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Series GSE68643 Query DataSets for GSE68643
Status Public on Mar 03, 2016
Title PBX3 cooperates with MEISI in causing rapid acute myeloid leukemia and recapitulates the core transcriptome of MLL-rearranged leukemia
Organism Mus musculus
Experiment type Expression profiling by array
Summary To investigate whether co-expression of PBX3/MEIS1 can mimic that of MLL-AF9, HOXA9/MEIS1 or HOXA9/PBX3 in inducing leukemogenesis, we conducted in vivo mouse bone marrow transplantation (BMT) assays. Briefly, normal mouse bone marrow (BM) progenitor (i.e., lineage negative; Lin-) cells collected from B6.SJL (CD45.1) donor mice (CD45.1) were retrovirally co-transduced with MSCVneo-MLL-AF9+MSCV-PIG (MLL-AF9), MSCVneo-HOXA9+MSCV-PIG (HOXA9), MSCVneo-HOXA9+MSCV-PIG-MEIS1 (HOXA9+MEIS1), MSCVneo-HOXA9+MSCV-PIG-PBX3 (HOXA9+PBX3), MSCV-PIG-PBX3+MSCVneo-MEIS1 (PBX3+MEIS1), MSCVneo+MSCV-PIG-PBX3 (PBX3) , MSCVneo+MSCV-PIG-MEIS1 (MEIS1), or MSCVneo+MSCV-PIG (normal control; NC). Retrovirally transduced cells then were cultured with cytokines as well as puromycin and G418. Seven days later, the donor cells were transplanted into lethally irradiated (960 rads) 8- to 10-week-old C57BL/6 (CD45.2) recipient mice. The transplanted mice were watched for leukemogenesis. Then, gene expression profiling was conducted with bone marrow samples collected from leukemia groups and control group.
 
Overall design A total of 20 mouse BM samples including 15 mouse AML samples with 3 each of the MLL-AF9, HOXA9+MEIS1, HOXA9+PBX3, HOXA9, and PBX3+MEIS1 groups, along with 5 samples from the normal control (NC) group, were analyzed by use of Affymetrix GeneChip Mouse Gene 2.0 ST Array (Affymetirx, Santa Clara, CA). For each sample, the CD45.1+ cells (i.e., transplanted donor cells) were sorted with flow cytometry from whole BM cells collected from BMT recipient mice at the end stage. Then total RNA was isolated by use of miRNeasy extraction kit (Qiagen, Valencia, CA).
 
Contributor(s) Chen J, Li Z, Chen P, Zuo Z, Elkahloun A, Liu PP
Citation(s) 26747896
Submission date May 07, 2015
Last update date Feb 21, 2018
Contact name Jianjun Chen
E-mail(s) jianchen@coh.org
Phone 6262185591
Organization name Beckman Research Institute of City of Hope
Department Systems Biology
Lab Chen Lab
Street address 1218 S. 5th Ave., Room 2062
City Monrovia
State/province CA
ZIP/Postal code 91016
Country USA
 
Platforms (1)
GPL16570 [MoGene-2_0-st] Affymetrix Mouse Gene 2.0 ST Array [transcript (gene) version]
Samples (20)
GSM1677966 PL01 NC_1
GSM1677967 PL08 NC_2
GSM1677968 PL16 NC_3
Relations
BioProject PRJNA283287

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Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE68643_RAW.tar 203.5 Mb (http)(custom) TAR (of CEL)
Processed data included within Sample table

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