GEO DataSets

(Submitter supplied) Premetamorphic Xenopus laevis tadpole tail respond to thyroid hormone by resorption. The goal of this experiment is to identify the genes involved in the TH-induced resorption tadpole tail and compare it to TH-induced proliferation and differentiation program in tadpole limb and brain. Xenopus tadpoles (NF54) were treated with 100 nM T3 in 0.1 x MMR for another 24h and 48h or without T3 for 48h (control group). more...

Organism:

unidentified; Xenopus laevis

Type:

Expression profiling by array

Dataset:

GDS1719

Platform:

GPL2901

12 Samples

Download data: TIFF

(Submitter supplied) Premetamorphic Xenopus laevis tadpoles limb bud cells respond to thyroid hormone by proliferation and subsequent differentiation. The goal of this experiment is to identify the genes involved in the TH-induced proliferation pathway in developing tadpole limb bud and compare it to TH-induced proliferation and differentiation program in tadpole brain. Xenopus tadpoles (NF54) were treated with 1 mM methimazole in 0.1 X MMR solution for 1 week to block the endogenous TH production and reduce the TH present in the system of the tadpole. more...

Organism:

unidentified; Xenopus laevis

Type:

Expression profiling by array

Dataset:

GDS1718

Platform:

GPL2901

12 Samples

Download data: TIFF

(Submitter supplied) Premetamorphic Xenopus laevis tadpoles brain ventricle cells respond to thyroid hormone by proliferation and subsequent differentiation. The goal of this experiment is to identify the genes involved in the TH-induced proliferation pathway in tadpole brain and compare it to TH-induced proliferation and differentiation program in tadpole limb. Xenopus tadpoles (NF54) were treated with 1 mM methimazole in 0.1 X MMR solution for 1 week to block the endogenous TH production and reduce the TH present in the system of the tadpole. more...

Organism:

unidentified; Xenopus laevis

Type:

Expression profiling by array

Dataset:

GDS1717

Platform:

GPL2901

9 Samples

Download data: TIFF

Analysis of tails of NF stage 54 (NF54) premetamorphic tadpoles treated with thyroid hormone (TH) triiodothyronine (T3) up to 48 hours. NF61 tadpoles were in 0.1xMMR till they reached NF62. Premetamorphic tails are resorbed in response to TH. Results identify genes involved in TH-induced resorption.

Organism:

Xenopus laevis; unidentified

Type:

Expression profiling by array, log ratio, 2 agent, 2 development stage, 3 time sets

Platform:

GPL2901

Series:

GSE3405

12 Samples

Download data: TIFF

Analysis of limb buds of NF stage 54 (NF54) premetamorphic tadpoles treated with thyroid hormone (TH) triiodothyronine (T3) for 14, 24 or 48 hours. Premetamorphic limb buds proliferate and differentiate in response to TH. Results identify genes involved in the TH-induced proliferation pathway.

Organism:

Xenopus laevis; unidentified

Type:

Expression profiling by array, log ratio, 2 agent, 3 time sets

Platform:

GPL2901

Series:

GSE3404

12 Samples

Download data: TIFF

Analysis of brains of NF stage 54 (NF54) premetamorphic tadpoles treated with thyroid hormone (TH) triiodothyronine (T3) for 24 or 48 hours. Premetamorphic brain ventricle cells proliferate and differentiate in response to TH. Results identify genes involved in the TH-induced proliferation pathway.

Organism:

Xenopus laevis; unidentified

Type:

Expression profiling by array, log ratio, 2 agent, 2 time sets

Platform:

GPL2901

Series:

GSE3402

9 Samples

Download data: TIFF

(Submitter supplied) Thyroid hormones (TH), thyroxine (T4) and 3, 5, 3’-triiodothyronine (T3), play crucial roles in regulation of growth, development and metabolism in vertebrates and are targets for endocrine disruptive agents. Perturbations in TH action can contribute to the development of disease states and the U.S. Environmental Protection Agency is developing a high throughput screen using TH-dependent metamorphosis of the Xenopus laevis tadpole as an assay platform. more...

Organism:

Xenopus laevis; Aquarana catesbeiana

Type:

Expression profiling by array

Platform:

GPL4801

64 Samples

Download data

(Submitter supplied) Conclusions: Our study represents the first detailed analysis of hindlimb transcriptomes in wild-type and TRα (-/-) tadpoles, with biologic replicates, generated by RNA-seq technology. The optimized data analysis workflows reported here should provide a framework for comparative investigations of expression profiles.

Organism:

Xenopus tropicalis

Type:

Expression profiling by high throughput sequencing

Platform:

GPL21875

12 Samples

Download data: TXT

(Submitter supplied) Our study represents the first detailed analysis of thyroid hormone binding to the genome in the hindlimb of wild-type and TRα (-/-) tadpoles, with biological replicates, generated by ChIP-seq technology. The optimized data analysis workflows reported here should provide a framework for comparative investigations of TR binding profiles.

Organism:

Xenopus tropicalis

Type:

Genome binding/occupancy profiling by high throughput sequencing

Platform:

GPL21875

12 Samples

Download data: TXT

(Submitter supplied) This SuperSeries is composed of the SubSeries listed below.

Organism:

Xenopus laevis

Type:

Expression profiling by array

Platforms:

GPL3936 GPL8463 GPL2901

34 Samples

Download data: TXT

(Submitter supplied) Thyroid hormone (TH) controls the remodeling of the pancreas and the liver. TH-induces dedifferentiation of the exocrine pancreas to a progenitor state (Proc. Nat. Acad Sci. 105, 8962-8967 (2008)) and it remodels the endocrine pancreas (Dev. Biol. 328, 384-391 (2009)). The redifferentiated frog pancreas resembles closely the pancreas of other typical vertebrates. Two pancreas arrays were carried out. more...

Organism:

Xenopus laevis

Type:

Expression profiling by array

Platform:

GPL8463

16 Samples

Download data: TXT

(Submitter supplied) Thyroid hormone (TH) controls the remodeling of the pancreas and the liver. TH-induces dedifferentiation of the exocrine pancreas to a progenitor state (Proc. Nat. Acad Sci. 105, 8962-8967 (2008)) and it remodels the endocrine pancreas (Dev. Biol. 328, 384-391 (2009)). The redifferentiated frog pancreas resembles closely the pancreas of other typical vertebrates. Two pancreas arrays were carried out. more...

Organism:

Xenopus laevis

Type:

Expression profiling by array

Platform:

GPL3936

9 Samples

Download data: TXT

(Submitter supplied) Thyroid hormone (TH) controls the remodeling of the pancreas and the liver. TH-induces dedifferentiation of the exocrine pancreas to a progenitor state (Proc. Nat. Acad Sci. 105, 8962-8967 (2008)) and it remodels the endocrine pancreas (Dev. Biol. 328, 384-391 (2009)). The redifferentiated frog pancreas resembles closely the pancreas of other typical vertebrates. Two pancreas arrays were carried out. more...

Organism:

Xenopus laevis

Type:

Expression profiling by array

Platform:

GPL2901

9 Samples

Download data: TXT

(Submitter supplied) Premetamorphic Xenopus laevis tadpoles limbs develop in response to thyroid hormone by proliferation and subsequent differentiation. We have previously reported that inhibiting the thyroid hormone action using a dominant negative receptor in limbs of developing tadpoles inhibits its growth and the tadpole limb has almost no muscle. The goal of this experiment is to study the role of thyroid hormone in the maintenance of limb muscle using transgenic tadpoles carrying a doxycyline inducible dominant negative thyroid receptor transgene expressed exclusively in the muscle using a muscle specific promoter and identify the genes involved in the degenerative process . more...

Organism:

Xenopus laevis

Type:

Expression profiling by array

Dataset:

GDS2120

Platform:

GPL3936

12 Samples

Download data: TXT

Analysis of hind limbs of premetamorphic (NF55) and postmetamorphic (NF66) transgenics 2 or 6 weeks following transcriptional induction of a dominant negative thyroid hormone (TH) receptor transgene. Results provide insight into the role of TH in the maintenance of limb muscle during development.

Organism:

Xenopus laevis

Type:

Expression profiling by array, log10 ratio, 2 development stage, 2 genotype/variation, 2 time sets

Platform:

GPL3936

Series:

GSE5249

12 Samples

Download data: TXT

(Submitter supplied) Association of thyroid hormone receptors (TRs) in chromatin across the genome of neural cells in the preoptic area/hypothalamus/thalamus of metamorphic climax stage Xenopus tropicalis tadpoles, analyzed by chromatin-immunoprecipitation sequencing (ChIP-seq).

Organism:

Xenopus tropicalis

Type:

Genome binding/occupancy profiling by high throughput sequencing

Platform:

GPL21874

2 Samples

Download data: TXT

(Submitter supplied) Changes in gene expression in the preoptic area/hypothalamus/thalamus of Xenopus tropicalis tadpoles were analyzed using RNAseq at four stages of metamorphosis.

Organism:

Xenopus tropicalis

Type:

Expression profiling by high throughput sequencing

Platform:

GPL21874

12 Samples

Download data: FA, MATRIX, TXT

(Submitter supplied) Changes in DNA methylation in the preoptic area/hypothalamus/thalamus of Xenopus tropicalis tadpoles were analyzed using Methylated DNA Capture sequencing (MethlCap-seq) at four stages of metamorphosis.

Organism:

Xenopus tropicalis

Type:

Methylation profiling by high throughput sequencing

Platform:

GPL21874

15 Samples

Download data: BED

(Submitter supplied) This SuperSeries is composed of the SubSeries listed below.

Organism:

Xenopus laevis; Aquarana catesbeiana

Type:

Expression profiling by array

Platform:

GPL4800

124 Samples

Download data

(Submitter supplied) Thyroid hormones (TH), thyroxine (T4) and 3, 5, 3’-triiodothyronine (T3), play crucial roles in regulation of growth, development and metabolism in vertebrates and their action are targets for endocrine disruptive agents. Perturbations in TH action can contribute to the development of disease states and the U.S. Environmental Protection Agency is developing a high throughput screen using TH-dependent amphibian metamorphosis as an assay platform. more...

Organism:

Xenopus laevis; Aquarana catesbeiana

Type:

Expression profiling by array

Platform:

GPL4800

89 Samples

Download data