS in global gene expression of Escherichia coli in minimal media

(Submitter supplied) RpoS, an alternative sigma factor, is critical for stress response in Escherichia coli. The RpoS regulon expression has been well characterized in rich media that support fast growth and high growth yields. In contrast, though RpoS levels are high in minimal media, how RpoS functions under such conditions has not been clearly resolved. In this study, we compared the global transcriptional profiles of wild type and an rpoS mutant of E. more...
Organism:
Escherichia coli K-12
Type:
Expression profiling by array
Platform:
GPL199
12 Samples
Download data: CEL, CHP
Series
Accession:
GSE12797
ID:
200012797
4.

Time series (T= 0-4 hours) for E. coli K-12 MC4100 after a shift from 23˚C to 37˚C (M9 minimal glycerol medium, exponential phase growth)

(Submitter supplied) We investigated the effect of a temperature shift from 23˚C to 37˚C, mimicking the temperature transition experienced by a microbe as it enters a human host. This strategy allowed the exploration of the kinetics of gene expression changes in immediate response to a temperature upshift and to characterize the suite of genes required to respond to 37˚C in the first few hours. We demonstrate that a significant number of genes are rapidly altered in expression within minutes to hours after a temperature shift. more...
Organism:
Escherichia coli
Type:
Expression profiling by array
Platform:
GPL6540
9 Samples
Download data: GPR, XLSX
Series
Accession:
GSE165794
ID:
200165794
5.

BW25113 H202 and delta rpoS persister cells vs. stationary phase BW25113 wild-type

(Submitter supplied) E. coli K-12 BW25113 persister cells generated via H202 pre-treatment and deletion of rpoS, relative to BW25113 wild-type stationary phase gene expression. Persister cells were generated following exposure to ampicillin 20 ug/mL.
Organism:
Escherichia coli
Type:
Expression profiling by array
Platform:
GPL3154
3 Samples
Download data: CEL, CHP
Series
Accession:
GSE34028
ID:
200034028
6.

Persistence of BW25113 mqsR producing MqsR 2-1 vs. producing wild-type MqsR with ampicillin

(Submitter supplied) Persisters are a subpopulation of metabolically-dormant cells in biofilms that are resistant to antibiotics; hence, understanding persister cell formation is important for controlling bacterial infections. Previously we discerned that MqsR and MqsA of Escherichia coli are a toxin/antitoxin pair that influence persister cell production via their regulation of Hha, CspD, and HokA. Here, to gain more insights into the origin of persisters, we used protein engineering to increase the toxicity of toxin MqsR by reasoning it would be easier to understand the effect of this toxin if it were more toxic. more...
Organism:
Escherichia coli; Escherichia coli K-12
Type:
Expression profiling by array
Platform:
GPL3154
2 Samples
Download data: CEL, CHP
Series
Accession:
GSE31054
ID:
200031054
7.

Expression data from ethanol-tolerant E. coli strain and wild type under ethanol stress

(Submitter supplied) Cellular tolerance toward ethanol is a complex phenotype involved many genes, and hard to be improved by manipulating individual genes. We previously established exogenous global regulator IrrE mutants that confer Escherichia coli with significantly enhanced tolerance to stresses, including ethanol. In order to elucidate the mechanism for enhancement of ethanol tolerance in the mutants and to identify new genes and pathways that can be possible targets for engineering of ethanol tolerance, we carried out comparative transcriptomic and proteomic analyses with the representative strains E1 and E0 (harboring the ethanol-tolerant mutant E1 of IrrE and the wild type IrrE, respectively). more...
Organism:
Escherichia coli
Type:
Expression profiling by array
Platform:
GPL3154
6 Samples
Download data: CEL
Series
Accession:
GSE30441
ID:
200030441
8.

12 microarrays for cis-DCE mineralization and new stress genes

(Submitter supplied) Metabolically-engineered Escherichia coli has been used previously to degrade the ubiquitous pollutant cis-1,2-dichloroethylene (cis-DCE), and the impact of the metabolic engineering was assessed by investigating the changes in the proteome. Here, genome-wide transcriptome analysis was performed to confirm that a strong heat shock and/or oxidative stress occurs during enhanced cis-DCE mineralization. more...
Organism:
Escherichia coli; Escherichia coli K-12
Type:
Expression profiling by array
Platforms:
GPL199 GPL3154
12 Samples
Download data: CEL, CHP
Series
Accession:
GSE13698
ID:
200013698
9.

Functional Genomic Study of Exogenous n-Butanol Stress in Escherichia coli

(Submitter supplied) n-Butanol has been proposed as an alternative biofuel to ethanol, and both Escherichia coli and Saccharomyces cerevisiae have been engineered to produce it. Unfortunately, n-butanol is more toxic than ethanol to these organisms. To understand the basis for its toxicity, cell wide studies were conducted at the transcript, protein and metabolite levels to obtain a global view of the n-butanol stress response. more...
Organism:
Escherichia coli
Type:
Expression profiling by array
Platform:
GPL8811
12 Samples
Download data: TXT
Series
Accession:
GSE16973
ID:
200016973
10.

Transcriptomic analysis of a putative LysR family regulator, a putative Dps, and a cystine importer disrupted mutants in radiation resistant bacterium Deinococcus geothermalis

(Submitter supplied) Here, we compared gene expression level by RNA-Seq analysis among a wild-type (WT) Deinococcus geothermalis strain, a dgeo_2840 of LysR family member gene (Δdgeo_2840), a putative Dps dgeo_0257 (Δdgeo_0257), and a cystine importer dgeo_1986-1987 gene disrupted strain (Δdgeo_1986-1987). From this transcriptomic data, we detected dgeo_2840, dgeo_0257, and dgeo_1986-1987 controlling genes which were somehow up-regulated and/or down-regulated. more...
Organism:
Deinococcus geothermalis DSM 11300
Type:
Expression profiling by high throughput sequencing
Platform:
GPL28637
4 Samples
Download data: TXT
Series
Accession:
GSE151903
ID:
200151903
11.

Microarray analysis of the wildtype and recombinant E. coli strain containing an ncRNA nfiS to H2O2 shock

(Submitter supplied) The aim of this study was to investigate the expressional changes profiles of the wildtype and recombinant Escherichia coli strain which contains an non-coding RNA nfiS from the nitrogen-fixing Pseudomonas stutzeri A1501 after hydrogen peroxide shock, so as to lay a theoretical foundation for further clarifying the mechanism of this ncRNA to enhance the resistance ability of E.coli to oxidative stress. more...
Organism:
Escherichia coli
Type:
Expression profiling by array
Platform:
GPL3154
6 Samples
Download data: CEL, CHP
Series
Accession:
GSE124807
ID:
200124807
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