GEO DataSets

(Submitter supplied) We have determined the gene expression profile induced by 17 alpha-ethynyl estradiol (EE) in Ishikawa cells, a human uterine-derived estrogen-sensitive cell line, at various doses (1 pM, 100 pM, 10 nM, and 1 microM) and time points (8, 24, and 48 h). The transcript profiles were compared between treatment groups and controls (vehicle-treated) using high-density oligonucleotide arrays to determine the expression level of approximately 38,500 human genes. more...

Organism:

Homo sapiens

Type:

Expression profiling by array

Platform:

GPL570

75 Samples

Download data: CEL, CHP

(Submitter supplied) This study provides a comprehensive evaluation of changes in gene expression during treatment with Genistein in vitro. Ishikawa cells were maintained and grown (to confluency or at a desired cell density between 500,000 - 1 million cells/mL) in DMEM/F-12 Medium (supplemented with 10% Fetal Bovine Serum + 1X penicillin/streptomycin) in xenoestrogen-free Corning plasticware. Cells were gently washed in warm PBS and transferred to phenol red-free DMEM/F-12 Medium (supplemented with 10% Charcoal-Stripped Fetal Bovine Serum + 1X penicillin/streptomycin) in Corning Cell Culture Cluster wells overnight and then challenged with 10pM (very low, vL), 1 nM (low, L), 10nM (high, H), and 1uM (very high, vH) levels of Genistein. more...

Organism:

Homo sapiens

Type:

Expression profiling by array

Platform:

GPL570

60 Samples

Download data: CEL, CHP

(Submitter supplied) This study provides a comprehensive evaluation of changes in gene expression during treatment with Bisphenol A in vitro. Ishikawa cells were maintained and grown (to confluency or at a desired cell density between 500,000 - 1 million cells/mL) in DMEM/F-12 Medium (supplemented with 10% Fetal Bovine Serum + 1X penicillin/streptomycin) in xenoestrogen-free Corning plasticware. Cells were gently washed in warm PBS and transferred to phenol red-free DMEM/F-12 Medium (supplemented with 10% Charcoal-Stripped Fetal Bovine Serum + 1X penicillin/streptomycin) in Corning Cell Culture Cluster wells overnight and then challenged with 1pM (very low, vL), 100pM (low, L), 10nM (high, H), and 1uM (very high, vH) levels of BPA. more...

Organism:

Homo sapiens

Type:

Expression profiling by array

Dataset:

GDS4926

Platform:

GPL570

60 Samples

Download data: CEL, CHP

Temporal analysis of Ishikawa endometrial cells treated with various concentrations of bishpenol A (BPA). BPA is a very weak environmental estrogen given its low estrogen receptor affinity. Ishikawa cells represent a model to study the endometrial epithelium response to estrogen exposure in vitro.

Organism:

Homo sapiens

Type:

Expression profiling by array, count, 2 agent, 5 dose, 3 time sets

Platform:

GPL570

Series:

GSE17624

60 Samples

Download data: CEL, CHP

(Submitter supplied) Effects of estrogen on global gene expression: identification of novel targets of estrogen action. Keywords: other

Organism:

Homo sapiens

Type:

Expression profiling by SAGE

Platform:

GPL4

4 Samples

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(Submitter supplied) The alkylating agent N-methyl-N’-nitro-N-nitrosoguanidine (MNNG) induces cellular DNA damages and other comprehensive alterations that lead to chromosomal aberrations, mutations, tumor initiations, and cell death. However, the molecular mechanism of MNNG-induced cellular stress remains unclear.We have genome-wide analyzed early transcriptional responses of human FL amnion epithelial cells after exposure to three relatively low doses of MNNG (0.2, 1.0, and 10.0µM),and differential gene expression profiles were obtained 4 h after exposure using oligonucleotide microarrays followed by validation with quantitative real-time RT-PCR. more...

Organism:

Homo sapiens

Type:

Expression profiling by array

Platforms:

GPL96 GPL97

16 Samples

Download data: CEL, EXP

(Submitter supplied) Immature, ovariectomized C57BL/6 mice were treated with sesame oil (vehicle) or with 0.1 mg/kg 17-alpha-ethynylestradiol (EE) for the length of time indicated. Mice received one dose, except for the 3x24hr samples, which received 3 consecutive daily doses and were sacrificed 24hr after the final dose. Two replicates of each sample are provided. In addition, one time=0 sample is included. Keywords = uterus estrogen time mouse Keywords: time-course

Organism:

Mus musculus

Type:

Expression profiling by array

Dataset:

GDS285

Platform:

GPL75

21 Samples

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Temporal examination of ethynyl estradiol (EE) in uterus of immature, ovariectomized C57BL/6. 0.1 mg/kg EE or vehicle administered and uteri harvested at 0, 2, 8, 12, or 24 hours, or three daily doses administered and uteri harvested at 72 hours.

Organism:

Mus musculus

Type:

Expression profiling by array, count, 3 agent, 6 time sets

Platform:

GPL75

Series:

GSE280

21 Samples

Download data

(Submitter supplied) Previously, we described a mouse model where the well-known reproductive carcinogen, diethylstilbestrol (DES), caused uterine adenocarcinoma following neonatal treatment. Tumor incidence was dose-dependent reaching >90% by 18 mo. following 1000 µg/kg/day of DES. These tumors followed the initiation/promotion model of hormonal carcinogenesis with developmental exposure as the initiator, and exposure to ovarian hormones at puberty as the promoter. more...

Organism:

Mus musculus

Type:

Expression profiling by array

Dataset:

GDS2924

Platform:

GPL891

6 Samples

Download data: TIFF, TXT

Analysis of uteri of prepubertal animals treated with diethylstilbestrol (DES) at doses of 1, 10, or 1000 ug/kg/day. DES treatment of neonates results in the development of uterine adenocarcinomas. Results provide insight into the mechanisms underlying the initiation of DES-induced carcinogenesis.

Organism:

Mus musculus

Type:

Expression profiling by array, log2 ratio, 3 dose sets

Platform:

GPL891

Series:

GSE5825

6 Samples

Download data: TIFF, TXT

(Submitter supplied) Blackcurrants (Ribes nigrum L., Grossulariaceae) have a high content of anthocyanin polyphenols and these have been shown to have beneficial effects on health, owing to their antioxidant and anti-carcinogenic properties. This study analyzed the constituents of blackcurrant extract (BCE) and investigated its potential phytoestrogenic effects using a breast cancer cell line (MCF-7) overexpressing the estrogen receptor (ER) α. more...

Organism:

Homo sapiens

Type:

Expression profiling by array

Platform:

GPL17077

2 Samples

Download data: TXT

(Submitter supplied) To test wether endocrine disruption is detectable in zebrafish at the transcriptome level, we exposed newly fertilized zebrafish embryos to low doses of genistein (EC10 and EC20) for 48 hours.

Organism:

Danio rerio

Type:

Expression profiling by array

Platform:

GPL6457

16 Samples

Download data: TXT

(Submitter supplied) To obtain comprehensive information on 17beta-estradiol (E2) sensitivity of genes that are inducible or suppressible by this hormone, we designed a method that determines ligand sensitivities of large numbers of genes using DNA microarray and a set of simple Perl computer scripts implementing the standard metric statistics, and employed it to characterize effects of low (0-100 pM) concentrations of E2 on the transcriptome profile of MCF7/BUS human breast cancer cells, whose E2 dose-dependent growth curve saturated with 100 pM E2. more...

Organism:

Homo sapiens

Type:

Expression profiling by array

Datasets:

GDS2323 GDS2324

Platform:

GPL96

34 Samples

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Analysis of breast cancer MCF7/BUS cells treated with 17beta-estradiol (E2) at concentrations up to 100 pM. Results compared to that from an experiment examining the response to hormone starvation (GDS2323). Results provide insight into the E2 sensitivity of E2 inducible or suppressible genes.

Organism:

Homo sapiens

Type:

Expression profiling by array, count, 5 dose sets

Platform:

GPL96

Series:

GSE4668

25 Samples

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Analysis of breast cancer MCF7/BUS cells starved of estrogen for up to 2 days. Results compared to that from an experiment (GDS2324) examining the response to low concentrations of 17beta-estradiol (E2). Results provide insight into the E2 sensitivity of E2 inducible or suppressible genes.

Organism:

Homo sapiens

Type:

Expression profiling by array, count, 3 time sets

Platform:

GPL96

Series:

GSE4668

9 Samples

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(Submitter supplied) This SuperSeries is composed of the SubSeries listed below.

Organism:

Homo sapiens

Type:

Expression profiling by array

Platform:

GPL13667

1402 Samples

Download data: CEL, CHP

(Submitter supplied) This study provides an evaluation of changes in gene expression associated with treating human HEPG2 cells with 34 different chemical compounds. Gene expression experiments studies were performed in triplicate (n=3) with the cells for each replicate treated and harvested on separate days.

Organism:

Homo sapiens

Type:

Expression profiling by array

Platform:

GPL13667

348 Samples

Download data: CEL, CHP

(Submitter supplied) This study provides an evaluation of changes in gene expression associated with treating human Ishikawa cells with 34 different chemical compounds. Gene expression experiments studies were performed in triplicate (n=3) with the cells for each replicate treated and harvested on separate days.

Organism:

Homo sapiens

Type:

Expression profiling by array

Platform:

GPL13667

363 Samples

Download data: CEL, CHP

(Submitter supplied) This study provides an evaluation of changes in gene expression associated with treating human MCF7 cells with 34 different chemical compounds. Gene expression experiments studies were performed in triplicate (n=3) with the cells for each replicate treated and harvested on separate days.

Organism:

Homo sapiens

Type:

Expression profiling by array

Platform:

GPL13667

360 Samples

Download data: CEL, CHP

(Submitter supplied) This study provides an evaluation of changes in gene expression associated with treating human HepaRG cells with 34 different chemical compounds. Gene expression experiments studies were performed in triplicate (n=3) with the cells for each replicate treated and harvested on separate days.

Organism:

Homo sapiens

Type:

Expression profiling by array

Platform:

GPL13667

331 Samples

Download data: CEL, CHP