GEO DataSets

(Submitter supplied) The glycopeptide vancomycin is a drug of choice for the treatment of severe non-gastrointestinal infections with members of Bacillus cereus sensu lato. Recently, sporadic detection of vancomycin resistant phenotypes emerged, mostly for agar diffusion testing. The food packaging isolate BC70 displayed a pseudo-resistant phenotype for vancomycin in both Etest and disk diffusion. In this work, we used RNA-Seq on the nanopore platform to study differentially expressed genes in BC70 cells, which were able to actively move into the inhibition zone during vancomycin susceptibility testing using Etest and therefore appeared to be resistant. more...

Organism:

Bacillus cereus

Type:

Expression profiling by high throughput sequencing

Platform:

GPL34081

4 Samples

Download data: CSV

(Submitter supplied) The iron-binding glycoprotein lactoferrin exhibits anti-bacterial activity. To investigate the overall response of Bacillus cereus to lactoferrin, total transcriptome analyses were performed. Growth conditions were mMOD minimal medium, minimal medium plus 10 mg/ml lactoferrin, as well as minimal medium without iron source for comparison. Differential regulation of genes involved in iron acquisition as well as genes encoding enterotoxins and further virulence factors was of special interest.

Organism:

Bacillus cereus

Type:

Expression profiling by high throughput sequencing

Platform:

GPL26265

6 Samples

Download data: GCT, GFF, TSV, TXT, XLSX

(Submitter supplied) Background: The Bacillus cereus Sigma B (SigB) dependent general stress response is activated via the two-component RsbKY system, which involves a phosphate transfer from RsbK to RsbY. It has been hypothesized that the Hpr-like phosphocarrier protein (Bc1009) encoded by bc1009 in the SigB gene cluster may play a role in this transfer, thereby acting as a regulator of SigB activation. Alternatively, Bc1009 may be involved in the activation of a subset of SigB regulon members. more...

Organism:

Bacillus cereus ATCC 14579

Type:

Expression profiling by array

Platform:

GPL9493

16 Samples

Download data: TXT

(Submitter supplied) Transcriptome analyses after the transfer of pCER270-spec megaplasmid in B. cereus F4810-72 (previously cured for its plasmid), B. thuringiensis HD73, and B. weihenstephanensis KBAB6 strains. We determined differentially expressed genes in pCER270+ strains in comparison with pCER270- strains.

Organism:

Bacillus cereus; Bacillus thuringiensis; Bacillus mycoides

Type:

Expression profiling by high throughput sequencing

Platforms:

GPL29834 GPL32378 GPL32936

36 Samples

Download data: TXT

(Submitter supplied) Bacillus cereus is the second leading cause of collective food poisoning in France. B. cereus is also associated with severe clinical infections leading to patient death in 10% of the cases. The emergence of B. cereus as a foodborne and opportunistic pathogen has intensified the need to distinguish strains of public health concern. In this work, by performing a screen on a large collection of B. cereus strains of varying pathogenic potential, we identified genetic determinants capable of discriminating B. more...

Organism:

Bacillus cereus

Type:

Expression profiling by high throughput sequencing

Platform:

GPL29834

45 Samples

Download data: CSV

(Submitter supplied) Bacterial response to nitric oxide (NO) is of major importance for bacterial survival, as NO stress is a main actor of the eukaryote immune response. Several pathogenic bacteria have developed gene regulation systems involved in detoxification and in repairing the damages caused by NO. However, bacterial mechanisms of NO resistance are poorly described for Gram positive bacteria and especially Bacillus cereus. more...

Organism:

Bacillus cereus

Type:

Expression profiling by high throughput sequencing

Platform:

GPL29834

21 Samples

Download data: CSV

(Submitter supplied) In the intestine, B. cereus comes in contact with the epithelial mucus layer. To investigate the overall response to that contact, total transcriptome analyses were performed using porcine gastric mucin (PGM), which is a commonly applied model for mucus interactions. Differential regulation of genes encoding enterotoxins and further virulence factors was of special interest.

Organism:

Bacillus cereus

Type:

Expression profiling by high throughput sequencing

Platform:

GPL26265

6 Samples

Download data: TSV

(Submitter supplied) We investigated an improved method that combines competitive PCR and microarray techniques. This approach allowed us to quantify specific bacterial groups mounted on DNA chips with accuracy close to that of real-time PCR, despite a measurement at the end point of PCR, and also to estimate the bacterial DNA content in sample DNA.

Organism:

Bacteria; Acinetobacter baumannii; Escherichia coli; Staphylococcus aureus; Staphylococcus epidermidis; Bacillus cereus; Schaalia odontolytica; Fusobacterium nucleatum subsp. nucleatum; Treponema denticola; Cereibacter sphaeroides; Streptococcus gordonii; Streptococcus agalactiae; Enterococcus faecalis; Bifidobacterium adolescentis; Homo sapiens; Prevotella intermedia; Prevotella nigrescens; Campylobacter rectus; Helicobacter pylori; Pseudomonas aeruginosa; Aggregatibacter actinomycetemcomitans; Phocaeicola vulgatus; Capnocytophaga gingivalis; Deinococcus radiodurans; Streptococcus mutans; Streptococcus intermedius; Cutibacterium acnes; Neisseria meningitidis; Porphyromonas gingivalis; Fusobacterium nucleatum; Clostridium beijerinckii; Lactobacillus gasseri; Tannerella forsythia

Type:

Other

Platform:

GPL25612

178 Samples

Download data: CSV

(Submitter supplied) Most differential expression point to attack and defense strategies, comprising structural and metabolic changes.

Organism:

Bacillus cereus ATCC 14579

Type:

Expression profiling by high throughput sequencing

Platform:

GPL25160

14 Samples

Download data: RPKM, TXT

(Submitter supplied) In this study we compared the transcriptomic profiles of cells grown in BHI, BHI supplemented with FeCl3, BHI supplemented with iron scavenger Bipyridine and both iron and the scavenger.

Organism:

Bacillus cereus ATCC 10987

Type:

Expression profiling by array

Platform:

GPL7681

24 Samples

Download data: TXT

(Submitter supplied) Heat-treated spores show delayed and slower germination and outgrowth compared to untreated spores presumably due to spore damage repair. This study was performed to identify genes possibly involved in spore damage repair in B. cereus. In this study we compared the transcriptomic profiles of untreated and heat-treated spores during germination and outgrowth in BHI at 30C.

Organism:

Bacillus cereus ATCC 14579

Type:

Expression profiling by array

Platform:

GPL19768

24 Samples

Download data: TXT, XLSX

(Submitter supplied) This SuperSeries is composed of the SubSeries listed below.

Organism:

Bacillus cereus ATCC 14579; Bacillus cereus ATCC 10987

Type:

Expression profiling by array

Platforms:

GPL7681 GPL7680

24 Samples

Download data: TXT

(Submitter supplied) Planktonic and biofilm cells of Bacillus cereus ATCC 14579 and ATCC 10987 were studied using microscopy and transcriptome analysis. By microscopy, clear differences could be observed between biofilm and planktonic cells as well as between the two strains. By using hierarchical clustering of the transcriptome data, little difference was observed between the biofilm cells of B. cereus ATCC 14579 and ATCC 10987. more...

Organism:

Bacillus cereus ATCC 10987

Type:

Expression profiling by array

Platform:

GPL7681

12 Samples

Download data: TXT

(Submitter supplied) Planktonic and biofilm cells of Bacillus cereus ATCC 14579 and ATCC 10987 were studied using microscopy and transcriptome analysis. By microscopy, clear differences could be observed between biofilm and planktonic cells as well as between the two strains. By using hierarchical clustering of the transcriptome data, little difference was observed between the biofilm cells of B. cereus ATCC 14579 and ATCC 10987. more...

Organism:

Bacillus cereus ATCC 14579

Type:

Expression profiling by array

Platform:

GPL7680

12 Samples

Download data: TXT

(Submitter supplied) RpoN was shown to have a pleiotropic role in different microorganisms. This study was performed to elucidate role in B. cereus. In this study we compared the transcriptomic profiles of the WT, rpoN mutant and the complemented strain in aerated and static growth conditions.

Organism:

Bacillus cereus ATCC 14579

Type:

Expression profiling by array

Platform:

GPL19768

24 Samples

Download data: TXT

(Submitter supplied) B. cereus RNA isolation were taken at OD 0.5 just before addition of 0.02 mg/mL EEC, and at 30 min of exposure. Water was added instead of EEC for control. Total RNA was isolated and we performed DNA microarray for genome-wide transcriptional analysis of B. cereus ATCC 14579 in the presence or absence of EEC. The microarray used in this study was custom-made B. cereus ATCC 14579 developed by Agilent Technologies (https://earray.chem.agilent.com/earray/). more...

Organism:

Bacillus cereus

Type:

Expression profiling by array

Platform:

GPL20186

8 Samples

Download data: TXT

(Submitter supplied) Pathogenic species belonging to Bacillus cereus sensu lato group possess a high evolutionary advantage in the environment and in food matrices thanks to their capacity to survive as silent spores to harsh environmental insults and grow at relatively low temperatures. Ready to re-heat products are at severe risk for contamination by members of Bacillus cereus s.l. group if not stored at proper conditions. more...

Organism:

[Bacillus thuringiensis] serovar konkukian str. 97-27; Bacillus cereus

Type:

Expression profiling by array

Platform:

GPL19697

12 Samples

Download data: TXT

(Submitter supplied) The influence of the presence of glucose in the Y1 growth medium of Bacillus cereus strain ATCC 14579 was studied by transcriptional analysis. Furthermore, the role of CcpA in glucose induction or repression of gene expression was assessed by use of a ccpA deletion strain. In total, 300 genes were glucose repressed and 173 genes glucose activated. For 212 genes the glucose repression was clearly CcpA dependent, whereas for 116 genes CcpA dependent glucose induction was observed. more...

Organism:

Bacillus cereus ATCC 14579

Type:

Expression profiling by array

Platform:

GPL7680

8 Samples

Download data

(Submitter supplied) Members of the Bacillus cereus group can adapt to a wide range of environmental challenges. In bacteria, these challenges are often translated into a transcriptional response via the cognate response regulators (RRs) of specialized two-component systems (TCSs). We have previously developed a phylogenetic footprinting approach that was successfully implemented to predict specific binding sites (operators) and target genes for the RRs of B. more...

Organism:

Bacillus cereus ATCC 14579

Type:

Expression profiling by array

Platform:

GPL8114

4 Samples

Download data

(Submitter supplied) Aims: To investigate the inactivation kinetics of Bacillus cereus vegetative cells upon exposure to low-temperature nitrogen gas plasma and to reveal the mode of inactivation by transcriptome profiling. Methods and results: Exponentially growing B. cereus cells were filtered and put on agar plates. The plates, carrying the filters with the vegetative cells, were placed into nitrogen gas plasma at atmospheric pressure and cold temperature (~37°C). more...

Organism:

Bacillus cereus ATCC 14579; Bacillus cereus

Type:

Expression profiling by array

Platform:

GPL9493

16 Samples

Download data: TXT