GEO DataSets

Analysis of cultured embryonic stem (ES) cells overexpressing the transcription factor STAT3. STAT3 is a downstream target of the LIF signaling cascade. LIF activation maintains ES cells in a pluripotent state. Results provide insight into the role of STAT3 in maintenance of ES cell pluripotency.

Organism:

Mus musculus

Type:

Expression profiling by array, count, 2 protocol sets

Platform:

GPL81

Series:

GSE11398

6 Samples

Download data: CEL, CHP

(Submitter supplied) To investigate the importance of STAT3 in the establishment of ES cells we have in a first step derived stable pluripotent embryonic stem cells from transgenic FVB mice expressing a conditional tamoxifen dependent STAT3-MER fusion protein. In a second step, STAT3-MER overexpressing cells were used to identify STAT3 pathway-related genes by expression profiling in order to identify new key-players involved in maintenance of pluripotency in ES cells. more...

Organism:

Mus musculus

Type:

Expression profiling by array

Datasets:

GDS3444 GDS3445 GDS3446

Platforms:

GPL82 GPL83 GPL81

18 Samples

Download data: CEL, CHP

Analysis of cultured embryonic stem (ES) cells overexpressing the transcription factor STAT3. STAT3 is a downstream target of the LIF signaling cascade. LIF activation maintains ES cells in a pluripotent state. Results provide insight into the role of STAT3 in maintenance of ES cell pluripotency.

Organism:

Mus musculus

Type:

Expression profiling by array, count, 2 protocol sets

Platform:

GPL83

Series:

GSE11398

6 Samples

Download data: CEL, CHP

Analysis of cultured embryonic stem (ES) cells overexpressing the transcription factor STAT3. STAT3 is a downstream target of the LIF signaling cascade. LIF activation maintains ES cells in a pluripotent state. Results provide insight into the role of STAT3 in maintenance of ES cell pluripotency.

Organism:

Mus musculus

Type:

Expression profiling by array, count, 2 protocol sets

Platform:

GPL82

Series:

GSE11398

6 Samples

Download data: CEL, CHP

(Submitter supplied) An NFkB target gene array (Panomics, Inc., Redwood City, CA) was performed to profile the expression pattern of 107 NFkB-regulated genes in mouse embryonic stem cells. A small scale microarray was carried out using NFkB target gene array kit (Panomics, Inc., Redwood City, CA). A long sense-strand oligonucleotide for each of the 107 human genes that have been previously shown to be regulated by NFkB signaling pathway was spotted in duplicate on the nitrocellulose membrane. more...

Organism:

Mus musculus

Type:

Expression profiling by array

Platform:

GPL6239

2 Samples

Download data: TIFF

(Submitter supplied) The pluripotency of mouse embryonic stem cells (ESC) and induced-pluripotent stem cells (iPSC) can be maintained by feeder cells, which secrete Leukemia Inhibitory Factor (LIF). We found that feeder cells provide a relatively low concentration (25 unit/ml) of LIF, which is insufficient to maintain the ESC/iPSC pluripotency in feeder free conditions. In order to identify additional factors involved in the maintenance of pluripotency, we carried out a global transcript expression profiling of mouse iPSC cultured on feeder cells and in feeder-free (LIF-treated) conditions. more...

Organism:

Mus musculus

Type:

Expression profiling by array

Platform:

GPL6105

11 Samples

Download data: TXT

(Submitter supplied) Signaling by the cytokine LIF and its downstream transcription factor, STAT3, prevents differentiation of pluripotent embryonic stem cells (ESCs) by opposing MAP kinase signaling. This contrasts with most cell types where STAT3 signaling induces differentiation. We find that STAT3 binding across the pluripotent genome is dependent upon Brg, the ATPase subunit of a specialized chromatin remodeling complex (esBAF) found in ESCs. more...

Organism:

Mus musculus

Type:

Genome binding/occupancy profiling by high throughput sequencing; Expression profiling by array

Platforms:

GPL9250 GPL1261

15 Samples

Download data: BED, CEL, TXT

(Submitter supplied) The TET family of FE(II) and 2-oxoglutarate-dependent enzymes (Tet1/2/3) promote DNA demethylation by converting 5-methylcytosine to 5-hydroxymethylcytosine (5hmC), which they further oxidize into 5-formylcytosine and 5-carboxylcytosine. Tet1 is robustly expressed in mouse embryonic stem cells (mESCs) and has been implicated in mESC maintenance. Here we demonstrate that, unlike genetic deletion, RNAi-mediated depletion of Tet1 in mESCs led to a significant reduction in 5hmC and loss of mESC identity. more...

Organism:

Mus musculus

Type:

Methylation profiling by high throughput sequencing

Platform:

GPL9250

4 Samples

Download data: BED, WIG

(Submitter supplied) The TET family of FE(II) and 2-oxoglutarate-dependent enzymes (Tet1/2/3) promote DNA demethylation by converting 5-methylcytosine to 5-hydroxymethylcytosine (5hmC), which they further oxidize into 5-formylcytosine and 5-carboxylcytosine. Tet1 is robustly expressed in mouse embryonic stem cells (mESCs) and has been implicated in mESC maintenance. Here we demonstrate that, unlike genetic deletion, RNAi-mediated depletion of Tet1 in mESCs led to a significant reduction in 5hmC and loss of mESC identity. more...

Organism:

Mus musculus

Type:

Expression profiling by array

Platform:

GPL14661

8 Samples

Download data: CEL

(Submitter supplied) This SuperSeries is composed of the SubSeries listed below.

Organism:

Mus musculus

Type:

Expression profiling by array; Methylation profiling by high throughput sequencing

Platforms:

GPL14661 GPL9250

12 Samples

Download data: BED, CEL, WIG

(Submitter supplied) Germline cell-derived pluripotent stem cells (GPSCs) are similar to embryonic stem (ES) cells in that they can proliferate intensively and differentiate into a variety of cell types, including cardiomyocytes and neurons. In this report, mouse GPSCs were induced to differentiate into hepatocytes with very high efficiency, and demonstrated, for the first time, to be functional. These hepatocytes were characterised at cellular and molecular levels. more...

Organism:

Mus musculus

Type:

Expression profiling by array

Platform:

GPL6887

42 Samples

Download data: TXT

(Submitter supplied) To identify downstream targets of Jak/Stat3 pathways without being distracted by differentiation signalings from MEK/ERK pathway, we exploited a engineered B6 cells, which stably stably expressing a chimeric receptor (GRgp-Y118F). The chimeric receptor can induce the phosphorylation of Stat3 by GCSF without activating the MEK/ERK pathway. To mimic the effect of GCSF, the chimeric B6 cells were also treated with LIF plus a selective MEK chemical inhibitor, PD0325901, to induce LIF/Jak/Stat3 but MEK/ERK pathways.

Organism:

Mus musculus

Type:

Expression profiling by array

Platform:

GPL6246

6 Samples

Download data: CEL

(Submitter supplied) The goal of this study was to identify the target genes of Stat3 involved in murine ES cell self-renewal. To achieve this goal, we used the Gs2 ES cell line expressing an inducible dominant negative mutant of Stat3 (Stat3F) in wich Y705 is mutated to phenilalanine. The Gs2 ES cells are routinely maintained in the presence of LIF 1000 U/ml and tetracycline 1µg/ml (LIF/ON). In this condition, Stat3F is not expressed and the cells are maintained in an undifferentiated phenotype. more...

Organism:

Mus musculus

Type:

Expression profiling by array

Platforms:

GPL1091 GPL1063

23 Samples

Download data

(Submitter supplied) In order to identify the genes involved in LIF/gp130 self-renewal response, we analyzed the transcriptome of Gs2 ES cells induced to differentiate upon LIF withdrawal for 16h, 24h and 48h (LIF16, LIF24 and LIF48 respectively)and compared them to that of undifferentiated ES Gs2 cells continuously maintained in the presence of LIF. Since this cell line also carry a tetracycline regulatable dominant negative form of Stat3 (Stat3F, all the experiment was done in the presence of Tetracycline (tet ON) to avoid Stat3F expression. more...

Organism:

Mus musculus

Type:

Expression profiling by array

Platform:

GPL1091

18 Samples

Download data

(Submitter supplied) Chip contains 9216 features (probes and controls): 2014 PCR products from cDNA matrix using primers specific of genes involved in important biological process (apoptosis, cell cycle, stress response). 1684 PCR products from cDNA clones from the I.M.A.G.E consortium (Research Genetics collection). 2014 PCR products from mouse cDNA clones of a substractive bank (myodistrophic versus normal muscle). 1800 PCR products from rat cDNA clones of a substractive bank (atrophied versus normal muscle). more...

Organism:

Mus musculus

5 Series

107 Samples

Download data

(Submitter supplied) Differentiated cells can be reprogrammed to an embryonic-like state by transfer of their nuclear contents into oocytes or by fusion with embryonic stem (ES) cells. Little is known about the factors that induce this reprogramming. Here we show that the combination of four factors, Oct3/4, Sox2, c-Myc, and Klf4, can generate pluripotent-like stem cells directly from mouse embryonic or adult fibroblast cultures. more...

Organism:

Mus musculus

Type:

Expression profiling by array

Platform:

GPL2881

9 Samples

Download data: TXT

(Submitter supplied) To characterize the differentiation by Sox2 KO, we performed microarray analyses of mouse ES cell line 2TS22C during the time-course being induced of Sox2 KO Keywords: development or differentiation design,time series design

Organism:

Mus musculus

Type:

Expression profiling by array

Platform:

GPL4358

12 Samples

Download data: TIFF, TXT

(Submitter supplied) We generated Nanog-/- iPSCs from both EpiSCs and pre-iPSCs. We wanted to compare the global gene expression profile of these iPSCs to their parental cells lines and to wild-type and Nanog-/- ESCs.

Organism:

Mus musculus

Type:

Expression profiling by array

Platform:

GPL6887

12 Samples

Download data: TXT

(Submitter supplied) This SuperSeries is composed of the SubSeries listed below.

Organism:

Bos taurus

Type:

Expression profiling by array

Platform:

GPL19376

12 Samples

Download data: TXT

(Submitter supplied) Genes and signaling pathways involved in pluripotency have been studied extensively in mouse and human pre-implantation embryos and embryonic stem (ES) cells. The unsuccessful attempts to generate ES cell lines from other species including cattle suggests that other genes and pathways are involved in maintaining pluripotency in these species. To investigate which genes are involved in bovine pluripotency, expression profiles were generated from morula, blastocyst, trophectoderm and inner cell mass (ICM) samples using microarray analysis. more...

Organism:

Bos taurus

Type:

Expression profiling by array

Platform:

GPL19376

4 Samples

Download data: TXT