GEO DataSets

(Submitter supplied) Using RNA-seq, we report here that CodY regulation is hierarchically regulated in response to environmental starvation.

Organism:

Bacillus anthracis str. Sterne

Type:

Expression profiling by high throughput sequencing

Platform:

GPL20947

24 Samples

Download data: TXT

(Submitter supplied) Comparison of the B cereus codY deletion mutant with wild type strain (ATCC 14579)

Organism:

Bacillus cereus ATCC 14579

Type:

Expression profiling by array

Platform:

GPL13146

4 Samples

Download data: TXT

(Submitter supplied) CodY is a nutritional regulator mainly involved in amino acid metabolism. It has been extensively studied in Bacillis subtilis and Lactococcus lactis. We investigated the role of CodY in gene regulation and virulence of the human pathogen Streptococcus pneumoniae. We constructed a codY-mutant and examined the effect on gene and protein expression by microarray and 2D DIGE analysis. The pneumococcal CodY-regulon was found to consist predominantly of genes involved in amino acid metabolism, but also several other cellular processes, such as carbon metabolism and iron uptake. more...

Organism:

Streptococcus pneumoniae TIGR4; Streptococcus pneumoniae

Type:

Expression profiling by array

Platform:

GPL5023

7 Samples

Download data: TXT

(Submitter supplied) In this study we focused on understanding the effect of over expression of recombinant protective antigen (rPA) on the Bacillus anthracis metabolism and genes expression with the purpose of improving the production of this recombinant toxin in particular and recombinant proteins in general. To achieve this goal controlled growth of the B. anthracis ames strain transformed with pYS5 (PA producer) plasmid encoding protective antigen and the corresponding empty vector pSW4 (non-producer) were performed and protein production, bacterial growth characteristics and gene transcription pattern were analyzed. more...

Organism:

Bacillus anthracis str. Ames

Type:

Expression profiling by high throughput sequencing

Platform:

GPL24488

18 Samples

Download data: TXT

(Submitter supplied) CodY, a global regulatory protein that monitors the nutrient sufficiency of the environment by responding to the intracellular levels of GTP and the branched-chain amino acids, was previously shown to be a potent repressor of toxin gene expression in Clostridium difficile during growth in rich medium. In the intestinal tract, such derepression of toxin synthesis may lead to destruction of epithelial cells and the liberation of potential nutrients for the bacterium. more...

Organism:

Clostridioides difficile; Clostridioides difficile 630

Type:

Expression profiling by array

Platform:

GPL10731

3 Samples

Download data: TXT

(Submitter supplied) Although genome-wide transcriptional analysis has been used for many years to study bacterial gene expression, many aspects of the bacterial transcriptome remain undefined. A prominent example is antisense transcription, which has been observed in a number of bacteria, though the function of antisense transcripts, and their distribution across the bacterial genome, is still unclear. Single-stranded RNA-seq results revealed a widespread and non-random pattern of antisense transcription covering more than two-thirds of the B. more...

Organism:

Bacillus anthracis

Type:

Expression profiling by high throughput sequencing

Platform:

GPL15353

4 Samples

Download data: TXT

(Submitter supplied) ChIP-seq analysis was used to identify B. dermatitidis genes bound by the GATA transcription factor encoded by SREB during growth as yeast at 37oC

Organism:

Blastomyces dermatitidis ATCC 26199

Type:

Genome binding/occupancy profiling by high throughput sequencing

Platform:

GPL20170

8 Samples

Download data: TXT

(Submitter supplied) This SuperSeries is composed of the SubSeries listed below.

Organism:

Bacillus anthracis

Type:

Expression profiling by high throughput sequencing; Other

Platform:

GPL31186

20 Samples

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(Submitter supplied) Formaldehyde cross-linking RNA immunoprecipitation coupled with illumina sequencing (fRIP-seq) was performed to pinpoint the direct RNA targets of KrrA using a FLAG-tagged KrrA construct that is driven by a constitutive promoter Plgt (pOS1.PlgtkrrA-FLAG).

Organism:

Bacillus anthracis

Type:

Expression profiling by high throughput sequencing; Other

Platform:

GPL31186

8 Samples

Download data: XLSX

(Submitter supplied) To uncover the effects of KrrA regulation on gene transcription and define the bacterial response imposed by this regulation, a transcriptomic study was carried out in which Bacillus anthracis krrA was compared to WT in two growth conditions: LB in the presence or absence of ‘205.

Organism:

Bacillus anthracis

Type:

Expression profiling by high throughput sequencing

Platform:

GPL31186

12 Samples

Download data: XLSX

(Submitter supplied) Although gene expression has been studied in bacteria for decades, many aspects of the bacterial transcriptome remain poorly understood. Transcript structure, operon linkages, and absolute abundance information all provide valuable insights into gene function and regulation, but none has ever been determined on a genome-wide scale for any bacterium. Here we report the first comprehensive and unbiased definition of a bacterial transcriptome under various growth conditions. more...

Organism:

Bacillus anthracis

Type:

Expression profiling by high throughput sequencing

Platforms:

GPL9410 GPL9411

8 Samples

Download data: QUAL, TXT

(Submitter supplied) The High Temperature Requirement (HtrA) chaperone/protease is involved in the stress-response of the anthrax-causing pathogen Bacillus anthracis. Resilience to oxidative stress is essential for manifestation of B. anthracis pathogenicity. We submitt transcriptome data sets detailing global gene expression in B. anthracis wild-type and htrA-disrupted strains following H2O2-induced oxidative stress.

Organism:

Bacillus anthracis str. Vollum

Type:

Expression profiling by high throughput sequencing

Platform:

GPL28578

14 Samples

Download data: TXT