GEO DataSets

Analysis of colon biopsies from inflammatory bowel disease (IBD) and irritable bowel syndrome (IBS) subjects. Colon biopsies also examined from 12 of these subjects (6 IBD, 6 IBS) after a 7-day diet high in red meat (300g/day). Results provide insight into molecular basis of IBD pathophysiology.

Organism:

Homo sapiens

Type:

Expression profiling by array, transformed count, 2 disease state, 2 gender, 32 individual, 2 protocol sets

Platform:

GPL6480

Series:

GSE25220

44 Samples

Download data: GPR

(Submitter supplied) Study 1: Transcriptomic profiles in colon tissue from inflammatory bowel diseases patients in relation to N-nitroso compound exposure and colorectal cancer risk Study 1: N-nitroso compounds (NOC) have been suggested to play a role in human cancer development but definitive evidence is still lacking. In this study we investigated gene expression modifications induced in human colon tissue in relation to NOC exposure to gain insight in the relevance of these compounds in human colorectal cancer (CRC) development. more...

Organism:

Homo sapiens

Type:

Expression profiling by array

Dataset:

GDS3897

Platform:

GPL6480

44 Samples

Download data: GPR

(Submitter supplied) N-nitroso compounds (NOCs) can be formed through endogenous nitrosation in the human body and are known to induce micronuclei (MN) formation in vitro. Since lymphocytic MN represent a well-validated biomarker of effect with regard to carcinogenic risk in multiple target organs, establishing a link between NOCs and MN in humans could provide evidence for a carcinogenic risk. Investigating gene expression modulations in relation to NOC exposure could provide further crucial information on underlying molecular mechanisms-of-action. more...

Organism:

Homo sapiens

Type:

Expression profiling by array

Platform:

GPL6480

30 Samples

Download data: GPR

(Submitter supplied) N-nitroso compounds (NOC) are genotoxic compounds and animal carcinogens, and may play a role in human cancer development. Since the gastro-intestinal tract is an important route of exposure, we hypothesize that NOC exposure targets genetic processes relevant in colon carcinogenesis. To investigate this, we analysed the transcriptomic effects of genotoxic concentrations of two nitrosamides, N-methyl-N'-nitro-N-nitrosoguanidine (MNNG, 1µM) and N-methyl-N-nitrosurea (MNU, 1mM), and four nitrosamines, N-nitrosodiethylamine (NDEA, 50mM), N-nitrosodimethylamine (NDMA, 100mM), N-nitrosopiperidine (NPIP, 40mM), and N-nitrosopyrrolidine (NPYR, 100mM), in the human colon carcinoma cell line Caco-2. more...

Organism:

Homo sapiens

Type:

Expression profiling by array

Platform:

GPL6480

24 Samples

Download data: TXT

(Submitter supplied) N-nitroso compounds (NOC) may be implicated in human colon carcinogenesis, but the toxicological mechanisms involved have not been elucidated. Since it was previously demonstrated that nitrosamines and nitrosamides, representing two classes of NOC, induce distinct gene expression effects in colon cells that are particularly related to oxidative stress, we hypothesized that different radical mechanisms are involved. more...

Organism:

Homo sapiens

Type:

Expression profiling by array

Platform:

GPL4133

36 Samples

Download data: GPR

(Submitter supplied) The series was designed to obtain gene expression patterns of TNF-alpha stimulated CaCo-2 cells. Keywords: TNF-alpha stimulation

Organism:

Homo sapiens

Type:

Expression profiling by array

Platform:

GPL284

33 Samples

Download data

(Submitter supplied) The series was designed to obtain gene expression patterns of TNF-alpha stimulated THP-1 cells. Keywords: TNF-alpha stimulation

Organism:

Homo sapiens

Type:

Expression profiling by array

Platform:

GPL284

15 Samples

Download data

(Submitter supplied) Damage of the intestinal epithelial barrier by xenobiotics or reactive oxygen species and a dysregulated immune response are both factors involved in the pathogenesis of inflammatory bowel diseases (IBD). Curcumin and rutin are polyphenolic compounds known to have anti-oxidant and anti-inflammatory activities, but their mechanism(s) of action are yet to be fully elucidated. Mdr1a-/- mice spontaneously develop intestinal inflammation, predominantly in the colon, with pathology similar to IBD, so this mouse model is relevant for studying diet-gene interactions and potential effects of foods on remission or development of IBD. more...

Organism:

Mus musculus

Type:

Expression profiling by array

Platform:

GPL2872

8 Samples

Download data: GPR

(Submitter supplied) Background and Aims: Gene expression analysis of colon biopsies using high-density oligonucleotide microarray can contribute to the understanding of local pathophysiological alterations and to functional classification of precancerous adenoma, different stage colorectal carcinomas (CRC) and inflammatory bowel diseases (IBD). Results: Significant overexpression of collagen IV, lipocalin-2, caveolin-1, calumenin genes, and significant dowregulation of aquaporin-8, amnionless homolog, prostaglandin D2 receptor genes were detected in CRC patients compared to normal. more...

Organism:

Homo sapiens

Type:

Expression profiling by array

Platform:

GPL570

53 Samples

Download data: CEL