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Links from GEO DataSets

Items: 20

1.

Small RNA and degradome sequencing reveal complex roles of miRNAs and their targets in the developing grains of wheat (Triticum aestivum L.)

(Submitter supplied) This SuperSeries is composed of the SubSeries listed below.
Organism:
Triticum aestivum
Type:
Non-coding RNA profiling by high throughput sequencing; Other
Platforms:
GPL17701 GPL15632
6 Samples
Download data: TXT
Series
Accession:
GSE65799
ID:
200065799
2.

Degradome sequencing analysis in developing wheat grains

(Submitter supplied) We aimed to identify targets of miRNAs during wheat grain development by using degradome sequencing approach. Two degradome libraries were constructed from wheat grains.
Organism:
Triticum aestivum
Type:
Other
Platform:
GPL15632
2 Samples
Download data: TXT
Series
Accession:
GSE65798
ID:
200065798
3.

Expression profiling of small RNAs in developing wheat grains

(Submitter supplied) We aimed to identify differentially expressed miRNAs during wheat grain development by using high-throughput sequencing approach. Four small RNA libraries were constructed from wheat grains collected at 7, 14, 21 and 28 days post anthesis (DPA). A total of 165 known miRNAs and 37 novel miRNAs were identified in four small RNA libraries. Moreover, a miRNA-like long hairpin locus was first identified to produce 21~22-nt phased siRNAs. more...
Organism:
Triticum aestivum
Type:
Non-coding RNA profiling by high throughput sequencing
Platform:
GPL17701
4 Samples
Download data: TXT
Series
Accession:
GSE65797
ID:
200065797
4.

Identification of miRNAs and its targets using high-throughput sequencing and degradome analysis in male and female Asparagus officinalis.

(Submitter supplied) Two independent small RNA (sRNA) libraries from male and female asparagus plants were sequenced, generating 4.13 and 5.88 million final cleaned reads, respectively. A total of 154 conserved miRNA belonging to 26 families, and 40 novel miRNA candidates that seemed to be specific to asparagus were identified, among them, 63 miRNAs exhibited significant differential expression between male and female plants, and 36 target mRNAs representing 44 conserved and fournovel miRNA in asparagus by high-throughput degradome sequencing analysis.
Organism:
Asparagus officinalis
Type:
Non-coding RNA profiling by high throughput sequencing; Other
Platform:
GPL20875
3 Samples
Download data: XLS
Series
Accession:
GSE72594
ID:
200072594
5.

Genome-wide profiling of miRNAs and their target genes during somatic embryogenesis in cotton by small RNA and degradome sequencing

(Submitter supplied) MicroRNAs (miRNAs) are endogenous non-coding ~21 nucleotide (nt) RNAs that regulate gene expression at transcriptional and post-transcriptional levels in plants and animals. They play an important role in development, abiotic stress responses or pathogen responses. miRNAs with their related target genes have been widely studied in model plants,and increasing studies have been performed on some crops,however, the number of identified miRNAs in cotton was limited, and global identification of related targets through degradome sequencing has not been developed previously. more...
Organism:
Gossypium hirsutum
Type:
Expression profiling by high throughput sequencing; Non-coding RNA profiling by high throughput sequencing
Platform:
GPL16101
4 Samples
Download data: TXT
Series
Accession:
GSE41132
ID:
200041132
6.

High-throughput sequencing and degradome analysis reveal two distinct evolutionary patterns of Cercis gigantea microRNAs and their targets

(Submitter supplied) In this study, C. gigantea miRNAs and their target genes were investigated by extracting RNA from young roots, tender stems, young leaves, and flower buds of C. gigantea to establish a small RNA (sRNA) library and a degradome library to further sequence. This study identified 194 known miRNAs belonging to 52 miRNA families and 23 novel miRNAs. Among the miRNA families, 158 miRNAs from 27 miRNA families were highly conserved and existed in a plurality of plants. more...
Organism:
Cercis gigantea (nom. inval.)
Type:
Non-coding RNA profiling by high throughput sequencing; Other
Platforms:
GPL19878 GPL19879
2 Samples
Download data: TXT
Series
Accession:
GSE66754
ID:
200066754
7.

Uncovering small RNA-mediated responses to cold stress in a wheat thermosensitive genic male sterile line by deep sequencing (Degradome-Seq)

(Submitter supplied) The male sterility of thermosensitive genic male sterile (TGMS) lines of wheat (Triticum aestivum) is strictly controlled by temperature. The early phase of anther development is especially susceptible to cold stress. MicroRNAs (miRNA) play an important role in plant development and in responses to environmental stress. In this study, deep sequencing of small RNA (smRNA) libraries obtained from spike tissues of the TGMS line under cold and control conditions identified a total of 81 unique miRNA sequences from 30 families, and trans-acting small interfering RNAs (tasiRNAs) derived from two TAS3 genes. more...
Organism:
Triticum aestivum
Type:
Expression profiling by high throughput sequencing
Platform:
GPL10467
2 Samples
Download data: TXT
Series
Accession:
GSE37134
ID:
200037134
8.

Uncovering small RNA-mediated responses to cold stress in a wheat thermosensitive genic male sterile line by deep sequencing

(Submitter supplied) The male sterility of thermosensitive genic male sterile (TGMS) lines of wheat (Triticum aestivum) is strictly controlled by temperature. The early phase of anther development is especially susceptible to cold stress. MicroRNAs (miRNA) play an important role in plant development and in responses to environmental stress. In this study, deep sequencing of small RNA (smRNA) libraries obtained from spike tissues of the TGMS line under cold and control conditions identified a total of 81 unique miRNA sequences from 30 families, and trans-acting small interfering RNAs (tasiRNAs) derived from two TAS3 genes. more...
Organism:
Triticum aestivum
Type:
Non-coding RNA profiling by high throughput sequencing
Platform:
GPL10467
7 Samples
Download data: TXT
Series
Accession:
GSE36867
ID:
200036867
9.

The miRNAs and their targets in cucumber shoot apexes in response to temperature and photoperiod environments

(Submitter supplied) The cucumber is one of the most important vegetables worldwide and is used as a research model for study of phloem transport, sex determination and temperature-photoperiod physiology. The shoot apex is the most important plant tissue in which the cell fate and organ meristems have been determined. In this study, a series of whole-genome small RNA, degradome and transcriptome analyses were performed on cucumber shoot apical tissues treated with high vs. more...
Organism:
Cucumis sativus
Type:
Expression profiling by high throughput sequencing; Non-coding RNA profiling by high throughput sequencing; Other
Platform:
GPL16310
25 Samples
Download data: FA, TXT
Series
Accession:
GSE117867
ID:
200117867
10.

Multi-omics analysis of small RNA, transcriptome and degradome in T. turgidum – regulatory networks of grain development and abiotic stress response

(Submitter supplied) Crop reproduction is highly sensitive to water-deficit and heat stress. The molecular networks of stress adaptation and grain development in tetraploid wheat (T. turgidum durum) are not well understood. Small RNAs (sRNAs) are important epigenetic regulators connecting the transcriptional and post-transcriptional regulatory networks. This study presents the first multi-omics analysis of the sRNAome, transcriptome and degradome in T. more...
Organism:
Triticum turgidum subsp. durum
Type:
Expression profiling by high throughput sequencing; Non-coding RNA profiling by high throughput sequencing; Other
Platforms:
GPL28728 GPL20257
134 Samples
Download data: XLSX
Series
Accession:
GSE153932
ID:
200153932
11.

Identification and characterization of cold responsive microRNAs in tea plant (Camellia sinensis) based on high-throughput sequencing and their targets using degradome analysis

(Submitter supplied) This SuperSeries is composed of the SubSeries listed below.
Organism:
Embryophyta; Camellia sinensis
Type:
Non-coding RNA profiling by array; Non-coding RNA profiling by high throughput sequencing
Platforms:
GPL18106 GPL19224
11 Samples
Download data: TXT, XLSX
Series
Accession:
GSE61719
ID:
200061719
12.

Identification and characterization of cold-responsive microRNAs in tea plant (Camellia sinensis) and their targets using high-throughput sequencing and degradome analysis [miRNA-seq]

(Submitter supplied) We discovered 31 up-regulated miRNAs and 43 down-regulated miRNAs in ‘Yingshuang’, and 46 up-regulated miRNA and 45 down-regulated miRNAs in ‘Baiye 1’ in response to cold stress, respectively. A total of 763 related target genes were detected by degradome sequencing. The RLM-5’RACE procedure was successfully used to map the cleavage sites in six target genes of C. sinensis. These findings reveal important information about the regulatory mechanism of miRNAs in C. more...
Organism:
Camellia sinensis
Type:
Non-coding RNA profiling by high throughput sequencing
Platform:
GPL19224
3 Samples
Download data: XLSX
Series
Accession:
GSE61718
ID:
200061718
13.

Identification and characterization of cold responsive microRNAs in tea plant (Camellia sinensis) based on high-throughput sequencing and their targets using degradome analysis [microarray]

(Submitter supplied) Solexa sequencing technology was used to perform high throughput sequencing of the small RNA library from the cold treatment of tea leaves. Subsequently, aligning these sequencing date with plant known miRNAs, we characterized 112 C. sinensis conserved miRNAs. In addition, 215 potential candidate miRNAs were found; among them, 131 candidates with star sequence were chosen as novel miRNAs. There are both congruously and differently regulated miRNAs, and line-specific miRNAs were identified by microarray-based hybridization in response to cold stress. more...
Organism:
Camellia sinensis; Embryophyta
Type:
Non-coding RNA profiling by array
Platform:
GPL18106
8 Samples
Download data: TXT
Series
Accession:
GSE53632
ID:
200053632
14.

Identification of miRNAs and their target genes in developing maize ears by deep sequencing

(Submitter supplied) In plants, MicroRNAs (miRNAs) are a new class of endogenous small RNAs that play essential regulatory roles in plant growth, development and stress response. Extensive studies of miRNAs have been performed in model plants such as rice, Arabidopsis thaliana and other plants. However, the number of miRNAs discovered in maize is relatively low and little is known about miRNAs involved in the four stages during maize ear development. more...
Organism:
Zea mays
Type:
Expression profiling by high throughput sequencing; Non-coding RNA profiling by high throughput sequencing
Platform:
GPL9361
5 Samples
Download data: TXT
Series
Accession:
GSE47837
ID:
200047837
15.

MicroRNA-mediated regulation of gene expression in the response of rice plants to fungal elicitors

(Submitter supplied) This SuperSeries is composed of the SubSeries listed below.
Organism:
Oryza sativa
Type:
Other; Non-coding RNA profiling by high throughput sequencing
Platform:
GPL13160
28 Samples
Download data: TXT
Series
Accession:
GSE66611
ID:
200066611
16.

MicroRNA-mediated regulation of gene expression in the response of rice plants to fungal elicitors [degradome]

(Submitter supplied) Elicitor-responsive miRNAs and their targets in rice
Organism:
Oryza sativa
Type:
Other
Platform:
GPL13160
4 Samples
Download data: TXT
Series
Accession:
GSE66610
ID:
200066610
17.

MicroRNA-mediated regulation of gene expression in the response of rice plants to fungal elicitors [miRNA-Seq]

(Submitter supplied) Elicitor-responsive miRNAs and their targets in rice
Organism:
Oryza sativa
Type:
Non-coding RNA profiling by high throughput sequencing
Platform:
GPL13160
24 Samples
Download data: TXT
Series
Accession:
GSE66609
ID:
200066609
18.

Identification of miRNAs and their target genes in developing seeds of soybean by deep sequencing

(Submitter supplied) In our study, small RNA library and degradome library were constructed from developing soybean seeds for deep sequencing. We identified 26 new miRNAs in soybean by bioinformatic analysis, and further confirmed their expression by stem-loop RT-PCR. The miRNA star sequences of 38 known miRNAs and 8 new miRNAs were also discovered, providing additional evidence for the existence of miRNAs. Through degradome sequencing, 145 and 25 genes were identified as targets of annotated miRNAs and new miRNAs, respectively. more...
Organism:
Glycine max
Type:
Non-coding RNA profiling by high throughput sequencing; Expression profiling by high throughput sequencing
Platform:
GPL11192
2 Samples
Download data: TXT
Series
Accession:
GSE25260
ID:
200025260
19.

A diverse set of microRNAs and microRNA-like small RNAs in developing rice grains

(Submitter supplied) Endogenous small RNAs, including microRNAs (miRNAs) and short-interfering RNAs (siRNAs), function as posttranscriptional or transcriptional regulators in plants. miRNA function is essential for normal development and therefore likely to be important in the growth of the rice grain. To investigate the likely roles of miRNAs in rice grain development we carried out deep sequencing of the small RNA populations of rice grains. more...
Organism:
Oryza sativa
Type:
Non-coding RNA profiling by high throughput sequencing
Platforms:
GPL9147 GPL9388
6 Samples
Download data: FNA, QUAL, TXT
Series
Accession:
GSE11014
ID:
200011014
20.

Genome-Wide Identification and Comprehensive Analysis of Conserved MicroRNAs and Phased small Interfering RNAs in Watermelon

(Submitter supplied) This SuperSeries is composed of the SubSeries listed below.
Organism:
Citrullus lanatus
Type:
Other; Non-coding RNA profiling by high throughput sequencing
Platforms:
GPL23842 GPL20225
15 Samples
Download data: TXT
Series
Accession:
GSE102030
ID:
200102030
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