GEO DataSets

Human embryonic kidney cells HEK293 transfected with the Ewing family tumor (EFT)-specific EWS-FLI1 fusion protein to investigate the histogenetic origin of EFTs. The observed EFT-specific gene expression profile supports the concept of a neural crest and endothelial origin of EFT stem cells.

Organism:

Homo sapiens

Type:

Expression profiling by array, count, 3 cell line sets

Platform:

GPL96

Series:

GSE1822

9 Samples

Download data

(Submitter supplied) Comparison of gene expression profiles between neuroblastoma samples and Ewing family tumor samples. RNA from native tumor samples was processed for DNA-microarray analysis using Affymetrix HG-U133A microarrays. Primary image analysis was performed using MAS 5.0 and data were scaled to an target intesity of 500. Keywords: ordered

Organism:

Homo sapiens

Type:

Expression profiling by array

Dataset:

GDS1713

Platform:

GPL96

10 Samples

Download data: CEL, TXT

(Submitter supplied) Gene expression analysis of cell lines initially established as neuroblastoma cell lines. Cells were harvested and processed for DNA-microarray analysis using Affymetrix HG-U133A microarrays. Primary image analysis was performed using MAS 5.0 and data were scaled to an target intesity of 500. Keywords: other

Organism:

Homo sapiens

Type:

Expression profiling by array

Dataset:

GDS1548

Platform:

GPL96

4 Samples

Download data: CEL

(Submitter supplied) Response of A673-cells to transfection with interleukin 2 (IL-2). Ewing family tumor (EFT) cell line A673 was transfected with an expression vector for IL-2 (cell line SBVGA1) or empty control vector (cell line SBVGA3). Cells were harvested at 3 different time points and processed for DNA-microarray analysis using Affymetrix HG-U95A microarrays. Primary image analysis was performed using MAS 5.0. In addition, primary analysis was performed with MAS 4.0 and data were scaled to an target intesity of 200. more...

Organism:

Homo sapiens

Type:

Expression profiling by array

Dataset:

GDS918

Platform:

GPL91

9 Samples

Download data

(Submitter supplied) Response of HEK293-cells after transfection with EWS-FLI1. HEK293 cells were transfected with the expression vector pIRES2-EGFP containing type I EWS-FLI1 or empty control vector. For transient transfection standard DEAE dextran method was used and RNA was isolated 48h post transfection. For stable transfection cells were transfected using FuGENE 6 (Roche, Mannheim, Germany) and cells were selected with 400 ug/mL G418. more...

Organism:

Homo sapiens

Type:

Expression profiling by array

Dataset:

GDS916

Platform:

GPL96

9 Samples

Download data

Analysis of Ewing family tumor (EFT) and neuroblastoma (NB) samples. Distinguishing EFTs from other small round blue cell tumors (SRBCTs), such as NBs, by histologic methods is often difficult. Results identify EF-associated genes that enable molecular discrimination between EFTs and other SRBCTs.

Organism:

Homo sapiens

Type:

Expression profiling by array, count, 2 disease state sets

Platform:

GPL96

Series:

GSE1825

10 Samples

Download data: CEL

Analysis of four cell lines initially established as neuroblastomas. Results indicate that the SK-N-MC cell line expresses all of the Ewing Family Tumor (EFT) signature genes. EFTs are small round blue cell tumors that show features of neuroectodermal differentiation.

Organism:

Homo sapiens

Type:

Expression profiling by array, count, 4 cell line, 2 disease state sets

Platform:

GPL96

Series:

GSE1824

4 Samples

Download data: CEL

Analysis of Ewing family tumor (EFT) cell line A673 response to interleukin-2 (IL-2) transfection to assess possible undesirable effects (e.g. tumor stimulation) of in vitro manipulation. Results impact the potential use of IL-2 transfection as an in vivo immunotherapy for EFT patients.

Organism:

Homo sapiens

Type:

Expression profiling by array, count, 3 cell line, 3 isolate sets

Platform:

GPL91

Series:

GSE1823

9 Samples

Download data

(Submitter supplied) The cellular origin of Ewing tumor (ET), a tumor of bone or soft tissues characterized by specific fusions between EWS and ETS genes, is highly debated. Through gene expression analysis comparing ETs with a variety of normal tissues, we show that the profiles of different EWS-FLI1-silenced Ewing cell lines converge toward that of mesenchymal stem cells (MSC). Moreover, upon EWS-FLI1 silencing, two different Ewing cell lines can differentiate along the adipogenic lineage when incubated in appropriate differentiation cocktails. more...

Organism:

Homo sapiens

Type:

Expression profiling by array

Platform:

GPL96

39 Samples

Download data: CEL

(Submitter supplied) The molecular hallmark of the Ewing family of tumors is the presence of balanced chromosomal translocations leading to the formation of chimerical transcription factors (i.e. EWS/FLI1) that play a pivotal role in the pathogenesis of Ewing tumors by deregulating gene expression. We have recently demonstrated that DAX1 (NR0B1), an orphan nuclear receptor which was not previously implicated in cancer, is induced by the EWS/FLI1 oncoprotein and is highly expressed in Ewing tumors, suggesting that DAX1 is a biologically relevant target of EWS/FLI1-mediated oncogenesis. more...

Organism:

Homo sapiens

Type:

Expression profiling by array

Platform:

GPL2895

12 Samples

Download data: TXT