GEO DataSets

(Submitter supplied) Aims of study: (1) To identify systemic differences in osteoarthritic (OA) bone that contribute to OA pathogenesis. (2) Identify novel osteoporotic (OP) bone-related disease genes. Study involved comparison of trabecular bone extracted from the intertrochanteric (IT) region of the proximal femur (PF) from OA, OP and normal/control (CTL) individuals. Bone was obtained from OA and OP individuals at surgery for total hip replacement and from CTL individuals at autopsy. more...

Organism:

Homo sapiens

Type:

Expression profiling by array

Platform:

GPL5391

39 Samples

Download data

(Submitter supplied) Osteoarthritis (OA) is a complex degenerative joint disease, which is not only a cartilage but also a bone disease. A better understanding of the early molecular mechanism changes of subchondral bone in vivo may contribute to elucidating the pathogenesis of OA. We used microarray technology to investigate the time-course molecular changes of subchondral bone just beneath damaged cartilage in early stage of experimental osteoarthritis, and found 2,234 differentially expressed (DE) genes at 1 week, 1,944 at 2 weeks and 1,517 at 4 weeks postsurgery.Further analysis of dysregulated genes indicated that subchondral bone remodeling occurred sequentially and in a time-dependent manner at the gene expression level. more...

Organism:

Rattus norvegicus

Type:

Expression profiling by array

Platform:

GPL7294

30 Samples

Download data: TXT

(Submitter supplied) Osteoarthritic cartilage has largely been investigated, however supporting structures as the acetabular labrum are less investigated. In this studies we aimed to identify differences in gene expression between healthy and osteoarthritic labrum cells we used the microarray as a discovery tool to identify possible new targets in hip osteoarthritis pathology

Organism:

Homo sapiens

Type:

Expression profiling by array

Dataset:

GDS5427

Platform:

GPL6244

8 Samples

Download data: CEL

Analysis of osteoarthritic labrum cells cultured for 7 days in a 3-dimensional alginate bead system. The acetabular labrum is a fibrocartilaginous structure that seals the hip joint. Results provide insight into hip OA-associated molecular changes in acetabular labrum cells.

Organism:

Homo sapiens

Type:

Expression profiling by array, transformed count, 2 disease state, 8 individual sets

Platform:

GPL6244

Series:

GSE60762

8 Samples

Download data: CEL

(Submitter supplied) MC3T3 cells clone 1b were grown in alpha-MEM with 10% fetal calf serum (FCS, Gibco), 1% penicilin/streptomycin (Pen/Strep) and 1% L-Glutamine (L-Glu). For differentiation experiment, cells were seeded on 6 cm dishes (3x105cells/dish in 5 ml medium) and grownt to confluence for 3 days at 37C / 5% CO2. Cells were then stimulated with osteogenic stimulus (10 mM beta-glycerophosphate (GP, Sigma), 50 µM ascorbic acid (AA, Wako) and 1 mg/ml BMP-2 (Novartis)), or with 10mM GP alone for 1 or 3 days. more...

Organism:

Mus musculus

Type:

Expression profiling by array

Dataset:

GDS679

Platform:

GPL81

15 Samples

Download data

Response of calvarial MC3T3-1b cells to osteogenic stimulus (beta-glycerophosphate (GP), ascorbic acid (AA) and bone morphogenic protein 2 (BMP-2)) for 0, 1 and 3 days. Notch, Wnt and TGF-β signaling pathways are activated in osteogenesis.

Organism:

Mus musculus

Type:

Expression profiling by array, count, 3 growth protocol, 3 time sets

Platform:

GPL81

Series:

GSE1131

15 Samples

Download data

(Submitter supplied) To date, all of the prior osteoarthritic microarray studies in human tissue have focused on the overlying articular cartilage, meniscus, or synovium but not the underlying subchondral bone. In our previous study, our group developed a methodology for high quality RNA isolation from site-matched cartilage and bone from human knee joints, which allowed us to perform candidate gene expression analysis on the subchohndral bone (published on Osteoarthritis and Cartilage on Dec/5/2012 (doi: 10.1016/j.joca.2012.11.016). more...

Organism:

Homo sapiens

Type:

Expression profiling by array

Platform:

GPL13497

50 Samples

Download data: TXT

(Submitter supplied) Autologous chondrocyte transplantation (ACT) is a routine technique to regenerate focal cartilage lesions. However, patients with osteoarthritis (OA) are lacking an appropriate long-lasting treatment alternative, partly since it is not known if chondrocytes from OA patients have the same chondrogenic differentiation potential as chondrocytes from donors not affected by OA. Articular chondrocytes from patients with OA undergoing total knee replacement (Mankin Score >3, Ahlbäck Score >2) and from patients undergoing ACT, here referred to as normal donors (ND), were isolated applying protocols used for ACT. more...

Organism:

Homo sapiens

Type:

Expression profiling by array

Dataset:

GDS3758

Platform:

GPL570

12 Samples

Download data: CEL, EXP

Analysis of monolayer cultures and Hyaff-11 scaffold cultures of chondrocytes from 3 donors suffering from osteoarthritis (OA). Results provide insight into the differentiation process of OA chondrocytes and their possible use in matrix-associated autologous chondrocyte transplantation (ACT).

Organism:

Homo sapiens

Type:

Expression profiling by array, count, 2 disease state, 2 growth protocol sets

Platform:

GPL570

Series:

GSE16464

12 Samples

Download data: CEL, EXP

(Submitter supplied) Gray matter volume in the cerebral cortex has been consistently found to be decreased in patients with schizophrenia. The superior temporal gyrus (STG) is one of the cortical regions that exhibit the most pronounced volumetric reduction. This reduction is generally thought to reflect, at least in part, decreased number of synapses; the majority of these synapses are believed to be furnished by glutamatergic axon terminals onto the dendritic spines on pyramidal neurons. more...

Organism:

Homo sapiens

Type:

Expression profiling by array

Platform:

GPL1352

18 Samples

Download data: CEL, CHP

(Submitter supplied) Enhanced understanding of differential gene expression and biological pathways associated with distinct phases of intramembranous bone regeneration following femoral marrow ablation surgery will improve future advancements regarding osseointegration of joint replacement implants, biomaterials design, and bone tissue engineering. A rat femoral marrow ablation model was performed and genome-wide microarray data were obtained from samples at 1, 3, 5, 7, 10, 14, 28, and 56 days post-ablation, with intact bones serving as controls at Day 0. more...

Organism:

Rattus norvegicus

Type:

Expression profiling by array

Platform:

GPL1355

27 Samples

Download data: CEL

(Submitter supplied) Studies in Aotearoa New Zealand found higher bone mineral density and lower rate of hip fracture in people of Polynesian ancestry compared to Europeans. We hypothesised that differences in osteoblast gene expression contribute to the differences in bone properties between the two groups, and aimed to identify mechanisms that underlie the bone phenotype.

Organism:

Homo sapiens

Type:

Expression profiling by array

Platform:

GPL15207

20 Samples

Download data: CEL, CHP

(Submitter supplied) We have compared synovial biopsies from ankylosing spondylitis and undifferentiated spondylitis patients with healthy controls and osteoarthritis patients Objective: In spondylarthropies, whole-genome gene expression profiling studies have been limited to peripheral blood to date. By undertaking a study in knee synovial biopsies from spondylarthropy (SpA) and ankylosing spondylitis (AS) patients we aimed to identified joint-specific candidate genes and pathways. more...

Organism:

Homo sapiens

Type:

Expression profiling by array

Platform:

GPL6883

15 Samples

Download data: TXT

(Submitter supplied) We used a chicken RNA microarray to identify differentially expressed genes in order to compare two layer lines kept in a small group housing system Eurovent Deutsch.

Organism:

Gallus gallus

Type:

Expression profiling by array

Platform:

GPL18368

60 Samples

Download data: CEL, TXT

(Submitter supplied) Comparative analysis of gene expression profiles in newly developed housing systems is important to understand gene functions in chicken for adaptation and possible gene-environment interactions among layer lines. Therefore, the objective of this study was to characterize the molecular processes that are different among the two layer lines Lohmann Selected Leghorn (LSL) and Lohmann Brown (LB) using whole genome RNA expression profiles. more...

Organism:

Gallus gallus

Type:

Expression profiling by array

Platform:

GPL3213

60 Samples

Download data: CEL