Similar studies for GEO DataSets (Select 200112827) - GEO DataSets

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Items: 20

Select item 2001128271.

Impact of Dnm3os silencing on bleomycin-induced lung fibrosis

(Submitter supplied) To assess the impact of Dnm3os silencing on lung fibrogenesis, anti-Dnm3os gapmers (5mg/kg, 2 distinct designs: GP DNM3OS-1 and GP DNM3OS-3) or control gapmer (5mg/kg, GP Ctrl) formulated for in vivo delivery was instilled intratracheally 4 days and 2 days before intratracheal administration of bleomycin (1 unit/kg) or PBS as well as 4 days after bleomycin or PBS treatment.

Organism:: Mus musculus

Type:: Expression profiling by array

Platform:: GPL13912
24 Samples

Download data: TXT

Series

Accession:: GSE112827

ID:: 200112827

Select item 2000978342.

The DNM3OS lncRNA is a reservoir of fibromiRs with major functions in fibroblast response to TGF-beta and fibrogenesis

(Submitter supplied) RATIONALE: Given the paucity of effective treatments for Idiopathic Pulmonary Fibrosis (IPF), new insights into the deleterious mechanisms controlling lung fibroblast activation, the key cell type driving the fibrogenic process, are essential to develop new therapeutic strategies. Transforming growth factor β (TGF-β) is the main pro-fibrotic factor, but its inhibition is associated with severe side effects due to its pleiotropic role. more...

Organism:: Homo sapiens; Mus musculus

Type:: Expression profiling by array; Expression profiling by high throughput sequencing; Non-coding RNA profiling by high throughput sequencing

Platforms:: GPL13912 GPL18573 GPL17077
92 Samples

Download data: TXT

Series

Accession:: GSE97834

ID:: 200097834

Select item 2000978333.

TGF-β signature of MRC-5 fibroblasts

(Submitter supplied) Human MRC5 pulmonary fibroblasts (CCL-171) were exposed or not to TGF-β at 10 ng/mL. RNA samples were harvested 24 hours after TGF-β exposition. Two independent experiments were carried out.

Organism:: Homo sapiens

Type:: Expression profiling by array

Platform:: GPL17077
4 Samples

Download data: TXT

Series

Accession:: GSE97833

ID:: 200097833

Select item 2000978324.

Small RNA-Seq analysis of human lung fibroblasts exposed to TGF-β

(Submitter supplied) To assess the transcriptomic response of human lung fibroblast to TGF-β, human MRC5 pulmonary fibroblasts (CCL-171) were exposed to TGF-β at 10 ng/mL. RNA samples were harvested 24 hours after TGF-β exposition. Three independent experiments were carried out.

Organism:: Homo sapiens

Type:: Non-coding RNA profiling by high throughput sequencing

Platform:: GPL18573
6 Samples

Download data: TXT

Series

Accession:: GSE97832

ID:: 200097832

PubMed Similar studies SRA Run Selector

Select item 2000978295.

RNA-Seq analysis of human lung fibroblasts exposed to TGF-β

(Submitter supplied) To assess the transcriptomic response of human lung fibroblast to TGF-β, human MRC5 pulmonary fibroblasts (CCL-171) were exposed or not to TGF-β at 10 ng/mL. RNA samples were harvested 24 hours after TGF-β exposition. Three independent experiments were carried out.

Organism:: Homo sapiens

Type:: Expression profiling by high throughput sequencing

Platform:: GPL18573
6 Samples

Download data: TXT

Series

Accession:: GSE97829

ID:: 200097829

Select item 2000978266.

Impact of CAV1 target site blocker (CAV1 TSB) on bleomycin-induced lung fibrosis

(Submitter supplied) To assess whether CAV1 represents the main target associated with miR-199a-5p profibrotic activity, we designed a CAV1 target site blocker (TBS) to specifically disrupt miR-199a-5p interaction with mCAV1 3’UTR. CAV1 TSB (5mg/kg) or control formulated for in vivo delivery (Control TSB) was instilled intratracheally 4 days and 2 days before intratracheal administration of bleomycin (1 unit/kg) or PBS as well as 4 days after bleomycin or PBS treatment.

Organism:: Mus musculus

Type:: Expression profiling by array

Platform:: GPL13912
16 Samples

Download data: TXT

Series

Accession:: GSE97826

ID:: 200097826

Select item 2000978257.

Impact of miR-199a-5p silencing on bleomycin-induced lung fibrosis

(Submitter supplied) To assess the impact of miR-199a-5p silencing on lung fibrogenesis, LNA-miR-199a-5p (5mg/kg) or control formulated for in vivo delivery was instilled intratracheally 4 days and 2 days before intratracheal administration of bleomycin (1 unit/kg) or PBS as well as 4 days after bleomycin or PBS treatment.

Organism:: Mus musculus

Type:: Expression profiling by array

Platform:: GPL13912
16 Samples

Download data: TXT

Series

Accession:: GSE97825

ID:: 200097825

Select item 2000978248.

Impact of miR-199-5p, miR-199a-3p and miR-214-3p overexpression on human lung fibroblasts

(Submitter supplied) To identify putative novel specific targets of miR-199-5p, miR-199a-3p and miR-214-3p, we overexpressed these miRNAs in human MRC5 pulmonary fibroblasts (CCL-171) using synthetic pre-miRNAs or a synthetic “negative” pre-miRNA control (miR-Neg). RNA samples were harvested 48 hours post-transfection and 3 independent experiments were carried out.

Organism:: Homo sapiens

Type:: Expression profiling by array

Platform:: GPL17077
12 Samples

Download data: TXT

Series

Accession:: GSE97824

ID:: 200097824

Select item 2000978239.

Impact of DNM3OS silencing on human lung fibroblast response to TGF-β

(Submitter supplied) To assess the role of the lncRNA DNM3OS in TGF-β-induced lung fibroblast activation, we transfected in human MRC5 pulmonary fibroblasts (CCL-171) a gapmer designed against DNM3OS for 48h and then exposed these cells to TGF- β (10 ng/mL). RNA samples were harvested 24 hours after TGF-β exposition. Two independent experiments were carried out.

Organism:: Homo sapiens

Type:: Expression profiling by array

Platform:: GPL17077
8 Samples

Download data: TXT

Series

Accession:: GSE97823

ID:: 200097823

Select item 20003481810.

MiR-199a-5p determines fibroblast activation and pulmonary fibrogenesis

(Submitter supplied) Idiopathic pulmonary fibrosis (IPF) is a chronic and often fatal pulmonary disorder characterized by fibroblast proliferation and the excess deposit of extracellular matrix proteins. The etiology of IPF is unknown, but a central role for microRNAs (miRNAs), a class of small non-coding regulatory RNAs, has been recently suggested. We report the upregulation of miR-199a-5p in mouse lungs undergoing bleomycin-induced fibrosis and also in human biopsies from IPF patients. more...

Organism:: Homo sapiens; Mus musculus

Type:: Expression profiling by array; Non-coding RNA profiling by array

Platforms:: GPL13607 GPL7686 GPL13912
48 Samples

Download data: GPR, TXT

Series

Accession:: GSE34818

ID:: 200034818

PubMed Full text in PMC Similar studies Analyze with GEO2R

Select item 20003481511.

Impact of miR-199-5p overexpression and CAV1 silencing on human lung fibroblasts

(Submitter supplied) To identify putative novel specific targets of mir-199-5p, we overexpressed miR-199a-5p as well as miR-21 and a siRNA targeted against CAV1 in human HFL1 pulmonary fibroblasts (CCL-153) by transfecting them with synthetic pre-miRNAs or a synthetic “negative” pre-miRNA as control (miR-Neg). RNA samples were harvested at 48 hours post-transfection and 2 independent experiments were carried out. Additional samples correspond to HFL1 cells treated or not with 10ng/ml TGFbeta for 48 hours in the absence of serum (2 independent experiments).

Organism:: Homo sapiens

Type:: Expression profiling by array

Platform:: GPL13607
14 Samples

Download data: TXT

Series

Accession:: GSE34815

ID:: 200034815

PubMed Full text in PMC Similar studies Analyze with GEO2R

Select item 20003481412.

mRNA reponse to bleomycin instillation

(Submitter supplied) Gene expression analysis of C57BL/6 mice challenged by intratracheal bleomycin instillation: mRNA expression profiles were established from lungs following a 14-days PBS or bleomycin administration.

Organism:: Mus musculus

Type:: Expression profiling by array

Platform:: GPL13912
10 Samples

Download data: TXT

Series

Accession:: GSE34814

ID:: 200034814

PubMed Full text in PMC Similar studies Analyze with GEO2R

Select item 20003481213.

miRNA reponse to bleomycin instillation

(Submitter supplied) Comparison of C57BL/6 (sensitive) and BALB/c (resistant) mice challenged by intratracheal bleomycin instillation: miRNA expression profiles were established from lungs derived from the two strains, following a 7- or 14-days PBS or bleomycin administration.

Organism:: Mus musculus; Homo sapiens

Type:: Non-coding RNA profiling by array

Platform:: GPL7686
24 Samples

Download data: GPR

Series

Accession:: GSE34812

ID:: 200034812

PubMed Full text in PMC Similar studies Analyze with GEO2R

Select item 20010014714.

Global miRNA expression profiling of lung fibroblasts identifies miR-19a-19b-20a subcluster as a suppressor of TGF-beta-associated fibroblast activation in murine pulmonary fibrosis

(Submitter supplied) This SuperSeries is composed of the SubSeries listed below.

Organism:: Mus musculus

Type:: Non-coding RNA profiling by high throughput sequencing; Expression profiling by high throughput sequencing

Platforms:: GPL18635 GPL16331
13 Samples

Download data: TXT

Series

Accession:: GSE100147

ID:: 200100147

PubMed Full text in PMC Similar studies

Select item 20010011615.

Transcriptomic analysis of miR-19a-19b-20a subcluster-overexpressed fibroblasts in bleomycin-induced lung fibrosis

(Submitter supplied) Lung fibroblasts play a pivotal role in pulmonary fibrosis, a devastating lung diseases, by producing extracellular matrix. MicroRNAs (miRNAs) suppress a lot of genes posttranscriptionally, but the dynamics and the role of miRNAs in activated lung fibroblasts in fibrotic lung has been poorly understood. We found miR-19a, 19b and 20a subcluster expression increased in activated lung fibroblasts as the fibrosis progression. more...

Organism:: Mus musculus

Type:: Expression profiling by high throughput sequencing

Platform:: GPL18635
6 Samples

Download data: TXT

Series

Accession:: GSE100116

ID:: 200100116

PubMed Full text in PMC Similar studies SRA Run Selector

Select item 20010011516.

Time-course global miRNA expression profiling on lung fibroblasts isolated from untreated, bleomycin-treated and silica-treated mice

(Submitter supplied) Lung fibroblasts play a pivotal role in pulmonary fibrosis, a devastating lung diseases, by producing extracellular matrix. MicroRNAs (miRNAs) suppress a lot of genes posttranscriptionally, but the dynamics and the role of miRNAs in activated lung fibroblasts in fibrotic lung has been poorly understood. To elucidate these problems, we performed global miRNA expression profiling of lung fibroblasts of bleomycin- and silica-induced fibrotic lungs

Organism:: Mus musculus

Type:: Non-coding RNA profiling by high throughput sequencing

Platform:: GPL16331
7 Samples

Download data: TXT

Series

Accession:: GSE100115

ID:: 200100115

PubMed Full text in PMC Similar studies SRA Run Selector

Select item 20008618617.

miRNA expression data before and after TGF-β treatment and Ago2-associated transcripts in lung fibroblasts

(Submitter supplied) This SuperSeries is composed of the SubSeries listed below.

Organism:: synthetic construct; Homo sapiens

Type:: Other; Non-coding RNA profiling by array

Platforms:: GPL21185 GPL8786
34 Samples

Download data: CEL

Series

Accession:: GSE86186

ID:: 200086186

PubMed Full text in PMC Similar studies Analyze with GEO2R

Select item 20008618518.

miRNA expression data in unstimulated and TGF-β1-stimulated lung fibroblasts

(Submitter supplied) Lung fibroblasts play an important role in extracellular matrix homeostasis and this process is mainly regulated by transforming growth factor-beta (TGF-β). Hence, lung fibroblasts are postulated to play a crucial role in aberrant lung tissue repair and remodeling, which is a main factor in lung diseases like COPD. In this study, the effect of TGF-β1 on the miRNA expression in parenchymal lung fibroblasts is investigated.

Organism:: synthetic construct; Homo sapiens

Type:: Non-coding RNA profiling by array

Platform:: GPL8786
18 Samples

Download data: CEL

Series

Accession:: GSE86185

ID:: 200086185

PubMed Full text in PMC Similar studies Analyze with GEO2R

Select item 20008618319.

mRNA expression profiling after Ago2-immunoprecipitation (IP) in unstimulated and TGF-β1-stimulated primary parenchymal lung fibroblasts of two control subjects

(Submitter supplied) To identify the target gene repertoire of miRNAs (i.e. the miRNA-targetome) of unstimulated and TGF-β1-stimulated primary parenchymal lung fibroblasts, Ago2-IP was performed followed by mRNA expression profiling.

Organism:: Homo sapiens

Type:: Other

Platform:: GPL21185
16 Samples

Download data: TXT

Series

Accession:: GSE86183

ID:: 200086183

PubMed Full text in PMC Similar studies Analyze with GEO2R

Select item 20013933420.

Gene expression analysis of fibroblasts of systemic sclerosis patients silenced for lncRNA H19X

(Submitter supplied) LncRNA H19X was silienced in dermal fibroblats of systemic sclerosis patients with antisense oligonuclotides. The hypothesis tested in the present study was that H19X is an important factor in the development of TGFb-driven fibrosis. Results provide important information about the role H19X in fibroblasts in particolar on extracellular matrix production and cell cycle regulation.

Organism:: Homo sapiens

Type:: Expression profiling by array

Platform:: GPL10558
12 Samples

Download data: IDAT

Series

Accession:: GSE139334

ID:: 200139334

PubMed Full text in PMC Similar studies Analyze with GEO2R

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