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Status
Public on Oct 30, 2003
Title
pilot 1D cold
Sample type
RNA
Source name
Ecotype Columbia, Light grown 7-day old seedlings, treated at 4 degrees C for 24hrs
Organism
Arabidopsis thaliana Extracted molecule
total RNA
Description
Total RNA was isolated from seedlings with the TRIzol reagent procedure of Invitrogen, then poly(A)+ RNA was purified with Qiagen oligotex. One microgram poly(A)+ RNA was converted into ds-cDNA using T7-oligo(dT) primers. Biotin-labeled cRNA was generated by in vitro transcription reactions using ENZO labeling kit, fragmented and then 20 micrograms of fragmented cRNA were hybridized to the arrays according to Affymetrix instructions.
Submission date
Aug 21, 2003
Last update date
May 28, 2005
Contact name
Atsunari Tsuchisaka
E-mail(s)
guixiay@yahoo.com, atsunaritsuchisaka@gmail.com Phone
510-559-5924
Organization name
University of California, Berkeley / USDA
Department
Plant Gene Expression Center
Street address
800 Buchanan St.
City
Albany
State/province
CA
ZIP/Postal code
94710
Country
USA
Platform ID
GPL431 Series (5)
GSE604 Identification of new full length Arabidopsis genes - pilot arrays
GSE636 Expression of Annotated Genes
GSE637 Antisense Expression of Annotated Genes
GSE638 Expression of Intergenic Clusters
GSE639 Expression in Intergenic Regions
Data table header descriptions ID_REF
SIGNAL_MEAN Mean signal intensity of the cell
SIGNAL_SD Standard deviation of signal intensity of the cell
SIGNAL_AREA Number of pixels used to calculate mean signal intensity
VALUE Same as SIGNAL_MEAN
Data table ID_REF SIGNAL_MEAN SIGNAL_SD SIGNAL_AREA VALUE 1
272.0
118.0
25
272.0
2
12293.8
2929.5
20
12293.8
3
301.0
55.4
25
301.0
4
12489.0
3191.7
25
12489.0
5
183.5
28.8
20
183.5
6
233.0
33.6
25
233.0
7
12421.0
3334.9
25
12421.0
8
276.5
41.0
20
276.5
9
12318.0
3594.7
25
12318.0
10
279.0
51.0
25
279.0
11
12471.3
934.5
16
12471.3
12
288.0
78.2
20
288.0
13
12500.5
1555.3
20
12500.5
14
280.5
37.7
16
280.5
15
12075.8
2404.9
20
12075.8
16
293.8
39.9
20
293.8
17
12430.3
1517.4
20
12430.3
18
281.0
41.5
16
281.0
19
12273.8
1528.4
20
12273.8
20
271.0
45.6
20
271.0
409600 10659 Kbytes .Supplementary data files not provided
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