GEO DataSets

(Submitter supplied) Using whole-genome Affymetrix microarrays (HG-U133A), we characterized the transcriptome profile of cultured human macrophages stimulated for 4 h with interleukin 1 (IL-1) or interleukin 6 (IL-6). We found that, in distinction to liver cells, IL-1 is much more potent than IL-6 in modifying macrophage gene expression, although considerable heterogeneity in response of macrophages deriving from individual blood donors was observed. more...

Organism:

Homo sapiens

Type:

Expression profiling by array

Dataset:

GDS3005

Platform:

GPL96

15 Samples

Download data: CEL

Analysis of cultured monocyte-derived macrophages (MDM) stimulated with interleukin (IL) 1 or IL-6 for 4 hours. Cytokines IL-1 and IL-6 are not only produced by macrophages but also affect the cells in an autocrine manner. Results provide insight into IL-1 and IL-6 responsive genes in MDMs.

Organism:

Homo sapiens

Type:

Expression profiling by array, count, 3 agent sets

Platform:

GPL96

Series:

GSE8515

15 Samples

Download data: CEL

(Submitter supplied) Endothelial cells comprise a key component of the inflammatory response. HUVEC (human umbilical vein endothelial cells) were stimulated with interleukin-1 for 0, 0.5, 1, 2.5 and 6 hours, and analyzed using Affymetrix U133A microarrays, in order to obtain a comprehensive overview of the immediate early to early gene expression profiles. HUVEC were isolated and cultured on gelatine-coated cell culture dishes in M199 medium supplemented with 20% FCS, antibiotics, ECGS (endothelial cell growth supplement) and Heparin as described by Zhang et al., Blood 1996;88:3880-3886. more...

Organism:

Homo sapiens

Type:

Expression profiling by array

Dataset:

GDS649

Platform:

GPL96

5 Samples

Download data: CEL

Temporal analysis of human umbilical vein endothelial cell (HUVEC) inflammatory response to interleukin-1 (IL-1) stimulation for 0, 0.5, 1, 2.5, and 6 hours. Immediate-early to early gene expression program characterized.

Organism:

Homo sapiens

Type:

Expression profiling by array, count, 5 time sets

Platform:

GPL96

Series:

GSE973

5 Samples

Download data: CEL

(Submitter supplied) Paeony root has long been used for its anti-inflammatory effects. In this study, the effects of albiflorin, paeoniflorin, and paeonol, compounds from paeony root, on gene expression profiles were examined in macrophages. The RAW264.7 macrophages were treated with LPS in the presence or absence of albiflorin, paeoniflorin, or paeonol. Global mRNA expression levels were detected by using an oligonucleotide microarray platform covering the mouse whole genome. more...

Organism:

Mus musculus

Type:

Expression profiling by array

Platform:

GPL2995

33 Samples

Download data: TXT

(Submitter supplied) Aged humans and rodents are susceptible to infection with Streptococcus pneumoniae bacteria as a result of an inability to make antibodies to capsular polysaccharides. This is partly a result of decreased production of proinflammatory cytokines and increased production of interleukin (IL)-10 by macrophages (Mphi) from aged mice. To understand the molecular basis of cytokine dysregulation in aged mouse Mphi, a microarray analysis was performed on RNA from resting and lipopolysaccharide (LPS)-stimulated Mphi from aged and control mice using the Affymetrix Mouse Genome 430 2.0 gene chip. more...

Organism:

Mus musculus

Type:

Expression profiling by array

Platform:

GPL1261

12 Samples

Download data: CEL

(Submitter supplied) The aim of the experiment was to determine the effect of cyclic stretch-relaxation ("stretch") on gene expression patterns in normal diploid human bladder smooth muscle cells. Cells plated on silicone elastomer bottomed 6-well culture dishes were grown to ~80% confluence, serum-depleted for 48h and subjected to cyclic stretch-relaxation at 20% elongation for 4h. Cells seeded in stretch plates but not subjected to stretch served as controls. more...

Organism:

Homo sapiens

Type:

Expression profiling by array

Dataset:

GDS906

Platform:

GPL96

8 Samples

Download data

Expression profiling of cultured bladder smooth muscle cells subjected to repetitive mechanical stimulation for 4 hours. Chronic overdistension results in bladder wall thickening, associated with loss of muscle contractility. Results identify genes whose expression is altered by mechanical stimuli.

Organism:

Homo sapiens

Type:

Expression profiling by array, count, 2 protocol sets

Platform:

GPL96

Series:

GSE1595

8 Samples

Download data

(Submitter supplied) HMG-CoA reductase inhibitors, statins, have beneficial vascular effects beyond their cholesterol-lowering action. These pleiotropic effects include an anti-inflammatory effect on macrophages. Since macrophages play a central role in atherogenesis, we further characterized the effects on peripheral blood monocyte-macrophages (HPBM). Using Affymetrix gene chip analysis of simvastatin-treated HPBM, we found that simvastatin treatment lead to the downregulation of the expression of many proinflammatory genes including several chemokines (e.g. more...

Organism:

Homo sapiens

Type:

Expression profiling by array

Platform:

GPL570

9 Samples

Download data: CEL

(Submitter supplied) Macrophages play a critical role in the pathogenesis of many diseases, including rheumatoid arthritis, inflammatory bowel disease and atherosclerosis. Monocytes recruited into tissues from peripheral blood differentiate into macrophages. There is limited data concerning the global changes in the expression of genes during monocyte to macrophage, and how the patterns of change identify the mechanism contributing to differentiation or macrophage function. more...

Organism:

Homo sapiens

Type:

Expression profiling by array

Dataset:

GDS3203

Platform:

GPL96

9 Samples

Download data: CEL

Analysis of monocytes following up to 168 hours of adherence-induced differentiation. Monocytes differentiate into macrophages, which are critical in the pathogenesis of many diseases. Results provide insight into the molecular mechanisms underlying monocyte to macrophage differentiation.

Organism:

Homo sapiens

Type:

Expression profiling by array, count, 2 cell type, 3 time sets

Platform:

GPL96

Series:

GSE8286

9 Samples

Download data: CEL

(Submitter supplied) Here we have used targeted RNA interference to suppress the expression of a number of key genes associated with IFN signalling in murine macrophages prior to activation. Genome-wide changes in transcript abundance caused by siRNA activity were measured using Affymetrix exon-arrays in the presence or absence of IFNγ stimulation. Transfection of murine bone-marrow derived macrophages (BMDMs) with non-targeting (control) siRNA and 11 sequence-specific siRNAs was performed using a cationic lipid transfection reagent (Lipofectamine2000) prior to stimulation with IFNγ. more...

Organism:

Mus musculus

Type:

Expression profiling by array

Platform:

GPL6096

90 Samples

Download data: CEL, CHP

(Submitter supplied) Premature birth continues to be a challenging pregnancy complication, and a body of literature indicates that inflammation can contribute to premature delivery by converting a receptive uterine environment to a hostile one. Cytokines have been demonstrated to provoke up-regulation of inflammatory genes (e.g. interleukin-1, 6, and 8, tumor necrosis factor-alpha, cyclooxygenase-2, and microsomal prostaglandin E synthase-1). more...

Organism:

Homo sapiens

Type:

Expression profiling by array

Dataset:

GDS4595

Platform:

GPL571

9 Samples

Download data: CEL

Analysis of monolayer cultures of amnion mesenchymal cells (AMC) following interleukin-1β challenge for up to 8 hrs. These AMCs represent an in vitro model system by which to study the inflammatory component of labor. Results provide insight into molecular response of AMCs to cytokine challenge.

Organism:

Homo sapiens

Type:

Expression profiling by array, transformed count, 2 agent, 3 time sets

Platform:

GPL571

Series:

GSE26315

9 Samples

Download data: CEL

(Submitter supplied) To investigate the time-dependent and coordinated sequence of inflammation-related events, and the dynamic features of macrophage polarisation/activation, we build and validated an in vitro model based on primary human monocytes

Organism:

Homo sapiens

Type:

Expression profiling by array

Platform:

GPL17180

60 Samples

Download data: CEL

(Submitter supplied) This dataset was created to study M-CSF dependent in vitro differentiation of human monocytes to macrophages as a model process to demonstrate that independent component analysis (ICA) is a useful tool to support and extend knowledge-based strategies and to identify complex regulatory networks or novel regulatory candidate genes. Keywords: M-CSF, Monocytes, Macrophages

Organism:

Homo sapiens

Type:

Expression profiling by array

Platforms:

GPL570 GPL96

14 Samples

Download data: CEL

(Submitter supplied) Polarization has been a useful concept for describing activated macrophage phenotypes and gene expression profiles. However, macrophage activation status within tumors and other settings are often inferred based on only a few markers. Complicating matters for relevance to human biology, many of the best studied macrophage activation markers have been best characterized in mice and sometimes are not similarly regulated in human macrophages. more...

Organism:

Homo sapiens

Type:

Expression profiling by array

Platform:

GPL10558

36 Samples

Download data: TXT

(Submitter supplied) External stimulations of cells by hormones, growth factors or cytokines activate signal transduction pathways that subsequently induce a rearrangement of cellular gene expression. The representation and analysis of changes in the gene response is complicated, and essentially consists of multiple layered temporal responses. In such situations, matrix factorization techniques may provide efficient tools for the detailed temporal analysis. more...

Organism:

Mus musculus

Type:

Expression profiling by array

Platform:

GPL1261

12 Samples

Download data: CEL