GEO DataSets

(Submitter supplied) The cell cycle-regulated DNA methyltransferase CcrM is conserved in most Alphaproteobacteria, but its role in bacteria with complex or multicentric genomes remains unexplored. Here, we compare the methylome, the transcriptome and the phenotypes of wild-type and CcrM-depleted Agrobacterium tumefaciens cells with a dicentric genome with two essential replication origins. We find that DNA methylation has a pleiotropic impact on motility, biofilm formation and viability. more...

Organism:

Agrobacterium tumefaciens

Type:

Expression profiling by high throughput sequencing

Platform:

GPL34084

9 Samples

Download data: TXT

(Submitter supplied) Many pathogens and symbionts contain multipartite genomes, but how they are stably maintained is poorly understood. The plant pathogen, Agrobacterium tumefaciens, contains four replicons. Recent work indicates that their replication origins cluster at cell poles in a manner that depends on their ParB-family centromeric proteins. Here, we provide evidence that centromeric clustering is mediated by interactions between these centromeric proteins. more...

Organism:

Bacillus subtilis PY79; Agrobacterium tumefaciens

Type:

Genome binding/occupancy profiling by high throughput sequencing; Other

Platforms:

GPL28115 GPL30555

29 Samples

Download data: CSV, TXT, ZIP

(Submitter supplied) Bacterial species from diverse phyla contain multiple replicons, yet how these multipartite genomes are organized and segregated during the cell cycle remains poorly understood. Agrobacterium tumefaciens has a 2.8 Mb circular chromosome (Ch1), a 2.1 Mb linear chromosome (Ch2), and two large plasmids (pAt and pTi). We used this alpha proteobacterium as a model to investigate the global organization and temporal segregation of a multipartite genome. more...

Organism:

Agrobacterium tumefaciens

Type:

Genome binding/occupancy profiling by high throughput sequencing; Other

Platforms:

GPL25733 GPL30555

42 Samples

Download data: CSV, TXT

(Submitter supplied) Purpose: The goals of this study is to compare the reponse of Agrobacterium tumefaciens C58 in the presence of GHB and GABA to delineated the key-genes associated to the response of these metabolites in A. tumefaciens C58.

Organism:

Agrobacterium tumefaciens

Type:

Expression profiling by high throughput sequencing

Platform:

GPL25733

12 Samples

Download data: TXT

(Submitter supplied) Purpose: The goals of this study is to compare the reponse of Agrobacterium tumefaciens C58 in the presence and absence of the two opines nopaline and agrocinopine (more precisely agrocinopine A) to delineated the key-genes associated to opines-response in A. tumefaciens C58.

Organism:

Agrobacterium tumefaciens

Type:

Expression profiling by high throughput sequencing

Platform:

GPL24197

6 Samples

Download data: TXT

(Submitter supplied) A conserved regulatory pathway directs developmental transitions and asymmetries in Agrobacterium tumefaciens. Core components of this coordination of division and development (CDD) pathway include two integrated phosphorelays. One of these phosphorelays includes at least four histidine sensor kinase homologues, DivJ, PleC, PdhS1, and PdhS2, and at least two response regulators, DivK and PleD. Previously we demonstrated that PdhS2 reciprocally regulates biofilm formation and swimming motility. more...

Organism:

Agrobacterium tumefaciens

Type:

Expression profiling by array

Platform:

GPL18993

3 Samples

Download data: GPR, PDF, XLSX

(Submitter supplied) The plant pathogen Agrobacterium tumefaciens attaches to and forms biofilms on both biotic and abiotic surfaces. The transition between free-living, planktonic A. tumefaciens and multicellular biofilms is regulated by several well-defined environmental and nutritional inputs, including pH, oxygen tension, and phosphate concentration. In many bacterial species limiting iron levels inhibit attachment and biofilm formation. more...

Organism:

Agrobacterium tumefaciens

Type:

Expression profiling by array

Platform:

GPL18993

4 Samples

Download data: GPR, PDF, XLSX

(Submitter supplied) The plant pathogen Agrobacterium tumefaciens attaches to and forms biofilms on both biotic and abiotic surfaces. The transition between free-living, planktonic A. tumefaciens and multicellular biofilms is regulated by several well-defined environmental and nutritional inputs, including pH, oxygen tension, and phosphate concentration. In many bacterial species limiting iron levels inhibit attachment and biofilm formation. more...

Organism:

Agrobacterium tumefaciens

Type:

Expression profiling by array

Platform:

GPL18993

4 Samples

Download data: GPR, XLS

(Submitter supplied) Co-immunoprecipitation with endogenous Hfq3xFlag in exponential, stationary, non-induced and virulence induced conditions, followed by RNA-sequencing, revealed 1697 mRNAs and 208 ncRNAs associated with Hfq. We identified 56 new ncRNAs and validated 3 Hfq-dependent trans sRNAs on by Northern blot analysis. Interestingly, 55% of the ncRNAs were encoded antisense to a protein coding sequence. Abundance of 4 asRNAs and their corresponding target mRNAs was altered upon hfq, indicating a substantial influence of Hfq on asRNA-target interactions.

Organism:

Agrobacterium tumefaciens

Type:

Expression profiling by high throughput sequencing

Platform:

GPL18897

8 Samples

Download data: WIG

(Submitter supplied) Many bacteria colonize surfaces and transition to a sessile mode of growth. The plant pathogen Agrobacterium tumefaciens produces a unipolar polysaccharide (UPP) adhesin at single cell poles that contact surfaces. Here we report that elevated levels of the intracellular signal cyclic diguanosine monophosphate (c-di-GMP) lead to surface-contact-independent UPP production and a red colony phenotype due to production of UPP and the exopolysaccharide cellulose, when A. more...

Organism:

Agrobacterium tumefaciens

Type:

Expression profiling by array

Platform:

GPL18993

3 Samples

Download data: GPR, TXT

(Submitter supplied) This SuperSeries is composed of the SubSeries listed below.

Organism:

Agrobacterium tumefaciens

Type:

Expression profiling by array

Platform:

GPL18993

7 Samples

Download data: GPR

(Submitter supplied) Agrobacterium tumefaciens is a facultative plant pathogen and the causative agent of crown gall disease. The initial stage of infection involves attachment to plant tissues and, subsequently, biofilms may form at these sites. This study focuses on the periplasmic ExoR regulator, which was identified based on the severe biofilm deficiency of A. tumefaciens exoR mutants. Genome-wide expression analysis was performed to elucidate the complete ExoR regulon. more...

Organism:

Agrobacterium tumefaciens

Type:

Expression profiling by array

Platform:

GPL18993

3 Samples

Download data: GPR, TXT, XLS

(Submitter supplied) Agrobacterium tumefaciens is a facultative plant pathogen and the causative agent of crown gall disease. The initial stage of infection involves attachment to plant tissues and, subsequently, biofilms may form at these sites. This study focuses on the periplasmic ExoR regulator, which was identified based on the severe biofilm deficiency of A. tumefaciens exoR mutants. Genome-wide expression analysis was performed to elucidate the complete ExoR regulon. more...

Organism:

Agrobacterium tumefaciens

Type:

Expression profiling by array

Platform:

GPL18993

4 Samples

Download data: GPR, TXT, XLS

(Submitter supplied) AMADID # 012657 (11K/features/array)(001,002), Single Customer Protocol: See manufacturer's website

Organism:

Agrobacterium tumefaciens

7 Series

21 Samples

Download data: GAL