Similar studies for GEO DataSets (Select 200144881) - GEO DataSets

Select item 2001448811.

Extensive SUMO modification of chromatin factors distinguishes pluripotent from somatic cells

(Submitter supplied) The post-translational modification of proteins by SUMO acts as a key gatekeeper of cell identity. In mouse embryonic fibroblast (MEFs), SUMO impedes reprogramming to pluripotency, while in embryonic stem cells (ESCs), it represses the emergence of totipotent-like cells, suggesting that SUMO targets distinct sets of substrates to preserve the somatic and pluripotent states. Using MS-based proteomics, we show here that the composition of the endogenous SUMOylomes differs dramatically between MEFs and ESCs. more...

Organism:: Mus musculus

Type:: Expression profiling by array

Platform:: GPL16570
12 Samples

Download data: CEL, CHP

Series

Accession:: GSE144881

ID:: 200144881

PubMed Full text in PMC Similar studies Analyze with GEO2R

Select item 2001158422.

SUMO safeguards somatic and pluripotent cell identities by enforcing distinct chromatin states [SUMO-ChIP-seq]

(Submitter supplied) Mouse embryonic fibroblasts (MEFs) were generated from Ubc9fl/- and Ubc9+/+ embryos (E13.5). MEFs were treated with tamoxifen for six days to cause CreERT2 activation, and induce Ubc9 floxed allele deletion. We determined the ChIP-seq profiles of SUMO-1 and SUMO-2 using chromatin of wild-type MEFs Ubc9+/+ and of the corresponding Ubc9 KO MEFs which are entirely depleted for sumoylation. The analysis revealed the nearly complete absence of genome-wide binding of SUMO-1 and SUMO-2 in Ubc9-/- MEFs, attesting for antibody specificities.

Organism:: Mus musculus

Type:: Genome binding/occupancy profiling by high throughput sequencing

Platform:: GPL17021
6 Samples

Download data: WIG

Series

Accession:: GSE115842

ID:: 200115842

PubMed Similar studies SRA Run Selector

Select item 2000990093.

SUMO safeguards somatic and pluripotent cell identities by enforcing distinct chromatin states

(Submitter supplied) Purposes: (1) compare the SUMO chromatin landscape between mouse embryonic fibroblasts (MEFs) and embryonic stem cells (ESc) (2) Decipher how hyposumoylation enhances MEF to induced pluripotent stem cells (iPSc) reprogramming (3) Decipher how hyposumoylation enhances ESc to 2C-like cells conversion. Methods : (1) ChIP-seq in MEFs and ESc for SUMO isoforms and histone marks. (2) ChIP-seq for transcription factors and histone marks at day 4 of MEF to iPSc reprogramming comparing wild type (shCtrl) and hyposumoylated cells (shUbc9). more...

Organism:: Mus musculus

Type:: Expression profiling by high throughput sequencing; Genome binding/occupancy profiling by high throughput sequencing

Platform:: GPL21103
117 Samples

Download data: BED, TDF, TXT

Series

Accession:: GSE99009

ID:: 200099009

PubMed Full text in PMC Similar studies SRA Run Selector

Select item 2001209534.

Dppa2 and Dppa4 directly regulate the Dux driven zygotic transcriptional program

(Submitter supplied) This SuperSeries is composed of the SubSeries listed below.

Organism:: Mus musculus

Type:: Expression profiling by high throughput sequencing

Platform:: GPL17021
90 Samples

Download data

Series

Accession:: GSE120953

ID:: 200120953

PubMed Full text in PMC Similar studies

Select item 2001209525.

Dppa2 and Dppa4 directly regulate the Dux driven zygotic transcriptional program [CRISPR-KO]

(Submitter supplied) The molecular regulation of zygotic genome activation (ZGA) in mammals remains poorly understood. Primed mouse embryonic stem cells contain a rare subset of “2C-like” cells that are epigenetically and transcriptionally similar to the two cell embryo and thus represent an ideal system for studying ZGA transcription regulation. Recently, the transcription factor Dux, expressed exclusively in the minor wave of ZGA, was described to activate many downstream ZGA transcripts. more...

Organism:: Mus musculus

Type:: Expression profiling by high throughput sequencing

Platform:: GPL17021
7 Samples

Download data: TXT

Series

Accession:: GSE120952

ID:: 200120952

PubMed Full text in PMC Similar studies SRA Run Selector

Select item 2001209516.

Dppa2 and Dppa4 directly regulate the Dux driven zygotic transcriptional program [siRNA]

(Submitter supplied) The molecular regulation of zygotic genome activation (ZGA) in mammals remains poorly understood. Primed mouse embryonic stem cells contain a rare subset of “2C-like” cells that are epigenetically and transcriptionally similar to the two cell embryo and thus represent an ideal system for studying ZGA transcription regulation. Recently, the transcription factor Dux, expressed exclusively in the minor wave of ZGA, was described to activate many downstream ZGA transcripts. more...

Organism:: Mus musculus

Type:: Expression profiling by high throughput sequencing

Platform:: GPL17021
9 Samples

Download data: TXT

Series

Accession:: GSE120951

ID:: 200120951

PubMed Full text in PMC Similar studies SRA Run Selector

Select item 2001209507.

Dppa2 and Dppa4 directly regulate the Dux driven zygotic transcriptional program [RNA-seq]

(Submitter supplied) The molecular regulation of zygotic genome activation (ZGA) in mammals remains poorly understood. Primed mouse embryonic stem cells contain a rare subset of “2C-like” cells that are epigenetically and transcriptionally similar to the two cell embryo and thus represent an ideal system for studying ZGA transcription regulation. Recently, the transcription factor Dux, expressed exclusively in the minor wave of ZGA, was described to activate many downstream ZGA transcripts. more...

Organism:: Mus musculus

Type:: Expression profiling by high throughput sequencing

Platform:: GPL17021
74 Samples

Download data: TXT

Series

Accession:: GSE120950

ID:: 200120950

PubMed Full text in PMC Similar studies SRA Run Selector

Select item 2001530788.

A SUMOylation-induced Satb1/Satb2 switch drives embryonic stem cell differentiation [HiC]

(Submitter supplied) The chromatin organizers Satb1 and Satb2 regulate developmental genes in a tissue- and locus-specific manner. In mouse Embryonic Stem Cells (mESCs), the Satb proteins are involved in the balance between pluripotency and differentiation by direct control of key developmental factors such as Nanog and a number of Hox genes. Despite their structural similarities, the Satb proteins regulate mESC pluripotency in opposing ways: Satb1 promotes differentiation by repressing Nanog and activating the neural genes Bcl2 and Nestin, while Satb2 supports the pluripotent state by activating Nanog and repressing Satb1. more...

Organism:: Mus musculus

Type:: Other

Platform:: GPL17021
3 Samples

Download data: H5

Series

Accession:: GSE153078

ID:: 200153078

PubMed Full text in PMC Similar studies SRA Run Selector

Select item 2001199919.

A SUMOylation-induced Satb1/Satb2 switch drives embryonic stem cell differentiation

(Submitter supplied) This SuperSeries is composed of the SubSeries listed below.

Organism:: Mus musculus

Type:: Genome binding/occupancy profiling by high throughput sequencing; Expression profiling by high throughput sequencing; Other

Platform:: GPL17021
57 Samples

Download data: BEDGRAPH, H5

Series

Accession:: GSE119991

ID:: 200119991

PubMed Full text in PMC Similar studies

Select item 20011999010.

A SUMOylation-induced Satb1/Satb2 switch drives embryonic stem cell differentiation [RNA-Seq]

(Submitter supplied) The chromatin organizers Satb1 and Satb2 regulate developmental genes in a tissue- and locus-specific manner. In mouse Embryonic Stem Cells (mESCs), the Satb proteins are involved in the balance between pluripotency and differentiation by direct control of key developmental factors such as Nanog and a number of Hox genes. Despite their structural similarities, the Satb proteins regulate mESC pluripotency in opposing ways: Satb1 promotes differentiation by repressing Nanog and activating the neural genes Bcl2 and Nestin, while Satb2 supports the pluripotent state by activating Nanog and repressing Satb1. more...

Organism:: Mus musculus

Type:: Expression profiling by high throughput sequencing

Platform:: GPL17021
22 Samples

Download data: BEDGRAPH

Series

Accession:: GSE119990

ID:: 200119990

PubMed Full text in PMC Similar studies SRA Run Selector

Select item 20011998911.

A SUMOylation-induced Satb1/Satb2 switch drives embryonic stem cell differentiation [ChIP-Seq]

(Submitter supplied) The chromatin organizers Satb1 and Satb2 regulate developmental genes in a tissue- and locus-specific manner. In mouse Embryonic Stem Cells (mESCs), the Satb proteins are involved in the balance between pluripotency and differentiation by direct control of key developmental factors such as Nanog and a number of Hox genes. Despite their structural similarities, the Satb proteins regulate mESC pluripotency in opposing ways: Satb1 promotes differentiation by repressing Nanog and activating the neural genes Bcl2 and Nestin, while Satb2 supports the pluripotent state by activating Nanog and repressing Satb1. more...

Organism:: Mus musculus

Type:: Genome binding/occupancy profiling by high throughput sequencing

Platform:: GPL17021
32 Samples

Download data: BEDGRAPH

Series

Accession:: GSE119989

ID:: 200119989

PubMed Full text in PMC Similar studies SRA Run Selector

Select item 20011717312.

Chromatin-associated factors Dppa2 and Dppa4 guide epigenetic remodeling during reprogramming to pluripotency

(Submitter supplied) This SuperSeries is composed of the SubSeries listed below.

Organism:: Mus musculus

Type:: Genome binding/occupancy profiling by high throughput sequencing; Expression profiling by high throughput sequencing

Platform:: GPL17021
73 Samples

Download data: NARROWPEAK

Series

Accession:: GSE117173

ID:: 200117173

PubMed Full text in PMC Similar studies

Select item 20011717213.

Chromatin-associated factors Dppa2 and Dppa4 guide epigenetic remodeling during reprogramming to pluripotency (RNA-seq)

(Submitter supplied) As somatic cells are converted to iPSCs, their chromatin undergoes wide-ranging rearrangements that affect the ratio of euchromatin-to-heterochromatin, DNA methylation patterns and the regulation of enhancers and promoters. The molecular machinery underlying this process remains largely unknown. Here, we show that Dppa2 and Dppa4, two thus far poorly characterized mES-specific factors, play a key role in resetting the epigenome to a pluripotent configuration. more...

Organism:: Mus musculus

Type:: Expression profiling by high throughput sequencing

Platform:: GPL17021
33 Samples

Download data: TXT

Series

Accession:: GSE117172

ID:: 200117172

PubMed Full text in PMC Similar studies SRA Run Selector

Select item 20011717114.

Chromatin-associated factors Dppa2 and Dppa4 guide epigenetic remodeling during reprogramming to pluripotency (ChIP-seq)

(Submitter supplied) As somatic cells are converted to iPSCs, their chromatin undergoes wide-ranging rearrangements that affect the ratio of euchromatin-to-heterochromatin, DNA methylation patterns and the regulation of enhancers and promoters. The molecular machinery underlying this process remains largely unknown. Here, we show that Dppa2 and Dppa4, two thus far poorly characterized mES-specific factors, play a key role in resetting the epigenome to a pluripotent configuration. more...

Organism:: Mus musculus

Type:: Genome binding/occupancy profiling by high throughput sequencing

Platform:: GPL17021
40 Samples

Download data: NARROWPEAK

Series

Accession:: GSE117171

ID:: 200117171

PubMed Full text in PMC Similar studies SRA Run Selector

Select item 20018752115.

SUMOylation of Linker Histone H1 Drives Chromatin Condensation and Restriction of Embryonic Cell Fate Identity [SMART-Seq]

(Submitter supplied) The fidelity of the early embryonic program is underlined by tight regulation of the chromatin. Yet, our understanding of how the chromatin is organized to prohibit the reversal of the developmental program remains limited. Specifically, the totipotency to pluripotency transition marks one of the most dramatic events to the chromatin, yet the nature of histone changes and regulation underlying this process are only partly characterized. more...

Organism:: Mus musculus

Type:: Expression profiling by high throughput sequencing

Platform:: GPL19057
10 Samples

Download data: TXT

Series

Accession:: GSE187521

ID:: 200187521

PubMed Similar studies SRA Run Selector

Select item 20015362016.

SUMOylation of Linker Histone H1 Drives Chromatin Condensation and Restriction of Embryonic Cell Fate Identity [RNA-seq]

(Submitter supplied) The fidelity of the early embryonic program is underlined by tight regulation of the chromatin. Yet, our understanding of how the chromatin is organized to prohibit the reversal of the developmental program remains limited. Specifically, the totipotency to pluripotency transition marks one of the most dramatic events to the chromatin, yet the nature of histone changes and regulation underlying this process are only partly characterized. more...

Organism:: Mus musculus

Type:: Expression profiling by high throughput sequencing

Platform:: GPL19057
8 Samples

Download data: TXT

Series

Accession:: GSE153620

ID:: 200153620

PubMed Similar studies SRA Run Selector

Select item 20015304117.

SUMOylation of Linker Histone H1 Drives Chromatin Condensation and Restriction of Embryonic Cell Fate Identity

(Submitter supplied) This SuperSeries is composed of the SubSeries listed below.

Organism:: Mus musculus

Type:: Genome binding/occupancy profiling by high throughput sequencing; Expression profiling by high throughput sequencing

Platform:: GPL19057
36 Samples

Download data: BED, TXT

Series

Accession:: GSE153041

ID:: 200153041

PubMed Similar studies

Select item 20015304018.

SUMOylation of Linker Histone H1 Drives Chromatin Condensation and Restriction of Embryonic Cell Fate Identity [ChIP-seq]

(Submitter supplied) The fidelity of the early embryonic program is underlined by tight regulation of the chromatin. Yet, our understanding of how the chromatin is organized to prohibit the reversal of the developmental program remains limited. Specifically, the totipotency to pluripotency transition marks one of the most dramatic events to the chromatin, yet the nature of histone changes and regulation underlying this process are only partly characterized. more...

Organism:: Mus musculus

Type:: Genome binding/occupancy profiling by high throughput sequencing

Platform:: GPL19057
10 Samples

Download data: BED

Series

Accession:: GSE153040

ID:: 200153040

PubMed Similar studies SRA Run Selector

Select item 20015303919.

SUMOylation of Linker Histone H1 Drives Chromatin Condensation and Restriction of Embryonic Cell Fate Identity [ATAC-seq]

(Submitter supplied) The fidelity of the early embryonic program is underlined by tight regulation of the chromatin. Yet, our understanding of how the chromatin is organized to prohibit the reversal of the developmental program remains limited. Specifically, the totipotency to pluripotency transition marks one of the most dramatic events to the chromatin, yet the nature of histone changes and regulation underlying this process are only partly characterized. more...