GEO DataSets

(Submitter supplied) Cell-based models of many neurological and psychiatric diseases, established by reprogramming patient somatic cells into human induced pluripotent stem cells (hiPSCs), have now been reported. While numerous reports have demonstrated that neuronal cells differentiated from hiPSCs are electrophysiologically active mature neurons, the “age” of these cells relative to cells in the human brain remains unresolved. more...

Organism:

Homo sapiens

Type:

Expression profiling by high throughput sequencing

Platform:

GPL16791

11 Samples

Download data: TXT

(Submitter supplied) To follow-up findings that miR-9 was abundantly expressed in control NPCs, significantly down-regulated in a subset of SZ NPCs, and that miR-9 levels/activity, neural migration and diagnosis were strongly correlated, we tested the effect of manipulating miR-9 at cellular, proteomic and transcriptomic levels. Unexpectedly, proteomic- and RNAseq-based analysis revealed that these effects were mediated primarily by small changes in expression of indirect miR-9 targets, rather than large changes in direct miR-9 targets; these indirect targets are enriched for migration-associated genes. more...

Organism:

Homo sapiens

Type:

Expression profiling by high throughput sequencing

Platform:

GPL16791

16 Samples

Download data: TXT

(Submitter supplied) This SuperSeries is composed of the SubSeries listed below.

Organism:

Homo sapiens

Type:

Expression profiling by high throughput sequencing

Platform:

GPL16791

39 Samples

Download data

(Submitter supplied) Cell-based models of many neurological and psychiatric diseases, established by reprogramming patient somatic cells into human induced pluripotent stem cells (hiPSCs), have now been reported. While numerous reports have demonstrated that neuronal cells differentiated from hiPSCs are electrophysiologically active mature neurons, the “age” of these cells relative to cells in the human brain remains unresolved. more...

Organism:

Homo sapiens

Type:

Expression profiling by high throughput sequencing

Platform:

GPL16791

12 Samples

Download data: TXT

(Submitter supplied) We are using induced pluripotent stem cell (iPSC) technology to study neuropsychiatric disorders associated with 22q11.2 microdeletions (del), the most common known schizophrenia (SZ) -associated genetic factor. Several genes in the deleted region have been implicated; one of the more promising candidates is DGCR8, which codes for a protein involved in microRNA (miRNA) biogenesis. We carried out miRNA expression profiling (miRNA-seq) on neurons generated from iPSCs derived from controls and SZ patients with 22q11.2 del.

Organism:

Homo sapiens

Type:

Non-coding RNA profiling by high throughput sequencing

Platform:

GPL11154

16 Samples

Download data: XLS

(Submitter supplied) Cell-based models of many neurological and psychiatric diseases, established by reprogramming patient somatic cells into human induced pluripotent stem cells (hiPSCs), have now been reported. While numerous reports have demonstrated that neuronal cells differentiated from hiPSCs are electrophysiologically active mature neurons, the “age” of these cells relative to cells in the human brain remains unresolved. more...

Organism:

Homo sapiens

Type:

Expression profiling by array

Platform:

GPL6244

24 Samples

Download data: CEL, TXT

(Submitter supplied) In the largest published genome-wide association study (GWAS) of schizophrenia to date (PGC1), the most significant association (P = 1.6 X 10-11) was observed at an intronic variant (rs1625579) within the MIR137 host gene (MIR137HG). In this study we have performed genome-wide RNA profiling of a human foetal neural progenitor cell line following miR-137 manipulation, in order to identify gene expression changes through which genetic variation at the MIR137HG locus could confer susceptibility to schizophrenia.

Organism:

Homo sapiens

Type:

Expression profiling by array

Platform:

GPL10558

12 Samples

Download data: TXT

(Submitter supplied) MicroRNAs (miRNA) play an essential role in the regulation of gene expression, influence signaling networks responsible for several cellular processes like differentiation of pluripotent stem cells. Despite several studies on the neurogenesis process, no global analysis of microRNA expression during differentiation of induced pluripotent stem cells (iPSC) to neuronal stem cells (NSC) has been done. Therefore we compared the profile of microRNA expression in iPSC lines and in NSC lines derived from them, using microarray-based analysis. Two different protocols for NSC formation were used: direct and two-step via neural rosette formation. We confirmed the new associations of previously described miRNAs in regulation of NSC differentiation from iPSC. We discovered upregulation of miR-10 family, miR-30 family and miR-9 family and downregulation of miR-302 and miR-515 family. Moreover we showed that miR-10 family play a crucial role in the negative regulation of genes expression belonging to signaling pathways involved in neural differentiation: WNT signaling pathway, focal adhesion, signaling pathways regulating pluripotency of stem cells.

Organism:

synthetic construct; Homo sapiens

Type:

Non-coding RNA profiling by array

Platform:

GPL19117

8 Samples

Download data: CEL

(Submitter supplied) We report the data generated upon knockdown of TCF4 in hiPSC-derived neural progenitor cells and glutamatergic neurons.

Organism:

Homo sapiens

Type:

Expression profiling by high throughput sequencing

Platform:

GPL16791

23 Samples

Download data: TXT

(Submitter supplied) CHD8 (chromodomain helicase DNA binding protein 8), which codes for a member of the CHD family of ATP-dependent chromatin-remodeling factors, is the most commonly mutated gene in autism spectrum disorders (ASD) identified in exome-sequencing studies. Loss of function mutations in the gene have also been found in schizophrenia (SZ) and intellectual disabilities, and affects cancer cell proliferation. more...

Organism:

Homo sapiens

Type:

Expression profiling by high throughput sequencing

Platform:

GPL16791

6 Samples

Download data: TXT

(Submitter supplied) Whereas highly penetrant variants have proven well-suited to human induced pluripotent stem cell (hiPSC)-based models, the power of hiPSC-based studies to resolve the much smaller effects of common variants within the size of cohorts that can be realistically assembled remains uncertain. In developing a large case/control schizophrenia (SZ) hiPSC-derived cohort of neural progenitor cells and neurons, we identified and accounted for a variety of technical and biological sources of variation. more...

Organism:

Homo sapiens

Type:

Expression profiling by high throughput sequencing

Platform:

GPL16791

94 Samples

Download data: CSV

(Submitter supplied) This SuperSeries is composed of the SubSeries listed below.

Organism:

Homo sapiens

Type:

Expression profiling by array; Expression profiling by high throughput sequencing

Platforms:

GPL25480 GPL16791

5718 Samples

Download data

(Submitter supplied) Integration of in silico and in vitro approaches to design and conduct transcriptomic drug screening in patient-derived neural cells, in order to survey novel pathologies and points of intervention in schizophrenia.

Organism:

Homo sapiens

Type:

Expression profiling by high throughput sequencing

Platform:

GPL16791

44 Samples

Download data: TXT

(Submitter supplied) Integration of in silico and in vitro approaches to design and conduct transcriptomic drug screening in patient-derived neural cells, in order to survey novel pathologies and points of intervention in schizophrenia.

Organism:

Homo sapiens

Type:

Expression profiling by array

Platform:

GPL25480

380 Samples

Download data

(Submitter supplied) Integration of in silico and in vitro approaches to design and conduct transcriptomic drug screening in patient-derived neural cells, in order to survey novel pathologies and points of intervention in schizophrenia.

Organism:

Homo sapiens

Type:

Expression profiling by array

Platform:

GPL25480

380 Samples

Download data

(Submitter supplied) Integration of in silico and in vitro approaches to design and conduct transcriptomic drug screening in patient-derived neural cells, in order to survey novel pathologies and points of intervention in schizophrenia.

Organism:

Homo sapiens

Type:

Expression profiling by array

Platform:

GPL25480

380 Samples

Download data

(Submitter supplied) Integration of in silico and in vitro approaches to design and conduct transcriptomic drug screening in patient-derived neural cells, in order to survey novel pathologies and points of intervention in schizophrenia.

Organism:

Homo sapiens

Type:

Expression profiling by array

Platform:

GPL25480

380 Samples

Download data

(Submitter supplied) Integration of in silico and in vitro approaches to design and conduct transcriptomic drug screening in patient-derived neural cells, in order to survey novel pathologies and points of intervention in schizophrenia.

Organism:

Homo sapiens

Type:

Expression profiling by array

Platform:

GPL25480

380 Samples

Download data

(Submitter supplied) Integration of in silico and in vitro approaches to design and conduct transcriptomic drug screening in patient-derived neural cells, in order to survey novel pathologies and points of intervention in schizophrenia.

Organism:

Homo sapiens

Type:

Expression profiling by array

Platform:

GPL25480

378 Samples

Download data

(Submitter supplied) Integration of in silico and in vitro approaches to design and conduct transcriptomic drug screening in patient-derived neural cells, in order to survey novel pathologies and points of intervention in schizophrenia.

Organism:

Homo sapiens

Type:

Expression profiling by array

Platform:

GPL25480

378 Samples

Download data