GEO DataSets

Analysis of Plasmodium falciparum chloroquine-resistant transporter (PfCRT) mutants exposed to a low dose of chloroquine (CQ) for 3 h. The clones have different degrees of CQ resistance (CQR). Results provide insight into the molecular basis of the PfCRT effect on parasite susceptibility to CQ.

Organism:

Plasmodium falciparum; Anopheles gambiae

Type:

Expression profiling by array, transformed count, 2 agent, 3 genotype/variation sets

Platform:

GPL1321

Series:

GSE10022

18 Samples

Download data: CEL

(Submitter supplied) Mutations in PfCRT confer chloroquine (CQ) resistance in P. falciparum. Point mutations in the homolog of the mammalian multidrug resistance gene (pfmdr1) can also modulate the levels of CQ response. However, parasites with the same pfcrt and pfmdr1 alleles exhibit a wide range of drug sensitivity, suggesting that additional genes contribute to levels of CQ resistance (CQR). We used 3 isogenic lines which have different drug resistance profiles corresponding to unique mutations in the pfcrt gene (106/1K76, 106/176I, and 106/76I-352K) to study changes in gene expression with and without CQ and genomic variations, i.e. more...

Organism:

Anopheles gambiae; Plasmodium falciparum

Type:

Expression profiling by array; Genome variation profiling by array

Dataset:

GDS3284

Platform:

GPL1321

24 Samples

Download data: CEL

(Submitter supplied) We compared the transcriptomic profiles between P. falciparum strains displaying mutant or wild-type pfcrt or varying in pfcrt or pfmdr1 expression levels All values were normalized using a background pool constituted of 11 transcriptome data sets that constituted a baseline for gene expression fold change and gene set enrichment. In addition to that reported in the present study, the pool included two IDC transcriptome data sets generated for Dd2 parasites pressured long-term with pulses of dihydroartemisinin (the 3b1 line) and the non-pressured parent (Lisewski et al., Cell, 2014), as well as the transcriptome data set previously reported for the 3D7, Dd2 and HB3 strains (Bozdech et al., PLos Biol 2003, Llinas et al, NAR 2006)

Organism:

Plasmodium falciparum

Type:

Expression profiling by array

Platform:

GPL7493

48 Samples

Download data: GPR, TXT

(Submitter supplied) This SuperSeries is composed of the SubSeries listed below.

Organism:

Plasmodium falciparum

Type:

Expression profiling by array

Platform:

GPL13818

27 Samples

Download data: DAT, GPR

(Submitter supplied) To identify the developmentally regulated genes, which could confound identification of PN and CQ drug responsive genes, RNA samples from drug-free synchronized cultures from ring, trophozoite, and schizont stages were individually labelled and hybridized with a pooled sample from the three stages. The data from this experiment were used to compare the developmental profile of the K1 strain with the data from other P. more...

Organism:

Plasmodium falciparum

Type:

Expression profiling by array

Platform:

GPL13818

9 Samples

Download data: GPR

(Submitter supplied) Pyronaridine (PN) and chloroquine (CQ) are structurally related anti-malarial drugs with primarily the same mode of action. However, PN is effective against several multidrug-resistant lines of Plasmodium falciparum, including CQ-resistant lines, suggestive of important operational differences between the two drugs. Synchronized trophozoite-stage cultures of P. falciparum strain K1 (CQ resistant) were exposed to 50% inhibitory concentrations (IC50) of PN and CQ, and parasites were harvested from culture after 4 and 24 hours exposure. more...

Organism:

Plasmodium falciparum

Type:

Expression profiling by array

Platform:

GPL13818

18 Samples

Download data: DAT

(Submitter supplied) To better understand the role of histone lysine acetylation in transcription in Plasmodium falciparum, we sought to attenuate the histone acetyltransferase (HAT) activity of PfGCN5 using anacardic acid (AA). We showed that AA reversibly and noncompetitively inhibited the HAT activity of recombinant PfGCN5. To a lesser extent, AA inhibited the PfGCN5 activity in parasite nuclear extracts, but did not affect the histone deacetylase activity. more...

Organism:

Plasmodium falciparum

Type:

Expression profiling by array

Platform:

GPL1858

9 Samples

Download data: TXT

(Submitter supplied) Abstract: The antimalarial activity of the antibiotic thiostrepton has long been attributed to inhibition of apicoplast protein synthesis through binding of apicoplast ribosomal RNA. However, the kinetics of parasite death upon thiostrepton treatment differ from those seen for other inhibitors of apicoplast housekeeping functions. We have analysed global changes in gene expression of the malaria parasite, Plasmodium falciparum, in an attempt to shed light on the responses of the parasite to this drug. more...

Organism:

Plasmodium falciparum; Anopheles gambiae

Type:

Expression profiling by array

Dataset:

GDS4260

Platform:

GPL1321

6 Samples

Download data: CEL

Analysis of ring stage Plasmodium falciparum at 5 % parasitaemia treated with 2 uM thiostrepton, an antibiotic with antimalarial activity. Results provide insight into molecular mechanisms underlying parasite responses to the drug.

Organism:

Plasmodium falciparum; Anopheles gambiae

Type:

Expression profiling by array, transformed count, 2 agent sets

Platform:

GPL1321

Series:

GSE28701

6 Samples

Download data: CEL

(Submitter supplied) Abstract: The mitochondrial electron transport chain is essential to Plasmodium and is the target of the antimalarial drug atovaquone. The mitochondrial genomes of Plasmodium sp. are the most reduced known, and the majority of mitochondrial proteins are encoded in the nucleus and imported into the mitochondrion post-translationally. Many organisms have signalling pathways between the mitochondria and the nucleus to regulate the expression of nuclear-encoded mitochondrially-targeted proteins, for example in response to mitochondrial dysfunction. more...

Organism:

Plasmodium falciparum; Anopheles gambiae

Type:

Expression profiling by array

Platform:

GPL1321

6 Samples

Download data: CEL