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| Status |
Public on Feb 14, 2007 |
| Title |
Oxygen-dependent transcriptome profiling in R.sphaeroides 2.4.1 in the dark |
| Platform organism |
Cereibacter sphaeroides 2.4.1 |
| Sample organism |
Cereibacter sphaeroides |
| Experiment type |
Expression profiling by array
|
| Summary |
Wild type (2.4.1) strain, dark conditions, controlled oxtgen concentration in the growth medium (achieved by constant sparging with a gas mixture containing 1% CO2, variable - indicated for every sample - concentration O2 and balance N2).
Keywords: dose response
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| Overall design |
Cells were harvested at OD 0.16-0.18 after rapid addition of rifampicin, spun down and frozen for subsequent RNA isolation. RNA was isolated using bead beater and RNeasy kit. Traces of DNA were removed by DNAse I treatment until level non-detectable by 33-36 cycles of real-time PCR. Further sample preparaton -according Affymetrix protocols. Data processing: MAS 5.0 values are deposited here (for compatability with other submitted samples), values used in the publications are obtained with RMA and may be recreated using the supplied CEL files.
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| Contributor(s) |
Moskvin O, Gomelsky M |
| Citation(s) |
17660296 |
| |
| Submission date |
Feb 10, 2007 |
| Last update date |
Apr 26, 2014 |
| Contact name |
Mark Gomelsky |
| E-mail(s) |
gomelsky@uwyo.edu
|
| Phone |
307-766-3522
|
| Organization name |
University of Wyoming
|
| Department |
Molecular Biology
|
| Lab |
Gomelsky
|
| Street address |
|
| City |
Laramie |
| State/province |
WY |
| ZIP/Postal code |
82071 |
| Country |
USA |
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| Platforms (1) |
| GPL162 |
[uwyRhodo1a] Rhodobacter sphaeroides 2.4.1 GeneChip |
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| Samples (14)
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| Relations |
| BioProject |
PRJNA99251 |
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