GEO DataSets

(Submitter supplied) We are investigating the transcriptional response of yeast to treatment with enediynes or gamma radiation, which generate different extents of double or single strand breaks in DNA. We used microarrays to detail the global programme of gene expression underlying the DNA damage response in yeast Keywords: dose

Organism:

Saccharomyces cerevisiae

Type:

Expression profiling by array

Dataset:

GDS2508

Platform:

GPL90

18 Samples

Download data

Analysis of cells exposed to the radiomimetic enediyne antibiotics calicheamicin, esperamicin A1, and neocarzinostatin. These enediynes bind specifically to DNA and generate single- and double-strand DNA breaks. Results provide insight into the response to DNA damage.

Organism:

Saccharomyces cerevisiae

Type:

Expression profiling by array, count, 6 agent sets

Platform:

GPL90

Series:

GSE5301

18 Samples

Download data

(Submitter supplied) Wt S. cerevisiae cells from a W303 background were exposed to multiple doses of MMS and Gamma Radiation and their global transcriptional response was measured Keywords: dose response

Organism:

Saccharomyces cerevisiae

Type:

Expression profiling by array

Platform:

GPL4416

24 Samples

Download data: TXT

(Submitter supplied) We are investigating the transcriptional response of yeast to modulation of the expression of base excision repair players, these generate different dna lesions of abasic sites of strand breaks We used microarrays to detail the global programme of gene expression underlying the DNA damage response in yeast Keywords: dose

Organism:

Saccharomyces cerevisiae

Type:

Expression profiling by array

Platform:

GPL90

18 Samples

Download data: CEL

(Submitter supplied) The ability to survive stress conditions is important for every living cell. Some stresses can affect not only current cell well-being, but may have far-reaching consequences. Uncurbed oxidative stress can cause DNA damage and the decrease in cell survival and/or increase in mutation rate. Some substances generating oxidative damage in the cell act mainly on DNA. Radiomimetic zeocin is a chemoenzyme that causes oxidative damage in DNA, inducing predominantly single or double strand breaks. more...

Organism:

Saccharomyces cerevisiae

Type:

Expression profiling by array

Platform:

GPL18909

4 Samples

Download data: TXT

(Submitter supplied) We have used the budding yeast Saccharomyces cerevisiae to identify genes that may confer sensitivity in vivo to the antimalarial and cytotoxic agent cryptolepine. To this end, five S. cerevisiae strains, which differ in the condition of genes related to cell membrane integrity and to DNA damage repair, were exposed to several concentrations of cryptolepine. Results showed a relatively mild toxicity of cryptolepine for wild type strains, which increased by either increasing cell permeability (∆erg6 or ISE2 strains) or disrupting DNA damage repair (∆rad52 strains). more...

Organism:

Saccharomyces cerevisiae

Type:

Expression profiling by array

Platform:

GPL4069

8 Samples

Download data: TXT

(Submitter supplied) Oxidative stress is a harmful condition in a cell, tissue, or organ, caused by an imbalnace between reactive oxygen species and other oxidants and the capacity of antioxidant defense systems to remove them. The budding yeast S. cerevisiae has been the major eukaryotic model for studies of response to oxidative stress. We used microarrays to study the genome-wide temporal response of the yeast S. cerevisiae to oxidative stress induced by cumene hydroperoxide. more...

Organism:

Saccharomyces cerevisiae

Type:

Expression profiling by array

Dataset:

GDS3035

Platform:

GPL90

48 Samples

Download data: CEL

Analysis of Saccharomyces cerevisiae cultures at various time points up to 120 minutes following treatment with the oxidant cumene hydroperoxide (CHP). Results provide insight into molecular mechanisms underlying the response of the yeast S. cerevisiae to oxidative stress.

Organism:

Saccharomyces cerevisiae

Type:

Expression profiling by array, transformed count, 2 agent, 8 time sets

Platform:

GPL90

Series:

GSE7645

48 Samples

Download data: CEL

(Submitter supplied) Numerous methods are available for the mapping of DNA lesions ranging from double-strand breaks (DSBs) to incorporated ribonucleotides. We now present a technology based on the Genomewide Ligation of 3’-OH Ends (GLOE-Seq) and an associated computational pipeline designed for single-stranded breaks (SSBs), but versatile enough to be applied to any lesion that is convertible into a free 3’-OH terminus. more...

Organism:

Saccharomyces cerevisiae

Type:

Other

Platform:

GPL19756

48 Samples

Download data: BW

(Submitter supplied) This SuperSeries is composed of the SubSeries listed below.

Organism:

Saccharomyces cerevisiae; Homo sapiens

Type:

Other

Platforms:

GPL19756 GPL18573

96 Samples

Download data: BW

(Submitter supplied) Numerous methods are available for the mapping of DNA lesions ranging from double-strand breaks (DSBs) to incorporated ribonucleotides. We now present a technology based on the Genomewide Ligation of 3’-OH Ends (GLOE-Seq) and an associated computational pipeline designed for single-stranded breaks (SSBs), but versatile enough to be applied to any lesion that is convertible into a free 3’-OH terminus. more...

Organism:

Homo sapiens

Type:

Other

Platform:

GPL18573

8 Samples

Download data: BW

(Submitter supplied) Numerous methods are available for the mapping of DNA lesions ranging from double-strand breaks (DSBs) to incorporated ribonucleotides. We now present a technology based on the Genomewide Ligation of 3’-OH Ends (GLOE-Seq) and an associated computational pipeline designed for single-stranded breaks (SSBs), but versatile enough to be applied to any lesion that is convertible into a free 3’-OH terminus. more...

Organism:

Saccharomyces cerevisiae

Type:

Other

Platform:

GPL19756

2 Samples

Download data: BW

(Submitter supplied) Numerous methods are available for the mapping of DNA lesions ranging from double-strand breaks (DSBs) to incorporated ribonucleotides. We now present a technology based on the Genomewide Ligation of 3’-OH Ends (GLOE-Seq) and an associated computational pipeline designed for single-stranded breaks (SSBs), but versatile enough to be applied to any lesion that is convertible into a free 3’-OH terminus. more...

Organism:

Saccharomyces cerevisiae

Type:

Other

Platform:

GPL19756

12 Samples

Download data

(Submitter supplied) Numerous methods are available for the mapping of DNA lesions ranging from double-strand breaks (DSBs) to incorporated ribonucleotides. We now present a technology based on the Genomewide Ligation of 3’-OH Ends (GLOE-Seq) and an associated computational pipeline designed for single-stranded breaks (SSBs), but versatile enough to be applied to any lesion that is convertible into a free 3’-OH terminus. more...

Organism:

Saccharomyces cerevisiae

Type:

Other

Platform:

GPL19756

4 Samples

Download data: BW

(Submitter supplied) Numerous methods are available for the mapping of DNA lesions ranging from double-strand breaks (DSBs) to incorporated ribonucleotides. We now present a technology based on the Genomewide Ligation of 3’-OH Ends (GLOE-Seq) and an associated computational pipeline designed for single-stranded breaks (SSBs), but versatile enough to be applied to any lesion that is convertible into a free 3’-OH terminus. more...

Organism:

Saccharomyces cerevisiae

Type:

Other

Platform:

GPL19756

22 Samples

Download data: BW

(Submitter supplied) The capacity of respiring cultures of Saccharomyces cerevisiae to instantaneously switch to fast alcoholic fermentation upon a transfer to anaerobic sugar-excess conditions is a key characteristic of Saccharomyces cerevisiae in many of its industrial applications. This transition was studied by exposing aerobic glucose-limited chemostat cultures grown at a low specific growth rate to two simultaneous perturbations: oxygen depletion and relief of glucose limitation. more...

Organism:

Saccharomyces cerevisiae

Type:

Expression profiling by array

Platform:

GPL90

13 Samples

Download data: CEL, CHP, EXP

(Submitter supplied) Physiological effects of carbon dioxide and impact on genome-wide transcript profiles were analysed in chemostat cultures of Saccharomyces cerevisiae. In anaerobic, glucose-limited chemostat cultures grown at atmospheric pressure, cultivation under CO2-saturated conditions had only a marginal (<10%) impact on the biomass yield. Conversely, a 25% decrease of the biomass yield was found in aerobic, glucose-limited chemostat cultures aerated with a mixture of 79% CO2 and 21% O2. more...

Organism:

Saccharomyces cerevisiae

Type:

Expression profiling by array

Dataset:

GDS2969

Platform:

GPL90

18 Samples

Download data: CEL, EXP

Analysis of chemostat cultures sparged with 79% or 100% carbon dioxide (CO2). During fermentation and ethanol production, CO2 is produced, saturating the fermentative broth. Results provide insight into the mechanisms underlying CO2 stress.

Organism:

Saccharomyces cerevisiae

Type:

Expression profiling by array, count, 4 dose, 2 growth protocol sets

Platform:

GPL90

Series:

GSE8900

18 Samples

Download data: CEL, EXP

(Submitter supplied) The objective is to identify genes that are differentially expressed following the introduction of DNA double-strand breaks (DSBs) by the Rag proteins in murine pre-B cells. Cells lacking Artemis are used since the Rag-induced DSBs will not be repaired, and thus, will provide a continuous stimulus to the cell.

Organism:

Mus musculus

Type:

Expression profiling by array

Platform:

GPL1261

6 Samples

Download data: CEL, CHP

(Submitter supplied) The objective of this set of samples is to identify genes that are differentially expressed following the introduction of DNA double strand breaks (DSBs) by ionizing radiation in wild-type murine pre-B cells. The data generated in this project will be compared to the data generated in GSE9024, in which genes that are differentially expressed following the introduction of DNA double strand breaks (DSBs) by the Rag proteins in murine pre-B cells were examined. more...

Organism:

Mus musculus

Type:

Expression profiling by array

Platform:

GPL1261

6 Samples

Download data: CEL, CHP