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Items: 1 to 20 of 2981

1.

miRNAs regulated systemically by nitrogen availability in shoots and roots of Medicago truncatula

(Submitter supplied) Using a dedicated split-root approach, we identified miRNAs regulated systemically by nitrogen availability in both shoots and roots of the Medicago truncatula model legume, depending on the CRA2 pathway, highlighting the phosphate-related miR399.
Organism:
Medicago truncatula
Type:
Non-coding RNA profiling by high throughput sequencing
Platform:
GPL30272
24 Samples
Download data: TSV
Series
Accession:
GSE247405
ID:
200247405
2.

The miR390/TAS3/ARF module governs the expression of an LBD transcription factor to regulate nodulation and root architecture in Medicago truncatula

(Submitter supplied) The microRNA390 targets the TAS3 transcript leading to the production of trans-acting small interference RNAs that subsequently post-transcriptionally repress the mRNAs encoding the Auxin Response Factors (ARF)2, ARF3, and ARF4. This module has been linked to the development of lateral organs in both aerial and underground tissues. Previously, we have shown that the miR390/TAS3/ARFs module mediates the control of lateral roots and symbiotic nodules in legumes. more...
Organism:
Medicago truncatula
Type:
Expression profiling by high throughput sequencing
Platform:
GPL33966
8 Samples
Download data: TXT
Series
Accession:
GSE248872
ID:
200248872
3.

Gene-edited Mtsoc1 triple mutant Medicago plants do not flower

(Submitter supplied) Optimised flowering time is an important trait ensuring successful plant adaptation and crop productivity. SOC1-like genes encode MADS transcription factors known to play important roles in flowering control in many plants. This includes the best characterised eudicot model Arabidopsis thaliana (Arabidopsis) where SOC1 promotes flowering and functions as a floral integrator gene integrating signals from different flowering time regulatory pathways. more...
Organism:
Medicago truncatula
Type:
Expression profiling by high throughput sequencing
Platform:
GPL30272
15 Samples
Download data: XLSX
Series
Accession:
GSE247931
ID:
200247931
4.

The phosphate starvation response regulator PHR2 antagonizes arbuscule maintenance in Medicago

(Submitter supplied) • Phosphate starvation response (PHR) transcription factors play essential roles in regulating phosphate uptake in plants through binding to the P1BS cis-element in the promoter of phosphate starvation response genes. Recently, PHRs were also shown to positively regulate arbuscular mycorrhizal colonization in rice and lotus by controlling the expression of a large set of symbiotic genes. However, their role in arbuscule development has remained unclear. more...
Organism:
Medicago truncatula
Type:
Expression profiling by high throughput sequencing
Platform:
GPL30272
6 Samples
Download data: TXT
Series
Accession:
GSE252717
ID:
200252717
5.

The single-cell transcriptome program of nodule development cellular lineages in Medicago truncatula

(Submitter supplied) Medicago truncatula engages in root nodule symbiosis by developing a de novo plant organ (known as nodule) in its roots in response to the infection by rhizobia. These nodules are de novo plant organs that provide an optimal environment for the rhizobia to fix nitrogen in exchange for photosynthates. The establishment of root nodule symbioses (RNS) requires the coordination of two distinct processes: bacterial infection and nodule organogenesis. more...
Organism:
Medicago truncatula
Type:
Expression profiling by high throughput sequencing
Platform:
GPL30272
8 Samples
Download data: MTX, TSV
Series
Accession:
GSE224539
ID:
200224539
6.

Spatial Co-transcriptomics Reveals Discrete Stages of the Arbuscular Mycorrhizal Symbiosis

(Submitter supplied) This SuperSeries is composed of the SubSeries listed below.
Organism:
Medicago truncatula
Type:
Expression profiling by high throughput sequencing
Platform:
GPL30272
27 Samples
Download data: CSV, JPG, JSON, MTX, PNG, TSV
Series
Accession:
GSE240107
ID:
200240107
7.

Spatial Co-transcriptomics Reveals Discrete Stages of the Arbuscular Mycorrhizal Symbiosis II

(Submitter supplied) The symbiotic interaction of plants with arbuscular mycorrhizal fungi (AM fungi) is ancient and widespread. Plants provide AM fungi with carbon in exchange for nutrients and water, making this interaction a prime target for crop improvement. However, plant-fungal interactions are restricted to a small subset of root cells, precluding the application of most conventional functional genomic techniques to study the molecular bases of these interactions. more...
Organism:
Medicago truncatula
Type:
Expression profiling by high throughput sequencing
Platform:
GPL30272
20 Samples
Download data: CSV, JPG, JSON, MTX, PNG, RDS, TSV
Series
Accession:
GSE240102
ID:
200240102
8.

Spatial Co-transcriptomics Reveals Discrete Stages of the Arbuscular Mycorrhizal Symbiosis I

(Submitter supplied) The symbiotic interaction of plants with arbuscular mycorrhizal fungi (AM fungi) is ancient and widespread. Plants provide AM fungi with carbon in exchange for nutrients and water, making this interaction a prime target for crop improvement. However, plant-fungal interactions are restricted to a small subset of root cells, precluding the application of most conventional functional genomic techniques to study the molecular bases of these interactions. more...
Organism:
Medicago truncatula
Type:
Expression profiling by high throughput sequencing
Platform:
GPL30272
7 Samples
Download data: CSV, MTX, RDS, TSV
Series
Accession:
GSE240098
ID:
200240098
9.

Identifying the regulon of the Sinorhizobium meliloti JspA protease

(Submitter supplied) Sinorhizobium meliloti establishes symbiotic relationship with compatible leguminous plants by inducing root nodule formation, colonizing such nodules, and fixing molecular nitrogen for the host in exchange for carbon compounds. This mutualistic process requires complex communication and tight regulation to allow yet constrain infection to specific tissues. Production of succinoglycan, or exopolysaccharide-I (EPS-I), enables S. more...
Organism:
Medicago truncatula; Sinorhizobium meliloti
Type:
Expression profiling by array
Platform:
GPL9757
9 Samples
Download data: CEL, XLSX
Series
Accession:
GSE155833
ID:
200155833
10.

The change of gene expression between late- and early stage lin-5 nodules

(Submitter supplied) To investigate the function LIN in the regulation of the symbiosome formation, we established the gene expression level between 4 wpi and 2 wpi lin-5 nodule samples
Organism:
Medicago truncatula
Type:
Expression profiling by high throughput sequencing
Platform:
GPL30272
6 Samples
Download data: CSV
Series
Accession:
GSE227642
ID:
200227642
11.

Genome-wide distribution of H3K4me3 in Medicago truncatula wild type R108 and Mting2-1 mutant.

(Submitter supplied) Medicago truncatula (Medicago) flowering is promoted by winter cold (vernalization) followed by long day photoperiods (VLD). By analysing a Tnt1 insertion mutant and 17 CRISPR-Cas9 gene-edited lines with delayed flowering, we identified INHIBITOR OF GROWTH (ING) 2 (MtING2) encoding a plant homeodomain (PHD) zinc finger and predicted H3K4me2/3 epigenome reader in Medicago. There are two ING genes in most plants, but their physiological role has not been described. more...
Organism:
Medicago truncatula
Type:
Genome binding/occupancy profiling by high throughput sequencing
Platform:
GPL30272
6 Samples
Download data: BW
Series
Accession:
GSE189617
ID:
200189617
12.

Global differential gene expression between Medicago truncatula wild type R108 and Mting2-1 mutant.

(Submitter supplied) Medicago truncatula (Medicago) flowering is promoted by winter cold (vernalization) followed by long day photoperiods (VLD). By analysing a Tnt1 insertion mutant and 17 CRISPR-Cas9 gene-edited lines with delayed flowering, we identified INHIBITOR OF GROWTH (ING) 2 (MtING2) encoding a plant homeodomain (PHD) zinc finger and predicted H3K4me2/3 epigenome reader in Medicago. There are two ING genes in most plants, but their physiological role has not been described. more...
Organism:
Medicago truncatula
Type:
Expression profiling by high throughput sequencing
Platform:
GPL30272
12 Samples
Download data: XLSX
Series
Accession:
GSE189301
ID:
200189301
13.

Gene expression profile at single cell level of Medicago truncatula roots in response to rhizobia infection

(Submitter supplied) The nuclei of Medicago truncatula root seedlings exposed to rhizobia were isolated at single-cell resolution with single-nuclei RNA-seq applying 10x Genomics technology.
Organism:
Medicago truncatula
Type:
Expression profiling by high throughput sequencing
Platform:
GPL30272
6 Samples
Download data: RDS, TXT
Series
Accession:
GSE210881
ID:
200210881
14.

Nutrient regulation of lipochitooligosaccharide recognition in plants via NSP1 and NSP2

(Submitter supplied) Many plants associate with arbuscular mycorrhizal fungi for nutrient acquisition, while legumes also associate with nitrogen-fixing rhizobial bacteria. Both associations rely on symbiosis signaling and here we show that cereals can perceive lipochitooligosaccharides (LCOs) for activation of symbiosis signaling, surprisingly including Nod factors produced by nitrogen-fixing bacteria. However, legumes show stringent perception of specifically decorated LCOs, that is absent in cereals. more...
Organism:
Hordeum vulgare; Medicago truncatula
Type:
Expression profiling by high throughput sequencing
Platforms:
GPL30272 GPL29652
90 Samples
Download data: TSV
Series
Accession:
GSE214698
ID:
200214698
15.

RNAseq on 1mm and 5 mm germinating seeds with/without PEG treatment from dissected radicles and cotyledons

(Submitter supplied) RNAseq from Medicago truncatula early post germination (1 and 5mm radicle seeds) from dissected radicles and cotyledons with and without PEG treatment to identify genes induced during the reinduction of the desiccation tolerance at early germination. Dessication tolerant tissues were radicles from 1mm radicle seeds treated with PEG: R1P, and cotyledons from 1- and 5-mm radicle seeds treated or not with PEG: C1, C5, C1P, C5P and desiccation sensitive tissues were radicles from 1mm radicle seeds (R1) and 5mm radicle seeds (R5).
Organism:
Medicago truncatula
Type:
Expression profiling by high throughput sequencing
Platform:
GPL26728
16 Samples
Download data: TXT
Series
Accession:
GSE214468
ID:
200214468
16.

DNA sequencing of Medicago truncatula from ATAC-seq experiment - roots of germinated seeds at 1mm, 1mm+PEG, 5mm

(Submitter supplied) We analysed chromatin changes in Medicago (A17) between radicles from germinated tolreant to desiccation (R1P) or icompared to intolerant to desiccation (R1 and R5). Roots at 1mm were dried for 72hours and are desiccation sensitive, roots at 1mm plus incubation in PEG 8000 at -1.7Mpa for 72h at 10°C then dried for 72hours are desiccation tolerant and roots at 5mm dried for 72h are desiccation sensitive
Organism:
Medicago truncatula
Type:
Genome binding/occupancy profiling by high throughput sequencing
Platform:
GPL30653
8 Samples
Download data: BW
Series
Accession:
GSE214221
ID:
200214221
17.

DNA sequencing of Medicago truncatula from ChIP-seq experiment - roots of germinated seeds at 1mm, 1mm+PEG, 5mm (after immunoprecipitation of H3k27me3)

(Submitter supplied) We performed Chromatine ImmunoPrecipitation of the Histone H3K27me3 mark in 1- and 5mm Medicago (A17) radicles which were desiccation sensitive (R1 and R5) and desiccation tolerant (R1P). Roots at 1mm were dried for 72hs and are desiccation sensitive, roots at 1mm plus incubation in PEG 8000 at -1.7Mpa for 72h at 10°C then dried for 72hs are desiccation tolerant and roots at 5mm dried for 72h are desication sensitive
Organism:
Medicago truncatula
Type:
Genome binding/occupancy profiling by high throughput sequencing
Platform:
GPL30653
12 Samples
Download data: BW
Series
Accession:
GSE214220
ID:
200214220
18.

DNA sequencing of Medicago truncatula from ChIP-seq experiment - roots of germinated seeds at 1mm, 1mm+PEG, 5mm (after immunoprecipitation of H2AK119Ub)

(Submitter supplied) We performed Chromatine ImmunoPrecipitation of the Histone H2AK119Ub mark in 1- and 5mm Medicago (A17) radicles which were desiccation sensitive (R1 and R5) and desiccation tolerant (R1P). Roots at 1mm were dried for 72hs and are desiccation sensitive, roots at 1mm plus incubation in PEG 8000 at -1.7Mpa for 72h at 10°C then dried for 72hs are desiccation tolerant and roots at 5mm dried for 72h are desication sensitive
Organism:
Medicago truncatula
Type:
Genome binding/occupancy profiling by high throughput sequencing
Platform:
GPL30653
12 Samples
Download data: BW
Series
Accession:
GSE214218
ID:
200214218
19.

Reprogramming of root cells during nitrogen-fixing symbiosis entails dynamic polysome association of coding and non-coding RNAs

(Submitter supplied) GMUCT 2.0 data were analyzed to globally identify 5’ ends differentially accumulated in SKI3 RNAi roots as compared with GUS RNAi, as well as those that change in abundance in response to rhizobia SUPERKILLER3 (*SKI3),* a member of SKI complex that guides RNAs to the 3 to 5' exoribonuclease of the exosome complex. In the absence of a functional SKI complex to thread transcripts to the exosome, the bulk of the mRNAs that accumulate in the cytoplasm are diverted to the 5 -to-3 XRN4 pathway for degradation. more...
Organism:
Medicago truncatula
Type:
Expression profiling by high throughput sequencing
Platform:
GPL21643
12 Samples
Download data: XLSX
Series
Accession:
GSE135920
ID:
200135920
20.

Transcriptome analysis of developing seeds from Mtabi4 Tnt1 insertion mutants

(Submitter supplied) ABI4 (MtrunA17_Chr5g0437371) is an AP2 transcription factor that is upregulated during seed maturation. To identify genes that are regulated by this transcription factor in the model legume Medicago truncatula, Tnt1 insertion mutants were identified and RNA sequencing was performed on two abi4 mutants and the wild type seeds at 13 DAP, 24 DAP and mature seeds.
Organism:
Medicago truncatula
Type:
Expression profiling by high throughput sequencing
Platform:
GPL26728
21 Samples
Download data: TXT
Series
Accession:
GSE212927
ID:
200212927
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