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Series GSE11308 Query DataSets for GSE11308
Status Public on Oct 04, 2008
Title Quantification of vascular lineage-specific differentiation
Organism Homo sapiens
Experiment type Expression profiling by array
Summary Angiogenesis and lymphangiogenesis have important roles in cancer progression and chronic inflammatory diseases, but efficient therapies against these diseases have been hampered by the lack of identified vascular lineage-specific markers and growth factors. Using transcriptional profiling of matched pairs of human dermal blood vascular and lymphatic endothelial cells, we first identified 236 lymphatic and 342 blood vascular signature genes. In silico analyses of the biologic pathways associated with these genes revealed lineage-specific functions for each cell type. Using a selection of 85 identified vascular lineage-specific genes, we developed a TaqMan RT-PCR-based, microfluidic card-formatted low-density microvascular differentiation array (LD-MDA) that was used to reliably identify and quantify the degree of lineage-specific differentiation in different types of endothelial cells, and to detect admixture of lymphatic endothelial cells in commercial preparations of microvascular endothelial cells. Application of Prediction Relevance Ranking and analysis of variance of LD-MDA expression profiles of 43 lesional skin samples obtained from patients with the chronic inflammatory disease psoriasis led to identification of cytokines which are significantly associated with angiogenesis or lymphangiogenesis in vivo. In particular, interleukin-7 and fibroblast growth factor-12 were identified as novel (lymph)angiogenic factors. This technology provides a novel tool to quantify lineage-specific vascular differentiation and to characterize (lymph)angiogenesis in clinical samples obtained from angiogenic diseases.

This SuperSeries is composed of the SubSeries listed below.
 
Overall design Refer to individual Series
 
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Submission date Apr 30, 2008
Last update date Dec 28, 2012
Contact name Jay Woo Shin
E-mail(s) jay.shin@gsc.riken.jp
Organization name ETH Zurich
Department Institute of Pharmaceutical Sciences
Lab Michael Detmar
Street address Wolfgang-Paulistrasse 10; HCI H394
City Zurich
State/province Zurich
ZIP/Postal code 8093
Country Switzerland
 
Platforms (2)
GPL6802 Low Density Microvascular Differentiation Array (LD-MDA) version 1.0
GPL6803 Low Density Microvascular Differentiation Array (LD-MDA) version 2.0
Samples (72)
GSM285656 Human dermal lymphatic endothelial cell 1
GSM285657 Human dermal lymphatic endothelial cell 2
GSM285658 Human dermal lymphatic endothelial cell 3
This SuperSeries is composed of the following SubSeries:
GSE11306 Quantification of vascular lineage-specific differentiation (cell type comparison)
GSE11307 Quantification of vascular lineage-specific differentiation, psoriasis (chronic inflammation) study
Relations
BioProject PRJNA106615

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Supplementary data files not provided
Raw data provided as supplementary file
Processed data included within Sample table

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