GEO DataSets

(Submitter supplied) We focused on how Mycobacterium avium subsp. paratuberculosis influences the subsequent host response to investigate the host immunopathology accompanying the host anti-mycobacterial immune response during Mycobacterium avium subsp. paratuberculosis infection in spleen of mice.

Organism:

Mus musculus

Type:

Expression profiling by array

Platform:

GPL6887

12 Samples

Download data: TXT

(Submitter supplied) To provide novel insights into understanding the host-immune response during the different stages of progression of the disease, we performed gene expression profiling in Mycobacterium avium subsp. paratuberculosis infection in cattle.

Organism:

Bos taurus

Type:

Expression profiling by array

Platform:

GPL11649

12 Samples

Download data: TXT

(Submitter supplied) Background: Mycobacterium avium subspecies paratuberculosis (MPTb) is the causative agent of Johne’s disease, an intestinal disease of ruminants with major economic consequences. MPTb bacilli are phagocytosed by host macrophages upon exposure where they persist, resulting in lengthy subclinical phases of infection that can lead to immunopathology and disease dissemination. Consequently, analysis of the macrophage transcriptome in response to MPTb infection can provide valuable insights into the molecular mechanisms that underlie Johne’s disease. more...

Organism:

Bos taurus

Type:

Expression profiling by array

Dataset:

GDS4518

Platform:

GPL2112

48 Samples

Download data: CEL

Analysis of monocyte-derived macrophages isolated from Holstein-Friesian females in response to M. avium subsp. paratuberculosis (MPTb) infection in vitro for up to 24hrs. MPTb is the etiological agent of Johne's disease. Results provide insight into the molecular basis of the host immune response.

Organism:

Bos taurus

Type:

Expression profiling by array, transformed count, 2 infection, 4 time sets

Platform:

GPL2112

Series:

GSE35185

48 Samples

Download data: CEL

(Submitter supplied) Background: Johne’s disease is a chronic wasting disease caused by the bacterium Mycobacterium avium subspecies paratuberculosis (MAP). Johne’s disease is highly contagious and MAP infection in dairy cattle can eventually lead to death. With no available treatment for Johne’s disease, genetic selection and improvements in management practices could help reduce its prevalence. In a previous study, the gene coding interleukin-10 receptor subunit alpha (IL10Rα) was associated with Johne’s disease in dairy cattle. more...

Organism:

Bos taurus

Type:

Expression profiling by high throughput sequencing

Platform:

GPL19172

16 Samples

Download data: CSV

(Submitter supplied) To determine the initial responses of human THP-1 cells against MAP infection using single-cell RNA-seq analysis.

Organism:

Homo sapiens

Type:

Expression profiling by high throughput sequencing

Platform:

GPL24676

16 Samples

Download data: MTX, TSV

(Submitter supplied) Host-pathogen interactions of Mycobacterium avium subsp. paratuberculosis infection at transcriptomic level were investigated in an in vitro macrophage infection model by dual RNA-seq analysis.

Organism:

Homo sapiens; Mycobacterium avium subsp. paratuberculosis K-10

Type:

Expression profiling by high throughput sequencing

Platforms:

GPL24676 GPL32084 GPL32083

6 Samples

Download data: XLSX

(Submitter supplied) Mycobacterium avium subsp. paratuberculosis (MAP) causes Johne’s disease that is a chronic wasting disease in ruminants. Spread of MAP is mainly provoked by a long subclinical stage during which MAP is shed into the environment with feces; accordingly, detection of subclinical animals is very important to its control. Therefore, the current diagnostic methods are not suitable for detection of subclinical animals. more...

Organism:

Bos taurus

Type:

Non-coding RNA profiling by high throughput sequencing

Platform:

GPL26012

8 Samples

Download data: TXT

(Submitter supplied) The molecular regulatory mechanisms of host responses to Mycobacterium avium subsp. paratuberculosis (MAP) infection during the early subclinical stage are still not clear. In this study, surgically isolated ileal segments in newborn calves (n = 5) were used to establish in vivo MAP infection adjacent to an uninfected control intestinal compartment. RNA-seq was used to profile the whole transcriptome (mRNAs) and the microRNAome (miRNAs) of ileal tissues collected at one-month post-infection. more...

Organism:

Bos taurus

Type:

Expression profiling by high throughput sequencing; Non-coding RNA profiling by high throughput sequencing

Platform:

GPL15749

20 Samples

Download data: TXT

(Submitter supplied) Johne's disease, caused by infection with Mycobacterium avium subsp. paratuberculosis, (MAP), is a chronic intestinal disease of ruminants with serious economic consequences for cattle production in the United States and elsewhere. During infection, MAP bacilli are phagocytosed and subvert host macrophage processes, resulting in subclinical infections that can lead to immunopathology and dissemination of disease. more...

Organism:

Bos taurus

Type:

Expression profiling by high throughput sequencing

Platform:

GPL15749

35 Samples

Download data: TXT, XLSX

(Submitter supplied) Purpose: Understanding host-MAP interactions in initial stage of infection Methods: Native MAP and MDBK processed (4h) MAP were infected to bovine PBMCs. Total RNA after 24h and 72h were harvested. RNA-Seq was performed using total RNA of each group. Result: The gene expression patterns commonly activated in both groups suggest that Th17 activation is the main immunological response to MAP infection.

Organism:

Bos taurus

Type:

Expression profiling by high throughput sequencing

Platform:

GPL26012

12 Samples

Download data: XLS

(Submitter supplied) In this study, consistent gene expression changes were analysed to identify gene-to-gene interaction and functional pathway relationships associated with the MAP exposure outcomes of either a paucibacillary or a multibacillary disease form or sheep resilient to disease. The results suggest that although groups of genes are differentially changed in the MAP exposed sheep regardless of clinical outcome, there are distinct variations in gene expression patterns between the paucibacillary and multibacillary forms of disease and of particular note was the finding that MAP exposed animals with evidence of recovery or resilience respond to MAP exposure with a pattern of gene and molecular pathway interactions distinct from those animals that progress to clinical paratuberculosis.

Organism:

Bos taurus; Ovis aries

Type:

Expression profiling by array

Platform:

GPL2112

124 Samples

Download data: CEL

(Submitter supplied) This SuperSeries is composed of the SubSeries listed below.

Organism:

Mycobacterium avium subsp. paratuberculosis K-10; Mycobacterium avium subsp. paratuberculosis

Type:

Expression profiling by array

Platform:

GPL14590

2 Samples

Download data: GPR

(Submitter supplied) Transcriptional profile of Mycobacterium avium subspecies paratuberculosis in in vitro THP-1 (ATCC TIB-202) cell line infection, comparing bacteria grown in host medium and intracellular bacteria recovered from infected THP-1 cells.

Organism:

Mycobacterium avium subsp. paratuberculosis; Mycobacterium avium subsp. paratuberculosis K-10

Type:

Expression profiling by array

Platform:

GPL14590

1 Sample

Download data: GPR

(Submitter supplied) Transcriptional profile of Mycobacterium avium subspecies paratuberculosis in in vitro acid-nitrosative multistress, comparing untreated control cells and bacteria under multi-stress.

Organism:

Mycobacterium avium subsp. paratuberculosis; Mycobacterium avium subsp. paratuberculosis K-10

Type:

Expression profiling by array

Platform:

GPL14590

1 Sample

Download data: GPR

(Submitter supplied) In order to gain a better understanding of MAP infection in immature macrophages, high-throughput sequencing technology was used to perform an analysis of mRNAs profiles of bovine monocyte derived macrophages upon MAP infection. The total numbers of raw reads collected from the MAP-infected and control MDM cells were 93.6 and 102 million raw reads per library. After removing low-quality sequences, Adaptor sequences and sequences containing more than 5% N (N means that base information cannot be determined), 91.7 and 100 million clean reads were obtained respectively. more...

Organism:

Bos taurus

Type:

Expression profiling by high throughput sequencing; Non-coding RNA profiling by high throughput sequencing

Platform:

GPL26012

6 Samples

Download data: TXT

(Submitter supplied) Mycobacterium avium subspecies paratuberculosis (MAP) infection is established following the ingestion of bacteria, their penetration of the intestinal mucosa and subsequent events of the host-parasite interaction. These bacteria invade M cells, macrophages and are capable of resisting host defenses and multiply to reach very high numbers intracellular. Mycobactrium avium subsp. avium (MAA) is an antigenically and genetically similar organisms but is relatively nonpathogenic for cattle. more...

Organism:

Bos taurus

Type:

Expression profiling by array

Platform:

GPL2853

140 Samples

Download data: GPR

(Submitter supplied) Purpose: Since the peripheral blood (PB) is the most available physiological fluid for the detection of biomarkers, in the current we examine whether it recapitulates, at least in part, the transcriptome of the ileocecal valve (ICV), the primary site of MAP colonization. For this purpose, RNA-Seq was used to identify host genes differentially expressed (DE) in ICV and PB samples collected from PTB-infected animals with focal or diffuse lesions in gut tissues versus control animals. more...

Organism:

Bos taurus

Type:

Expression profiling by high throughput sequencing

Platform:

GPL23055

28 Samples

Download data: TXT

(Submitter supplied) This SuperSeries is composed of the SubSeries listed below.

Organism:

Homo sapiens

Type:

Expression profiling by high throughput sequencing; Non-coding RNA profiling by high throughput sequencing

Platforms:

GPL15520 GPL11154

52 Samples

Download data

(Submitter supplied) Mycobacterium abscessus is an emerging pathogen causing pulmonary infections in those with inflammatory lung disorders, such as Cystic Fibrosis (CF), and is associated with the highest fatality rate among rapidly growing mycobacteria (RGM). Phenotypically, MAB manifests as either a Smooth (MAB-S) or a Rough (MAB-R) morphotype, which differ in their levels of cell wall glycopeptidolipids (GPLs) and in their pathogenicity in vivo. more...

Organism:

Homo sapiens

Type:

Expression profiling by high throughput sequencing

Platforms:

GPL15520 GPL11154

27 Samples

Download data: XLS