GEO DataSets

(Submitter supplied) Recently, extra-cellular components, such as serum and exosome, attract more and more attention as biomarker candidates for mammalian health. However, the contribution of different blood components to the signature of respective microRNAs (miRNAs) remains unknown, especially in cattle. In this study we aimed to investigate the miRNAs from bovine sera and exosomes, and to provide insights into their future applications. more...

Organism:

Bos taurus

Type:

Non-coding RNA profiling by high throughput sequencing

Platform:

GPL15749

8 Samples

Download data: TXT

(Submitter supplied) Milk is an essential source of nutrients to all mammalian offspring, and is rich in a wide variety of immunological components. Recently reported miRNAs that are present in breast milk and are selectively packaged into the exosomes, a type of membrane vesicles, secreted by most cell types. These exosomal miRNAs could be actively delivered into recipient cells, and play a key role in the regulation of innate and adaptive immunity. more...

Organism:

Bos taurus

Type:

Non-coding RNA profiling by high throughput sequencing

Platform:

GPL15749

8 Samples

Download data: TXT

(Submitter supplied) Purpose: MicroRNAs (miRNAs) are non-coding small miRNA ~22 nucleotides in length and play a vital role in muscle development by binding to messenger RNAs (mRNAs). Large White (LW, a lean type pig) and Meishan pigs (MS, a Chinese indigenous obese breed) have significant postnatal phenotype differences in growth rate, muscle mass and meat quality, and theses difference are programmed during prenatal muscle development.To shed directly light on the miRNA transcriptome difference in prenatal muscles between these two distinct pig breeds. more...

Organism:

Sus scrofa

Type:

Non-coding RNA profiling by high throughput sequencing

Platform:

GPL11429

6 Samples

Download data: XLS

(Submitter supplied) In this study, we determined the miRNA expression profile of bovine alveolar macrophages, using next-generation sequencing strategy. On an Illumina HiSeq 2000 machine, we sequenced 8 miRNA libraries, prepared from small RNA fractions of alveolar macrophages isolated from 8 different healthy animals (Bos taurus). From the data, the potential novel miRNAs were predicted, and the expression levels of the known miRNAs were determined. more...

Organism:

Bos taurus

Type:

Non-coding RNA profiling by high throughput sequencing

Platform:

GPL15749

8 Samples

Download data: TXT

(Submitter supplied) Up to 50% of ovulations go undetected in modern dairy herds due to attenuated oestrus behavior and a lack of high-accuracy methods for detection of fertile oestrus. This significantly reduces overall herd productivity and constitutes a high economic burden to the dairy industry. MicroRNAs (miRNAs) are ubiquitous regulators of gene expression during both health and disease and they have been shown to regulate different reproductive processes. more...

Organism:

Bos taurus

Type:

Non-coding RNA profiling by high throughput sequencing

Platform:

GPL15749

24 Samples

Download data: XLSX

(Submitter supplied) In recent years circulating miRNAs have been proposed as biomarkers of several diseases in humans. However the origin of these potential biomarkers is not always investigated and recent studies have highlighted the importance of selecting biomarkers which are not blood-cell derived to ensure robust performance. The aim of this study was identify circulating miRNAs which are not blood-cell derived (highly expressed in plasma compared to whole blood cells in cow) and investigate the expression of a selection of those in a range of bovine tissues as a follow-up to these results.

Organism:

Bos taurus

Type:

Non-coding RNA profiling by high throughput sequencing

Platform:

GPL15749

8 Samples

Download data: XLSX

(Submitter supplied) To investigate the potential effect of grazing movement on miRNA circulation in cattle, here we profiled miRNA expression in centrifugally prepared exosomes from the plasma of both grazing and housed Japanese Shorthorn cattle. Microarray analysis of the c-miRNAs resulted in detection of a total of 231 bovine exosomal miRNAs in the plasma, with a constant expression level of let-7g across the duration and cattle groups. more...

Organism:

Bos taurus

Type:

Non-coding RNA profiling by array

Platform:

GPL20303

8 Samples

Download data: TXT

(Submitter supplied) Type 1 diabetes mellitus (T1DM) results from an autoimmune attack against the insulin-producing ß cells which leads to chronic hyperglycemia. Exosomes are lipid vesicles derived from cellular multivesicular bodies that are enriched in specific miRNAs, potentially providing a disease-specific diagnostic signature. To assess the value of exosome miRNAs as biomarkers for T1DM, miRNA expression in plasma-derived exosomes was measured. more...

Organism:

Homo sapiens

Type:

Non-coding RNA profiling by array

Platform:

GPL17537

24 Samples

Download data: RCC, TXT

(Submitter supplied) Exosomes are cell-derived vesicles that were found in many biological fluids such as blood, urine, and cultured medium. Exosomes are small vesicles (approximately 100nm in diameter) that contain many functional molecules like cytokines, receptors and regulating RNAs. In this study, we found that uMSC-derived exosomes accelerates wound healing and reduce myoblast formation in vivo. In vitro study showed uMSC-exosomes specific microRNAs take major roles in inhibiting myoblast differentiation of fibroblast. more...

Organism:

Homo sapiens

Type:

Non-coding RNA profiling by high throughput sequencing

Platform:

GPL17303

2 Samples

Download data: TXT

(Submitter supplied) Low fertility remains a leading cause of poor productivity in dairy cattle. In this context, there is significant interest in developing novel tools for accurate early diagnosis of pregnancy. MicroRNAs (miRNAs) are short RNA molecules which are critically involved in regulating gene expression during both health and disease. MiRNAs have been shown to regulate ovarian function, uterine receptivity, embryonic development and placental function. more...

Organism:

Bos taurus

Type:

Non-coding RNA profiling by high throughput sequencing

Platform:

GPL15749

46 Samples

Download data: XLSX

(Submitter supplied) Bone marrow (BM) cells were obtained by flushing the long bones of 8-week old C57BL/6 mice. BM cells were then were plated in macrophage SFM medium (Life Technologies) supplemented with penicillin-streptomycin and CSF-1 (Peprotech, 100 ng/ml) and cultured for one week to allow macrophage differentiation. BMDMs were polarized by adding IL-4 to the medium (40 ng/ml, Peprotech) for 72h or left untreated.

Organism:

Mus musculus

Type:

Expression profiling by high throughput sequencing; Non-coding RNA profiling by high throughput sequencing

Platform:

GPL17021

8 Samples

Download data: GTF, TXT

(Submitter supplied) Background: MiRNAs from body fluids gain more and more attraction as biomarker candidates. Besides serum, patterns from whole blood are increasingly considered as markers for human pathologies. Usually, the contribution of different cell types to the respective signature remains however unknown. In this study we provide insights into the human miRNome of different compounds of the blood including CD3, CD14, CD15, CD19, CD56 positive cells as well as exosomes. more...

Organism:

Homo sapiens

Type:

Non-coding RNA profiling by array

Platform:

GPL16770

66 Samples

Download data: TXT

(Submitter supplied) Since microRNAs (miRNAs) have been associated with eye diseases, our study aims to profile ocular miRNA expression in normal human eye tissue using miRNA-Seq in order to provide a foundation for future disease research. Total RNAs were extracted from normal human ciliary body (CB) (n=7), cornea (n=7), and trabecular meshwork (TM) (n=7) samples using mirVana total RNA isolation kit. The sequencing library was prepared using the Illumina TruSeq Small RNA Sample Prep kit and was sequenced using Illumina MiSeq. more...

Organism:

Homo sapiens

Type:

Non-coding RNA profiling by high throughput sequencing

Platform:

GPL15520

21 Samples

Download data: TXT

(Submitter supplied) To explore the role of miRNAs in intrauterine exosomes in cattle pregnancy, we analyzed the expression levels of the exosomal miRNAs in Xianan cows, a beef cattle breed in China.

Organism:

Bos taurus

Type:

Non-coding RNA profiling by high throughput sequencing

Platform:

GPL24230

6 Samples

Download data: TXT

(Submitter supplied) This SuperSeries is composed of the SubSeries listed below.

Organism:

Homo sapiens

Type:

Expression profiling by array; Non-coding RNA profiling by array

Platforms:

GPL14767 GPL6480

16 Samples

Download data: TXT

(Submitter supplied) Microarray analysis of exosomal and cellular mRNAs in activated and resting primary human T lymphoblasts

Organism:

Homo sapiens

Type:

Expression profiling by array

Dataset:

GDS5639

Platform:

GPL6480

8 Samples

Download data: TXT

(Submitter supplied) Microarray analysis of exosomal and cellular miRNAs in activated and resting primary human T lymphoblasts

Organism:

Homo sapiens

Type:

Non-coding RNA profiling by array

Platform:

GPL14767

8 Samples

Download data: TXT

Analysis of primary T lymphoblasts and derived exosomes after activation with PMA plus ionomycin. Exosomes contain specific RNA species and can be functionally transferred to recipient cells. Results provide insight into molecular mechanisms underlying the selective sorting of RNA into exosomes.

Organism:

Homo sapiens

Type:

Expression profiling by array, transformed count, 2 cell type, 2 protocol sets

Platform:

GPL6480

Series:

GSE50971

8 Samples

Download data: TXT

(Submitter supplied) Cerebrospinal fluid (CSF) miRNAs have emerged as a potential low invasive diagnostic tool for central nervous system malignancies. However, they have not yet been implemented in the clinic since there is no standardized and simple method established. Another problem that it must be considered is that the amount of CSF that can be obtained from patients is very limited, especially from infants. With this in mind, we have compared six different methodologies to establish the best one for the detection of miRNAs using a minimum CSF volume. more...

Organism:

Homo sapiens

Type:

Non-coding RNA profiling by high throughput sequencing

Platform:

GPL16791

15 Samples

Download data: XLS

(Submitter supplied) The role of non-coding RNAs in different biological processes and diseases is continuously expanding. Next-generation sequencing together with the parallel improvement of bioinformatics analyses allows the accurate detection and quantification of an increasing number of RNA species. With the aim of exploring new potential biomarkers for disease classification, a clear overview of the expression levels of common/unique small RNA species among different biospecimens is necessary. more...

Organism:

Homo sapiens

Type:

Non-coding RNA profiling by high throughput sequencing; Expression profiling by high throughput sequencing

Platform:

GPL11154

209 Samples

Download data: TXT