GEO DataSets

(Submitter supplied) We conducted a genome-wide transcriptomic analysis in soybean leaves treated with a short-term (24 h) Pi-deficiency using RNA sequencing (RNA-seq) technology. Two biological replicates of RNA-seq were included for both Pi-sufficient leaves (PSL) and Pi-deficient leaves (PDL), and therefore a total of four libraries were constructed. Using a 2-fold change and a P-value ≤0.05 as the cut-off for selecting the differentially expressed transcripts, we globally identified short-term Pi-stress responsive genes. more...

Organism:

Glycine max

Type:

Expression profiling by high throughput sequencing

Platform:

GPL15008

4 Samples

Download data: XLSX

(Submitter supplied) Phosphorus (P) deficiency is a major limitation for legume crop production. Although overall adaptations of plant roots to P deficiency have been extensively studied, fragmentary information is available in regards to root nodule responses to P deficiency. In this study, genome wide transcriptome analysis was conducted using RNA-seq analysis to investigate molecular mechanisms underlying soybean (Glycine max) nodule adaptation to phosphate (Pi) starvation. more...

Organism:

Glycine max

Type:

Expression profiling by high throughput sequencing

Platform:

GPL25283

6 Samples

Download data: XLSX

(Submitter supplied) This SuperSeries is composed of the SubSeries listed below.

Organism:

Hordeum vulgare

Type:

Expression profiling by high throughput sequencing; Non-coding RNA profiling by high throughput sequencing

Platforms:

GPL28029 GPL22077

20 Samples

Download data

(Submitter supplied) RNA was isolated from 23 old barley plants (shoots), line Rolap. Libraries were prepared using mRNA-Seq Library kit v2 (Lexogen), followed by sequencing of NGS libraries

Organism:

Hordeum vulgare

Type:

Expression profiling by high throughput sequencing

Platform:

GPL28029

6 Samples

Download data: XLSX

(Submitter supplied) RNA was isolated from 23 old barley plants (shoots and roots), line Rolap. We used a modified method that allows for enrichment of small RNAs. Libraries were prepared using TruSeq Small RNA Library Preparation Kit (Illumina), followed by sequencing of NGS libraries.

Organism:

Hordeum vulgare

Type:

Non-coding RNA profiling by high throughput sequencing

Platform:

GPL28029

12 Samples

Download data: XLSX

(Submitter supplied) RNA was isolated from 23 old barley plants (shoots and roots), line Rolap. PARE libraries were constructed for both barley organs, followed by sequencing of NGS libraries.

Organism:

Hordeum vulgare

Type:

Non-coding RNA profiling by high throughput sequencing

Platform:

GPL22077

2 Samples

Download data: FASTA, XLSX

(Submitter supplied) Proton toxicity is one of the major environmental stresses limiting crop production, and becomes increasingly serious because of anthropogenic activities. To understand acid tolerance mechanisms, the plant growth, mineral nutrient accumulation and global transcriptome changes in soybean (Glycine max) in response to long-term acid stress were investigated. Results showed that acid stress significantly inhibited soybean root growth, but exhibited slight effects on the shoot growth. more...

Organism:

Glycine max

Type:

Expression profiling by high throughput sequencing

Platform:

GPL24376

6 Samples

Download data: TXT

(Submitter supplied) The purpose of this experiment was to identify any genes differentially expressed between the Soybean Near Isogenic Lines iron efficient Clark and iron inefficient IsoClark under iron sufficient hydroponic conditions. These genes would represent constituative genetic differences between the NILs. Keywords: Near Isogenic Line Comparison of Unstressed Soybean Plants

Organism:

Glycine max

Type:

Expression profiling by array

Platform:

GPL3015

6 Samples

Download data: TXT

(Submitter supplied) This experiment was designed to identify candidate genes related to iron deficiency chlorosis in soybeans that are differentially expressed between near isogenic lines developed for their differential tolerance to low iron conditons. Keywords: Stress Response Analysis

Organism:

Glycine max

Type:

Expression profiling by array

Platform:

GPL1013

6 Samples

Download data: GPR

(Submitter supplied) Seed germination is important to soybean (Glycine max) growth and development, ultimately affecting soybean yield. A lower seed field emergence has been the main hindrance for breeding soybeans low in phytate. Although this reduction could be overcome by additional breeding and selection, the mechanisms of seed germination in different low phytate mutants remain unknown. In this study, we performed a comparative transcript analysis of two low phytate soybean mutants (TW-1 and TW-1-M), which have the same mutation, a 2 bp deletion in GmMIPS1, but show a significant difference in seed field emergence.

Organism:

Glycine max

Type:

Expression profiling by high throughput sequencing

Platform:

GPL21998

18 Samples

Download data: TXT

(Submitter supplied) To understand the mechanisms of sexual behaviour in rams, transcriptomic sequencing technology was used to identify differentially expressed genes in key brain tissues We then performed gene expression profiling analysis using data obtained from RNA-seq of hypothalamus (HT), pars tuberalis (PT) and pineal gland (PG) in rams with different sexual behaviours

Organism:

Ovis aries

Type:

Expression profiling by high throughput sequencing

Platform:

GPL27721

36 Samples

Download data: TXT

(Submitter supplied) Purpose: The goals of this study are to compare differentially expressed transcripts in roots and leaves of spinach plants grown under nitrogen replete and deplete conditions using transcriptome profiling (RNA-seq)

Organism:

Spinacia oleracea

Type:

Expression profiling by high throughput sequencing

Platform:

GPL28198

12 Samples

Download data: TXT

(Submitter supplied) Despite the potential of the endoplasmic reticulum (ER) stress response to accommodate adaptive pathways, its integration with other environmental-induced responses is poorly understood in plants. Here, we performed global expression profiling on soybean leaves exposed to polyethylene glycol treatment or to unfolded protein response (UPR) inducers to identify integrated networks between osmotic and ER stress-induced adaptive responses. more...

Organism:

Glycine max

Type:

Expression profiling by array

Platform:

GPL5810

12 Samples

Download data: CSV

(Submitter supplied) Phosphorus (P) is an important macronutrient for plant growth that participates in a series of biological processes. Thus, P deficiency is considered as one of the major constraints limiting crop growth and yield. Although stylo (Stylosanthes) is a dominate tropical legume that displays adaptation to low phosphate (Pi) availability in acid soils, its adaptive mechanisms remain largely unknown. In this study, variation in low P stress tolerance was investigated using two stylo cultivars in hydroponics, which revealed that the stylo RY2 cultivar exhibited higher adaptability to Pi starvation than the RY5 cultivar, as reflected by less reduction in dry weight under P deficient condition, and accompanied by higher P concentrations in shoot and root. more...

Organism:

Stylosanthes guianensis

Type:

Expression profiling by high throughput sequencing

Platform:

GPL28299

6 Samples

Download data: FASTA, TXT

(Submitter supplied) Microarray experiments were performed on the roots and leaves samples seperately using custom based Nimblgen platform (12plex). This is a time-course study. The plants were grown in controlled growth chamber condiditons ambiient to soybean growth and development using a hydrophonics system. The tissues were harvested after 30days of growth in six time points- 0min, 30mins, 1hour, 2hours, 3hours and 5hours of dehydration.

Organism:

Glycine max

Type:

Expression profiling by array

Platform:

GPL17531

36 Samples

Download data: CALLS, PAIR

(Submitter supplied) By sequencing 36 cDNA libraries with Illumina technology, we identified genes differentially expressed in soybean plants in response to water deficit and genes that were either up- or down-regulated in different periods of the day. Of 54,175 predicted soybean genes (Glyma v1.1), 35.52% exhibited expression oscillations in a 24 h period. This number increased to 39.23% when plants were submitted to water deficit. more...

Organism:

Glycine max

Type:

Expression profiling by high throughput sequencing

Platform:

GPL15008

36 Samples

Download data: TXT, XLS

(Submitter supplied) Objective Type 2 diabetes mellitus (T2DM) is one of the high risk factors for sarcopenia. However, the pathogenesis of diabetic sarcopenia has not been fully elucidated. This study obtained transcriptome profiles of gastrocnemius muscle in normal and T2DM rats based on high-throughput sequencing technology, which may provide new ideas for exploring the pathogenesis of diabetic sarcopenia. Methods Twelve adult male Sprague-Dawley rats were randomly divided into Control group and T2DM group, and gastrocnemius muscle tissue was retained for transcriptome sequencing and real-time quantitative polymerase chain reaction (qRT-PCR) 6 months later. more...

Organism:

Rattus norvegicus

Type:

Expression profiling by high throughput sequencing

Platform:

GPL25947

6 Samples

Download data: TXT

(Submitter supplied) Phosphorus is one of the most important macronutrients that is required for plant growth and development. However, stress under low-P conditions has become a limiting factor that affects crop yields and qualities. Plants have developed strategies to cope with this, while few genes associated with low-P tolerance have been identified in soybean. We used microarrays to detail the global programme of gene expression under different phosphorus treatments of two soybean accessions CD and YH with different phosphorus efficiency.

Organism:

Glycine max

Type:

Expression profiling by array

Platform:

GPL21505

24 Samples

Download data: CEL

(Submitter supplied) Infections with bovine viral diarrhea virus (BVDV) contribute significantly to health-related economic losses in the beef and dairy industries and are widespread throughout the world. Severe acute BVDV infection is characterized by a gastrointestinal (GI) inflammatory response. The mechanism of inflammatory lesions caused by BVDV remains unknown. The interstitial cells of Cajal (ICC) network plays a pivotal role as a pacemaker in the generation of electrical slow waves for GI motility, and it is crucial for the reception of regulatory inputs from the enteric nervous system. more...

Organism:

Ovis aries

Type:

Expression profiling by high throughput sequencing

Platform:

GPL19778

6 Samples

Download data: TXT

(Submitter supplied) Transformation of Glycine max with seed-targeted expression vectors via Agrobacterium causes measurable unscripted gene expression changes in the seed transcriptome

Organism:

Glycine max

Type:

Expression profiling by high throughput sequencing

Platform:

GPL15008

36 Samples

Download data: GTF