GEO DataSets

(Submitter supplied) Goal: Phenotypic evidence suggested that the transcription factor Czf1 influences the integrity of the cell wall. RNA-seq was performed to identify cell-wall related genes whose expression changes when Czf1 is artificially activated. Result: The set of genes whose expression is increased at least 4-fold is significantly enriched for genes whose GO (gene ontology) terms are "cell surface" and "cell wall", as well as several known and putative mannosyltransferases.

Organism:

Candida albicans

Type:

Expression profiling by high throughput sequencing

Platform:

GPL19036

9 Samples

Download data: TXT, XLSX

(Submitter supplied) Transcriptomic analysis of wild type and GCN5-deficient Candida albicans cells prior to and after treatment with Caspofungin 15 min and 45 min was done to determine the transcriptional changes in these strains

Organism:

Candida albicans

Type:

Expression profiling by high throughput sequencing

Platform:

GPL19036

16 Samples

Download data: TXT

(Submitter supplied) The identification of novel transcription factors associated to antifungal response may allow the discovery of fungal specific targets for new therapeutic strategies. A collection of 241 C. albicans transcriptional regulators mutants was screened for altered susceptibility to fluconazole, caspofungin, amphotericin B, and 5-fluorocytosine. Thirteen of these mutants not yet identified in their role in antifungal response were further investigated, and the function of one of them, mutant for orf19.6102 (RCA1) was characterized by transcriptome analysis. more...

Organism:

Candida albicans

Type:

Expression profiling by high throughput sequencing

Platform:

GPL15149

4 Samples

Download data: TXT

(Submitter supplied) We report the expression levels of transcripts of 5,901 genes in log-phase growing cells of the wild type CAI4 as well as the gdt1/gdt1, the pmr1/pmr1, and the gdt1/gdt1 pmr1/pmr1 mutants.We find that as compared to the wild type, there are 249, 446 and 440 differentially expressed genes (DEGs) in the gdt1/gdt1, the pmr1/pmr1 and the gdt1/gdt1 pmr1/pmr1 mutants, respectively. Deletion of CaGDT1 does not affect the transcript level of CaPMR1, and vice versa. more...

Organism:

Candida albicans

Type:

Expression profiling by high throughput sequencing

Platform:

GPL19036

4 Samples

Download data: XLS

(Submitter supplied) Skn7 is a conserved fungal heat shock factor-type transcriptional regulator. It participates in maintaining cell wall integrity and regulates the osmotic/oxidative stress response (OSR) in S. cerevisiae, where it is part of a two-component signal transduction system. Here, we comprehensively address the function of Skn7 in the human fungal pathogen Candida albicans. We provide evidence reinforcing functional divergence, with loss of the cell wall/osmotic stress-protective roles and acquisition of the ability to regulate morphogenesis on solid medium. more...

Organism:

Candida albicans

Type:

Genome binding/occupancy profiling by genome tiling array

Platform:

GPL17892

2 Samples

Download data: GPR

(Submitter supplied) Skn7 is a conserved fungal heat shock factor-type transcriptional regulator. It participates in maintaining cell wall integrity and regulates the osmotic/oxidative stress response (OSR) in S. cerevisiae, where it is part of a two-component signal transduction system. Here, we comprehensively address the function of Skn7 in the human fungal pathogen Candida albicans. We provide evidence reinforcing functional divergence, with loss of the cell wall/osmotic stress-protective roles and acquisition of the ability to regulate morphogenesis on solid medium. more...

Organism:

Candida albicans

Type:

Expression profiling by array

Platform:

GPL19932

16 Samples

Download data: GPR

(Submitter supplied) The zinc cluster proteins are a family of transcription factors that are unique to the fungal kingdom. In the pathogenic yeast Candida albicans, zinc cluster transcription factors control the expression of virulence-associated traits and play key roles in the development of antifungal drug resistance. Gain-of-function mutations in several zinc cluster transcription factors, which result in constitutive overexpression of their target genes, are a frequent cause of azole resistance in clinical C. more...

Organism:

Candida albicans

Type:

Expression profiling by array

Platform:

GPL17340

15 Samples

Download data: TXT

(Submitter supplied) Ace2 transcription factor family genes are found in many fungal genomes and are required for regulation of expression of genes involved in cell separation. We used transcriptional profiling to identify the targets of Ace2 in Candida albicans, and we show that these include several cell wall components, such as glucanases and glycosylphosphatidylinositol-anchored proteins. Expression is downregulated in ace2 deletion mutants in both yeast and hyphal cells. more...

Organism:

Candida albicans

Type:

Expression profiling by array

Platform:

GPL3727

12 Samples

Download data

(Submitter supplied) We have used DNA microarray technology to compare the expression of over 6000 genes between C. albicans WT and ACE2 knockout strains in the hyphal form. Keywords: Gene expression comparison

Organism:

Candida albicans

Type:

Expression profiling by array

Platform:

GPL3727

5 Samples

Download data

(Submitter supplied) We have used DNA microarray technology to compare the expression of over 6000 genes between C. albicans WT and ACE2 knockout strains in the yeast form. Keywords: Gene expression comparison

Organism:

Candida albicans

Type:

Expression profiling by array

Platform:

GPL3727

7 Samples

Download data

(Submitter supplied) The cell wall is essential for viability of fungi and is an effective drug target in pathogens such as Candida albicans. The contribution of posttranscriptional gene regulators to cell wall integrity in C. albicans is unknown. We show that the C. albicans Ccr4-Pop2 mRNA deadenylase, a regulator of mRNA stability and translation, is required for cell wall integrity. The ccr4/pop2 mutants display reduced wall β-glucans and sensitivity to the echinocandin caspofungin. more...

Organism:

Candida albicans

Type:

Expression profiling by array

Platform:

GPL9818

4 Samples

Download data: TXT

(Submitter supplied) The Regulation of Ace2 and Morphogenesis (RAM) pathway is an important regulatory network in the human fungal pathogen Candida albicans. The RAM pathway’s two most well-studied components, the NDR/Lats kinase Cbk1 and its putative substrate, the transcription factor Ace2, have a wide range of phenotypes and functions. It is not clear, however, which of these functions are specifically due to the phosphorylation of Ace2 by Cbk1. more...

Organism:

Candida albicans

Type:

Expression profiling by high throughput sequencing

Platform:

GPL28323

18 Samples

Download data: CSV, XLSX

(Submitter supplied) The emergence of drug-resistant fungal strains is a threat to human health. Identifying targets that increase the susceptibility of fungal pathogens to current therapies will significantly improve outcomes. Tra1 is an essential component of the SAGA and NuA4 transcriptional co-activator complexes and is linked to multiple cellular processes associated with the yeast response to antifungal drugs. This submission contains RNA sequencing data from Candida albicans strain expressing a Tra1 mutant with three arginine to glutamine mutations in the PI3K domain (tra1Q3; Berg et al., 2018) treated with or without the antifungal caspofungin. more...

Organism:

Candida albicans

Type:

Expression profiling by high throughput sequencing

Platform:

GPL15645

12 Samples

Download data: TXT

(Submitter supplied) Tra1 is an essential component of the SAGA and NuA4 transcriptional co-activator complexes and is linked to multiple cellular processes associated with the yeast response to antifungal drugs. This submission contains RNA sequencing data from Saccharomyces cerevisiae strains expressing a Tra1 mutant with three arginine to glutamine mutations in the PI3K domain (tra1Q3; Berg et al., 2018). Our goal was to identify how gene expression changes due to mutation to the PI3K domain of Tra1.

Organism:

Saccharomyces cerevisiae

Type:

Expression profiling by high throughput sequencing

Platform:

GPL13821

6 Samples

Download data: TXT

(Submitter supplied) Drug susceptible clinical isolates of Candida albicans frequently become highly tolerant to drugs during chemotherapy, with dreadful consequences on patient health. We used RNA sequencing (RNA-seq) to analyze the transcriptomes of a CDR (Candida Drug Resistance) strain and its isogenic drug sensitive counterpart. RNA-seq unveiled differential expression of 228 genes including a) genes previously identified as involved in CDR, b) genes not previously associated to the CDR phenotype, and c) novel transcripts whose function as a gene is uncharacterized. more...

Organism:

Candida albicans

Type:

Expression profiling by high throughput sequencing

Platform:

GPL15149

4 Samples

Download data: TXT

(Submitter supplied) The human pathogen Candida albicans can assume either of two distinct cell types, designated ‘‘white’’ and ‘‘opaque.’’ Each cell type is maintained for many generations; switching between them is rare and stochastic, and occurs without any known changes in the nucleotide sequence of the genome. The two cell types differ dramatically in cell shape, colony appearance, mating competence, and virulence properties. more...

Organism:

Candida albicans

Type:

Genome binding/occupancy profiling by genome tiling array

Platform:

GPL5441

7 Samples

Download data: PDF, TXT

(Submitter supplied) We employed RNA-Seq to identify ZCF8-dependent and ZCF8-indepenent transcriptional changes in Candida albicans cells treated with nigericin.

Organism:

Candida albicans

Type:

Expression profiling by high throughput sequencing

Platform:

GPL28323

8 Samples

Download data: CSV, TXT

(Submitter supplied) We employed RNA-Seq to identify targets of regulation of the transcription regualtor ZCF8 in nitrogen-starved Candida albicans cells.

Organism:

Candida albicans

Type:

Expression profiling by high throughput sequencing

Platform:

GPL28323

6 Samples

Download data: CSV, TXT

(Submitter supplied) We employed ChIP-Seq to identiy direct targets of regulation of the Candida albicans transcription regulator Zcf8p.

Organism:

Candida albicans

Type:

Genome binding/occupancy profiling by high throughput sequencing

Platform:

GPL15645

12 Samples

Download data: TXT

(Submitter supplied) The cyclic AMP-protein kinase A pathway has a central role in the biology of Candida albicans, a prominent fungal pathogen of humans. The two catalytic subunits for cyclic AMP-dependent protein kinase, Tpk1 (orf19.4892) and Tpk2 (orf19.2277), have divergent roles, and most studies indicate a more pronounced role for Tpk2. Here we dissect two Tpk1-responsive properties: adherence and cell wall integrity. more...

Organism:

Candida albicans

Type:

Expression profiling by array

Platform:

GPL9818

16 Samples

Download data: TXT