GEO DataSets

(Submitter supplied) The objectives of the present study were to analyze the changes on miRNA expression profile in swine spleen using next-generation sequencing (NGS) technology and bioinformatic analysis at 10, 25 and 50 days post inoculation (DPI) with Chinese I genotype strain of T. gondii. Compared to control group, 34, 6 and 86 differentially expressed miRNAs (DEMs) were respectively found in spleens of infected pigs at 10, 25 and 50 DPI. more...

Organism:

Sus scrofa

Type:

Non-coding RNA profiling by high throughput sequencing

Platform:

GPL19176

6 Samples

Download data: TXT

(Submitter supplied) The objectives of the present study were to analyze the changes on miRNA expression profile in porcine brain using next-generation sequencing (NGS) technology and bioinformatic analysis at 10, 25 and 50 days post inoculation (DPI) with Chinese I genotype strain of T. gondii. Compared to control group, 87, 68, and 135 differentially expressed miRNAs (DEMs) were found in the brains of infected pigs at 10, 25, and 50 DPI, respectively. more...

Organism:

Sus scrofa

Type:

Non-coding RNA profiling by high throughput sequencing

Platform:

GPL20809

6 Samples

Download data: TXT

(Submitter supplied) Purpose: Porcine alveolar macrophage was infected by T. gondii including Rh strain and Me49 strain. We want to explore the change of miRNAs after infected with T. gondii in porcine alveolar macrophages. Results: Our study generated six mi RNA expression profiles from macrophages which infect with Rh strain and Me49 and control group in different time. Compare with T. gondii-infected and uninfected with T. more...

Organism:

Sus scrofa

Type:

Non-coding RNA profiling by high throughput sequencing

Platform:

GPL19176

6 Samples

Download data: TXT

(Submitter supplied) Expression profile microarray of human foreskin fibroblast cell comparing control untreated HFF cell with HFF cell infected with ME49 strain.Study on Toxoplasma gondii infection of HFF cell LncRNAs expression, for further studies on the differential exprssion of LncRNAs in HFF cell against the infection of Toxoplasma gondii research provide the basic function.

Organism:

Homo sapiens

Type:

Expression profiling by array; Non-coding RNA profiling by array

Platform:

GPL21096

3 Samples

Download data: TXT

(Submitter supplied) Background: Histomonas meleagridis is an anaerobic, intercellular parasite that infects the Galliformes such as turkeys and chickens. In recent years, the reemergence of Histomoniasis has caused serious economic losses as drugs to treat the disease have been banned. At present, studies on H. meleagridis mainly focus on virulence, gene expression analysis, and the innate immunity of the host. However, there are no studies on differential expression of miRNAs (DEMs) in host immune and inflammatory response induced by H. more...

Organism:

Mus musculus

Type:

Non-coding RNA profiling by high throughput sequencing

Platform:

GPL17021

12 Samples

Download data: TXT

(Submitter supplied) We performed RNA-Sequencing (RNA-Seq) of primary human retinal pigment epithelial cells and the ARPE-19 cell line 24 hours following infection with GT-1 strain T. gondii tachyzoites. Gene ontology and pathway enrichment analyses of differentially expressed total RNA identified a strong immunologic transcriptomic signature. There were limited changes in the small RNA transcriptome. We used RT-qPCR for 26 immune response-related protein-coding and long non-coding transcripts in different primary epithelial cell isolates to confirm immunological activity of infected cells.

Organism:

Homo sapiens

Type:

Expression profiling by high throughput sequencing; Non-coding RNA profiling by high throughput sequencing

Platform:

GPL18573

8 Samples

Download data: XLSX

(Submitter supplied) The protozoan pathogen Toxoplasma gondii has the unique ability to develop a chronic infection in the brain of its host. The T. gondii develops cysts within the neurons that are resilient against the host immune response and current therapeutics. The bradyzoite parasites within the cyst have a sugar-protein-rich wall and a slow-replication cycle, allowing them to remain hidden from the host. This intracellular encysted lifestyle of T. more...

Organism:

Toxoplasma gondii

Type:

Expression profiling by high throughput sequencing

Platform:

GPL23377

6 Samples

Download data: GTF, TXT

(Submitter supplied) Toxoplasma gondii is an apicomplexan parasite infecting human and animals, causing huge health concerns and economic losses. However, it is unclear about the exact mechanism of T.gondii tachyzoite infected macrophage and macrophage resisted T.gondii, especially for local isolates such as TgHB1 isolated in China. Our study focused on the transcriptional difference of pig alveolar macrophages (3D4/21) infected with china isolated TgHB1 compared to TgRH and TgME49 toxoplasma gondii standard strains.

Organism:

Toxoplasma gondii; Sus scrofa

Type:

Expression profiling by high throughput sequencing

Platforms:

GPL28249 GPL22918 GPL28248

39 Samples

Download data: CSV

(Submitter supplied) Purpose:This study was aimed to profile the transcriptome changes during Toxoplasma infection with porcine alveolar macrophages. for this purpose, Porcine alveolar macrophages (PAMs,3D4/21) were infected by Toxoplasma Me49 strain in vitro. The transcriptome changes were captured by dual RNA-seq analyses. Methods: Transcriptomic profiles of infection samples (Me49 infected PAMs) and controls (Me49 and PAMs, respectively) were generated by deep sequencing, in triplicate, using Illumina HiseqPE150. more...

Organism:

Toxoplasma gondii; Sus scrofa

Type:

Expression profiling by high throughput sequencing

Platforms:

GPL28248 GPL22918 GPL28249

9 Samples

Download data: TXT

(Submitter supplied) The present study characterized comparatively the miRNA profiles of three different genotypes of T. gondii, identified genotype-shared miRNAs and strain-specific miRNAs.

Organism:

Toxoplasma gondii

Type:

Non-coding RNA profiling by high throughput sequencing

Platform:

GPL16774

5 Samples

Download data: XLSX

(Submitter supplied) The apicomplexan parasite Toxoplasma gondii infects a broad range of different cell types in avian and mammalian hosts including humans. Infection of immunocompetent hosts is mostly asymptomatic or benign, but leads to development of largely dormant bradyzoites that persist for the hosts life predominantly within neurons and muscle cells. Here we have analyzed the impact of the host cell type on the co-transcriptomes of host and parasite using high-throughput RNA sequencing. more...

Organism:

Mus musculus

Type:

Expression profiling by high throughput sequencing

Platforms:

GPL11002 GPL17021

8 Samples

Download data: XLSX

(Submitter supplied) Analysis of host cell gene expression patterns following infection of the parasite Toxoplasma gondii over a time course of 2 hours, 6 hours and 24 hours. Parasites were washed off after 2 hours to ensure that gene expression changes are truly reflective of an on-going infection, and not on-going parasite invasion.

Organism:

Homo sapiens

Type:

Expression profiling by array

Platform:

GPL10558

12 Samples

Download data: IDAT, TXT

(Submitter supplied) Analysis of mRNA-seq reveals difference in gene expression profile of immortalized bone marrow derived macrophages because of GRA15II overexpression

Organism:

Mus musculus

Type:

Expression profiling by high throughput sequencing

Platform:

GPL24247

2 Samples

Download data: TXT

(Submitter supplied) This SuperSeries is composed of the SubSeries listed below.

Organism:

Toxoplasma gondii; Mus musculus

Type:

Expression profiling by array

Platforms:

GPL25789 GPL28925 GPL29729

72 Samples

Download data: RCC

(Submitter supplied) Toxoplasma gondii is a resilient parasite that infects a multitude of warm-blooded hosts and results in a lifelong chronic infection requiring continuous responses by the host. Chronic infection is characterized by a balanced immune response and neuropathology that are driven by changes in gene expression. Previous research pertaining to these processes has been conducted in various mouse models, and much knowledge of infection-induced gene expression changes has been acquired through the use of high throughput sequencing techniques in different mouse strains and post-mortem human studies. more...

Organism:

Toxoplasma gondii; Mus musculus

Type:

Expression profiling by array

Platform:

GPL29729

24 Samples

Download data: RCC

(Submitter supplied) Toxoplasma gondii is a resilient parasite that infects a multitude of warm-blooded hosts and results in a lifelong chronic infection requiring continuous responses by the host. Chronic infection is characterized by a balanced immune response and neuropathology that are driven by changes in gene expression. Previous research pertaining to these processes has been conducted in various mouse models, and much knowledge of infection-induced gene expression changes has been acquired through the use of high throughput sequencing techniques in different mouse strains and post-mortem human studies. more...

Organism:

Mus musculus

Type:

Expression profiling by array

Platform:

GPL28925

24 Samples

Download data: RCC

(Submitter supplied) Toxoplasma gondii is a resilient parasite that infects a multitude of warm-blooded hosts and results in a lifelong chronic infection requiring continuous responses by the host. Chronic infection is characterized by a balanced immune response and neuropathology that are driven by changes in gene expression. Previous research pertaining to these processes has been conducted in various mouse models, and much knowledge of infection-induced gene expression changes has been acquired through the use of high throughput sequencing techniques in different mouse strains and post-mortem human studies. more...

Organism:

Mus musculus

Type:

Expression profiling by array

Platform:

GPL25789

24 Samples

Download data: RCC

(Submitter supplied) Toxoplasma gondii is a ubiquitous obligate intracellular parasite that infects the nucleated cells of warm-blooded animals. From within the parasitophorous vacuole in which they reside, Toxoplasma tachyzoites secrete an arsenal of effector proteins that can reprogram host gene expression to facilitate parasite survival and replication. Gaining a better understanding of how host gene expression is altered upon infection is central for understanding parasite strategies for host invasion and for developing new parasite therapies. more...

Organism:

Homo sapiens; Toxoplasma gondii RH

Type:

Expression profiling by high throughput sequencing; Other

Platform:

GPL26509

24 Samples

Download data: TAB

(Submitter supplied) We sequenced the cerebral cortex transcriptome of both male and female mice either infected with T. gondii or age-matched uninfected controls. These transcriptomes were determined along the infection timeline with early (21 and 28 days) and late (3 and 6 months) chronic represented. We obtained 5.7 billion paired-end sequences and aligned them to the Mus musculus genome. We found that a majority of the host genes with greater abundance at day 21 post-infection are still more abundant 6 months post-infection in male and female mice, although, females have far fewer genes that are significantly less abundant. more...

Organism:

Mus musculus

Type:

Expression profiling by high throughput sequencing

Platform:

GPL17021

33 Samples

Download data: TXT

(Submitter supplied) Toxoplasma gondii is a eukaryotic parasite that form latent cyst in the brain of immunocompetent individuals. The latent parasites infection of the immune privileged central nervous system is linked to most complications. With no drug currently available to eliminate the latent cysts in the brain of infected hosts, the consequences of neurons long-term infection are unknown.. It has long been known that T. more...

Organism:

Toxoplasma gondii; Rattus norvegicus

Type:

Expression profiling by high throughput sequencing

Platforms:

GPL23377 GPL18694 GPL29822

21 Samples

Download data: XLSX