GEO DataSets

(Submitter supplied) SEC-seq human plasma cells in nanovials

Organism:

Homo sapiens

Type:

Expression profiling by high throughput sequencing

Platform:

GPL30882

10 Samples

Download data: H5

(Submitter supplied) Using secretion encoded single-cell sequencing (SEC-seq), which exploits hydrogel nanovials to capture individual cells and their secretions, we simultaneously measured the secretion of vascular endothelial growth factor A (VEGF-A) and the transcriptome for thousands of individual mesenchymal stromal cells (MSCs).

Organism:

Mus musculus; Homo sapiens

Type:

Expression profiling by high throughput sequencing; Other

Platforms:

GPL24676 GPL25526

13 Samples

Download data: TXT

(Submitter supplied) Plasma cells are key components of humoral immunity by secreting antibodies and providing protection against pathogens. These cells can be of IgM, IgA, or IgG subclass and migrate to class-specific niches. Localization and rareness of plasma cells make it challenge to define subclass-specific molecular hallmarks. Here, we describe how in-vitro differentiation of peripheral B-cells results in antibody-secreting plasma cells. more...

Organism:

Homo sapiens

Type:

Expression profiling by high throughput sequencing; Other

Platform:

GPL18573

20 Samples

Download data: TAB, TXT

(Submitter supplied) Following activation by cognate antigen, B cells undergo fine-tuning of their antigen receptors and may ultimately differentiate into antibody-secreting cells (ASCs). While antigen-specific antibodies from B cell receptor (BCR) expressing B cells can be readily cloned and sequenced following flow sorting, antigen-specific plasma cells that lack surface BCR cannot be easily profiled in a high-throughput way. more...

Organism:

Mus musculus; Homo sapiens

Type:

Expression profiling by high throughput sequencing

Platforms:

GPL24676 GPL24247

19 Samples

Download data: CSV, MTX, TSV

(Submitter supplied) We have demonstrated that thymic B cells, and more concretely CD21-CD35-, are highly heterogeneous in human neonates. To unravel the heterogeneity, we sorted and analyzed the transcriptome by single-cell RNA sequencing the CD21-CD35- B cell subset in six human donors of 4 days and 4 months old.

Organism:

Homo sapiens

Type:

Expression profiling by high throughput sequencing

Platform:

GPL24676

6 Samples

Download data: CLOUPE, CSV, MTX, TSV

(Submitter supplied) We reported two major thymic B cell subsets (CD21+CD35+ and CD21-CD35-) with different morphology, functions and transcriptome profile. For this purpose, mRNA was extracted from cord blood CD19+ B cells and thymic B cells (CD21+CD35+ and CD21-CD35-) from neonatal donors ranged between 1 day and 4 months and sequenced using Illumina HiSeq4000.

Organism:

Homo sapiens

Type:

Expression profiling by high throughput sequencing

Platform:

GPL20301

15 Samples

Download data: TXT

(Submitter supplied) To determine characterize human B cells that express IL-10 on a molecular level, we compared the global gene expression of primary CD19pos B cells secreting IL-10 or not, upon activation with anti-CD40, IL-4 and CpG for 2 days.

Organism:

Homo sapiens

Type:

Expression profiling by array

Platform:

GPL570

4 Samples

Download data: CEL, CHP