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Platform GPL280 Query DataSets for GPL280
Status Public on Mar 29, 2003
Title NIAID - Mtaa
Technology type spotted oligonucleotide
Distribution non-commercial
Organism Mycobacterium tuberculosis
 
Description Printing by NIAID / Microarray Research Facility
Surface type: non-adsorptive, polylysine-coated glass
Attachment: covalent, (UV-treated polylysine)
Array description: The custom microarrays are manufactured by the Microarray Research Facility of the National Institute of Allergy and Infectious Diseases (NIH/NIAID). Microarrays are printed with an Omnigrid robotic arrayer by GeneMachines (http://www.genemachines.com) that is fitted with a printhead and 16 SMP5 print tips from Telechem (http://www.arrayit.com). The M. tuberculosis microarray (Mtaa) probe set is based on 70mer oligonucleotides from Qiagen's Tuberculosis Genome Oligo Set V1 (http://oligos.qiagen.com/arrays/omad.php). Oligonucleotide probes are distributed into 384-well polypropylene plates (Genetix, 384 micro array) and dissolved in 4XSSC print buffer at a normalized 25 micromolar concentration. The Mtaa microarray spot densities are 250 microns(center-to-center). The microarrays are printed on poly-L-lysine coated slides and post-processed according to the procedures on the Stanford website: http://cmgm.stanford.edu/pbrown/protocols/index.html
 
Web link http://madb.niaid.nih.gov/gal_files/gal_niaid.shtml
Submission date Mar 28, 2003
Last update date Dec 28, 2005
Contact name Michael Wilson
E-mail(s) mwilson@ambion.com
Phone 301-496-4287
URL http://madb.niaid.nih.gov/
Organization name National Institute of Allergy and Infectious Diseases
Department Division of Intramural Research
Lab Research Technologies Branch / Microarray Research Facility
Street address 50 South Drive, Room 5509
City Bethesda
State/province MD
ZIP/Postal code 20892
Country USA
 
Samples (28) GSM5272, GSM5273, GSM5274, GSM5275, GSM5276, GSM5277 
Series (1)
GSE365 DNA repair in Mycobacterium tuberculosis

Data table header descriptions
ID NIH mAdb system Well_ID
Oligo_ID Operon Oligo Identifier
ORF First ORF column represents Sanger Institute H37RV_ORF_name; second ORF column represents The Institute for Genomic Research (TIGR) CDC1551_ORF_name
ORF First ORF column represents Sanger Institute H37RV_ORF_name; second ORF column represents The Institute for Genomic Research (TIGR) CDC1551_ORF_name
GENE_SYMBOL Gene Symbol/Name
DESCRIPTION Gene Description
SEQUENCE Sequence of oligonucleotide probe, provided by Operon

Data table
ID Oligo_ID ORF ORF GENE_SYMBOL DESCRIPTION SEQUENCE
1008070 T002214_01 Rv2214c MT2270 ephD probable epoxide hydrolase TCCGCGGTCAAGAAGAAGAAGCCGATCAGACCGGTCGCGCCGGAAGCCTATGCGCTATACGGCATCTCCC
1007341 T001485_01 Rv1485 MT1532 hemZ ferrochelatase ACGCGGTGGCCGAGCACTACCTGCATTTCGGTGGGGTATCACCGATCAATGGCATTAATCGCACACTGAT
1006612 T000756_01 Rv0756c Rv0756c hypothetical protein CCAAACAAGCGTTGGGTGATGCCGGCGGCGCAAGCGGGTCGACCTCAATGGCCAACATGCTGGGAATCGA
1008071 T002215_01 Rv2215 MT2272 sucB dihydrolipoamide succinyltransferase GACCCGCGAATCTTTGCAGGCAACGGCACAGCTGACACAAACCCATGAGGTCGACATGACCAAGATCGTG
1007342 T001486_01 Rv1486c Rv1486c conserved hypothetical protein ATCATGTGGTGTCCCAGTGTTTCGCTGTCGATCTGGGCTAACGCTTGGCTCGCCGGCAAGGCTGCTCCCG
1006613 T000757_01 Rv0757 MT0782 phoP two-component response regulator TGCTGAGCAAGCCTAAGATTCTCGACCACGTTTGGCGCTACGACTTCGGTGGTGATGTCAACGTCGTCGA
1008072 T002216_01 Rv2216 MT2273 Rv2216 conserved hypothetical protein GCGGTGGGCTACTACGGAAACACCAAGGACCGGGTGGTCGACGAAAACGACTCGGCGGGAACAGGTTTTC
1007343 T001487_01 Rv1487 Rv1487 conserved hypothetical protein CGAACCTGGTGACTCGGTGACCGTGGTGCAAATCGACGGCGCCACGGCGGTGGTCTTCAAGGACGTGTAG
1006614 T000758_01 Rv0758 MT0783 phoR sensor histidine kinase GGTTCTACGTTCGGGTGATCAGCCCCGACGGCCAGAGCTATACGGCACTCAACGACAACACTGCCATACC
1008073 T002217_01 Rv2217 MT2274 lipB lipoate biosynthesis protein B CCGATCATCGGGCTGGCCGAACCCCTCGACGTGGTCAATTACGTTCGGCGCCTTGAAGAATCGCTGATCC
1007344 T001488_01 Rv1488 MT1533.2 Rv1488 conserved hypothetical protein GTCTACTTCCAGGTGACCGTTCCGCAGGCGGCGGTGTACGAGATCAGCAATTACATCGTCGGGGTCGAAC
1006615 T000759_01 Rv0759c MT0784 Rv0759c conserved hypothetical protein GTGTTTCCCACCCGCAGCCTCAGTGATTTCGGCTTTGCCAACGTCGACCGCAACCCCTCACCGGGATCGC
1008074 T002218_01 Rv2218 MT2275 lipA lipoate biosynthesis protein A AGGAGGCCGGCTGCCCCAACATCTTCGAATGCTGGGAGGACCGAGAAGCCACCTTCCTGATCGGCGGTGA
1007345 T001489_01 Rv1489c Rv1489c hypothetical protein GGAATCGCATTAACAACGCGATCTCGCACCCGGACATGTGCCCCCACGGCGAGGATGAAGTACAACGTCA
1006616 T000760_01 Rv0760c MT0785 Rv0760c conserved hypothetical protein GGCGGCTTTACCAGTGAGGTCCGCGGCGTGTTCACCTACCGGGTGAATAAGGCCGGTTTGATCACCAACA
1008075 T002219_01 Rv2219 MT2276 Rv2219 hypothetical protein GTGTCATCTTCGTCGGCGAGGGATCAGCGGCCCGCGTCAAACCACTGCTGGCTCAGGAGAAAAAGCGCAC
1007346 T001490_01 Rv1490 MT1536 Rv1490 unknown putative membrane protein AGCGCAGGATAATGCTTGCCCTCCTCGTTCCGGTGCTACCCTTTGCCTTTTCTTACGCCATCTATAATCC
1006617 T000761_01 Rv0761c MT0786 adhB zinc-containing alcohol dehydrogenase GACCCTGCTGCAAAAGAACATCCAGGGCACCATCTTCGGCGGCGGCAACCCGCACTACGACATTCCGAAG
1008076 T002220_01 Rv2220 MT2278 glnA1 glutamine synthase class I GACGACATGCAGTTGTACAAGTACATCATCAAGAACACCGCCTGGCAGAACGGCAAAACGGTCACGTTCA
1007347 T001491_01 Rv1491c MT1538 Rv1491c probable membrane protein TCGGTATGCACGGGCGCGCTAGGTCTGACTGGGCTCGTCTATGAGATCCGCAATTATCGACGGCAGCACC

Total number of rows: 4416

Table truncated, full table size 580 Kbytes.






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