GEO DataSets

(Submitter supplied) Analysis of nasopharyngeal carcinoma cell line HONE1 following knockdown of SHROOM2 gene We used microarrays to detail the global gene expression in tissues from HONE1 cells with or without SHROOM2 knockdown.

Organism:

Homo sapiens

Type:

Expression profiling by array

Platform:

GPL570

2 Samples

Download data: CEL

(Submitter supplied) Nasopharyngeal carcinoma (NPC) is a unique head and neck cancer with highly aggressive and metastatic potential, and distant metastasis is the main reason for treatment failure. The underlying molecular mechanisms of NPC metastasis remains poorly understood. Here, we have identified S100 calcium binding protein A14 (S100A14) as a functional regulator suppressed NPC metastasis via inhibiting NF-kB signaling and reversing epithelial-mesenchymal transition (EMT). more...

Organism:

Homo sapiens

Type:

Expression profiling by high throughput sequencing

Platform:

GPL16791

4 Samples

Download data: XLS

(Submitter supplied) High levels of c-Src in serum of patients with NPC was independent prognostic indicators for poor outcome of patients.

Organism:

Homo sapiens

Type:

Protein profiling by protein array

Platform:

GPL18951

8 Samples

Download data: TXT

(Submitter supplied) To explore differentially expressed genes between nasopharyngeal carcinoma (NPC) primary tumors and non-cancerous nasopharyngeal tissues, 18 NPC tissue samples versus 18 control samples were utilized to perform genome-wide expressing profiling. Consequently, 2992 genes were found to be differentially expressed in NPC tissues relative to the control (FC>2, P<0.05). Among these 2992 genes, uPA was ranked as the top one in all upregulated genes sorted by ascending order of P-value. more...

Organism:

Homo sapiens

Type:

Expression profiling by array

Platform:

GPL6480

36 Samples

Download data: TXT

(Submitter supplied) Abnormal expression of circadian genes is known to be engage in tumor initiation and progression through disrupting rhythms of cellular processes. Here, we determined a significant role of the core circadian transcription factor ARNTL2 in nasopharyngeal carcinoma metastasis. To further investigate the downstream targets of ARNTL2 in nasopharyngeal carcinoma, chromatin immunoprecipitation (ChIP) sequencing was performed in ARNTL2-overexpression HONE1 cell.

Organism:

Homo sapiens

Type:

Genome binding/occupancy profiling by high throughput sequencing

Platform:

GPL24676

2 Samples

Download data: BW, NARROWPEAK, TXT

(Submitter supplied) Abnormal expression of circadian genes is known to be engage in tumor initiation and progression through disrupting rhythms of cellular processes. Here, we determined a significant role of the core circadian gene ARNTL2 in nasopharyngeal carcinoma metastasis. To further investigate the underlying mechanism of ARNTL2 in nasopharyngeal carcinoma, High-throughput RNA sequencing was performed in ARNTL2-knockdown HONE1 cell.

Organism:

Homo sapiens

Type:

Expression profiling by high throughput sequencing

Platform:

GPL24676

6 Samples

Download data: TXT

(Submitter supplied) This SuperSeries is composed of the SubSeries listed below.

Organism:

Homo sapiens

Type:

Expression profiling by high throughput sequencing; Non-coding RNA profiling by high throughput sequencing

Platform:

GPL20301

22 Samples

Download data

(Submitter supplied) Epstein-Barr virus (EBV) is a ubiquitous human herpesvirus associated with nasopharyngeal carcinoma (NPC). EBV encodes two groups of microRNAs (miRNAs) which are divided into BamHI fragment H rightward open reading frame 1 (BHRF1) and BamHI-A rightward transcripts (BART) microRNAs. EBV miR-BARTs have been found to be involved in the development and progression of NPC. However, the role of EBV-miR-BARTs in NPC progression remains illusive. more...

Organism:

Homo sapiens

Type:

Non-coding RNA profiling by high throughput sequencing

Platform:

GPL20301

11 Samples

Download data: TSV

(Submitter supplied) To understand the genes and pathways that may potentially drive nasopharyngeal carcinoma (NPC) pathogenesis, we sought out to analyze the transcriptomic data from the disease and control samples. We used RNA-sequencing based gene expression data to identify genes differentially expressed between NPC and normal controls and then characterize the gene ontology and pathways down- or up-regulated in the disease samples.

Organism:

Homo sapiens

Type:

Expression profiling by high throughput sequencing

Platform:

GPL20301

11 Samples

Download data: TSV

(Submitter supplied) EBV encodes 44 mature miRNAs, a number of which have been found to promote carcinogenesis by targeting host genes. To determine the biological functions of the BART cluster miRNAs, we singly transfected these miRNAs into HEK293T cells. Interestingly, we found that overexpression of EBV-miR-BART6-3p in HEK293T cells dramatically altered HEK293T cell shape. To explore the mechanism of EBV-miR-BART6-3p-mediated inhibition of cell migration and invasion, we attempted to identify its downstream host cellular genes by whole genome microarray, which contains probes to all known human protein coding genes (mRNAs) and long non-coding RNA genes (lncRNAs).

Organism:

Homo sapiens

Type:

Expression profiling by array; Non-coding RNA profiling by array

Platform:

GPL17843

4 Samples

Download data: TXT

(Submitter supplied) A microarray analysis was performed on the vector and over-expressing RRM2-c2orf48 NPC cell samples(CNE2-PMSCV-Vector and CNE2-PMSCV-RRM2-c2orf48). Total RNA was extracted from 1 × 106 cells using TRIzol reagent and was further purified using a Qiagen RNeasy Mini Kit according to the manufactures’ instructions. RNA quality was assessed by formaldehyde agarose gel electrophoresis and was quantitated spectrophotometrically. more...

Organism:

Homo sapiens

Type:

Expression profiling by array

Platform:

GPL570

2 Samples

Download data: CEL

(Submitter supplied) This SuperSeries is composed of the SubSeries listed below.

Organism:

Homo sapiens

Type:

Expression profiling by high throughput sequencing; Genome binding/occupancy profiling by high throughput sequencing

Platforms:

GPL16791 GPL24676

9 Samples

Download data: BIGWIG, WIG

(Submitter supplied) Transcription factors (TFs) engage in various cellular essential processes including differentiation, growth and migration. However, the master TF involved in distant metastasis of nasopharyngeal carcinoma (NPC) remains largely unclear. Here we show that KLF5 regulates actin remodeling to enhance NPC metastasis. We analyzed the msVIPER algorithm-generated transcriptional regulatory networks and identified KLF5 as a master TF of metastatic NPC linked to poor clinical outcomes. more...

Organism:

Homo sapiens

Type:

Expression profiling by high throughput sequencing

Platform:

GPL24676

3 Samples

Download data: TXT

(Submitter supplied) Transcription factors (TFs) engage in various cellular essential processes including differentiation, growth and migration. However, the master TF involved in distant metastasis of nasopharyngeal carcinoma (NPC) remains largely unclear. Here we show that KLF5 regulates actin remodeling to enhance NPC metastasis. We analyzed the msVIPER algorithm-generated transcriptional regulatory networks and identified KLF5 as a master TF of metastatic NPC linked to poor clinical outcomes. more...

Organism:

Homo sapiens

Type:

Genome binding/occupancy profiling by high throughput sequencing

Platforms:

GPL24676 GPL16791

6 Samples

Download data: BIGWIG, WIG

(Submitter supplied) Cen3tel cells, obtained by telomerase immortalization of human fibroblasts, gradually underwent neoplastic transformation and became metastatic in immunocompromised mice. Neoplastic transformation was associated with a change in cell morphology (from fibroblastic to polygonal). Tumorigenic cells acquired a clear-cut membrane localization of adhesion molecules, a reorganization of the actin cytoskeleton, increased cell motility and invasiveness. more...

Organism:

Homo sapiens

Type:

Expression profiling by array

Platform:

GPL1708

6 Samples

Download data: TXT

(Submitter supplied) Tumor tissues contain various cell types and we focus on the stromal cell component in the tumor microenvironment to study their role and interaction. We isolated perivascular cells from two different fibrosarcoma tumor types and compared gene expression patterns.

Organism:

Mus musculus

Type:

Expression profiling by array

Platform:

GPL16570

6 Samples

Download data: CEL